Os médicos veterinários que trabalham com répteis frequentemente são indagados pelos proprietários sobre os tipos de doenças que estes animais podem transmitir, bem como sobre as medidas profiláticas que devem ser implementadas para prevenir a transmissão de doenças. Desta forma, o conhecimento sobre os patógenos que estes animais albergam passa a ser importante para orientação dos proprietários quanto aos cuidados adequados que devem ser adotados com estes animais. Os microrganismos que compõem a microbiota podem se tornar patogênicos para seus hospedeiros quando os mesmos encontram-se debilitados, bem como a eliminação contínua destes microrganismos (pelas fezes, por exemplo) por répteis aparentemente saudáveis ou mesmo doentes, pode representar um importante problema para pessoas que tenham contato com eles. Crianças, idosos e indivíduos imunossuprimidos ou imunocomprometidos são bastante suscetíveis às infecções após manipulação de répteis criados como pet. Considerando-se que os répteis participam de forma crescente do mercado de animais criados como pet, suas características microbiológicas devem ser pesquisadas, visando evitar que eles adoeçam ou venham a óbito devido à ocorrência de doenças infecciosas e não transmitam zoonoses para aqueles que os manipulam. Este trabalho teve como objetivos o estudo da microbiota bacteriana e fúngica presente na cloaca de jabutis (Geochelone carbonária) criados em domicílio e análise do potencial risco a saúde humana. Foram realizados exames microbiológicos de swabs de cloaca de 100 jabutis-piranga visando a pesquisa de bactérias aeróbias, anaeróbias facultativas e fungos filamentosos e leveduras. Foram isolados 18 gêneros de bactérias, 06 gêneros de leveduras e 03 gêneros de fungos filamentosos. Os gêneros de microrganismos isolados com maior freqüência foram: Escherichia sp. (67%), Klebsiella spp. (54%), Bacillus spp. (42%), Candida spp. (42%), Citrobacter spp. (33%), Staphylococcus spp. (29%), Corynebacterium spp. (15%) e Aeromonas spp. (15%), dentre outros com menor freqüência. A freqüência de isolamentos de E. coli (67%) foi semelhante à de Klebsiella spp. (54%) e maior (P<0,05) do que as frequências de isolamentos de todos os outros microrganismos. Todos os microrganismos isolados podem representar risco para a saúde humana, devendo-se atentar para os cuidados com répteis criados como animais pet, particularmente quanto aos aspectos de higiene relacionados aos mesmos, visando assim a prevenção destes riscos. / Veterinarians who work with reptiles are frequently asked by owners about diseases that these animals can transmit, as well about the preventative measures that should be taken to prevent the transmission of diseases. Therefore, knowledge about pathogens that inhabit these animals and represent a potential zoonotic risk are very important in order to allow veterinarians to give proper recommendations about husbandry and preventative methods. Children, elderly people, and imunocompromised individuals are increasingly susceptible to infections after reptile pet manipulation. The microorganisms which form the microbiota can become pathogenic for their host if the host is debilitated. Transmission of these microorganisms from healthy or sick reptiles can be hazardous for people that have contact with them. Considering that reptiles are increasing obtained as pet, their microbiota should be investigated in order to prevent transmission of disease to people who manipulate them. The purposes of this paper were to evaluate the bacterial and fungal microbiota in the companion tortoise (Geochelone carbonaria) and to analyze the potential public health risk. One hundred samples of feaces from cloaca were obtained from jabutis-piranga and microbiological examinations were made for the presence of aerobic and facultative anaerobic bacteria, filamentous fungi and yeasts. Eighteen genus of bacteria, six genus of yeasts, and three genus of fungi were identified. The most frequently isolated genus were: Escherichia sp. (67%), Klebsiella spp. (54%), Bacillus spp. (42%), Candida spp. (42%), Citrobacter spp. (33%), Staphylococcus spp. (29%), Corynebacterium spp. (15%) and Aeromonas spp. (15%). The number of isolated E. coli (67%) was similar to that of Klebsiella sp. (54%), and was greater than (P<0.05) the total number of other species isolated. All isolated microorganisms present a public health risk. Therefore, care should be taken when obtaining these reptiles as pets, especially with regards to husbandry and proper hygiene in order to prevent the risk of contamination with the microbiota.

The present Thesis explored spatial reorientation behaviour of three species of fish (the zebrafish Danio rerio, the redtail splitfin fish Xenotoca eiseni, the goldfish Carassius auratus) and one species of reptiles (the Hermann tortoise Testudo hermanni) to widely assess three issues: 1) the use of environmental geometry with and without landmarks; 2) the role of two geometric tasks, one driven by spontaneous behaviour (“social-cued memory task”) and the other by learning processes (“rewarded exit task”); 3) the involvement of extra-visual sensory channels in visual transparency conditions, and motion patterns. The present Thesis applied behavioural assessments and analyses to pursue a line of comparison, across species, methodologies, and sensory systems. As regards environmental geometry and landmarks in fish and tortoises (Chapter 2), the studies were carried out within several apparatuses, that is, a rectangular opaque arena or two different sized square opaque arenas or a transparent square arena, with conspicuous or local landmarks: Study 1, Conspicuous landmark (blue wall) in zebrafish; Study 2: Local landmarks (corner panels) in zebrafish; Study 3, Environmental geometry in tortoises; Study 4, Conspicuous landmark (blue wall) in tortoises. As regards spontaneous vs. acquired geometric spatial reorientation in fishes (Chapter 3), the studies were carried out within a rectangular or square transparent arena, with or without geometric cues or a 3D landmark: Study 5, Nonvisual environmental geometry in zebrafish, redtail splitfin fish, and goldfish; Study 6, Isolated environmental geometric cues in zebrafish; Study 7, 3D outside landmark (blue cylinder) in zebrafish. As regards extra-visual sensory systems and motion patterns in fish (Chapter 4), one study was carried out within a rectangular transparent arena: Study 8, Lateral line pharmacological ablation in zebrafish. In respect of comparisons among species, overall results suggested that zebrafish, redtail splitfin fish, and goldfish reoriented similarly through transparent surfaces, which defined a distinctive global shape, supporting spatial reorientation under undefined situations (e.g., seek out food within a visually lacking and unenriched environment) as a shared skill among teleosts, despite ecological specificities. Likewise, the Hermann tortoise reoriented within a geometric environment with precision to meet a survival need, suggesting that even non-nomadic species that hibernate for long can benefit from orientation by extended terrain surfaces. In respect of memory tests (“working” vs. “reference”, spontaneous vs. acquired), overall results indicated that the rewarded exit task designed to train fish and tortoise to reorient required learning processes allowing them to overcome natural predispositions to improve other related abilities, such as landmark-use. The dissociation between working and reference memory in spatial domain must be considered highly dependent on task’s demands where attentional factors determine short-term memories and motivational states long-term ones. In respect of sensory channels and motion patterns, overall results revealed that fish and tortoises used modalities driven by touch, in synch with sight, to determine geometric parameters during spatial reorientation. Therefore, a promising link between other vertebrates and humans takes place, in consideration of orientation mechanisms used to face situations of visual deprivation or impairments. The present Thesis may even contribute to a general understanding of reorientation behaviour in phylogenetically remote vertebrate species, thus supporting the widespread use of geometry-grounded tools in everyday activities. This also provides comparative support among species that inhabit on Earth and share cognitive adaptations to deal with similar requests.

In this thesis we studied individuals of 4 species of tortoises, often bred in captivity genus Testudo species T. hermanni, T. graeca, T. horsfieldi and T. marginata, in possession of the ZOO Hluboká nad Vltavou. We studied and analyzed their morphological parameters, size measurements, their weight and biometrical indexes, wheather they follow described sexual dimorphism as it was found out in non-captive populations. We also studied and analyzed their food preferences to find out differences between sexes and between keeping individual animal versus a group, by giving them a choice between 4 types of food (the red cabbage, leaves of dandelion and clover, and carrot roots). Sexual dimorphism displayed by different body proportions was most apparent in Testudo hermanni. In specimen from the ZOO of Testudo horsfieldi a T. graeca was sexual dimorphism less prominent. Measured Testudo females from the ZOO were in lowest, approximate and highest values bigger and heavier than males of the same species, which mirrors data from other studies of non-captive populations. Males are quicker to get to and quicker to start eating food than females. Males preffered red cabbage and carrot while the females clover and dandelion. The amplitude of preference change between individual and group experiment was not correlated by individual´s sex, species or size. We found out no apparent hierarchical enforcement from the side of faster or bigger specimen in the feeding process.

The illegal reptile trade quandary in the Western Cape province is strategically and chronologically addressed in this thesis with the implicit intention of revealing its gamut and underlying dynamics, developing a pragmatic, parsimonious and authentic conservation crime category with clearly delineated parameters, and formulating an integrated theoretical explanation regarding its aetiology that will adequately explicate herpetological, and hopefully also other forms of natural resource, crime and deviance. The thesis, by essentially transcending traditional, stereotypical edicts, throws new light on a severely neglected and underestimated form of natural resource exploitation, highlighting the need for reptiles, as the sentinels of the state of our environmental health to be preserved and perpetuated for, in the final analysis, the benefit of human kind. Through an essentially explorative enquiry, utilising an integrated qualitative -quantitative research approach, the concept of conservation crime, as a vanguard to an innovative and unified conservation criminology, is introduced in this thesis in the form of unambiguous adjunct of the mainstream criminological discipline. It is, furthermore, utilised as a conduit within the herpetological crime framework to enrich the criminological discipline as a whole, broaden its frontiers, promote effective and focussed intervention/mitigation initiatives, as well as stimulate interest for further investigation in this field. Fragmented, antiquated and nebulous legislation, deficient conservation and related role-player organisational capacity and inconsistent penalties, in concert with apathetic (and decidedly generic) societal attitudes and traditional pessimistic rubric regarding reptiles, emerge as fundamental proclivities impeding the effective intercession and management of the natural resources embodied in this sphere. Injudicious manipulation of the Western Cape's scarce and specialised reptile resources and the biodiversity ramifications such exploitation realises portend the intensification and diversification potential of such criminality. Conservation criminology, as developed and presented in this thesis, underscores the significant contribution this field of criminology can make in comprehending the illegal manipulation/exploitation of herpetological and other natural resources, expanding and enhancing its theoretical constructs and implementing justice through decisive, dedicated and holistic intervention programmes/strategies in order to defend the inherent right to the continued existence of all reptile species. / Crimonology / D. Litt et Phil. (Criminology)

The illegal reptile trade quandary in the Western Cape province is strategically and chronologically addressed in this thesis with the implicit intention of revealing its gamut and underlying dynamics, developing a pragmatic, parsimonious and authentic conservation crime category with clearly delineated parameters, and formulating an integrated theoretical explanation regarding its aetiology that will adequately explicate herpetological, and hopefully also other forms of natural resource, crime and deviance. The thesis, by essentially transcending traditional, stereotypical edicts, throws new light on a severely neglected and underestimated form of natural resource exploitation, highlighting the need for reptiles, as the sentinels of the state of our environmental health to be preserved and perpetuated for, in the final analysis, the benefit of human kind. Through an essentially explorative enquiry, utilising an integrated qualitative -quantitative research approach, the concept of conservation crime, as a vanguard to an innovative and unified conservation criminology, is introduced in this thesis in the form of unambiguous adjunct of the mainstream criminological discipline. It is, furthermore, utilised as a conduit within the herpetological crime framework to enrich the criminological discipline as a whole, broaden its frontiers, promote effective and focussed intervention/mitigation initiatives, as well as stimulate interest for further investigation in this field. Fragmented, antiquated and nebulous legislation, deficient conservation and related role-player organisational capacity and inconsistent penalties, in concert with apathetic (and decidedly generic) societal attitudes and traditional pessimistic rubric regarding reptiles, emerge as fundamental proclivities impeding the effective intercession and management of the natural resources embodied in this sphere. Injudicious manipulation of the Western Cape's scarce and specialised reptile resources and the biodiversity ramifications such exploitation realises portend the intensification and diversification potential of such criminality. Conservation criminology, as developed and presented in this thesis, underscores the significant contribution this field of criminology can make in comprehending the illegal manipulation/exploitation of herpetological and other natural resources, expanding and enhancing its theoretical constructs and implementing justice through decisive, dedicated and holistic intervention programmes/strategies in order to defend the inherent right to the continued existence of all reptile species. / Crimonology / D. Litt et Phil. (Criminology)