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The structure and function of yeast k1 toxin /Zhu, Hong, 1963- January 1990 (has links)
No description available.
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A model system to study the effects of methylglyoxal on the yield and quality of tissue plasminogen activator produced by CHO cellsTriplett, Charla K. 07 September 1999 (has links)
In this research, a model system for studying the effects of the toxic metabolite, methylglyoxal, was created using Chinese Hamster Ovary (CHO) cells which produce tissue plasminogen activator (t-PA). The human gene for glyoxalase I was subcloned into an inducible mammalian expression vector. This vector was then used to create three stable CHO integrants, two control and one putative glyoxalase I producing cell lines. The CHO clones were characterized for the production of glyoxalase I using both SDS-PAGE gels and glyoxalase activity assays. In addition, the cell lines were evaluated to determine the levels of free methylglyoxal produced. The putative glyoxalase producer showed higher levels of glyoxalase I activity than the parent cell line and produced a unique protein band at the correct molecular weight. They also had a significantly lower level of free methylglyoxal than either of the two control cell lines. These cells can now be used as a tool to determine the specific effect of methylglyoxal on the yield and quality of tissue plasminogen activator produced. / Graduation date: 2000
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Characterization of structural, immunological, and biological properties of staphylococcal exfoliative toxin A (ETA) /Rago, James Vincent. January 1999 (has links)
Thesis (Ph.D.)--University of Minnesota, 1999. / Includes bibliographical references (leaves 98-112). Also available on the World Wide Web as a PDF file.
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Elucidation of ganglioside binding domain in the B-subunit of cholera toxinTan, T. F. January 2000 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves.
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Pharmacology of three classes of conopeptides that disrupt sympathetic neurotransmission /Sharpe, Iain A. January 2001 (has links) (PDF)
Thesis (Ph. D.)--University of Queensland, 2001. / Includes bibliographical references.
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Structure-based design and characterization of ricin and shiga toxin inhibitors /Miller, Darcie Jeanette, January 2001 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2001. / Vita. Includes bibliographical references (leaves 76-87). Available also in a digital version from Dissertation Abstracts.
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Elucidation of ganglioside binding domain in the B-subunit of cholera toxin檀東煇, Tan, T. F. January 2000 (has links)
published_or_final_version / Biochemistry / Master / Master of Philosophy
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Biological activity of purified staphylococcal alpha toxinO'Dea, Kathleen Karen January 1979 (has links)
No description available.
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Methods for the production, measurement, and determination of immunospecificity of toxin Z by several strains of Pseudomonas aeruginosaWong, Francis Sze-Ho, 1949- January 1976 (has links)
No description available.
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The structure and function of yeast k1 toxin /Zhu, Hong, 1963- January 1990 (has links)
The carboxyl-terminal sequences of the $ alpha$ and $ beta$ subunits of the secreted yeast K1 toxin have been determined by protein sequencing and amino acid analysis of peptide fragments generated from the purified toxin. It revealed that the $ alpha$ and $ beta$ subunits consist of amino acid residues 45-147 and 236-316 from the preprotoxin, respectively. The preprotoxin configuration can be represented as: prepropeptide-ArgPro-$ alpha$-ArgArg-$ gamma$-LysArg-$ beta$. This structure for the preprotoxin defines a specific processing pathway in yeast involving a dibasic endoprotease, encoded by the KEX2 gene and a carboxypeptidase B like enzyme which is probably encoded by the KEX1 gene. / By using the patch-clamp technique, it is shown both in vivo with sensitive yeast spheroplasts and in vitro with asolectin liposomes that the toxin forms ion permeable channels. The toxin induced ion channels are voltage independent with a unit conductance of 118pS, often appearing in pairs and prefer monovalent cations. / K1 toxin has a much wider killing spectrum at the spheroplast level than at the whole cell level as demonstrated by the fact that the toxin kills spheroplasts from the genera Candida, Kluyveromyces, and Schwanniomyces, whose cells are toxin insensitive. A toxin binding study shows that the wall receptor can define toxin specificity and is necessary but not sufficient for toxin action on intact cells. / Using various mutagenesis techniques, a set of mutations throughout regions encoding the $ alpha$ and $ beta$ subunits that allow secretion of mutant toxins were generated. By analyzing the phenotypes of these mutant toxins, the ion channel forming domain is assigned exclusively to the hydrophobic $ alpha$ subunit and the cell wall receptor binding domain is localized to both the $ alpha$ and $ beta$ subunits.
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