Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1996. / Includes bibliographical references (leaves 207-220).

Thesis (Ph. D.)--Washington State University, December 2006. / Includes bibliographical references.

Take-all is the most devastating root disease of wheat worldwide. The causal agent is <I>Gaeumannomyces</I> <I>graminis</I> (Sacc.) Arx & Olivier. Based on morphological characteristics and host ranges, three varieties of <I>G</I>. <I>graminis</I> have been recognized. <I>G</I>. <I>graminis</I> var. <I>tritici</I> Walker (Ggt) is the major causal agent of take-all of wheat and barley and the most economically important take-all pathogen. <I>G</I>. <I>graminis</I> var. <I>avenae</I> (Turner) Dennis (Gga) attack oats and causes take-all patch of turf grasses while <I>G</I>. <I>graminis</I> var. <I>graminis</I> (Ggg) is pathogenic on turf grasses but is non-pathogenic on wheat. Conventional diagnosis of take-all pathogens is based on field symptoms such as blackened roots, stunted growth, and white-heads and morphological characteristics such as hyphopodia type, size of perithecia, asci, and ascospores. These procedures are time-consuming, laborious, and often inconclusive. The objective of this study was to develop a rapid, simple, and specific method for differentiation of <I>G</I>. <I>graminis</I> varieties using PCR and molecular-based technology. Exploitation of genes associated with pathogenicity of <I>G</I>. <I>graminis</I> as markers for the test was proposed. Metabolic activities of <I>G</I>. <I>graminis</I> associated with pathogenesis were investigated, namely, the abilities to produce avenacinase and to oxidize manganese. Avenacinase, an avenacin detoxifying enzyme, was associated with Gga pathogenicity for oats but this enzyme is not important in Ggt pathogenicity for wheat. Manganese oxidation was also correlated with Ggt virulence. In this study, avenacinase-like genes were discovered in Ggt and Ggg and manganese oxidation was confirmed for Ggt, Gga, and Ggg. All isolates of Ggt except isolate ATCC 28230 were manganese oxidizers. Ggg and Gga isolates could oxidize manganese but their precipitation patterns were not as intense or closely correlated with mycelial growth as for Ggt. Pathogenicity assays on oats for Ggt, Gga, and Ggg isolates confirmed that Ggt isolates could not cause disease on oats aside from occasional slight root damage. Root weight was reduced for oat seedlings inoculated with Gga isolates. Comparison of partial sequences of avenacinase-like genes from Ggt and Ggg showed strong homology to that of Gga (94.8% identity to Ggt and 94.6% identity to Ggg). However, the Ggt gene was more closely related to that of Ggg (99.2% identity) than to Gga. DNA restriction endonuclease polymorphisms of the genes supported DNA sequencing information and revealed that there were variations within the genes among Ggt, Gga, and Ggg. Variety-specific electrophoretic patterns were obtained when the genes were digested with <I>Hae</I>III. Ggt, Gga, and Ggg upstream (5') variety-specific primers and a downstream (3') universal primer were designed from the avenacinase and avenacinase-like DNA sequences. PCR amplification with Ggt-, Gga-, and Ggg-specific primers generated fragments of 870, 617, and 1,086 bp, respectively. Each 5'-specific primer showed high specificity for its own DNA template in mixed populations of DNA templates. The optimized PCR procedure was sensitive to DNA template concentration as low as 100 pg. Genomic DNA of sixteen Ggt isolates, seven Gga isolates, and five Ggg isolates were tested. Although all Ggt isolates were originally isolated from wheat, seven isolates produced Ggg-specific fragments. This result corresponded well with <I>Hae</I>III DNA polymorphisms, pathogenicity assay, and manganese oxidizing ability. All but one Gga isolates produced the variety-specific fragment. Ggt- and Gga- specific products were generated from Gga isolate RB-W. Although Ggg-specific fragments were produced from all Ggg isolates, non-specific products were also observed from isolates that were not from wheat origin suggesting some genetic variations due to host ranges. Additionally, no non-specific amplification was obtained from any closely related fungi such as <I>Gaeumannomyces</I> <I>cylindrosporus</I> or <I>Phialophora</I> spp. The test developed in this study is the first test capable of identification of Ggt, Gga, and Ggg in a single PCR tube with a basic PCR protocol. The test is rapid and specific. Interpretation of results is simple and conclusive based on differences in size of each variety-specific fragment. / Ph. D.

The Winner-Take-All (WTA) is an essential neural network operation for locating the most active neuron. Such a procedure has been extensively used in larger application areas. The Winner-Take-All circuit selects the maximum of the inputs inhibiting all other nodes. The efficiency of the analog circuits may well be considerably higher than the digital circuits. Also, analog circuits’ design footprint and processing time can be significantly small. A current-based Winner-Take-All circuit for analog neural networks is presented in this research. A compare and pass (CAP) mechanism has been used, where each input pair is compared, and the winner is selected and passed to another level. The inputs are compared by a sense amplifier which generates high and low voltage signals at the output node. The voltage signal of the sense amplifier is used to select the winner and passed to another level using logic gates. Also, each winner follows a sequence of digital bits to be selected. The findings of the SPICE simulation are also presented. The simulation results on the MNIST, Fashion-MNIST, and CIFAR10 datasets for the memristive deep neural network model show the significantly accurate result of the winner class with an average difference of input and selected winner output current of 0.00795uA, 0.01076uA and 0.02364uA respectively. The experimental result with transient noise analysis is also presented.

Includes bibliographical references. / xiv, 210 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1995

Includes bibliographical references.

Bibliography: leaves 82-89. This study is aimed at providing information on control measures against Gaeumannomyces graminis var. tritici (abbreviated to Ggt) in annual pastures across southern Australia. The impact of timing of herbicide application, the impact of variation in timing of rainfall patterns, as well as herbicide application on the control of Ggt are also discussed. Additional experiments examine the ability of grass genera to host and carry over Ggt.

Artificial neural networks are widely used for parallel processing of data analysis and visual information. The most prominent example of artificial neural networks is a cellular neural network (CNN), composed from two-dimensional arrays of simple first-order dynamical systems (“cells”) that are interconnected by wires. The information, to be processed by a CNN, represents the initial state of the network, and the parallel information processing is performed by converging to one of the stable spatial equilibrium states of the multi-stable CNN. This thesis studies a specific type of CNNs designed to perform the winner-take-all function of finding the largest among the n numbers, using the network dynamics. In a wider context, this amounts to automatically detecting a target spot in the given visual picture. The research, reported in this thesis, demonstrates that the addition of fast on-off switching (blinking) connections significantly improves the functionality of winner-take-all CNNs. Numerical calculations are performed to reveal the dependence of the probability, that the CNN correctly classifies the largest number, on the switching frequency.

Recently, we observed many networked market are served almost by a single platform, and can called that situation be winner-take-all. We could observed that the top website occupy huge market, and still be the top for long time. Conversely, the other website only shares the tiny market. This paper chooses the biggest Taiwan portal Yahoo!Kimo to be the research case. In addition, the case object is its e-commerce department, which is the Yahoo! only e-commerce department worldwide. Through this case, we could find out the truth of winner-take-all. In this research, we use in-depth interview and combine with secondary data. According to past researches and the situation of Yahoo!Kimo, we address the research question as follow: (1) How does Yahoo!Kimo develop their e-store platforms to maintain their e-commerce leading position in Taiwan. (2) Is there a winner-take-all phenomenon on Yahoo!Kimo? After generalize the data, we found that although Yahoo! first time enters Taiwan not successful. Their strategy is merge different kinds website, such as the leading portal Kimo, the most visiting blog Wretch, and Monday.Tech. They got their human resource and technology sooner, then become the top one portal in Taiwan provides variety services for users. For foreign website and companies enter a new market will be a good role model. Moreover, we prove Yahoo!Kimo is role model of winner-take-all.

Bibliography: leaves 141-145 / vii, 145 leaves, [4] leaves of plates : ill. (4 col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Depts. of Plant Pathology and Agronomy, 1986