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LD2668 .T4 1962 N66

Typescript (photocopy). / Digitized by Kansas Correctional Industries

Call number: LD2668 .T4 1979 S38 / Master of Science

Typescript (photocopy). / Digitized by Kansas Correctional Industries

Hierdie studie het ten doel gehad om die effekte van Se en β-karoteentoediening op vit A, vit E, konsepsietempo en progesteroonvlakke tydens embrio-oorplasings in ontvangerkoeie te ondersoek. Die effekte van die verskillende faktore op mekaar sou ook bepaal word. In Totaal van 142 multipare koeie wat in vier groepe verdeel is, was as ontvangers vir die studie gebruik. Die groepe was as volg behandel: Groep 1 (35 koeie), 50 mg Se en 1500 mg vit E intramuskulêr 6 en 3 weke voor die oorplasings; Groep 2 (36 koeie), 300 mg l1-karoteen per os per bees per dag vanaf 6 weke voor die oorlasings; Groep 3 (36 koeie), vit E/Se en β-karoteen soos in Groepe 1 en 2 respektiewelik; Groep 4 (35 koeie) negatiewe kontrole groep met geen behandeling nie. Elke ontvanger het 6 weke voor oorplasing vit ADE oraal ontvang. Embrios is na 20, 19, 19 en 18 ontvangers van Groepe 1, 2, 3 en 4 onderskeidelik oorgeplaas. Bloedmonsters is van elke dier vir ontledings in die middel van die oorplantingsperiode getrek. Die toediening van Se was hoogs betekenisvol met RBS GSH-Px aktiwiteit gekorreleer R2 = 0,32960, (n = 136, P = 0,0000). Die toediening van β-karoteen was ook hoogs betekenisvol gekorreleer met die RBS GSH-Px aktiwiteit, R2 = 0,18950, (n=136, P< 0,0000). Die RBS GSH-Px aktiwiteit was 7,49,6,61,10,75 en 4,5 EE 11x1010 RBS vir Groepe 1, 2, 3 en 4 onderskeidelik. Groepe 1 en 3,1 en 4, 2 en 3, 2 en 4, en 3 en 4 het betekenisvol van mekaar verskil maar nie Groepe 1 en 2 nie. Daar was geen verband tussen β-karoteen plasmavlakke en RBS GSH¬Px aktiwiteit nie. Die orale toediening van β-karoteen het plasmavlakke duidelik laat styg en was betekenisvol met die β-karoteen plasmavlakke gekorreleer R2 = 0,09296, (n = 134, P=O,0003). Die groepe wat β-karoteen ontvang het, Groepe 2 en 3, se plasmavlakke was merkbaar hoër as die van Groepe 1 en 4 wat niks ontvang het nie. Die groep se waardes was 8,72, 10,13, 9,53 en 8,50 µmol/l vir Groepe 1, 2, 3 en 4 respektiewelik. Groepe 1 en 2, 2 en 4, en 3 en 4 het betekenisvol van mekaar verskil (P=0,0021) maar nie Groepe 1 en 3, en 1 en 4 nie. Daar was 'n positiewe korrelasie tussen die toediening van vit E/Se en die a¬tocoferol plasmavlakke, R2 = 0,06344, (n = 134, P=0,0033). Daar was egter geen aanduiding van 'n verband tussen RBS GSH-Px aktiwiteit en α-tocoferol plasmavlakke nie. Daar was wel In betekenisvolle korrelasie tussen α-tocoferol en β-karoteen plasmavlakke (P=0,0001). Die groepswaardes was 11,90, 10,59, 11,67 en 10,56 vir Groepe 1, 2, 3 en 4 onderskeidelik. Groepe 1 en 2, en 1 en 4 het betekenisvol van mekaar verskil (P=0,0334) maar nie die ander groepe, Groepe 1 en 3, 2 en 3, 2 en 4, en 3 en 4 nie. Die korrelasie tussen die toediening van Se en die retinol plasmavlakke was nie statisties betekenisvol nie (P=0,0671) maar tussen RBS GSH-Px aktiwiteit en retinol plasmavlakke was dit wel betekenisvol R2 = 0,03086, (n = 133, P=0,0431). Daar was ook In waarskynlike korrelasie tussen die toediening van β-karoteen en die retinol plasmavlak (P=0,0835) maar geen korrelasie tussen β-karoteen plasmavlakke en retinol plasmavlakke nie. Die groepwaardes was 1,068, 1,065, 1,154 en 1,026 µmol/l vir Groepe 1, 2, 3 en 4 onderskeidelik. Die dragtigheidstempo na die oorplasing van embrios was 50,0%, 47,4%, 31,5% en 27,8% vir Groepe 1, 2, 3 en 4 onderskeidelik. Die verskille was nie betekenisvol nie. Daar was 'n negatiewe korrelasie tussen die β-karoteen plasmavlakke en progesteroon plasmavlakke vir die eerste 5-9 dae van die estrussiklus. Retinol plasmavlakke en progesteroon plasmavlakke was wel positief gekorreleer (P=O,0317). Die groepswaardes was 5,67, 2,90, 4,28 en 5,60 µmol/l vir Groepe 1, 2, 3 en 4 onderskeidelik. RBS GSH-Px was betekenisvol gekorreleer met corpus luteum grootte maar corpus luteum grootte was nie met progesteroon waardes gekorreleer nie. Daar was geen statistiese verskille tussen die groepe ten opsigte van estruslengte en die tydsduur tussen prostaglandientoediening en estrus nie. Op individuele basis was daar wel In betekenisvolle korrelasie van β-karoteen plasmavlak met beide estruslengte (P=O,0298) en die tydsduur tussen prostaglandientoediening en estrus (P=0,0130). Estruslengte en die tydsduur tussen prostaglandien en estrus was ook betekenisvol met mekaar gekorreleer (P < 0,0000). / This study addressed some of the effects that selenium and β-carotene administration have on vit A, vit E, conception rates, progesterone levels, oestrus length, interval from PG to oestrus and corpus luteum size in embryo recipients. A total of 142 multiparous cows asigned to 4 test groups, were used as recipients in this study. Group 1 (35 cows) received 50 mg Se and 1500 mg vit E intramuscularly 6 and 3 weeks before transfer, Group 2 (36 cows) received 300 mg β-carotene orally per cow per day starting 6 weeks prior to transfer, Group 3 (36 cows) received both vit ElSe and β-carotene as per Group 1 and 2 and Group 4 (35 cows) was a negative control, with no treatment. All recipients received vit ADE orally 6 weeks before transfers. Embryos were transferred to 20, 19, 19, and 18 recipients of the Groups 1, 2, 3 and 4 respectively. Blood for analysis was drawn from all the recipients in the middle of the transferal week, being day 3 of the 5-day period. The administration of selenium was highly significantly correlated with the corresponding RBS GSH-Px activity R2 = 0,3290, (n = 135, P < 0,0000). The β-¬carotene administration was also highly significantly correlated with the RBS GSH¬Px activities R2 = 0,18950, (n = 136, P < 0,0000). However, there were no correlation between the β-carotene plasma levels and that of the RBS GSH-Px activities. The RBS GSH-Px were 7,49, 6,61, 10,75 and 4,5 Enzyme Units 11x1010 RBS for Groups 1, 2, 3 and 4 respectively. Groups 1 and 3, 1 and 4, 2 and 3, 2 and 4, and 3 and 4 differ significantly from each other but not Groups 1 and 2. There were no connection between B-carotene plasma levels and RBS GSH-Px activities. The treatment of β-carotene itself was succesful and was significantly correlated with the resulting β-carotene plasma levels R2 = 0,09296, (n = 134, P = 0,0033). The β-carotene plasma levels of the groups that received β-carotene, Groups 2 and 3, were noticeably higher than those of Group 1 and 4, which did not receive β-carotene. The values were 8,72, 10,13, 9,53 and 8,5 µmol/l for Groups 1, 2, 3 and 4 respectively. Groups 1 and 2, 2 and 4, and 3 and 4 differed significantly from one another (P = 0,0021) but not those of Groups 1 and 3, and 1 and 4. There was a positive correlation between the administration of Se and the retinol plasma levels (P = 0,0671) and a positive significant correlation between the RBS GSH-Px and the retinol plasma levels R2 = 0,03086, (n = 133, P = 0,0431). There was also a positive correlation between the administration of β-carotene and the retinol plasma levels (P = 0,0835) but no correlation between β-carotene plasma levels and retinol plasma levels. The values were 1,068, 1,065, 1,154 and 1,026 µmol/ for Groups 1, 2, 3 and 4 respectively. The pregnancy rate after embryo transferal were 50%, 47,4%, 31,5% and 27,8% for Groups 1, 2, 3 and 4 respectively. The differences were not significant. There was a negative correlation between β-carotene plasma levels and progesterone plasma levels for the first 5 to 9 days of the oestrus cycle. Retinol plasma levels however were significantly correlated with progesterone plasma levels (P = 0,0317). The progesterone levels were 5,67, 2,90, 4,28 and 5,60 µmol/ for Groups 1, 2, 3 and 4 respectively. The RBS GSH-Px activity was significantly correlated with corpus luteum size (P = 0,0445) but corpus size was not correlated with progesterone values. There were no statistical differences amongst the groups, between oestrus length and the interval from prostaglandin to oestrus. However on an individual basis there was a significant correlation between β-carotene plasma levels and both the oestrus length (P = 0,0298) and the interval from prostaglandin to oestrus (P = 0,0130). Oestrus length and the interval from prostaglandin to oestrus were also significantly correlated with each other (P < 0,0000). / Dissertation (MMedVet)--University of Pretoria, 2000. / Production Animal Studies / MMedVet / Unrestricted

Die vorliegende Arbeit verfolgte das Ziel, neben dem biographisch-persönlichen Werdegang von Johannes RICHTER auch die essentiellen Aspekte seiner fachlichen Arbeit auf dem Gebiet der Tierzucht und insbesondere der Tiergeburtshilfe zu beleuchten. Dies resultiert aus der Tatsache, dass bis zum heutigen Zeitpunkt noch keine genaue Betrachtung der Entwicklung des Fachgebietes in Dresden bzw. Leipzig vorliegt. Als Quellenbasis dienten für die Dissertation neben einer beachtlichen Anzahl an Archivalien - insbesondere aus dem Sächsischen Hauptstaatsarchiv in Dresden sowie dem Leipziger Universitätsarchiv - Berichte aus zeitgenössischer Literatur. Daneben konnte auch Material aus der Hand von RICHTERs Nachkommen verwendet werden. In einigen Bereichen musste die Verfasserin auf Sekundärliteratur zurückgreifen. Im ersten Abschnitt erfolgt die Darstellung des Lebenslaufes von Johannes RICHTER. Seiner Familie, privaten Interessen, seinem gesundheitlichen Zustand und auch RICHTERs politischer Einstellung wird dabei besondere Beachtung geschenkt. Der zweite Teil dieser Dissertation widmet sich den zahlreichen Arbeiten RICHTERs in seiner Funktion als Leiter der Ambulatorischen, ab 1911 auch der Geburtshilflichen Klinik in Dresden. Da diese Arbeiten Fortsetzung in der Errichtung des Institutes für Tierzucht und Geburtskunde finden, das 1923 nach Leipzig übernommen wurde, wird der Einrichtung hier besondere Beachtung geschenkt. Die Probleme des Institutes infolge der Wirtschaftskrise und später durch den Ausbruch des 2. Weltkrieges stellen - wie auch die Nachfolge RICHTERs nach 1943 - ebenfalls zentrale Themen des zweiten Abschnitts dar. Johannes RICHTER, der von 1906 bis 1912 Direktor der Ambulatorischen Tierklinik war, gab 1912 nach dem Tod des Tierzüchters PUSCH dieses Direktorat ab, erhielt die Professur für Tierzucht und stand damit bis zu seinem Tod 1943 dem Institut für Tierzucht und Geburtskunde vor. Während dieser Zeit gab es kaum ein Teilgebiet der Tiergeburtshilfe, Gynäkologie und Andrologie bei Rindern, Pferden, Schweinen, Schafen und Kleintieren, auf dem er nicht tätig war. Seine wissenschaftlichen Veröffentlichungen umfassen die Geburtshilfe im engeren Sinne mit all ihren Zweigen: die Geburt, das Puerperium und deren Störungen, die Therapie von Genitalerkrankungen, Jungtiererkrankungen, Ursachen und Bekämpfung der Sterilität und schließlich tierzüchterische Fragen der Fortpflanzung und Besamung. Neben seiner unermüdlichen Arbeit an der Dresdener und später Leipziger Bildungsstätte in Lehre und Forschung war Johannes RICHTER Mitglied zahlreicher Verbände und Gesellschaften – darunter die Kaiserlich Deutsche Akademie der Naturforscher zu Halle. Anerkennung fand seine Tätigkeit auch in zahlreichen Ehrungen: So erhielt er im Dezember 1915 das Eiserne Kreuz II. Klasse sowie am 7. März 1916 in Lüttich das Ritterkreuz I. Klasse mit Schwertern des Sächsischen Albrechtsordens. 1918 wurden ihm Titel und Rang eines Medizinalrates verliehen. In der anschließenden Diskussion werden die essentiellen Aspekte nochmals umrissen und unter Hinzuziehung von eigenen Erkenntnissen vertieft. Das Hauptaugenmerk liegt dabei auf der Einstellung RICHTERs zum Nationalsozialismus, die im Zusammenhang zu seinem Suizid - einem weiteren Schwerpunkt - zu sehen ist. Die Autorin kommt hier zu dem Ergebnis, dass RICHTER lediglich eine deutschnationale Einstellung vertrat, sich aber nie politisch engagierte. RICHTER war kein Mitglied der NSDAP und bekannte sich auch nur dann öffentlich zum Nationalsozialismus, wenn er dazu verpflichtet war bzw. wenn ein Zuwiderhandeln mit persönlichen Nachteilen verbunden gewesen wäre. RICHTERs Bedeutung für die Weiterentwicklung der Tiergeburtshilfe wird ebenfalls reflektiert. Dabei galt es insbesondere zu klären, welchen Wert seine Fachliteratur heutzutage hat. Hervorzuheben ist in diesem Zusammenhang RICHTERs „Lehrbuch der Tiergeburtshilfe“ als Neuauflage von „HARMS´ Lehrbuch der tierärztlichen Geburtshilfe“, das bis heute als Standardwerk gilt. Auf dem Gebiet der Veterinärmedizin hat Johannes RICHTER so bemerkenswerte Spuren für die Nachwelt hinterlassen.

Orientador: Cezinande de Meira / Coorientador: José Nicolau Próspero Puoli Filho / Banca: Nereu Carlos Prestes / Banca: Cláudia Barbosa Fernandes / Resumo: Pouco se sabe sobre o perfil de progesterona (P4) sintetizada pelos corpos lúteos suplementares (CLS) e de que forma tais estruturas se relacionam com a gestação em receptoras gestantes acíclicas tratadas com progestágeno. Sendo assim, os principais objetivos deste estudo foram avaliar o efeito do tratamento com altrenogest durante 70 ou 120 dias de gestação sobre a manutenção da gestação em receptoras acíclicas e correlacionar os achados com o número de CLS, tempo de formação e concentração de P4 encontrada no momento da interrupção do tratamento, visando melhorar a compreensão da fisiologia da gestação e avaliar a possibilidade de redução do tempo da administração de progestágenos em receptoras acíclicas gestantes. Foram avaliadas 25 éguas, gestantes por transferência de embrião (TE) na fase de anestro, transição e durante a estação de monta, distribuídas em diferentes agrupamentos, de acordo com a variável estudada: tempo de tratamento hormonal com altrenogest (grupos 70ALT, 120ALT e Controle), período de formação dos CLS e perfil de P4 (grupos Anestro, Transição e Controle). Os animais foram avaliados por meio de ultrassonografia e quantificação de P4 plasmática até os 130 dias de gestação. Após interrupção hormonal aos 70 dias no grupo 70ALT, observou-se manutenção de cinco gestações e dois abortamentos. No grupo 120ALT não ocorreu abortamento após a interrupção do tratamento aos 120 dias de gestação. O período médio (dias) da primeira formação dos CLS foi similar (p=0,32) nos grupos 70ALT e 120ALT e maior (p<0,01) que o encontrado no Controle. As médias da primeira formação dos CLS foram diferentes (p<0,01) entre os grupos Anestro, Transição e Controle. Nas éguas acíclicas a síntese de P4 ocorreu em período mais avançado... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: There are few reports about progesterone (P4) synthesis by supplementary corpora lutea (SCL) and how these structures interfere with gestation in noncyclic pregnant recipient mares treated with progestagens. Therefore, the main objectives of this study were to evaluate the effect of altrenogest treatment during 70 or 120 days of gestation on pregnancy maintenance in non-cyclic recipient mares and to correlate these findings with SCL number, formation period and P4 concentration at the moment of treatment interruption, in order to improve the understanding of pregnancy physiology and to evaluate the possibility of progestagens treatment reduction in non-cyclic recipient mares. Twenty five pregnant mares by embryo transfer (ET) in anestrous, transitional and ovulatory phase were evaluated and distributed into different groups, according to the studied variable: altrenogest treatment period (70ALT, 120ALT and Control groups), SCL formation period and P4 profile (Anestrous, Transition and Control groups). Mares were evaluated by ultrassonography and quantification of plasma progesterone until 130 days of gestation. After hormonal withdrawal on group 70ALT, five pregnancy maintenance and two abortions were observed at 75 days of gestation. No abortion was noted on group 120ALT after treatment interruption. The mean period (days) of first SCL formation was similar (p=0,32) in 70ALT and 120ALT groups and greater (p<0.01) than Control group. Means of the first SCL formation were different (p<0.01) among Anestrous, Transition and Control groups. Progesterone synthesis in non-cyclic mares occurred in more advanced gestational period and showed lower concentration during the 130 days in relation to cyclic mares. It is suggested that progestagen treatment interruption in... (Complete abstract click electronic access below) / Mestre

Made available in DSpace on 2014-06-11T19:29:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-27Bitstream added on 2014-06-13T20:19:12Z : No. of bitstreams: 1 silva_esm_me_botfmvz.pdf: 1141700 bytes, checksum: 6038621ff1d9adc1012053624dd32bb6 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Pouco se sabe sobre o perfil de progesterona (P4) sintetizada pelos corpos lúteos suplementares (CLS) e de que forma tais estruturas se relacionam com a gestação em receptoras gestantes acíclicas tratadas com progestágeno. Sendo assim, os principais objetivos deste estudo foram avaliar o efeito do tratamento com altrenogest durante 70 ou 120 dias de gestação sobre a manutenção da gestação em receptoras acíclicas e correlacionar os achados com o número de CLS, tempo de formação e concentração de P4 encontrada no momento da interrupção do tratamento, visando melhorar a compreensão da fisiologia da gestação e avaliar a possibilidade de redução do tempo da administração de progestágenos em receptoras acíclicas gestantes. Foram avaliadas 25 éguas, gestantes por transferência de embrião (TE) na fase de anestro, transição e durante a estação de monta, distribuídas em diferentes agrupamentos, de acordo com a variável estudada: tempo de tratamento hormonal com altrenogest (grupos 70ALT, 120ALT e Controle), período de formação dos CLS e perfil de P4 (grupos Anestro, Transição e Controle). Os animais foram avaliados por meio de ultrassonografia e quantificação de P4 plasmática até os 130 dias de gestação. Após interrupção hormonal aos 70 dias no grupo 70ALT, observou-se manutenção de cinco gestações e dois abortamentos. No grupo 120ALT não ocorreu abortamento após a interrupção do tratamento aos 120 dias de gestação. O período médio (dias) da primeira formação dos CLS foi similar (p=0,32) nos grupos 70ALT e 120ALT e maior (p<0,01) que o encontrado no Controle. As médias da primeira formação dos CLS foram diferentes (p<0,01) entre os grupos Anestro, Transição e Controle. Nas éguas acíclicas a síntese de P4 ocorreu em período mais avançado... / There are few reports about progesterone (P4) synthesis by supplementary corpora lutea (SCL) and how these structures interfere with gestation in noncyclic pregnant recipient mares treated with progestagens. Therefore, the main objectives of this study were to evaluate the effect of altrenogest treatment during 70 or 120 days of gestation on pregnancy maintenance in non-cyclic recipient mares and to correlate these findings with SCL number, formation period and P4 concentration at the moment of treatment interruption, in order to improve the understanding of pregnancy physiology and to evaluate the possibility of progestagens treatment reduction in non-cyclic recipient mares. Twenty five pregnant mares by embryo transfer (ET) in anestrous, transitional and ovulatory phase were evaluated and distributed into different groups, according to the studied variable: altrenogest treatment period (70ALT, 120ALT and Control groups), SCL formation period and P4 profile (Anestrous, Transition and Control groups). Mares were evaluated by ultrassonography and quantification of plasma progesterone until 130 days of gestation. After hormonal withdrawal on group 70ALT, five pregnancy maintenance and two abortions were observed at 75 days of gestation. No abortion was noted on group 120ALT after treatment interruption. The mean period (days) of first SCL formation was similar (p=0,32) in 70ALT and 120ALT groups and greater (p<0.01) than Control group. Means of the first SCL formation were different (p<0.01) among Anestrous, Transition and Control groups. Progesterone synthesis in non-cyclic mares occurred in more advanced gestational period and showed lower concentration during the 130 days in relation to cyclic mares. It is suggested that progestagen treatment interruption in... (Complete abstract click electronic access below)

The present studies were conducted to gain a better understanding of the effects of pregnenolone (P₅), human chorionic gonadotropin (hCG) and 3' 5', cyclic adenosine monophosphate ( cAMP) on porcine placental and endometrial production of progesterone (P₄), testosterone (T) and estrone (E₁) at 30, 60 and 90 days of gestation. Duplicate 300 mg samples of placenta, endometrium or both (co-incubation) were incubated in medium199 containing either no P₅, P₅, P₅ + hCG or P₅ + cAMP for either zero (control), .5, 1 or 2 h. The first study compared P₄ and E₁ production with or without addition of P₅. At d 30, 60 and 90, respectively, P₄ production (ng/g) increased significantly in the presence (vs absence) of P₅ in the incubation medium of placental (13.2 vs 7.5, 73.9 vs 42.7, 137.4 vs 113.5, respectively) coincubation (14.5 vs 10.0, 33.6 vs 22.3, 77.9 vs 49.4, respectively) and endometrial (16.0 vs 13.3, 23.0 vs 16.0, 17.1 vs 6.7, respectively) tissue. Presence of P₅ increased E₁ production in d 60 (1.3 vs .7 ng/g) and d 90 (51.7 vs 34.6 ng/g) placental tissue and d 90 endometrial tissue (9.8 vs 8.0 ng/g). In a second study, P₅ + cAMP increased (vs P₅ alone) P₄ in placental tissue at d 30 (11.6 vs 8.7 ng/g) and coincubation tissue at d 90 (103.7 vs 75.3 ng/g). Cyclic AMP stimulated increased P₄ synthesis ( vs P₅ alone), throughout the incubation period in d 60 and d 90 tissue. E₁ production by endometrial tissue at d 30 (4.1 vs 2.9 ng/g), and placental tissue at d 60 ( 1. 2 vs . 9 ng/g). Presence of hCG in the incubation medium had no overall effect on either P₄ or E₁ accumulation. Only trace amounts of T were detected in either study, suggesting rapid aromatization of C₁₉ steroids to estrogens. / M.S.