No abstract available / Dissertation (MA)--University of Pretoria, 1963. / gm2013 / Psychology / unrestricted

Major depression is a serious mood disorder affecting more than 120 million people worldwide, irrespective of their race or socio-economic status. This psychiatric disorder is predicted to become the second leading cause of disability by the year 2020, second only to heart diseases in the global population, without distinguishing differences in the incidence within defined age groups. Depression is known to affect people across all age groups, including children, adolescents, adults and geriatrics, although older age is associated with an increased susceptibility to major depression and other psychiatric conditions. Until the 1970‘s depression during childhood and adolescence was thought to be uncommon or non-existent. Recent epidemiological studies have demonstrated that there is a persistent escalation in the prevalence of depression in children and adolescents. Accordingly, the number of prescriptions for drugs to treat this disorder in juveniles has escalated significantly. With our current limited understanding of the safety and long-term effects of treatment with antidepressants, the clinician is left making decisions without sound evidence of safety. In addition, psychotropic drugs may affect neurodevelopment during childhood and adolescence and may consequently modulate susceptibility to psychiatric disorders later in life. The objective of the current study was to investigate the effects of early-life (pre-natal and postnatal) chronic treatment with venlafaxine, a dual action serotonin-noradrenalin reuptake inhibitor, during the developmental phase of the serotonin and norepinephrine pathways in stress-sensitive rats on measures of cognition, anxiety-like and depressive-like behaviour later in life. The study also investigated which age shows optimal behavioural changes later in life, following the above mentioned administration of venlafaxine. In addition we also determined the effects that the administration of venlafaxine has on the levels of monoamines l-norepinephrine (l-NE) and serotonin (5-HT) in the prefrontal cortex and the hippocampus. A number of translational animal models of psychiatric disorders have been described and validated, and is suitable for such investigations. For the current study we used stress-sensitive Flinders Sensitive Line (FSL) rats and their controls, Flinders Resistant Line (FRL) rats. Pregnant dams were injected subcutaneously for 14 days with 10 mg/kg venlafaxine or saline from pre-natal day 15 (ND-15) to ND-01. New-born pups were then injected subcutaneously with 3 mg/kg venlafaxine or saline for 14 days from postnatal day 3 (ND+03) to ND+17. These doses were determined from previous studies reported in literature. Four rat treatment groups of both FSL and FRL rats received injections during pre-natal + postnatal ages as follows: saline + saline, venlafaxine + saline, saline + venlafaxine and venlafaxine + venlafaxine. Following the drug treatments, all rat groups were housed under normal conditions until the indicated time to be subjected to a battery of behavioural tests, including the novel object recognition test (nORT), locomotor activity test (Digiscan®), elevated plus maze (EPM) and forced-swim test (FST), scheduled on either ND+35, ND+60 or ND+90. Separate treatment groups were used for each age group. After the behavioural tests animals were decapitated, the brains removed and the prefrontal cortex and hippocampus dissected out. These were analysed at a later stage using an HPLC with electrochemical detection to determine the levels of the monoamines l-NE and 5-HT. All animal procedures were approved by the Ethics Committee of the North-West University (approval number: NWU-00045-10-S5), and are in accordance with the recommendations of the National Institutes of Health guide for the care and use of laboratory animals. The data from the current study suggest that in general FRL rats were not influenced by the early-life treatment with venlafaxine, as observed in the nORT, EPM or FST on ND+35, ND+60 or ND+90. There was minimal changes seen in the immobile behaviour in the FST of FRL rats that received prenatal venlafaxine. As expected, depressive-like behaviour in the FST was significantly enhanced in FSL rats relative to corresponding FRL rat groups as observed at ND+35 and ND+60, but not ND+90. Importantly, depressive-like behaviour was reversed following pre- and postnatal treatment with venlafaxine in FSL rats at ND+60, relative to the corresponding FRL rat groups. Reversal of depressive-like behaviour in FSL rats were not observed at ND+35 or ND+90, suggesting a delayed response that is reversed later in adulthood. The data from the nORT, Digiscan® or EPM did not reveal any significant differences between the various FSL treatment groups, including at ND+60. The current study therefore demonstrated that the treatment regimen employed had a transient effect on depressive-like behaviour later in life and suggested that genetic susceptibility plays an important role in the treatment of depression. This was suggested by the venlafaxine-induced decrease in immobile behaviour exhibited by FSL rats at ND+60 in the FST, and the subsequent increase in immobile behaviour at ND+90. In general, the most significant venlafaxine-induced effects were seen in FSL rats, suggesting genetic susceptibility plays an important role. / MSc (Pharmacology), North-West University, Potchefstroom Campus, 2014

Major depression is a serious mood disorder affecting more than 120 million people worldwide, irrespective of their race or socio-economic status. This psychiatric disorder is predicted to become the second leading cause of disability by the year 2020, second only to heart diseases in the global population, without distinguishing differences in the incidence within defined age groups. Depression is known to affect people across all age groups, including children, adolescents, adults and geriatrics, although older age is associated with an increased susceptibility to major depression and other psychiatric conditions. Until the 1970‘s depression during childhood and adolescence was thought to be uncommon or non-existent. Recent epidemiological studies have demonstrated that there is a persistent escalation in the prevalence of depression in children and adolescents. Accordingly, the number of prescriptions for drugs to treat this disorder in juveniles has escalated significantly. With our current limited understanding of the safety and long-term effects of treatment with antidepressants, the clinician is left making decisions without sound evidence of safety. In addition, psychotropic drugs may affect neurodevelopment during childhood and adolescence and may consequently modulate susceptibility to psychiatric disorders later in life. The objective of the current study was to investigate the effects of early-life (pre-natal and postnatal) chronic treatment with venlafaxine, a dual action serotonin-noradrenalin reuptake inhibitor, during the developmental phase of the serotonin and norepinephrine pathways in stress-sensitive rats on measures of cognition, anxiety-like and depressive-like behaviour later in life. The study also investigated which age shows optimal behavioural changes later in life, following the above mentioned administration of venlafaxine. In addition we also determined the effects that the administration of venlafaxine has on the levels of monoamines l-norepinephrine (l-NE) and serotonin (5-HT) in the prefrontal cortex and the hippocampus. A number of translational animal models of psychiatric disorders have been described and validated, and is suitable for such investigations. For the current study we used stress-sensitive Flinders Sensitive Line (FSL) rats and their controls, Flinders Resistant Line (FRL) rats. Pregnant dams were injected subcutaneously for 14 days with 10 mg/kg venlafaxine or saline from pre-natal day 15 (ND-15) to ND-01. New-born pups were then injected subcutaneously with 3 mg/kg venlafaxine or saline for 14 days from postnatal day 3 (ND+03) to ND+17. These doses were determined from previous studies reported in literature. Four rat treatment groups of both FSL and FRL rats received injections during pre-natal + postnatal ages as follows: saline + saline, venlafaxine + saline, saline + venlafaxine and venlafaxine + venlafaxine. Following the drug treatments, all rat groups were housed under normal conditions until the indicated time to be subjected to a battery of behavioural tests, including the novel object recognition test (nORT), locomotor activity test (Digiscan®), elevated plus maze (EPM) and forced-swim test (FST), scheduled on either ND+35, ND+60 or ND+90. Separate treatment groups were used for each age group. After the behavioural tests animals were decapitated, the brains removed and the prefrontal cortex and hippocampus dissected out. These were analysed at a later stage using an HPLC with electrochemical detection to determine the levels of the monoamines l-NE and 5-HT. All animal procedures were approved by the Ethics Committee of the North-West University (approval number: NWU-00045-10-S5), and are in accordance with the recommendations of the National Institutes of Health guide for the care and use of laboratory animals. The data from the current study suggest that in general FRL rats were not influenced by the early-life treatment with venlafaxine, as observed in the nORT, EPM or FST on ND+35, ND+60 or ND+90. There was minimal changes seen in the immobile behaviour in the FST of FRL rats that received prenatal venlafaxine. As expected, depressive-like behaviour in the FST was significantly enhanced in FSL rats relative to corresponding FRL rat groups as observed at ND+35 and ND+60, but not ND+90. Importantly, depressive-like behaviour was reversed following pre- and postnatal treatment with venlafaxine in FSL rats at ND+60, relative to the corresponding FRL rat groups. Reversal of depressive-like behaviour in FSL rats were not observed at ND+35 or ND+90, suggesting a delayed response that is reversed later in adulthood. The data from the nORT, Digiscan® or EPM did not reveal any significant differences between the various FSL treatment groups, including at ND+60. The current study therefore demonstrated that the treatment regimen employed had a transient effect on depressive-like behaviour later in life and suggested that genetic susceptibility plays an important role in the treatment of depression. This was suggested by the venlafaxine-induced decrease in immobile behaviour exhibited by FSL rats at ND+60 in the FST, and the subsequent increase in immobile behaviour at ND+90. In general, the most significant venlafaxine-induced effects were seen in FSL rats, suggesting genetic susceptibility plays an important role. / MSc (Pharmacology), North-West University, Potchefstroom Campus, 2014

The research question addressed by this dissertation is: How is the tourist experience formed and what constitutes the authenticity of the tourist experience for two market segments (motivated and not motivated by learning) of tourists visiting (political) cultural heritage sites in South Africa. The study explores the correlation between three types of authenticity, namely objective, constructed and existential on two independent tourist samples, motivated and not motivated by learning. This research was initiated for three reasons. The first reason forms part of the research problem; South African cultural experiences received the lowest ratings from the international tourists despite the fact that culture and heritage play a role in reimaging South Africa from Big 5 destination into ‘It’s possible’ and ‘Leave ordinary behind’. It was suspected that not all types of cultural heritage products justify such a low ratings, especially not the political cultural heritage sites South Africa is famous for. The second reason emerged from the academic literature on authenticity theories and calls from the influential group of postmodernist scholars to declare the objective authenticity obsolete and replace it with the existential authenticity. The argument that; the hyperreal nature of the postmodern experience and its detachment from reality makes the authenticity of the site redundant, seemed inapt for cultural heritage sites exclusively dependent on their historical and authentic values. The third reason was the inability of the postmodern paradigm to explain the new tourism phenomenon driven by the tourists search for selfdevelopment through authentic experiences. The new emerging paradigm, transmodernity seemed to offer better theoretical framework in explaining the omnivorouessness of tourists’ consumption and the authentic nature of tourist experiences. The correlational character of the research question required a descriptive correlational design and quantitative methodology. The selected research instrument for primary data collection is a self–administered questionnaire. The sampling strategy is a non–probability sampling, and the sampling method is a convenience or accidental sample. The data was collected from November 2010 to February 2011 at the Constitutional Hill National Heritage Site in Johannesburg. The final sample (436) consists of 254 foreign and 182 domestic tourists. The questionnaire was designed to identify the variables pertinent to each type of authenticity of tourists experience and of the resultant tourist experience. The data analysis provided very interesting results. Firstly, the results of crosstabulation proved that more than half (56%) of the tourists expressed strong agreement that the Constitution Hill provided them with authentic experience, hence a proof that political heritage sites are not responsible for the overall low experiential ratings of the country’s culture and heritage. Secondly, the results of the Spearman’s correlation coefficient proved that objective authenticity as an independent variable have strong positive correlation with constructed and existential authenticity hence a proof that objective authenticity cannot be declared obsolete and replaced with existential authenticity. Finally, the results of the t–test proved that motivation for learning and place of birth do not play an important role in how tourist evaluate and experience the authenticity of the site pointing to the omnivorous nature of tourist consumption. In line with the transmodern paradigm, motivation for learning plays a critical role in triggering the transformative, authentic experience distinctive of the existential authenticity. The results of the study also showed that 32% of tourists are in fact the purposeful, New Age, transmodern Cultural Creatives. Proposed theoretical model of authenticity of tourists experience presents a theoretical platform for future research studies. / Thesis (M.A. (Tourism))--North-West University, Potchefstroom Campus, 2012.

The research question addressed by this dissertation is: How is the tourist experience formed and what constitutes the authenticity of the tourist experience for two market segments (motivated and not motivated by learning) of tourists visiting (political) cultural heritage sites in South Africa. The study explores the correlation between three types of authenticity, namely objective, constructed and existential on two independent tourist samples, motivated and not motivated by learning. This research was initiated for three reasons. The first reason forms part of the research problem; South African cultural experiences received the lowest ratings from the international tourists despite the fact that culture and heritage play a role in reimaging South Africa from Big 5 destination into ‘It’s possible’ and ‘Leave ordinary behind’. It was suspected that not all types of cultural heritage products justify such a low ratings, especially not the political cultural heritage sites South Africa is famous for. The second reason emerged from the academic literature on authenticity theories and calls from the influential group of postmodernist scholars to declare the objective authenticity obsolete and replace it with the existential authenticity. The argument that; the hyperreal nature of the postmodern experience and its detachment from reality makes the authenticity of the site redundant, seemed inapt for cultural heritage sites exclusively dependent on their historical and authentic values. The third reason was the inability of the postmodern paradigm to explain the new tourism phenomenon driven by the tourists search for selfdevelopment through authentic experiences. The new emerging paradigm, transmodernity seemed to offer better theoretical framework in explaining the omnivorouessness of tourists’ consumption and the authentic nature of tourist experiences. The correlational character of the research question required a descriptive correlational design and quantitative methodology. The selected research instrument for primary data collection is a self–administered questionnaire. The sampling strategy is a non–probability sampling, and the sampling method is a convenience or accidental sample. The data was collected from November 2010 to February 2011 at the Constitutional Hill National Heritage Site in Johannesburg. The final sample (436) consists of 254 foreign and 182 domestic tourists. The questionnaire was designed to identify the variables pertinent to each type of authenticity of tourists experience and of the resultant tourist experience. The data analysis provided very interesting results. Firstly, the results of crosstabulation proved that more than half (56%) of the tourists expressed strong agreement that the Constitution Hill provided them with authentic experience, hence a proof that political heritage sites are not responsible for the overall low experiential ratings of the country’s culture and heritage. Secondly, the results of the Spearman’s correlation coefficient proved that objective authenticity as an independent variable have strong positive correlation with constructed and existential authenticity hence a proof that objective authenticity cannot be declared obsolete and replaced with existential authenticity. Finally, the results of the t–test proved that motivation for learning and place of birth do not play an important role in how tourist evaluate and experience the authenticity of the site pointing to the omnivorous nature of tourist consumption. In line with the transmodern paradigm, motivation for learning plays a critical role in triggering the transformative, authentic experience distinctive of the existential authenticity. The results of the study also showed that 32% of tourists are in fact the purposeful, New Age, transmodern Cultural Creatives. Proposed theoretical model of authenticity of tourists experience presents a theoretical platform for future research studies. / Thesis (M.A. (Tourism))--North-West University, Potchefstroom Campus, 2012.

A uniplanar balun that transforms unbalanced coplanar waveguide (CPW) to balanced coplanar strip line (CPS) is characterised through simulation and measurement. By illustrating the effect of many of the critical design parameters, the operation of this balun is discussed and a set of design criteria is defined. The parameter study discusses the size and shape of the radial open, the type and length of the CPW taper and the thickness and type of the bond wires. Newly developed etched bond wires are implemented to provide better manufacturing repeatability and reliability. A complete balun testing procedure is developed and described, consisting of three separate tests. The balun is tested in the normal back-to-back configuration, as a terminated single balun, and the magnitude and phase imbalance is also determined by using a three-port test circuit connected to the balun. The advantages of implementing this full test procedure, and thus fully characterising the balun under test, are emphasised throughout. Results obtained by using this procedure show that the basic balun works well over the full operating band, except for the phase imbalance, which is usable but not optimal. A simple technique to correct the phase imbalance of the balun is introduced, and validated through measurements of the balun connected to the three-port test circuit. As a final validation the balun is connected as feed for an etched dipole antenna for which good impedance matching and pattern results are shown. AFRIKAANS : ’n Enkelvlak balon (BALans-na-ONbalans) wat van ongebalanseerde enkelvlak golfgeleier (CPW) na gebalanseerde enkelvlak strooklyn (CPS) transformeer, word gekarakteriseer deur simulasie en metings. Deur die effek van baie van die kritiese ontwerpsparameters te demonstreer, word die werking van die balon bespreek en ’n stel ontwerpskriteria opgestel. Die parameter studie bespreek die radiale ope struktuur se vorm en grote, die tipe en lengte van die CPW transformator and die dikte en tipe van die konneksie drade. Nuut ontwikkelde geëtste konneksie drade word geïmplementeer om beter vervaardigingsherhaalbaarheid en betroubaarheid te verseker. ’n Volledige balon toetsprosedure word ontwikkel en beskryf en bestaan uit drie aparte toetse. Die balon word getoets in die normale rug-aan-rug konfigurasie, as ’n enkel getermineerde balon en die grote asook fase van die wanbalans word bepaal deur die gebruik van ’n drie-poort toetsbaan wat aan die balon gekoppel word. Die voordele verbonde daaraan om hierdie volledige toetsprosedure toe te pas, en daardeur die balon volledig te karakteriseer, word deurlopend beklemtoon. Die resultate wat hierdie prosedure oplewer wys dat die basiese balon goed werk oor die volledige frekwensieband, behalwe vir die fase-wanbalans parameter, wat bruikbaar, maar nie ideaal is nie. ’n Eenvoudige tegniek om die fase-wanbalans van die balon te korrigeer word bekend gestel en getoets deur die drie-poort toetsbaan weer te gebruik. As ’n finale validasie word die balon aan ’n geëtste dipool gekoppel word, waarvan goeie impedansie aanpassings en patrone gewys word. Copyright / Dissertation (MEng)--University of Pretoria, 2010. / Electrical, Electronic and Computer Engineering / unrestricted

The evolution of aerobic metabolic processes unavoidably led to the production of reactive oxygen species (ROS). ROS have the ability to cause harmful oxidative damage to biomolecules. Increased ROS generation and subsequent oxidative stress have been associated with aging and neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases as a result of the extreme sensitivity of the central nervous system to damage from ROS. Antioxidant defence systems have co–evolved with aerobic metabolic processes to counteract oxidative damage inflicted by ROS. The impact of neurodegenerative disorders on society is increasing rapidly as the life expectancy of the global population increases. In this day and age, a much younger group of the population is also experiencing neurodegenerative symptoms as a result of the harmful effect of the human immunodeficiency virus (HIV) on the central nervous system. Plants are an invaluable source of medicinal compounds. The use of plants for their healing properties is rooted in ancient times. The aim of this study was to select from twenty one plants, the plant with the most promising antioxidant activity and to determine whether extracts of this plant could act as free radical scavengers, comparing the results to Trolox, a known free radical scavenger. The next step was to isolate and characterize a compound from an extract exhibiting promising antioxidant activity. Bioassay–guided fractionation was followed to achieve this. During screening trials, twenty one plants, namely Berula erecta, Heteromorpha arborescens, Tarchonanthus camphoratus, Vernonia oligocephala, Gymnosporia buxifolia, Acacia karroo, Elephantorrhiza elephantina, Erythrina zeyheri, Leonotis leonurus, Plectranthus ecklonii, P. rehmanii, P. venteri, Salvia auretia, S. runcinata, Solenostemon latifolius, S. rotundifolius, Plumbago auriculata, Clematis brachiata, Vangueria infausta, Physalis peruviana and Lippia javanica were selected from literature, based on reported antioxidant activity within the plant families, for screening of their antioxidant activity. One hundred and ten extracts were prepared from the leaves, using Soxhlet extraction and the solvents petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH), consecutively. The focus during initial screening trials was on chemistry–based assays. The oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays were employed for the primary screening of the one hundred and ten leaf extracts. The ORAC assay was used to determine whether the plant extracts were able to scavenge peroxyl radicals and the FRAP assay was used to determine the reducing abilities of the extracts. Quantification of the peroxyl radical scavenging activity by the ORAC assay revealed that activity was observed for most of the extracts, with the ethyl acetate and ethanol extracts of L. javanica exhibiting the most promising activity. This pattern of activity was also found with the reducing capacity evaluated by the FRAP assay in which the EtOAc and EtOH extracts of L. javanica also exhibited the most promising activity. L. javanica was selected for further study by screening for biological activity, employing the nitro–blue tetrazolium (NBT) assay and thiobarbituric acid reactive substances (TBARS) assay. Using a cyanide model to induce neurotoxic effects in rat brain homogenate, the neuroprotective properties of the extracts of L. javanica leaves were examined using the NBT assay and compared to that of Trolox. The NBT assay determines the level of superoxide anions. All the extracts of L. javanica significantly reduced superoxide anion generation at all concentrations used. The petroleum ether and ethyl acetate extracts, at all concentrations, reduced superoxide anion generation to values lower than that of the control, suggesting that these extracts may be able to attenuate normal free radical processes in the brain. The petroleum ether extract exhibited the most promising activity at a concentration of 1.25 and 2.5 mg/ml and also exhibited similar results as the ethyl acetate extract at a lower concentration than the ethyl acetate extract (2.5 mg/ml compared to 5 mg/ml). A toxin–solution consisting of hydrogen peroxide (H2O2), iron(III)chloride (FeCl3) and ascorbic acid was used to induce lipid peroxidation and the ability of the extracts of the leaves of L. javanica to attenuate lipid peroxidation was investigated in rat brain homogenate and compared to that of Trolox. All of the extracts of L. javanica significantly attenuated toxininduced lipid peroxidation at all concentrations used. All of the extracts were also able to significantly attenuate toxin–induced lipid peroxidation to values lower than that of the control. These results suggest that all of the extracts of L. javanica possess the ability to attenuate not only toxin–induced lipid peroxidation, but also lipid peroxidation that occurs during normal processes in the brain. The petroleum ether extract was subjected to bioassay–guided fractionation using column and thin–layer chromatography and the NBT and TBARS assays. Fraction DD1 was investigated by means of nuclear magnetic resonance, infrared and mass spectrometry. The exact structure of fraction DD1 was not elucidated. Considering all the results, it is clear that L. javanica shows great potential as a medicinal plant with antioxidant activity and may therefore be beneficial in diminishing the destructive oxidative effects inflicted by free radicals. There are however still many compounds to be isolated from L. javanica. Key words: Verbenaceae, Lippia javanica, antioxidant, neurodegeneration, oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), nitro–blue tetrazolium assay (NBT), thiobarbituric acid reactive substances assay (TBARS). / Thesis (M.Sc. (Pharmaceutical Chemistry))--North-West University, Potchefstroom Campus, 2011.

The evolution of aerobic metabolic processes unavoidably led to the production of reactive oxygen species (ROS). ROS have the ability to cause harmful oxidative damage to biomolecules. Increased ROS generation and subsequent oxidative stress have been associated with aging and neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases as a result of the extreme sensitivity of the central nervous system to damage from ROS. Antioxidant defence systems have co–evolved with aerobic metabolic processes to counteract oxidative damage inflicted by ROS. The impact of neurodegenerative disorders on society is increasing rapidly as the life expectancy of the global population increases. In this day and age, a much younger group of the population is also experiencing neurodegenerative symptoms as a result of the harmful effect of the human immunodeficiency virus (HIV) on the central nervous system. Plants are an invaluable source of medicinal compounds. The use of plants for their healing properties is rooted in ancient times. The aim of this study was to select from twenty one plants, the plant with the most promising antioxidant activity and to determine whether extracts of this plant could act as free radical scavengers, comparing the results to Trolox, a known free radical scavenger. The next step was to isolate and characterize a compound from an extract exhibiting promising antioxidant activity. Bioassay–guided fractionation was followed to achieve this. During screening trials, twenty one plants, namely Berula erecta, Heteromorpha arborescens, Tarchonanthus camphoratus, Vernonia oligocephala, Gymnosporia buxifolia, Acacia karroo, Elephantorrhiza elephantina, Erythrina zeyheri, Leonotis leonurus, Plectranthus ecklonii, P. rehmanii, P. venteri, Salvia auretia, S. runcinata, Solenostemon latifolius, S. rotundifolius, Plumbago auriculata, Clematis brachiata, Vangueria infausta, Physalis peruviana and Lippia javanica were selected from literature, based on reported antioxidant activity within the plant families, for screening of their antioxidant activity. One hundred and ten extracts were prepared from the leaves, using Soxhlet extraction and the solvents petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH), consecutively. The focus during initial screening trials was on chemistry–based assays. The oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays were employed for the primary screening of the one hundred and ten leaf extracts. The ORAC assay was used to determine whether the plant extracts were able to scavenge peroxyl radicals and the FRAP assay was used to determine the reducing abilities of the extracts. Quantification of the peroxyl radical scavenging activity by the ORAC assay revealed that activity was observed for most of the extracts, with the ethyl acetate and ethanol extracts of L. javanica exhibiting the most promising activity. This pattern of activity was also found with the reducing capacity evaluated by the FRAP assay in which the EtOAc and EtOH extracts of L. javanica also exhibited the most promising activity. L. javanica was selected for further study by screening for biological activity, employing the nitro–blue tetrazolium (NBT) assay and thiobarbituric acid reactive substances (TBARS) assay. Using a cyanide model to induce neurotoxic effects in rat brain homogenate, the neuroprotective properties of the extracts of L. javanica leaves were examined using the NBT assay and compared to that of Trolox. The NBT assay determines the level of superoxide anions. All the extracts of L. javanica significantly reduced superoxide anion generation at all concentrations used. The petroleum ether and ethyl acetate extracts, at all concentrations, reduced superoxide anion generation to values lower than that of the control, suggesting that these extracts may be able to attenuate normal free radical processes in the brain. The petroleum ether extract exhibited the most promising activity at a concentration of 1.25 and 2.5 mg/ml and also exhibited similar results as the ethyl acetate extract at a lower concentration than the ethyl acetate extract (2.5 mg/ml compared to 5 mg/ml). A toxin–solution consisting of hydrogen peroxide (H2O2), iron(III)chloride (FeCl3) and ascorbic acid was used to induce lipid peroxidation and the ability of the extracts of the leaves of L. javanica to attenuate lipid peroxidation was investigated in rat brain homogenate and compared to that of Trolox. All of the extracts of L. javanica significantly attenuated toxininduced lipid peroxidation at all concentrations used. All of the extracts were also able to significantly attenuate toxin–induced lipid peroxidation to values lower than that of the control. These results suggest that all of the extracts of L. javanica possess the ability to attenuate not only toxin–induced lipid peroxidation, but also lipid peroxidation that occurs during normal processes in the brain. The petroleum ether extract was subjected to bioassay–guided fractionation using column and thin–layer chromatography and the NBT and TBARS assays. Fraction DD1 was investigated by means of nuclear magnetic resonance, infrared and mass spectrometry. The exact structure of fraction DD1 was not elucidated. Considering all the results, it is clear that L. javanica shows great potential as a medicinal plant with antioxidant activity and may therefore be beneficial in diminishing the destructive oxidative effects inflicted by free radicals. There are however still many compounds to be isolated from L. javanica. Key words: Verbenaceae, Lippia javanica, antioxidant, neurodegeneration, oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), nitro–blue tetrazolium assay (NBT), thiobarbituric acid reactive substances assay (TBARS). / Thesis (M.Sc. (Pharmaceutical Chemistry))--North-West University, Potchefstroom Campus, 2011.

Lower body explosive power (LBEP) forms a critical component in any individual and team sport performance and it is therefore essential to develop a means of predicting LBEP in adolescents for early identification of future talent in various sporting codes. LBEP is frequently used by athletes during matches or competitions where explosive movements such as jumping, agility running and sprinting are required for successful performance. These movements are usually found in individual sports such as long jump and high jump as well as in team sports such as basketball, volleyball and soccer. To date not much literature is available on LBEP, especially with regard to LBEP prediction models. Furthermore, studies on adolescents are scarce and a LBEP prediction model has not yet been developed for a South African adolescent population. It is against this background that the objectives of this study were firstly, to develop a LBEP prediction model from various physical and motor performance components among a cohort of adolescents living in the Tlokwe local municipality of Dr Kenneth Kaunda district in the North-West Province, South Africa; and secondly, to develop a LBEP prediction model from several anthropometric measurements among a cohort of male and female adolescents living in the Tlokwe local municipality of Dr Kenneth Kaunda district in the North-West Province, South Africa. Two hundred and fourteen (15.8±0.68 years) 15-year-old adolescents (126 females, 88 males) from 6 surrounding schools within the Tlokwe local municipality of Dr Kenneth Kaunda district in the North-West Province of South Africa were purposefully selected from pre-acquired class lists took part in the study. Data was collected by means of various questionnaires as well as anthropometrical, physical and motor performance tests. For representation of LBEP a principal component factor analysis was done and the results indicated that the vertical jump test (VJT) was the best indicator of LBEP in the cohort of adolescents. With regard to the anthropometrical related LBEP prediction model, the forward stepwise regression analysis yielded a correlation coefficient of R2 = 0.69. The following variables contributed significantly (p≤0.001) to the anthropometrical LBEP prediction model: stature (57%), muscle mass percentage (10%) and maturity age (3%). The LBEP prediction model that was developed equated to LBEP (vertical jump) = -136.30 + 0.84(stature) + 0.7(muscle mass percentage) + 4.6(maturity age). Variables other than the variables that formed part of the study could explain the further 31% variance in the LBEP of the adolescents. The physical and motor performance LBEP prediction model indicated that gender (39%) and 10 m speed (7%) contributed significantly (p ≤ 0.001) to the overall prediction of the LBEP of the adolescents. The LBEP prediction model delivered a stepwise forward regression analysis coefficient of R2=0.458 and a prediction formula LBEP = 68.21 + 9.82 (gender) – 18.33(10 m speed). The remaining 56% of the variance in the results could be explained by other factors than the variables considered in the study. In conclusion, to the best of the researchers’ knowledge, this is the first study which has made an attempt at developing LBEP prediction models from the anthropometrical, physical and motor performance components of a cohort of adolescents of South Africa. The prediction models developed in the study will assist teachers sport scientists and sporting coaches who have limited resources available, to measure and calculate LBEP in adolescents, with the means to do so in South Africa. Further high quality studies are necessary to further improve and develop such prediction models for various age groups of adolescents in the greater South Africa. / MSc (Sport Science), North-West University, Potchefstroom Campus, 2014

Lower body explosive power (LBEP) forms a critical component in any individual and team sport performance and it is therefore essential to develop a means of predicting LBEP in adolescents for early identification of future talent in various sporting codes. LBEP is frequently used by athletes during matches or competitions where explosive movements such as jumping, agility running and sprinting are required for successful performance. These movements are usually found in individual sports such as long jump and high jump as well as in team sports such as basketball, volleyball and soccer. To date not much literature is available on LBEP, especially with regard to LBEP prediction models. Furthermore, studies on adolescents are scarce and a LBEP prediction model has not yet been developed for a South African adolescent population. It is against this background that the objectives of this study were firstly, to develop a LBEP prediction model from various physical and motor performance components among a cohort of adolescents living in the Tlokwe local municipality of Dr Kenneth Kaunda district in the North-West Province, South Africa; and secondly, to develop a LBEP prediction model from several anthropometric measurements among a cohort of male and female adolescents living in the Tlokwe local municipality of Dr Kenneth Kaunda district in the North-West Province, South Africa. Two hundred and fourteen (15.8±0.68 years) 15-year-old adolescents (126 females, 88 males) from 6 surrounding schools within the Tlokwe local municipality of Dr Kenneth Kaunda district in the North-West Province of South Africa were purposefully selected from pre-acquired class lists took part in the study. Data was collected by means of various questionnaires as well as anthropometrical, physical and motor performance tests. For representation of LBEP a principal component factor analysis was done and the results indicated that the vertical jump test (VJT) was the best indicator of LBEP in the cohort of adolescents. With regard to the anthropometrical related LBEP prediction model, the forward stepwise regression analysis yielded a correlation coefficient of R2 = 0.69. The following variables contributed significantly (p≤0.001) to the anthropometrical LBEP prediction model: stature (57%), muscle mass percentage (10%) and maturity age (3%). The LBEP prediction model that was developed equated to LBEP (vertical jump) = -136.30 + 0.84(stature) + 0.7(muscle mass percentage) + 4.6(maturity age). Variables other than the variables that formed part of the study could explain the further 31% variance in the LBEP of the adolescents. The physical and motor performance LBEP prediction model indicated that gender (39%) and 10 m speed (7%) contributed significantly (p ≤ 0.001) to the overall prediction of the LBEP of the adolescents. The LBEP prediction model delivered a stepwise forward regression analysis coefficient of R2=0.458 and a prediction formula LBEP = 68.21 + 9.82 (gender) – 18.33(10 m speed). The remaining 56% of the variance in the results could be explained by other factors than the variables considered in the study. In conclusion, to the best of the researchers’ knowledge, this is the first study which has made an attempt at developing LBEP prediction models from the anthropometrical, physical and motor performance components of a cohort of adolescents of South Africa. The prediction models developed in the study will assist teachers sport scientists and sporting coaches who have limited resources available, to measure and calculate LBEP in adolescents, with the means to do so in South Africa. Further high quality studies are necessary to further improve and develop such prediction models for various age groups of adolescents in the greater South Africa. / MSc (Sport Science), North-West University, Potchefstroom Campus, 2014