Made available in DSpace on 2016-04-28T20:18:27Z (GMT). No. of bitstreams: 1 2006-Daniel da Silva Guedes Junior.pdf: 6474539 bytes, checksum: feffc8f41781fb68d79aa3969d5d9651 (MD5) Previous issue date: 2006-02-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Babesiosis, anaplasmosis, trypanosomosis and borreliosis are relevant diseases, potentially causing morbidity in cattle, leading to economic losses. The objective of this study was to determine, through indirect enzyme-linked immunosorbent assay (ELISA), the prevalence of Babesia bigemina, Babesia bovis, Anaplasma marginale, Trypanosoma vivax and Borrelia sp. antibodies in cattle to estabilish the risk of outbreaks. Sera samples of 246 female adult cattle from Castanhal and S?o Miguel do Guam?, northeastern Par? counties (areas with recorded copper and cobalt deficiency) were used. Crude antigen was used in the serologic technique to detect antibodies against B. bigemina, B. bovis, T. vivax and Borrelia sp. For the detection of A. marginale antibodies, recombinant major surface 1a protein (MSP1a) was used as antigen for indirect ELISA. The serum samples showed a prevalence of 99.2% (B. bigemina), 98.8% (B. bovis), 68.3% (A. marginale), 93.1% (T. vivax) and 54.9% (Borrelia sp). The municipalities were considered stable enzootic areas for B. bigemina, B. bovis and T. vivax, but unstable ones for A. marginale and Borrelia sp. In spite of the stability determined for some studied areas, babesiosis, anaplasmosis and trypanosomosis cases with severe clinical signs were seen on the studied region, possibly due to the mineral deficiency that decreases the immune response and immunoreativity of the animals. / A babesiose e a anaplasmose juntamente com a tripanossomose e borreliose s?o doen?as de import?ncia por causarem, potencialmente, morbidade no rebanho bovino, causando preju?zos ? economia. O presente trabalho teve como objetivo investigar, atrav?s do teste ELISA indireto, a preval?ncia de anticorpos para Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax e Borrelia burgdorferi em bovinos, avaliando o risco de surtos de doen?a. Foram utilizadas amostras de soro de 246 bovinos f?meas, adultas, provenientes dos munic?pios de Castanhal e S?o Miguel do Guam?, regi?o nordeste do estado do Par? com registros de defici?ncias de Cobre e Cobalto. Foi feita sorologia atrav?s do ensaio imunoenzim?tico ELISA indireto, utilizando-se ant?genos brutos para os agentes: B. bigemina, B. bovis, T. vivax e B. burgdorferi. Em rela??o a A. marginale, foi utilizada a prote?na recombinante MSP1a como ant?geno. A an?lise dos soros revelou a preval?ncia para B. bigemina de 99,2%, B. bovis 98,8 %, A. marginale 68,3%, T. vivax 93,1% e Borrelia sp 54,9%. Os munic?pios estudados foram caracterizados como enzooticamente est?veis para B. bigemina, B. bovis e T. vivax, e enzooticamente inst?veis para A. marginale e Borrelia sp. Apesar da estabilidade prevista para alguns agentes na ?rea do estudo, casos cl?nicos de babesiose, anaplasmose e tripanossomose ocorrem na regi?o, possivelmente devido ? defici?ncia mineral, que leva a diminui??o da resposta imune e da imunorreatividade org?nica dos animais.