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Made available in DSpace on 2012-05-07T14:48:00Z (GMT). No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) 000042.pdf: 10539683 bytes, checksum: ec9117c60b114e25414559e13e373a07 (MD5) Previous issue date: 2009 / A dissertação tem por objetivo apresentar as medidas para controle e erradicação da bouba ocorridas no Brasil no período entre 1956 e 1961. O Programa de Erradicação da bouba -com seu método de injeções únicas de penicilina - percorreu em campanha itinerante os estados do nordeste e de Minas Gerais em seus primeiros cinco anos. Durante as atividades da campanha nas principais áreas endêmicas do país emergiram também outras questões relacionadas à alimentação, pobreza e condições de vida das populações do interior. O trabalho ressalta como uma campanha para a erradicação de uma endemia rural estruturada em torno de uma bala mágica, a penicilina injetável, se defrontou com os quadros de fome e desnutrição no interior do país, problemas que, a princípio, estavam fora de suas atribuições.

Bioarchaeologists and palaeopathologists have recently turned their attention towards one critical aspect of the study of the history of disease: health-related caregiving. In response, an approach, the bioarchaeology of care, and, within it, the web-based Index of Care (IoC) have been developed to enable the identification and interpretation of past caregiving. Here, we apply the IoC to Burial 86, a young adult (18–25 years) female from the late Mississippian period, Dallas cultural phase Holliston Mills site (40HW11; ca. ad 1348–1535), TN. Burial 86 exhibits pathologies specific to treponematosis. They also exhibit a suite of pathologies indicative of physical impairment, including a varus angular deformity in the right tibia that is potentially the result of a malaligned pathological fracture. Following the IoC, we determine that Burial 86 probably experienced moderate clinical impacts on several domains (e.g., musculoskeletal system) with various functional impacts on essential activities of daily living. This means that Burial 86 likely had a disability and likely received caregiving, though it is impossible to determine if the care was efficacious. That care was provided likely reflected the community of Holliston Mills' more egalitarian socio-political structure, which was unusual for the late Mississippian. It may also reflect Burial 86's agency, the presence of adequate resources at the site, as indicated by high frequencies of high status mortuary artifacts, or a combination of these factors. The mortuary program for Burial 86 does not indicate that they were marked as being different—in status or other social categories—than other community members. This study highlights how bioarchaeological evidence can be used to explore the downstream effects of chronic infections, such as treponematosis, throughout the body and across the life course, and the opportunities for health-related caregiving in past societies that these processes can potentially create.

<div>The eradication of yaws a neglected tropical disease caused by Treponema pallidum subsp. pertenue, which affects children living in very deprived hard to reach rural communities is constrained by the lack of rapid, accurate diagnosis. I sought to develop a molecular point-of-care test for the diagnosis of yaws. A Loop-mediated isothermal amplification (LAMP) assay with primers targeting the conserved gene, tp0967, with visual detection by lateral flow test strip was developed and optimized. The limit of detection was evaluated while 63 samples from clinical cases of yaws and 5 samples with PCR-confirmed syphilis were used to determine the sensitivity and specificity of the assay compared to the current molecular testing protocol. Reagents were dried in tubes and tested up to 14 days. The developed LAMP assay was found to be optimal when run at 65oC in a water bath for 30 minutes. The limit of detection was 2.7*104 DNA copies/ml. The sensitivity of the LAMP assay using unextracted and DNA extracted samples were 0.67 and 1.00 respectively. None of the syphilis samples tested positive in any of the assays. We show the development of a fast and sensitive LAMP assay for yaws detected by lateral flow test strip. Using extracted DNA, the assay sensitivity is at par with gold standard detection. The assay can be adapted to minimal sample processing required for in-field detection without DNA extraction.</div><div><br></div>