Thesis (PhD)-- Stellenbosch University, 2014. / ENGLISH ABSTRACT: This thesis explores the relationship between African traditional livestock regimes and state veterinary services in colonial Zimbabwe from the perspective of socio-environmental history. It offers a new direction both methodologically and empirically as few academic studies have used state veterinary services archives extensively as a lens to understanding the parameters of the interaction of veterinarians and African livestock owners during the colonial period. Though located in socio-environmental history, this study has applicability to the histories of medicine, conservation and land policy as it connects with the broader debate regarding the experiences of local healing practices under colonial administrations. It examines the complex, fluid and interactive interdependence of people, livestock and disease, and discusses how veterinary medicine, conservation policies, and introduced epizootics impacted on African traditional livestock regimes. It demonstrates how African livestock owners reacted to veterinary challenges, and how they understood veterinary and environmental arguments mobilized by the colonial state to justify segregation. It shows that state veterinary services were not limited to pharmacological drugs and the administration of inoculants but also extended to breeding and other livestock improvement activities such as pasture management. It argues that the provision of state veterinary services was largely influenced by the shifting, contradictory relationship involving the state, native commissioners and white settlers. Given the fractured nature of colonial administration in Southern Rhodesia, this thesis also discusses conflicts between colonial experts (veterinary and animal scientists) and African livestock owners over what type of cattle to rear, how they were to be pastured, and also how epizootics and enzootics could be eradicated or controlled. Key Words: conservation; African livestock regimes; veterinary medicine, local healing practices; dipping; therapeutics; acaricides; centralisation; socio-environmental history; liberation war; Zimbabwe; Southern Rhodesia; Rhodesia. / AFRIKAANSE OPSOMMING: This thesis explores the relationship between African traditional livestock regimes and state veterinary services in colonial Zimbabwe from the perspective of socio-environmental history. It offers a new direction both methodologically and empirically as few academic studies have used state veterinary services archives extensively as a lens to understanding the parameters of the interaction of veterinarians and African livestock owners during the colonial period. Though located in socio-environmental history, this study has applicability to the histories of medicine, conservation and land policy as it connects with the broader debate regarding the experiences of local healing practices under colonial administrations. It examines the complex, fluid and interactive interdependence of people, livestock and disease, and discusses how veterinary medicine, conservation policies, and introduced epizootics impacted on African traditional livestock regimes. It demonstrates how African livestock owners reacted to veterinary challenges, and how they understood veterinary and environmental arguments mobilized by the colonial state to justify segregation. It shows that state veterinary services were not limited to pharmacological drugs and the administration of inoculants but also extended to breeding and other livestock improvement activities such as pasture management. It argues that the provision of state veterinary services was largely influenced by the shifting, contradictory relationship involving the state, native commissioners and white settlers. Given the fractured nature of colonial administration in Southern Rhodesia, this thesis also discusses conflicts between colonial experts (veterinary and animal scientists) and African livestock owners over what type of cattle to rear, how they were to be pastured, and also how epizootics and enzootics could be eradicated or controlled. Key Words: conservation; African livestock regimes; veterinary medicine, local healing practices; dipping; therapeutics; acaricides; centralisation; socio-environmental history; liberation war; Zimbabwe; Southern Rhodesia; Rhodesia.

This Ph.D. dissertation examined the effects of bacterial DNA on immune surveillance in ovine lymph nodes (LN). The first study identified long-term changes in lymph nodes following exposure to plasmid DNA. Treated lymph nodes were heavier and had larger medullary areas. Furthermore, medullary cord thickness and medullary sinus width as well as germinal center size and number were increased in plasmid treated lymph nodes.<p>To determine whether bacterial DNA altered cell trafficking through lymph nodes, the efferent lymphatic of the prescapular LN of sheep was cannulated and cells were collected. Intradermal injection of as little as 4 ìg of plasmid DNA expressing the green fluorescent protein of jellyfish (eGFP) caused a marked increase in the cell trafficking through the prescapular lymph node. A dose-dependent facet existed for this response, as the increase in cell trafficking response persisted longer with 40 ìg or 400 ìg of plasmid-eGFP than with 4 ìg. This increased cell trafficking was independent of green fluorescent protein expression as both pCAN1-eGFP and pCAN1 induced similar responses.<p>Increased cell traffic induced by bacterial DNA was further characterized by determining whether bacterial DNA form was critical for this response. Treatment with intact plasmid (circular) DNA induced the greatest increase in cell traffic. In contrast, when plasmid DNA was digested with restriction enzymes into linear fragments of DNA, then cell trafficking was not significantly increased. <p>Numerous studies have shown that immunostimulatory guanosine-cytosine (CpG) motifs activate cells in vitro and can improve protective immune responses in vivo . Therefore we tested the hypothesis that the presence of immunostimulatory CpG motifs within plasmids was responsible for altering cell trafficking. It was shown that plasmids with added immunostimulatory CpG sequences affected cell trafficking in a dose-dependent manner. The injection of 40 ìg of pBISIA-88 caused an increase in cell trafficking while injecting 400 ìg pBISIA-88 failed to increase cell flow above control levels.<p>Analysis of cell populations collected in efferent lymph gives insight into treatment effects on immune surveillance. This is well-studied following treatment with antigen and in general, antigen induces a selective movement of cells into lymph that occurs 24 hours post-treatment. In contrast, plasmid DNA induced a rapid, non-selective movement of lymphocytes through the lymph node, suggesting that bacterial DNA and antigen may affect cell trafficking by different mechanisms and thereby have distinct effects on immune surveillance.<p>Short-term effects of bacterial DNA on lymph node architecture and cellular composition were also investigated to determine whether an increase in cell trafficking was associated with structural changes within the lymph node. While it was shown that bacterial DNA induced changes within a lymph node, including and increased number of germinal centers and an increased frequency of CD72*CD21* B cells, these changes were not correlated to increased cell trafficking.<p>In summary, bacterial DNA altered immune surveillance in sheep lymph nodes by changing cell traffic and lymph node architecture and composition. Many of these responses differed from responses observed by others following antigen treatment. The present observations suggest that bacterial DNA can function as an important signal to modulate immune surveillance and host responses to infections by pathogens.

This Ph.D. dissertation examined the effects of bacterial DNA on immune surveillance in ovine lymph nodes (LN). The first study identified long-term changes in lymph nodes following exposure to plasmid DNA. Treated lymph nodes were heavier and had larger medullary areas. Furthermore, medullary cord thickness and medullary sinus width as well as germinal center size and number were increased in plasmid treated lymph nodes.<p>To determine whether bacterial DNA altered cell trafficking through lymph nodes, the efferent lymphatic of the prescapular LN of sheep was cannulated and cells were collected. Intradermal injection of as little as 4 ìg of plasmid DNA expressing the green fluorescent protein of jellyfish (eGFP) caused a marked increase in the cell trafficking through the prescapular lymph node. A dose-dependent facet existed for this response, as the increase in cell trafficking response persisted longer with 40 ìg or 400 ìg of plasmid-eGFP than with 4 ìg. This increased cell trafficking was independent of green fluorescent protein expression as both pCAN1-eGFP and pCAN1 induced similar responses.<p>Increased cell traffic induced by bacterial DNA was further characterized by determining whether bacterial DNA form was critical for this response. Treatment with intact plasmid (circular) DNA induced the greatest increase in cell traffic. In contrast, when plasmid DNA was digested with restriction enzymes into linear fragments of DNA, then cell trafficking was not significantly increased. <p>Numerous studies have shown that immunostimulatory guanosine-cytosine (CpG) motifs activate cells in vitro and can improve protective immune responses in vivo . Therefore we tested the hypothesis that the presence of immunostimulatory CpG motifs within plasmids was responsible for altering cell trafficking. It was shown that plasmids with added immunostimulatory CpG sequences affected cell trafficking in a dose-dependent manner. The injection of 40 ìg of pBISIA-88 caused an increase in cell trafficking while injecting 400 ìg pBISIA-88 failed to increase cell flow above control levels.<p>Analysis of cell populations collected in efferent lymph gives insight into treatment effects on immune surveillance. This is well-studied following treatment with antigen and in general, antigen induces a selective movement of cells into lymph that occurs 24 hours post-treatment. In contrast, plasmid DNA induced a rapid, non-selective movement of lymphocytes through the lymph node, suggesting that bacterial DNA and antigen may affect cell trafficking by different mechanisms and thereby have distinct effects on immune surveillance.<p>Short-term effects of bacterial DNA on lymph node architecture and cellular composition were also investigated to determine whether an increase in cell trafficking was associated with structural changes within the lymph node. While it was shown that bacterial DNA induced changes within a lymph node, including and increased number of germinal centers and an increased frequency of CD72*CD21* B cells, these changes were not correlated to increased cell trafficking.<p>In summary, bacterial DNA altered immune surveillance in sheep lymph nodes by changing cell traffic and lymph node architecture and composition. Many of these responses differed from responses observed by others following antigen treatment. The present observations suggest that bacterial DNA can function as an important signal to modulate immune surveillance and host responses to infections by pathogens.

The purpose of this study was to analyse personnel expenditure of the Directorate Veterinary Service in the North West Province and how it impacted on service delivery. Literature on personnel expenditure and its influence on service delivery, particularly in the field of Veterinary Services is still in its infancy. Financial condition was referred as a tool to evaluate trends and patterns in how personnel budgets increases over a period of time as a fraction of the total budget affected the ability of the directorate to deliver services to the citizens of the province. Financial condition uses a percentage change to analyse changes in the budget over time for the Directorate. Budget reports (2000-2002) collected from the Provincial Department of Agriculture, Conservation and Environment was analyzed in terms of a split between personnel and operational. This analysis was then used to elucidate patterns over time for the Directorate as a whole, which were then compared between the regions (using cost centers) to determine any bias in terms of resource allocation. The percentage change was also done per standard budget item used in the Province. From the study it is evident that there was deterioration in financial condition for the financial year 200112001. This deterioration in financial condition is shown by a drop in budget but also in terms of a bias towards personnel. This phenomenon was however reversed in the following year of 2002/2003. This study has identified a negative correlation between personnel budget and other expenditure,:, needs for the Directorate Veterinary Services that had negative consequences for service delivery. To this extent it recommends a regular monitoring process to be put in place and the use of public partnerships where practical as a solution to rising personnel costs.

The purpose of this study was to analyse personnel expenditure of the Directorate Veterinary Service in the North West Province and how it impacted on service delivery. Literature on personnel expenditure and its influence on service delivery, particularly in the field of Veterinary Services is still in its infancy. Financial condition was referred as a tool to evaluate trends and patterns in how personnel budgets increases over a period of time as a fraction of the total budget affected the ability of the directorate to deliver services to the citizens of the province. Financial condition uses a percentage change to analyse changes in the budget over time for the Directorate. Budget reports (2000-2002) collected from the Provincial Department of Agriculture, Conservation and Environment was analysed in terms of a split between personnel and operational. This analysis was then used to elucidate patterns over time for the Directorate as a whole, which were then compared between the regions (using cost centers) to determine any bias in terms of resource allocation. The percentage change was also done per standard budget item used in the Province. From the study it is evident that there was deterioration in financial condition for the financial year 200112001. This deterioration in financial condition is shown by a drop in budget but also in terms of a bias towards personnel. This phenomenon was however reversed in the following year of 2002/2003. This study has identified a negative correlation between personnel budget and other expenditure needs for the Directorate Veterinary Services that had negative consequences for service delivery. To this extent it recommends a regular monitoring process to be put in place and the use of public partnerships where practical as a solution to rising personnel costs. / (M.B.A.) North-West University, Mafikeng Campus, 2004

Thesis (M.P.A.)--Kutztown University of Pennsylvania, 1997. / Source: Masters Abstracts International, Volume: 45-06, page: 2934. Abstract precedes thesis as preliminary leaves 2-3. Typescript. Includes bibliographical references (leaves 39-47).

Please read the abstract in the 00front part of this document / Dissertation (MA (Social Work))--University of Pretoria, 2006. / Social Work / unrestricted

There is currently no independent monitoring and evaluation structure for state agricultural or veterinary services to support the “Batho Pele” principle of effective and efficient service delivery to the people of South Africa. Participatory rural appraisal was used in Moretele District, North West Province, to design, implement and assess veterinary extension on the tick and worm parasites of cattle. Veterinary extension, in contrast to agricultural extension, is defined as practical and understandable advice given to individual, groups, communities and population about livestock diseases and includes their prevention, treatment and control, as well as the way they influence the well being, health, and productivity of both humans and animals. The study was conducted in Moretele, which lies about 60km north of Pretoria, and is divided into three service delivery wards, each managed by an animal health technician. A random sample of 30 beef cattle farmers, each with a minimum of 10 cattle, was done in each ward. From each of these 90 farmers, five indicator cattle were purposively selected to include two calves, two sub-adults and one adult animal. Adult feeding ticks were sampled from predetermined sites and eggs per gram were estimated from pooled faecal samples of the same animals. The knowledge levels of animal health technicians (N=44) were assessed prior to the extension being given to the farmers and it was found to be inadequate. They were then given further training. Demographics and knowledge level of farmers were assessed using structured interviews. Baseline sampling for parasites was done on the indicator cattle. A farmer’s day and monthly extension using the visit and training method of extension was done with the farmers over a period of 12 months. The level of knowledge of the farmers was reassessed and the indicator cattle resampled at the same time of the year as the first sampling. It was found that although there was a significant increase in the farmers knowledge, there were no significant differences in the level or species of parasites. It was concluded that animal health technicians did not normally have sufficient knowledge of the subject to give farmers affective extension messages. It was also concluded that knowledge and implementation of extension are not the same thing and that further research is required into the reasons for lack of implementation. / Dissertation (MSc (Veterinary Sciences))--University of Pretoria, 2004. / Paraclinical Sciences / unrestricted

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