Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-05-19T13:08:14Z
No. of bitstreams: 1
TES_LUIZ_CARLOS_PORCELLO_MARRONE_COMPLETO.pdf: 11632116 bytes, checksum: 2546c03c25eed68601cf00144a3d5888 (MD5) / Made available in DSpace on 2016-05-19T13:08:14Z (GMT). No. of bitstreams: 1
TES_LUIZ_CARLOS_PORCELLO_MARRONE_COMPLETO.pdf: 11632116 bytes, checksum: 2546c03c25eed68601cf00144a3d5888 (MD5)
Previous issue date: 2016-03-11 / Introduction: The Posterior Reversible Encephalopathy Syndrome (PRES) is a clinicoradiologic entity characterized by headache, altered level of consciousness, seizures and visual abnormalities, which is associated with an encephalic white matter edema, predominantly affecting the occipital and parietal lobes. The pathophysiology of PRES remains unclear. In a previous study, we developed an experimental model to better understand the cerebral changes of this syndrome in cases related to pregnancy disorders. The use of microPET-CT with 18F-FDG is a way of assessing brain metabolism changes related to PRES, allowing perform a follow up of these changes in subsequent exams. Objectives: The aim of this study is to evaluate the changes of the glucose cerebral metabolism and the permeability of blood brain barrier in pregnant rats submitted to Reduced Uterine Perfusion Pressure (RUPP) in comparison with pregnant control rats. Methods: Pregnant rats were divided in two groups: RUPP group and control group. Animals of the RUPP group underwent the procedure to reduce uteroplacental perfusion pressure. The animals of both groups were submitted to two microPET-CT scans with 18F-FDG (before and after the delivery) and then, we evaluate the permeability of blood brain barrier using Evans blue. Results: Evaluating the results of microPET-CT scans with 18F-FDG in the ten animals that were submitted to RUPP procedure in comparison with the six pregnant controls animals, there is a cerebral hypometabolism in the exam realized in period before the delivery in the group of rats underwent RUPP. In the postpartum period this result is reversed and the group of animals subjected to the procedure presents a cerebral hypermetabolism when compared to control group. Evaluating the permeability of the blood-brain barrier with Evans blue, 8 of 10 animals (80%) that were submitted to RUPP procedure had the pigment in brain parenchyma, which was not found in any of six animals of the control group. Conclusion: In this study we described a pattern of lower brain metabolism in Wistar rats submitted to RUPP procedure when compared to pregnant control rats before the delivery using microPET-CT with 18F-FDG for this evaluation. After delivery, there is an inversion and the group of animals submitted to RUPP procedure becomes hypermetabolic when compared to the control group using the same technique for measurement of glucose metabolism. The Evans Blue impregnating maintains the same brain tissue after passing seven days of the completion of delivery, and microscopic changes also persist. / Introdu??o: A S?ndrome da Encefalopatia Revers?vel Posterior (Posterior Reversible Encephalopathy Syndrome - PRES) ? uma entidade clinico-radiol?gica caracterizada por cefal?ia, altera??o do n?vel de consci?ncia, crises convulsivas e altera??o visual, a qual est? associada a edema na subst?ncia branca encef?lica, predominantemente acometendo os lobos occipitais e parietais. A fisiopatologia do PRES permanece desconhecida. Em estudo pr?vio desenvolvemos modelo experimental para melhor entender as altera??es cerebrais dessa s?ndrome nos casos relacionados a dist?rbios da gesta??o. O uso de microPET-CT com 18F-FDG ? um modo de avaliar as modifica??es de metabolismo cerebral relacionadas ao PRES, permitindo o acompanhamento dessas modifica??es em exames subsequentes. Objetivos: O objetivo desse trabalho ? avaliar as modifica??es do metabolismo de glicose cerebral e da permeabilidade da barreira hematoencef?lica em ratas gravidas submetidas ao procedimento RUPP em rela??o a ratas gravidas controle. M?todos: Ratas gravidas foram divididas em dois grupos: grupo RUPP e grupo controle. Os animais do grupo RUPP realizaram o procedimento para redu??o da press?o de perfus?o ?tero-placent?ria. Os animais dos dois grupos foram submetidos a dois exames de microPET-CT com 18F-FDG (antes e ap?s o parto) e, posteriormente a avalia??o da permeabilidade da barreira hematoencef?lica com uso de azul de Evans. Resultados: Avaliando-se o resultado dos exames de microPET-CT com 18F-FDG nos dez animais que foram submetidos ao procedimento da RUPP em rela??o aos seis animais controles encontramos que ocorre um hipometabolismo nas ratas que foram submetidas ? RUPP no per?odo antes do parto. J? no per?odo de puerp?rio, esse resultado se inverte e o grupo de animais submetido ao procedimento apresenta um hipermetabolismo quando comparado ao controle. Ao avaliar-se a permeabilidade da barreira hematoencef?lica ao azul de Evans, encontramos que em 8 dos 10 animais (80%) submetidos ? RUPP havia a presen?a do pigmento, o qual n?o foi encontrado em nenhum dos seis animais do grupo controle. Conclus?o: Nesse estudo encontramos um padr?o de menor metabolismo cerebral em ratas Wistar submetidas ? RUPP quando comparado ?s ratas do grupo controle antes da realiza??o do parto utilizando microPET-CT com 18F-FDG para essa avalia??o. Ap?s o parto, ocorre uma invers?o e o grupo de animais submetidos ? RUPP passa a ser o de maior metabolismo em compara??o aos animais do grupo controle utilizando a mesma t?cnica para aferi??o do metabolismo de glicose. O azul de Evans mant?m impregnando o tecido cerebral mesmo ap?s a passagem de sete dias da realiza??o do parto, assim como as altera??es microsc?picas tamb?m persistem.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6681 |
| Date | 11 March 2016 |
| Creators | Marrone, Luiz Carlos Porcello |
| Contributors | Costa, Jaderson Costa da |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, Brasil, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 600, 600, 600, -8624664729441623247, -969369452308786627 |
Page generated in 0.0024 seconds