Weakly electric fish use of a self-generated electric field to probe their environment, this behaviour is known as electrolocation. This study investigated two aspects of electrolocation in two species of knifefish (Apteronotus leptorhynchus and Eigenmannia virescens). First, we characterized the ability to track moving objects and found that tracking performance did not differ among speeds tested in either species. Second, we characterized a motion-related cue for distance perception, similar to visual parallax, for which rapidly moving objects would be perceived as closer than slowly moving objects. During tracking experiments, the fish remained centered between the moving objects. We hypothesized that the fish use electrosensory parallax to perform this centering behaviour. Thus, we predicted that if one object moved slightly slower than the other, the fish would perceive the slower-moving object as farther away, and would move towards the slower object to remain “centered.” Indeed, our results supported our hypothesis with E. virescens moving towards the slower object to an extent that increased with the relative decrease in speed.

Effective exploration of the environment is a critical aspect of adaptive behaviour, enabling animals to identify food sources, potential mates, refuge locations, and other important resources. The particular strategies used during exploratory behaviours depend on a variety of factors including context, personality traits and natural ecology. Weakly electric fish rely specifically on a short-range electric sense to search and locate objects in their environment in low-light conditions. However, little is known about the exploratory strategies used. We characterized the exploratory movements of two species of weakly electric fish, Apteronotus leptorhynchus and Apteronotus albifrons, in a laboratory setting. Our results suggest that there are behavioural differences between species in their exploratory strategies. Apteronotus albifrons spent more time in the open, travelled at a slower speed when out in the open, and had a higher total feeding time. Interestingly, Apteronotus leptorhynchus had a higher total displacement and preference for wall-following. A subsequent study on the behavioural function of wall-following in the two species suggested that wall-following is used for exploration in weakly electric fish, rather than for protection, and is not an artifact of restricted movement and tank shape.

Weakly electric fish use of a self-generated electric field to probe their environment, this behaviour is known as electrolocation. This study investigated two aspects of electrolocation in two species of knifefish (Apteronotus leptorhynchus and Eigenmannia virescens). First, we characterized the ability to track moving objects and found that tracking performance did not differ among speeds tested in either species. Second, we characterized a motion-related cue for distance perception, similar to visual parallax, for which rapidly moving objects would be perceived as closer than slowly moving objects. During tracking experiments, the fish remained centered between the moving objects. We hypothesized that the fish use electrosensory parallax to perform this centering behaviour. Thus, we predicted that if one object moved slightly slower than the other, the fish would perceive the slower-moving object as farther away, and would move towards the slower object to remain “centered.” Indeed, our results supported our hypothesis with E. virescens moving towards the slower object to an extent that increased with the relative decrease in speed.

Eigenmannia virescens is a gymnotiform weakly electric fish which uses a quasi-sinusoidal electric organ discharge (EOD) to sense their environment. EOD frequency (EODF) is individual-specific. In conspecific interactions, each fish perceives the EODF of the conspecific as a periodic amplitude modulation (AM) of their own discharge. When both fish are stationary, the depth of this AM is constant, but it varies when fish are swimming. We hypothesized that AM variations during swimming act as a noise source that could have no effect on, hinder, or enhance EODF identification. To test this, we quantified the jamming avoidance response (JAR) (a natural behaviour wherein fish are required to accurately determine one another’s EODF) in response to stimuli of varying depths of noise. These experiments demonstrated that swimming noise does not impair the ability of E. virescens to identify conspecific EODF, and actually improves its ability to detect the presence of a neighbouring fish.

The dorsodorsal division (DD) of the teleost telencephalon has been implicated in memory processes similar to those associated with the mammalian hippocampus. The network connectivity and neural activity underlying this involvement have remained unclear. This thesis attempts to elucidate both. Attempts have been made to record the neural activity of DD neurons, but none have succeeded in correlating the recorded firing with any meaningful stimuli. In this thesis, I present single-unit electrophysiological recordings of DD neurons that reveal persistent activity in the form of up-states which are evoked by two modalities of naturalistic sensory stimuli – visual and electrosensory. The anatomy of DD was a little better understood than the neural activity. Recent anatomical work has shown that DD is strongly inter-connected with the cortical-like dorsolateral division (DL) of the pallium, re-inforcing its similarity to mammalian hippocampal structures. This same work has also revealed much of DD’s extrinsic connectivity. It was not, however, of a resolution fine enough to disambiguate the connections of the various DD subregions, nor to clarify the existence and structure of intrinsic DD connectivity. In this thesis, I further elucidate the connectivity of DD, by isolating its subregions. This was done by means of very small and precise neurotracer injections. These injections revealed strong recurrent connectivity within individual DD subregions, multiple pathways between DD and DL, and striking similarities between the connectivities of DL and DD and those of the mammalian dentate gyrus and CA3 respectively. From the results of these investigations I propose a model of homology between the teleost DD-DL loop and the putative pattern separation and completion networks contained in the mammalian cortico-hippocampal circuitry, as well as a role for the observed persistent activity in DD within this model. I further propose the dorsal teleost telencephalon as an excellent model system for the further study of the network mechanisms of pattern separation and completion.

The weakly electric fish Sternopygus macrurus emits an electric organ discharge (EOD) composed of a series of pulses. The EOD pulse is mainly shaped by sodium currents. There are two sodium channel α subunits orthologs of the mammalian Nav1.4 expressed in the EO of Sternopygus. Previous studies identified a novel splice variant of the Nav1.4b (Nav1.4bL), in which an extra 51-amino acid occurs in the N terminal end. Nav1.4bL currents inactivate and recover from inactivation significantly faster than Nav1.4bS. The voltage-dependence of steady-state inactivation of smNav1.4bL shifts to hyperpolarized potential. Structural analysis predicts an α-helix in the middle of the extended N terminus. Removal of a proline right after the α-helix significantly slows down current decay but has no effect on channel recovery from inactivation, suggesting inactivation and recovery have independent mechanism. Mutagenesis analysis of the extended N terminus showed that the short helical region, especially the positive charges in the helix, is an important determinant for channel voltage-dependence of steady-state inactivation. However, other residues outside the helical region are required for regulation of fast inactivation and recovery form inactivation. Functional and structural analysis provides evidence for the importance of the C terminus in fish Nav1.4b channel properties. Chimera in which the C terminus of smNav1.4bS was substituted by the human Nav1.4 C terminus, shows an 11 mV positive shift in voltage-dependence of activation and a -16 mV negative shift in inactivation. Deletion of the distal half of smNav1.4bS negatively shifted voltage-dependence of inactivation and significantly accelerated channel recovery from inactivation. In the deletion mutant, the regulation by the N segment is missing. Substitution of the C terminus mutant retains wild type channel inactivation and recovery properties and can be regulated by N segment again. My study provides evidence that the extended N terminus of smNav1.4bL binds the distal part of C terminal tail to modulate channel inactivation properties. This is the first time to show the distal C terminus is involved in channel recovery from inactivation. Studies in the fish sodium channel properties provide useful information to understand function and structure of voltage-gated sodium channels. / text

Behavioural syndromes, defined as suites of correlated behaviours across different contexts, are used to characterize individual variability in behaviours. Males of the weakly electric fish species, Apteronotus leptorhynchus, produce electro-communication signals called chirps. Chirps are thought to be involved in agonistic signalling, as their relative incidence increases during agonistic conspecific interactions. However, high levels of individual variability in aggression obscure the role of chirps in mediating aggression. Here, I tested the presence of an aggression-boldness behavioural syndrome, and then considered the implications such a syndrome would have on chirping behaviours. Behavioural tests in anti-predation, object novelty, feeding, conspecific intrusion and novel environment exploration contexts revealed a syndrome involving only object novelty and feeding. We found no correlation between chirping behaviour and the assessed behaviours. Our results demonstrate that chirps represent a more complex communication system than previously suggested.

Behavioural syndromes, defined as suites of correlated behaviours across different contexts, are used to characterize individual variability in behaviours. Males of the weakly electric fish species, Apteronotus leptorhynchus, produce electro-communication signals called chirps. Chirps are thought to be involved in agonistic signalling, as their relative incidence increases during agonistic conspecific interactions. However, high levels of individual variability in aggression obscure the role of chirps in mediating aggression. Here, I tested the presence of an aggression-boldness behavioural syndrome, and then considered the implications such a syndrome would have on chirping behaviours. Behavioural tests in anti-predation, object novelty, feeding, conspecific intrusion and novel environment exploration contexts revealed a syndrome involving only object novelty and feeding. We found no correlation between chirping behaviour and the assessed behaviours. Our results demonstrate that chirps represent a more complex communication system than previously suggested.

The goal of this thesis is to identify differences and consistencies in the trajectories taken by foraging agents before and after they have learned the location of a target. The challenge is that these agents do not go directly towards the target after learning and keep a certain amount of randomness in their paths. We use different versions of discrete curvature and head angle as tools in this analysis. We also build models of foraging agents using stochastic processes with data supported parameters.

Construímos um aparato experimental para medir os instantes de disparo do órgão elétrico de peixes elétricos de campo fraco da espécie Gymnotus carapo, que produz estes pulsos para localizar objetos dentro da água e para se comunicar socialmente. O aparato foi desenvolvido de maneira a iisolar o animal de perturbações externas como vibrações mecânicas, sons, campos elétricos e variações de luminosidade do ambiente. A principal característica de nosso aparato é um conjunto de eletrodos, distribuídos nos vértices do tanque de experimentos, que permitem obter as medidas (longas séries de instantes de disparo) sem restringir os movimentos do peixe e até mesmo inferir a sua posição comparando as amplitudes em diferentes eletrodos, o que possibilita relacionar a posteriori os padrões de disparo ao comportamento do animal. Desenvolvemos um programa de computador em linguagem C que, através de uma interface digital­analógica reproduz a série temporal da voltagem de um pulso de um peixe verdadeiro e utilizamos este sinal elétrico para estimular os animais. Os pulsos artificiais foram aplicados a um dipolo elétrico que imita a geometria do órgão elétrico de um peixe e os intervalos entre pulsos foram produzidos por diferentes distribuições: aleatória, intervalos gravados previamente do próprio ou de outro peixe, sequências manipuladas para repetir determinados trechos reais intercalados com trechos aleatórios, etc. Um segundo computador foi utilizado para detectar os instantes dos pulsos de estímulo e resposta e armazenar estas sequências em arquivos. Posteriormente utilizamos estas sequências para calcular a informação mútua média entre os sinais e verificamos que diferentes peixes reconhecem e reagem (alterando seus disparos elétricos) a determinados trechos da série de estímulo real de maneira bastante reprodutível. Também desenvolvemos outro programa de controle para detectar os pulsos do peixe em um dos aquários e estimular, em tempo real, o peixe de outro aquário e vice­versa. Assim, a única forma de interação entre os peixes é através dos pulsos elétricos e esta interação ocorre de modo bidirecional. Os dados destes experimentos também foram analisados utilizando o cálculo da informação mútua média entre os padrões dos dois peixes e encontramos evidências de que neste caso o fluxo de informação é maior que nos experimentos unidirecionais. Nosso aparato permitiu utilizar com sucesso a teoria da informação para estudar a dinâmica de disparo durante a interação elétrica entre peixes e possibilita diversos experimentos futuros em que pretendemos relacionar os padrões elétricos ao comportamento social dos animais e a sua interação com o meio ambiente. / We built an experimental apparatus to measure the electric organ discharge times from weakly electric fishes of the Gymnotus carapo species. Such fishes use these pulses to actively locate objects in water as well as in social communication. Our apparatus was designed to allow such measures in the absence of some external perturbations the fishes are sensitive to, such as mechanical vibrations, electric fields and changes in the laboratory luminosity. A set of eight electrods were installed in the corners of the experimental tank and allows to obtain the discharge times without need to restrain the movements of the fish. Actually, from the maximal amplitudes of the discharge in different elecrodes we can infer the position and movements of the fish and relate its electrical dynamics to its behavior. A computer program (C language) was written to use a digital to analog interface to reproduce the time series of a discharge pulse from a real fish (recorded previously) and this electrical signal was used to stimulate the animals. The artificial pulses were applied to an electrical dipole built to mimic the geometry of the electrical organ of a living fish. The intervals between discharges were chosen from sequences obtained from different distributions: random, sequencies from real living fishes, handled sequencies where we repeated some real patterns with random patterns in between, etc. The detection of the stimuli and response pulses were done in another computer with the software Dasylab and the discharge times sequencies were recorded in harddisk for further analysis. Both sequencies were used to compute the average mutual information between the signals and we verified that different fishes recognize and react (changing their pulse interval pattterns) to the same regions of the real stimuli sequence. We also developed another control program (C language) to detect the discharges of a fish in one tank and to stimulate, in real time, a fish in another tank with those pulses, and vice­versa, in a bidirectional way. In this way, the only interaction between the fishes is through their electric pulses. The data analysis also consisted in obtaining the average mutual information between the sequencies of the two fishes and we found evidences that the flow of information is higher than that found in unidirectional experiments. Our apparatus allowed us to succesfully apply information theory to study the dynamics of the discharge intervals when the fishes are interacting. In the future we intend to extensivelly use such experiments to relate the electrical patterns to social behavior and to the interaction of these fishes with their environment.