Antibiotics are widely used pharmaceuticals in human and veterinary medicine. These compounds are biologically active. They decrease efficiency of biological processes in wastewater treatment plants. Antibiotics are not eliminated from sewage water completely and they are discharged as contaminants into the receiving waters. Several methods exist for the determination of antibiotics in sewage water. In the first place liquid chromatography, gas chromatography and capillary electrophoresis are used. The aim of this thesis was to developed a suitable izolation technique and an optimal analytical method for identification and determination of penicillins in wastewater. For determination was selected amoxicillin, ampicillin and benzylpenicillin (penicillin G). These are very often used penicillins. An optimized method was used for determination of these penicilins in real samples from a wastewater treatment plant situated in Veterinary and pharmaceutical university in Brno and from the large-scale wastewater treatment plant in Brno-Modřice. Real samples were concentrated using SPE (solid phase extraction). For penicillins determination were used high performance liquid chromatography with diode array detection (HPLC/DAD).

According to valid legislation inorganic substance determination will be analyzed in the sludges withdrawn from the Modrice sewage disposal plant. Based on gained results subsumption of the sludge into categories will be carried out and method of their use will be suggested.

Large polycyclic aromatic hydrocarbons with molecular mass exceeding 278 Da are rarely object of study, in spite of the fact that some compounds from this group are proved carcinogens. These compounds belong to the group of persistent organic compounds and they are considered as dangerous environmental contaminants. Diploma thesis is focused on the evaluation of the occurence of large PAHs in technological and waste waters produced in black pitch processing in the DEZA Valašské Meziříčí factory. The method of their analysis based on reversed-phase liquid chromatography with non-aqueous gradient; UV-VIS and fluorescence detection will be used. This method will enable the study of changes of large PAHs distribution at various stages of waste water treatment process.

The theses compares analytical methods according to current norms used in the Czech Republic to the methods based on the principle according to US EPA. A new device the automatic photometer Aquakem 250 makes use of the US EPA methods. Prior to its laboratory use in the Institute of Public Health in the town of Ostrava the photometer had to be validated. For the comparsion of the analytical methods ion chromatographic, isotachophoresis, spectrophotometric,titrimetric methods were used. The validation process, which was documented (in validation protocols), was time consuming and technically challenging. It was confirmed that the new Aquakem 250 analyzer provides result that are verified by methods which are in accordance with norms applicable in the Czech Republic. After a successful completion of validation and obtained accreditation the new automatic analyzer was put into regular operation in the chemically laboratory.

In the text of the master´s thesis, it is at first briefly referred about Energetic Material Measurement topic in general. Emphasis is placed especially at the description of the Velocity of Detonation and short analysis of selected measurement method. The most significant part of the paper is dedicated to the design and description of the system that was created in ISE Design Suite environment using VHDL language. The development was performed with respect to oncoming integration into the board with FPGA and A/D converters. The operation of detection algorithm which was created based on the MATLAB model was verified in the final part of the thesis by simulation of processing of real optical probe signals.

The aim of the thesis is to Mutěnice wastewater treatment plant, which lies in the wine region of Moravia sub-slovácko propose technological measures to the Autumnal wine campaign harboring extreme overload wastewater treatment plant Mutěnice. It will establish a number of options which will be compared with each other.

Karst rivers are special type of rivers because of their hydrography conditions. They usually flow from non-karst area and as soon as they reach in to the karst area they disappear underground. There, they flow through cavities until they reach the surface again. The aim of this thesis is to assess the impact of water outflow from wastewater treatment plant on karst rivers. The study was done in the central area of CHKO Moravský kras where the impact of two WWTP on a cave system Rudické propadání – Býčí skála is evaluated. As a part of this study a half-year monitoring was carried out, which consisted of water sampling once every two weeks. In total, 13 water samples were taken from 8 sampling points. Also 4 additional one-time samples were taken from the cave system. Moreover, flow rate, temperature, conductivity, oxygen concentration and pH were measured at the sampling point. Afterwards, the content of undissolved solids, CODCr, BOD, N-NH4+, N-NO2-, N-NO3-, P-PO43- and total phosphorus were determined in the samples. The change of parameters in time and during the flow in the river was observed from the measured values. The balance of pollutants in particular parts of the river was calculated. The outflow values from waste water treatment plants were compared to the emission limits for surface waters and the parameters of rivers were compared to the environmental quality standards. According to the results, it can be observed that the water quality improves after passing through the cavities. It was proven that the outflows from the observed waste water treatment plants are negatively influencing the water streams. The most serious problem is the wash up of sludge from WWTP Rudice and its consequent sedimentation in the river and/or outflow to the Rudické propadání.

The target of the first part of this diploma thesis is focused on the problems relating to the sludge production from the wastewater treatment plant and subsequent sludge treatment. In this thesis complete process of the wastewater treatment is described. Predominantingly is described the process in digestion tanks, therefore stabilization process, whereas the paper is focused on sludge pre-treatment (disintegration) of waste activated sludge before stabilization. The second part of this thesis is focused on the design of the disintegration unit for the wastewater treatment sludge in assist with experimental data. According to measured and fixed parameters the unfired pressure vessel has been designed. On the vessel has been further realized the strength calculation, the heat balance and the economic balance.

is Diploma’s thesis is addressing the issues of technical drying, principles and types of technical drying, solar drying possible usefulness of sewage sludge in the Czech Republic, its availability during the season, the legislation related to the processing of sewage sludge and design of pilot scale solar dryer.

The aim of this thesis is to assess the impact of water outflow from wastewater treatment plant on karst rivers. The study was done in the northern area of Moravský kras protected landscape, where the impact of WWTP Ostrov u Macochy on a cave system Lopačské propadání was studied. As a part of this study a half-year monitoring was carried out, which consisted of water sampling every two weeks. In total, 13 water samples were taken from 7 sampling points. Moreover, flow rate, temperature, conductivity, oxygen concentration and pH were measured at the sampling point. Afterwards, the content of undissolved solids, COD, BOD, N-NH4+, N-NO2-, N-NO3-, NKi and total phosphorus were determined in the samples. The change of parameters in time on the sampling points and further along the flow of the river was evaluated from the measured values. The outflow values from waste water treatment plants were compared with the emission limits for surface waters and the values of river water parameters were compared with the environmental quality standards. It wasn’t proven that the outflow from the observed waste water treatment plant is negatively influencing the water stream. The most serious problems are: -The WWTP was designed for treatment of sewage from Ostrov u Macochy, which involved separate sanitary and storm sewers. After finishing the WWTP, a uniform sewer was built. -High concentration of total phosphorus on the outflow from WWTP. -Poor quality of Lopač brook water already before the outflow vent from WWTP.