Thesis Erasmus University Rotterdam. / ook verschenen in gedrukte versie. With bibliogr., with a summary in Dutch.

Proefschrift Maastricht. / Met lit.opg. - Met samenvatting in het Nederlands.

Proefschrift Universiteit Maastricht. / Auteursnaam op omslag: Frank R.M. Stassen. Met bibliogr. van de auteur, lit. opg. - Met samenvatting in het Nederlands.

Drug dependence is a reality in South Africa, and adolescents are often admitted to treatment centres for rehabilitation. However, it seems that adolescents admitted to treatment centres sometime show resistance, which may lead to the underutilisation of the therapeutic programme. In addition, social workers in the drug dependence field do not have sufficient knowledge to handle the resistance shown by the adolescent drug dependant during therapeutic intervention. The researcher is not aware of any current strategies to identify and utilise resistance. The purpose of this study is to formulate a handling strategy for social workers, which will enable them to identify and utilise the resistance that adolescent drug dependants manifest during the therapeutic intervention in order to allow adolescents to make optimal use of their treatment programmes. To achieve this goal, the Design and Development Model of Rothman and Thomas were used in the intervention research. This study made use of qualitative research in order to work in an exploratory and descriptive way. During Phase 1, i.e. the problem analysis and project planning phase, data was collected through semi-structured interviews conducted with five social workers. These interviews were transcribed and analysed using Creswell’s data analysis spiral. Data has also been collected during Phase 2, which consists of data collection and synthesis by utilising existing sources of information, studying natural examples and identifying the functional elements of successful models. During Phase 3, which is the design phase, an observation system has been designed in the form of a questionnaire aimed at identifying resistance in adolescent drug dependants and at determining procedures for the intervention. A prototype handling strategy was development during Phase 4 (early design and development). This strategy may help social workers working in the field of drug dependence to identify and utilise resistance that appears in adolescent drug dependants during therapeutic intervention. / MSW, North-West University, Potchefstroom Campus, 2014

Resistant behaviour is a serious reality in South African primary schools. South Africa’s teachers strive to be agents of positive change amidst the multiple challenges they and their learners encounter, yet they admittedly experience daily frustration at addressing resistant behaviour. Considering the harsh external realities which many learners encounter, resistant behaviour cannot be expected to disappear at the introduction of specific techniques. The research consequently aimed to examine and describe Grade 5 teachers’ perceptions of resistance with the objective of improving teacher-learner relationships. A phenomenological paradigm structured this qualitative study to determine how grade 5 teachers perceive resistance through their direct experiences. The research involved focus group discussions and interviews. Fourteen teachers participated, representing three private and three public schools in Gauteng. Selection criteria included: a recognised teaching qualification, teaching experience of minimum two years, currently teaching Grade 5 learners, a commitment to teach demonstrated through course attendance and enhancement of the school’s curriculum, and the ability to speak English. During each focus group discussion, one introductory question was asked: “Please share your perceptions of resistant behaviour shown by children in your Grade 5 classes”. Additional funnelling and probing questions were utilized. Prior to data gathering, one participant per school was requested to volunteer to participate in an individual interview, in addition to the focus group discussion. During the interview, two introductory questions were asked: “How did you experience the focus group discussion pertaining to teacher perceptions of the resistant behaviour displayed by children in Grade 5?” and “What is your view of the conclusion/s reached during the discussion?” These questions were followed by funnelling questions. Content analysis was used with Interpretative phenomenological analysis to understand meanings ascribed to coded texts. Emerging findings were depicted visually to identify data patterns as part of thematic analysis until themes crystallised. Amongst findings understood from a causal, contextual and developmental perspective, unique findings emerged revealing that a relationship exists between teachers’ perceptions of resistant behaviour and the school ethos, that resistance can reveal creativity and divergent thinking processes, and that considering resistant behaviour from a future-minded perspective can enable teachers to see resistant behaviour as indicative of underlying skills needed by society, if developed as strengths. The findings support international research with one marked exception: that the examples provided in the literature affect the resistant learner personally, whereas the examples provided by the participants affect the other learners, teachers and the school itself. The findings contribute meaningfully to the debate regarding how to manage school resistance. The researcher recommends further studies be carried out to determine if the findings are reflective of most Grade 5 teachers. If so, it is advised that the findings be shared so that teachers become aware of alternate ways to interpret resistance and possibly to enhance their professional development by reformulating their current thought processes around resistance. The researcher recommends that the relationship between school ethos and resistant behaviour be explored to assist teachers in contextualising their management of resistance. / MA (Psychology), North-West University, Potchefstroom Campus, 2014

Drug dependence is a reality in South Africa, and adolescents are often admitted to treatment centres for rehabilitation. However, it seems that adolescents admitted to treatment centres sometime show resistance, which may lead to the underutilisation of the therapeutic programme. In addition, social workers in the drug dependence field do not have sufficient knowledge to handle the resistance shown by the adolescent drug dependant during therapeutic intervention. The researcher is not aware of any current strategies to identify and utilise resistance. The purpose of this study is to formulate a handling strategy for social workers, which will enable them to identify and utilise the resistance that adolescent drug dependants manifest during the therapeutic intervention in order to allow adolescents to make optimal use of their treatment programmes. To achieve this goal, the Design and Development Model of Rothman and Thomas were used in the intervention research. This study made use of qualitative research in order to work in an exploratory and descriptive way. During Phase 1, i.e. the problem analysis and project planning phase, data was collected through semi-structured interviews conducted with five social workers. These interviews were transcribed and analysed using Creswell’s data analysis spiral. Data has also been collected during Phase 2, which consists of data collection and synthesis by utilising existing sources of information, studying natural examples and identifying the functional elements of successful models. During Phase 3, which is the design phase, an observation system has been designed in the form of a questionnaire aimed at identifying resistance in adolescent drug dependants and at determining procedures for the intervention. A prototype handling strategy was development during Phase 4 (early design and development). This strategy may help social workers working in the field of drug dependence to identify and utilise resistance that appears in adolescent drug dependants during therapeutic intervention. / MSW, North-West University, Potchefstroom Campus, 2014

Resistant behaviour is a serious reality in South African primary schools. South Africa’s teachers strive to be agents of positive change amidst the multiple challenges they and their learners encounter, yet they admittedly experience daily frustration at addressing resistant behaviour. Considering the harsh external realities which many learners encounter, resistant behaviour cannot be expected to disappear at the introduction of specific techniques. The research consequently aimed to examine and describe Grade 5 teachers’ perceptions of resistance with the objective of improving teacher-learner relationships. A phenomenological paradigm structured this qualitative study to determine how grade 5 teachers perceive resistance through their direct experiences. The research involved focus group discussions and interviews. Fourteen teachers participated, representing three private and three public schools in Gauteng. Selection criteria included: a recognised teaching qualification, teaching experience of minimum two years, currently teaching Grade 5 learners, a commitment to teach demonstrated through course attendance and enhancement of the school’s curriculum, and the ability to speak English. During each focus group discussion, one introductory question was asked: “Please share your perceptions of resistant behaviour shown by children in your Grade 5 classes”. Additional funnelling and probing questions were utilized. Prior to data gathering, one participant per school was requested to volunteer to participate in an individual interview, in addition to the focus group discussion. During the interview, two introductory questions were asked: “How did you experience the focus group discussion pertaining to teacher perceptions of the resistant behaviour displayed by children in Grade 5?” and “What is your view of the conclusion/s reached during the discussion?” These questions were followed by funnelling questions. Content analysis was used with Interpretative phenomenological analysis to understand meanings ascribed to coded texts. Emerging findings were depicted visually to identify data patterns as part of thematic analysis until themes crystallised. Amongst findings understood from a causal, contextual and developmental perspective, unique findings emerged revealing that a relationship exists between teachers’ perceptions of resistant behaviour and the school ethos, that resistance can reveal creativity and divergent thinking processes, and that considering resistant behaviour from a future-minded perspective can enable teachers to see resistant behaviour as indicative of underlying skills needed by society, if developed as strengths. The findings support international research with one marked exception: that the examples provided in the literature affect the resistant learner personally, whereas the examples provided by the participants affect the other learners, teachers and the school itself. The findings contribute meaningfully to the debate regarding how to manage school resistance. The researcher recommends further studies be carried out to determine if the findings are reflective of most Grade 5 teachers. If so, it is advised that the findings be shared so that teachers become aware of alternate ways to interpret resistance and possibly to enhance their professional development by reformulating their current thought processes around resistance. The researcher recommends that the relationship between school ethos and resistant behaviour be explored to assist teachers in contextualising their management of resistance. / MA (Psychology), North-West University, Potchefstroom Campus, 2014

Genetically modified (GM) cotton expressing Cry1Ac proteins was released in South Africa in 1997 for control of the bollworm complex on this crop. No reports of the failure of Bollgard® cotton to control these pests have yet been made. Throughout the world there are concerns about the development of resistance of target pests to Bt cotton due to the use of only one Bt gene. The aim of this study was to determine if Helicoverpa armigera (Lepidoptera: Noctuidae) and Diparopsis castanea (Lepidoptera: Noctuidae) developed resistance to Bt cotton in South Africa. To determine if H. armigera developed resistance, laboratory experiments were conducted to determine the levels of larval survival and development time when feeding on Bt and non–Bt cotton. Bollworm populations were collected on maize and cotton at different sites in South Africa and reared on Bt and non–Bt cotton under laboratory conditions. Results showed that some populations survived on Bt cotton and that a significant proportion of the individuals successfully completed their life cycles on Bt cotton. Surveys were also conducted amongst cotton farmers to determine the levels of compliance to the refuge strategy that has to be implemented by farmers as an insect resistance management (IRM) strategy to delay resistance development. The levels of compliance to refugia requirements were low and farmers generally only started planting refugia several years after they planted Bt cotton for the first time. The development of resistance of H. armigera to Bt cotton in South Africa can possibly be ascribed to non–compliance to the prescribed refuge requirements. No conclusions can be made on resistance of D. castanea to Bt cotton but the relatively long time to mortality of larvae could indicate development of tolerance to Cry1Ac proteins. The new generation Bollgard II® cotton, expressing both Cry1Ac and Cry2Ab2 proteins, has been released in South Africa during the 2010/11 growing season and field observations showed effective control of the bollworm complex at several sites in the country. Monitoring of refuge compliance levels as well as resistance development in the bollworm complex to Bollgard II® cotton is necessary to ensure the future success of GM cotton. / Thesis (M. Environmental Science)--North-West University, Potchefstroom Campus, 2012.

Genetically modified (GM) cotton expressing Cry1Ac proteins was released in South Africa in 1997 for control of the bollworm complex on this crop. No reports of the failure of Bollgard® cotton to control these pests have yet been made. Throughout the world there are concerns about the development of resistance of target pests to Bt cotton due to the use of only one Bt gene. The aim of this study was to determine if Helicoverpa armigera (Lepidoptera: Noctuidae) and Diparopsis castanea (Lepidoptera: Noctuidae) developed resistance to Bt cotton in South Africa. To determine if H. armigera developed resistance, laboratory experiments were conducted to determine the levels of larval survival and development time when feeding on Bt and non–Bt cotton. Bollworm populations were collected on maize and cotton at different sites in South Africa and reared on Bt and non–Bt cotton under laboratory conditions. Results showed that some populations survived on Bt cotton and that a significant proportion of the individuals successfully completed their life cycles on Bt cotton. Surveys were also conducted amongst cotton farmers to determine the levels of compliance to the refuge strategy that has to be implemented by farmers as an insect resistance management (IRM) strategy to delay resistance development. The levels of compliance to refugia requirements were low and farmers generally only started planting refugia several years after they planted Bt cotton for the first time. The development of resistance of H. armigera to Bt cotton in South Africa can possibly be ascribed to non–compliance to the prescribed refuge requirements. No conclusions can be made on resistance of D. castanea to Bt cotton but the relatively long time to mortality of larvae could indicate development of tolerance to Cry1Ac proteins. The new generation Bollgard II® cotton, expressing both Cry1Ac and Cry2Ab2 proteins, has been released in South Africa during the 2010/11 growing season and field observations showed effective control of the bollworm complex at several sites in the country. Monitoring of refuge compliance levels as well as resistance development in the bollworm complex to Bollgard II® cotton is necessary to ensure the future success of GM cotton. / Thesis (M. Environmental Science)--North-West University, Potchefstroom Campus, 2012.

Thesis (MSc (Genetics))--University of Stellenbosch, 2010. / Includes bibliography. / ENGLISH ABSTRACT: Wheat rust is a significant contributor to the total impact of diseases on sustainable wheat production. Genetic resistance, produced by using resistance genes from wheat and other related wild species, is the simplest and most cost-effective way to guard against these diseases. The pyramiding of resistance genes in a single line is a vital practice in bringing about durable resistance. This study aimed to develop a series of doubled haploid (DH) wheat lines containing combination's of wild species genes for rust resistance. Rust resistance genes Lr19 (7BL), Sr31/Lr26/Yr9/Pm8 (1BS) and Lr54/Yr37 (2DL) were combined by means of crossing. Breeders. lines which have complex resistance including Lr24/Sr24 (3DL), Lr34/Yr18 (7D), Sr36 (2BS) and Sr2 (3BS), were used. Marker assisted selection (MAS) was used to type populations for the above mentioned genes. Using the DH method (maize pollination technique), an inbred population was developed from the selected lines, after which the lines were characterised molecularly for the resistance gene translocations which they contain. The study produced 27 lines with diverse genetic profiles. Seven lines contain four translocations (Lr24/Sr24, Lr34/Yr18, Sr2 and Lr19 or Sr31) each, 11 lines contain three genes each, six lines contain two genes each and only three lines contain a single translocation (Lr24/Sr24). The reality that rust pathogens have already overcome three of the resistance genes in the final population . Lr19, Sr31 and Sr24 . is a clear indication of the value of using non-major gene resistance for bringing about durable resistance. The focus should fall ever more greatly upon the application of quantitative trait loci (QTL) for this purpose, which will result in MAS contributing to the development of more durable resistance. The value of the integration of MAS and DH in combination with conventional breeding practices in breeding programmes has already been illustrated internationally for increasing the rate of cultivar development and this is reaffirmed by this study. / AFRIKAANSE OPSOMMING: Koringroes lewer jaarliks .n beduidende bydrae tot die totale impak van siektes wat volhoubare koringverbouing belemmer. Die mees eenvoudige en koste-effektiewe verweer teen hierdie siektes is genetiese weerstand, wat deur weerstandsgene vanaf koring, sowel as wilde verwante spesies, bewerkstellig word. Die stapeling van weerstandsgene in .n enkele lyn word as .n onontbeerlike praktyk om duursame weerstand tot stand te bring, geag. Hierdie studie het ten doel gehad om .n reeks verdubbelde haploiede (VH) koringlyne te ontwikkel wat kombinasies van wilde spesie gene vir roesweerstand bevat. Roesweerstandsgene Lr19 (7BL), Sr31/Lr26/Yr9/Pm8 (1BS) en Lr54/Yr37 (2DL) is deur middel van kruisings gekombineer. Telerslyne wat oor komplekse weerstand beskik wat Lr24/Sr24 (3DL), Lr34/Yr18 (7D), Sr36 (2BS) en Sr2 (3BS) insluit, is gebruik. Merker-bemiddelde seleksie (MBS) is gebruik om populasies vir bogenoemde gene te tipeer. .n Ingeteelde populasie is vanaf die geselekteerde lyne met behulp van die VH metode (mielie-bestuiwing tegniek) ontwikkel, waarna die lyne molekuler vir die weerstandsgeentranslokasies waaroor hul beskik, gekarakteriseer is. Die studie het 27 lyne met diverse genetiese profiele opgelewer. Sewe lyne bevat vier weerstandsgeentranslokasies (Lr24/Sr24, Lr34/Yr18, Sr2 en Lr19 of Sr31) elk, 11 lyne beskik oor kombinasies van drie gene elk, ses bevat twee gene elk en slegs drie lyne beskik oor .n enkele translokasie (Lr24/Sr24). Die realiteit dat die roespatogene reeds drie van die weerstandsgene in die finale populasie . Lr19, Sr31 en Sr24 . oorkom het, benadruk die waarde van die gebruik van nie-hoofgeenweerstand vir die daarstelling van duursame weerstand. Die fokus behoort toenemend meer op die aanwending van kwantitatiewe kenmerk-loci (QTL) vir hierdie doel te val en sal sodoende teweegbring dat MBS bydra tot die ontwikkeling van meer duursame weerstand. Die waarde van die integrasie van MBS en VH in kombinasie met konvensionele telingsmetodiek is reeds internasionaal vir die versnelling van kultivarontwikkeling aangetoon en word ook deur hierdie studie herbevestig.