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No / Radio Frequency Identification (RFID) technology has been widely applied in many scenarios such as inventory control, supply chain management due to its superior properties including fast identification and relatively long interrogating range over barcode systems. It is critical to efficiently identify the unknown tags because these tags can appear when new tagged objects are moved in or wrongly placed. The state-of-the-art Basic Unknown tag Identification Protocol-with Collision-Fresh slot paring (BUIP-CF) protocol can first deactivate all the known tags and then collect all the unknown tags. However, BUIP-CF protocol investigates an ALOHA-like technique and causes too many tag responses, which results in low efficiency. This paper proposes a Fast Unknown tag Identification (FUI) protocol which investigates an indicator vector to label the unknown tags with a given accuracy and removes the time-consuming tag responses in the deactivation phase. FUI also adopts the classical Enhanced Dynamic Framed Slotted ALOHA (EDFSA) protocol to collect the labeled unknown tags. We then investigate the optimal parameter settings to maximize the performance of the proposed FUI protocol. Extensive simulation experiments are conducted to evaluate the performance of the proposed FUI protocol and the experimental results show that it considerably outperforms the state-of-the-art protocol.

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Orientador: Zanoni Dias / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Computação / Made available in DSpace on 2018-08-08T07:27:54Z (GMT). No. of bitstreams: 1 Baudet_Christian_M.pdf: 13612079 bytes, checksum: 648d18039dc13dcd5a2f422cc7863666 (MD5) Previous issue date: 2006 / Resumo: O sequenciamento de ESTs (Expressed Sequence Tag) [2] e uma tecnica que trabalha com bibliotecas de cDNAs tendo como objetivo a obtençao de uma boa aproximaçao para o ?ndice genico, que e a listagem de genes existentes no genoma do organismo estudado. Antes da serem analisadas, as sequencias obtidas do sequenciamento dos ESTs devem ser processadas para eliminaçao de artefatos. Artefatos sao trechos que nao pertencem ao organismo ou que possuem baixa qualidade ou baixa complexidade. Trechos de vetores, adaptadores e caudas poli-A podem ser citados como exemplos de artefatos. A eliminaçao dos artefatos deve ser feita para que a an'alise das sequencias produzidas no projeto nao seja prejudicada por estes ?ru?dos?. Por exemplo, artefatos presentes em sequencias freq¨uentemente produzem erros em processos de clusterizaçao, pois eles podem determinar se sequencias serao unidas em um mesmo cluster ou separadas em clusters diferentes. Observando a importancia da realizaçao de um bom processo de limpeza das sequencias, o trabalho desenvolvido nesta dissertaçao teve como principal objetivo a obtençao de um conjunto eficiente de procedimentos de detecçao e remoçao de artefatos. Este conjunto foi produzido a partir de uma nova estrategia de deteçao de artefatos. Normalmente, cada projeto de seq¨uenciamento possui seu proprio conjunto de procedimentos dividido em varias etapas. Estas etapas sao, em geral, ligadas entre si e o resultado de uma pode influenciar o resultado de outra. A nossa estrategia visa a realizaçao destas etapas de forma totalmente independente. Alem da avaliaçao desta nova estrategia, o trabalho tambem realizou um estudo mais detalhado sobre dois tipos de artefatos: baixa qualidade e derrapagem. Para cada um deles, algoritmos foram propostos e validados atraves de testes com conjuntos de seq¨u?encias produzidas em projetos reais de sequenciamento. O conjunto final de procedimentos, baseado nos estudos desenvolvidos durante a escrita deste texto, foi testado com as sequencias do projeto SUCEST [100, 103, 113] e mostrou bons resultados. O clustering produzido com as sequencias processadas por nossos metodos apresentou melhores consistencia interna e externa e menores taxas de redundancia quando comparado ao clustering original do projeto / Abstract: Expressed Sequence Tag (EST) Sequencing [2] is one technique that works with cDNA libraries. It aims to achieve a good approximation for the gene index of an organism. Before analyzing the sequences obtained by sequencing ESTs, they must be processed for artifact removal. An artifact is a sequence that does not belong to the studied organism or that has low quality or low complexity. As example of artifacts, we have adapters, poly- A tails, vectors, etc. Artifacts removal must be performed because their presence can produce ?noises? in the sequencing project data analysis. For example, artifact can join two sequences in a same cluster inappropriately or separate them in two different clusters when they should be put together. Motivated by the sequence cleaning process importance, our main objective in this work was to develop an efficient set of procedures to detect and to remove sequence artifacts. Usually, each EST sequencing project has its own procedure set divided in many steps. These steps are, in general, linked and the result of one given step might influence the result of the next one. Our strategy was to perform each step independently assuring that any execution order of those steps would lead to the same result. Additionally to the new strategy evaluation, this work also studied detailedly two type of artifacts: low quality and slippage. For each one, algorithms were proposed and validated through tests with sequences of real sequencing projects. The final set of procedure, developed in this work, was evaluated using the sequences of the SUCEST project [100, 103, 113] and produced good results. The resulting clustering from our method has better external and internal consistency and lower redundacy rate than those produced by the SUCEST project clustering / Mestrado / Ciência da Computação / Mestre em Ciência da Computação

This thesis examines question tags right and isn't it from pragmatic and sociolinguistic perspectives. English question tags have most frequently been analyzed from the sociolinguistic angle while at the same time completely avoiding the pragmatic aspects that represent a key factor in the sociolinguistic background. The theoretical part of the thesis introduces sociolinguistic aspects and approaches to question tags, as well as their formal aspects. This thesis is a corpus based study (British National Corpus chosen as the primary source of material) where 200 examples were extracted from the corpus and further studied (100 examples on the question tag right and 100 examples on the question tag isn't it). The study analyzes the question tags from the sociolinguistic perspective, focusing on the type of conversation (cross-gender or same-sex conversations) and relating the pragmatic functions of question tags to speakers' gender and speakers' age. Further, the analysis also inquires into what sentence types precede the two question tags. The paper also offers a revised classification of pragmatic functions of the two question tags. Key words: question tags, pragmatic functions of question tags, immediate and postponed response, speaker's gender

Recombinant tags are used extensively in protein expression systems to allow purification through IMAC (Immobilized Metal Affinity Chromatography), identification through Western blot, and to facilitate crystal formation for structural analysis. While widely used, their role in enzyme characterization has raised concerns with respect to potential impact on activity. In this study, a flavonol-specific 3-O glucosyltransferase (Cp3GT) from grapefruit (Citrus paradisi) was expressed in Pichia pastoris, and was assayed in its untagged form and with a C-terminal c-myc/6x His tag under various conditions to determine the effect of tags. Prior characterization of pH optima for Cp3GT obtained through expression in Escherichia coli, containing an N-terminal thioredoxin/6x His tag, indicated an optimal pH of 7–7.5, which is indicative of a normal physiological pH and agrees with other glucosyltransferase (GT) pH optima. However, characterization of Cp3GT expressed using P. pastoris with a C-terminal c-myc-6x His tag showed a higher optimal pH of 8.5–9. This suggests a possible tag effect or an effect related to physiological differences between the cell expression systems. Results testing recombinant Cp3GT expressed in Pichia with and without C-terminal tags showed a possible tag effect with regard to substrate preference and interactions with metals, but no apparent effect on enzymatic kinetics or pH optima.

Radio Frequency Identification (RFID) uses RF radiation to identify physical objects. With decreasing integrated circuit (IC) cost and size, RFID applications are becoming economically feasible and gaining popularity. Researchers at MIT suggest that RFID tags operating in the 900 MHz band (ultrahigh frequency, UHF) represent the best compromise of cost, read range, and capabilities [1]. Passive RFID tags, which exclude radio transmitters and internal power sources, are popular due to their small size and low cost [1]. This project produced Cal Poly’s first ever on-campus printed, assembled, and operational UHF (902 to 928 MHz) passive RFID tag. Project goals include RFID tag antenna design and simulation using the EMPro electromagnetic (EM) simulation tool [47], establishing the tag fabrication process, and testing, operational verification, and comparisons to commercial tag performance. The tag antenna design goal is to meet or exceed the read range performance of the commercial Sirit tag [23] while minimizing the required tag conductive area. This thesis provides an overview of the UHF passive RFID tag fabrication process. Cal Poly’s Graphic Communication Department Laboratory applied a screen‑printing process to print RFID tag antenna patterns onto plastic (PET) substrates. RFID IC-substrate packages were manually attached to tag antennas with conductive adhesives and functionally verified and compared to commercial tag performance. RFID tag antennas were impedance matched (using EMPro) to the Monza 3 RFID IC to maximize IC to antenna power transfer and RFID tag read range.Tag antenna read range (maximum reader-tag communication distance) was characterized in Cal Poly’s Anechoic Chamber, while RFID tag matching characteristics were measured using the differential probe method [33-41] and compared to simulations. Read range results indicate that one of the designs developed in this thesis outperforms a commercial UHF RFID tag.