The experiment was based on the feeding of feed additive, which was composed of vitamins, methionine, zinc and copper in organic form (amino acid complex n-hydrate). The experiment was 14 horses divided into two groups (test and control group). The experimental group was the basic ration, which also received a control group, added over a period of nine months, a feed additive. During the experiment, 5 samples were taken from which the detected concentration of some mineral elements (Ca, Fe, Mg, Cu, Zn) and various biochemical parameters (glucose, aspartate aminotransferase, urea, bilirubin, alkaline phosphatase). Administration of a feed additive was a statistically significant (P <0.05) increase in the zinc content of the original 5828 mg +- 1.67 10.57 +- 0.79 four milligrams. The copper content in the control sampling was 10.737 +- 2.46 and statistically significantly increased to 12.698 +- 1.74 mg. Check sampling contained 4.58 +- 0.56 mmol / l glucose, the last collection and showed no significant difference statistically insignificant (P <0.01) was 4.45 +- 0.46 mmol/l. In the control sampling was determined the average number of aspartate aminotransferase to 2.46 +- 0.48 mikromol/l, which is due to the administration of feed additives statistically insignificant (P <0.01) increased to 3.37 +- 0.71 mikromol/l.. Blood urea is statistically insignificant (P <0.01) did not change much, the initial value of 5.86 +- 1.20 mmol / l decreased to 5.75 +- 1.17 mmol / l. Bilirubin is statistically insignificant (P <0.01) without significant deviation from the initial 14.61 +- 5.45 mikromol/l slightly increased to 14.78 +- 6.41 mikromol/l.. Alkaline phosphatase statistically significant (P <0.05) increased from 1.98 +- 0.34 mikromol/l to 3.18 +- 0.66 mikromol/l.

This research investigates the ability of rat tapeworm (Hymenolepis diminuta) to accumulate zinc in tissue and the influence of its intake and excretion by its host (laboratory rat). The host was fed by food with zinc in two forms: 1) a mixture of standard food ST1 with hyperaccumulator plant Arabidopsis halleri, 2) ST1 mixture with zinc lactate, which is ordinarily used as a feed supplement for increasing zinc content and it is often included in human diet supplements. Rat control group fed by ST1 only was included in the experiment for verification of the difference. Rats were divided to six groups (OO, OT, RT, RO, MO a MT). Three rat groups were infected by rat tapeworm (OT, RT, MT) and three rat groups were not infected (OO, RO, MO). The control groups OO and OT were fed by ST1 only. RO and RT groups were fed by ST1 with admixture of Arabidopsis halleri and groups MO and MT were fed by ST1 with zinc lactate. Urine and excrements of the rats were collected twice a week during the experiment and their amounts were measured every day. The rats were weighted every week. At the end of the balance phase of the experiment rats were euthanized and seven selected tissues were removed (liver, kidney, spleen, small intestine, testis, muscle and bone). Rat tapeworm was removed from the infected rats. Blood was drawn from the rats. The results show that rat groups infected by the rat tapeworm had lower concentration of zinc in almost all analyzed tissues except for spleen, where the concentration of zinc was the same as in groups without the rat tapeworm. Based on the results the rat tapeworm also has an influence on the excretion of feces and urine.

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No description available.

In the first experiment we verified methodical procedure for conducting an experiment in the experimental stable of Mendel University in Brno in Zabcice. Further at work we observed the influence feeding two forms of zinc (inorganic -- zinc oxide and organic -- zinc bonded to methionine) on digestibility of basic nutrients of diets, on blood parameters and body condition as indicator of monitored dairy cows health. Dairy cows were observed in transitional phase and during lactation. Dairy cows fed by diet with inorganic form of zinc (called group A), had average digestibility of crude protein, fat, crude fiber, nitrogen-free extracts and zinc lower than group with organic form of zinc in diet (called group O). For example group A had digestibility coefficient of crude protein 69.3 +- 7,80 % and zinc 60.4 +- 8.35 % during of the whole observation, while group O had digestibility coefficient of crude protein 75.0 +- 6.15 % and zinc 68.4 +- 5.57 %. Statistically significant differences are shown in the number of red blood cells (RBC) - group A had before calving, on day of calving, on 30th and on 60th day after calving statistically significantly higher (P <0.01) levels of RBC than group O, which had an effect on the value of hematocrit in the same period (P <0.05 and P <0.01) and hemoglobin on the day of calving and on 60th day after calving (P <0.05). Average daily intake of dairy cows were almost same. We observed lower decrease weight and body condition of dairy cows (group O). Group A decreased weight from 744.4 kg before calving on 610.8 kg on 60th day after calving compared to group O from 732.8 kg (before calving) on 646.9 kg (60th day after calving). It average fall condition about 0.8 BCS (group A), while only about 0.5 BCS (group O).

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The aim of this study was to investigate factors of mineral nutrition influencing growth and composition of skin derivates and their content in blood plasma of horses. The thesis describes three experiments carried out on Czech warm blood breed horses on Boudky farm, Velké Němčice. In the first experiment was analysed the effect of fed supplement mixture -- vitamins, methionine and organic form of zinc and copper -- on growth rate and mineral composition of hoof horn and hairs and level of trace elements in blood plasma. The effects of copper and zinc in their organic and inorganic form on parameters above were evaluated in the second and third experiment. The addition of supplement mixture to experimental group led to significant increasing (P<0.01) level of zinc in dry matter of hoof horn from 95.82 +- 8.96 mg.kg-1 to 117.73 +- 10.23 mg.kg-1 and copper (P<0.05) from 2.39 +- 0.75 mg.kg-1 to 4.06 +- 1.14 mg.kg-1 during the first experiment. No differences were found between initial and final levels of minerals in hairs. In the second experiment, mares receiving copper sulphate deposited significant higher (P<0.01) copper level into hairs 17.39 +- 2.32 mg.kg-1 than the mares of control group 13.37 +- 1.82 mg.kg-1. The results of mares receiving copper in organic form did not significantly differ from the previous two groups. There was found out significantly decreased (P<0.05) levels of zinc content in dry matter of hair from 153.56 +- 10.46 mg.kg-1 to 139.68 +- 7.09 mg.kg-1 in the mares receiving of zinc oxide and in the mares of control group from 154.59 +- 17.03 mg.kg-1 to 136.33 +- 6.96 mg.kg-1 (P<0.05) during experiment. The growth rate of skin derivates was influenced only by using supplement mixture -- vitamins, methionine and organic form of zinc and copper and was not influenced with individual application of copper or zinc. Growth rate of hoof horn in the mares receiving this supplement was significantly higher (P<0.01) about 22.31 % than in the animals of control group. The same results was found in the mane hairs (P<0.01). The growth rate of hairs was about 15.37 % higher in the horses from experimental group than from the control one.

No description available.

Zinc is widely used as an alternative to antibiotic in piglets. Recent studies demonstrated the antibacterial effect of zinc nanoparticles. It is believed that zinc nanoparticles will exhibit better antibiotic effects than previously used medication zinc. There is a need to define ZnNPs effect on the organism. This thesis compares the influence of administration of zinc oxide nanoparticles and zinc standard forms at the dose of 2000 mg/Zn/kg diet in rat organism. The antioxidant status of rats and toxicity of zinc and zinc nanoparticles were monitored. The level of accumulated zinc for blood was on average 12 mg / kg ± 6 mg / kg, for liver 74 mg / kg ± 50 mg / kg, for kidneys 37 mg / kg ± 30 mg / kg for both nanoparticles and normal zinc . ZnNPs and zinc oxide did not extremely influence antioxidant status of samples of blood, liver and kidney. A significant effect was observed with GSH levels. Histological study of liver and duodenum showed slight tissue damage after administration of both zinc and nanoparticles. Zinc nanoparticles demonstrated beneficial effects as well as standard form.

Zinc is an essential element that has many important functions in our organism. The objective of the thesis is to verify the effect of dietary zinc nanocomplexes as an alternative source of zinc for the animal organism. For the experimental model of this experiment male rats of the outbred strain Wistar albino were used. The rats were divided into 3 groups. Six males were housed in each group. The first group (control) (n = 6) of the rats served as a control one and the zinc dose was not increased. The second group (Zn-EDT) of animals (n = 6) was dosed with zinc nanoparticles (200 mg/kg diet). The third group (Zn-NTA) of rats (n = 6) was dosed with zinc in the form of zinc nanoparticles (200 mg/kg diet). The rats were sacrificed after 15 days of experimentation. The animals samples of erythrocytes and liver were extracted and subjected to appropriate analyses immediately after collection. Antioxidant activity was analysed using FR and DPPH methods, SOD activity, metallothionein and zinc concentration. The results show that the antioxidant capacity in the erythrocytes decreased statistically in Zn-NTA (P < 0.05). The concentration of zinc in erythrocytes has increased inconclusively. There was a statistically significant increase (P < 0.05) of metallothionein in the liver.