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Previous issue date: 2013-08-26 / During the neonatal period, the administration of lipopolysaccharide (LPS) generates a stressful immune stimulation resulting in the expression of cytokines in the central nervous system. As result, brain changes in certain regions of the hippocampus of adult rats, as well as activation of the hypothalamic-pituitary-adrenal axis (HPA) in neonates is observed. The objective of this study is to evaluate the long-term effects of neonatal inflammation on the oxidative and inflammatory status during experimental sepsis in adult life. On day ten after birth, Balb/c mice received different treatments: nLPS, received 100 μL of a 100 μg/kg i.p. LPS solution or nSal, received 100 μL of i.p. saline solution. As adults, animals were submitted to i.p. administration of saline solution or LPS. After 12 hours, serum samples and liver were collected to measure inflammatory cytokines and to determine mitochondrial complexes and oxidative stress, respectively. There was an increase in the fear/anxiety behavior in the nLPS group. The complexes II and II-III increased in the nLPS saline group when compared to control. The LPS administration in the adult females induced a decrease of the glutathione enzyme activity. There were no differences in the inflammatory cytokines. The results indicate that the neonatal inflammation was effective in inducing programming. The mitochondrial respiratory chain metabolism seems to be influenced by the neonatal inflammation in males. LPS neonatal stress seems not to change the cytokine inflammatory profile in an experimental model of sepsis / A administra??o de lipopolissacar?deo (LPS) no per?odo neonatal gera um est?mulo imunol?gico estressante capaz de estimular a express?o de citocinas no sistema nervoso central e causar altera??es cerebrais em certas regi?es do hipocampo de ratos adultos, al?m de ativar o eixo hipot?lamo-pituit?ria-adrenal (HPA) em neonatos. O objetivo deste estudo ? avaliar os efeitos em longo prazo da inflama??o no per?odo neonatal sobre o perfil inflamat?rio e oxidativo na sepse experimental na vida adulta. No d?cimo dia ap?s o nascimento, camundongos Balb/c receberam diferentes tratamentos: nLPS, recebeu 100 L de solu??o 100 g/Kg de LPS i.p. ou nSal em que foi administrado 100 L de solu??o salina i.p. Quando adultos, os animais foram submetidos ? administra??o i.p. de solu??o salina ou LPS e, 12 horas ap?s, foram coletados o soro para mensura??o das citocinas inflamat?rias e o f?gado para determina??o dos complexos mitocondriais e estresse oxidativo. Houve aumento do comportamento de medo/ansiedade no grupo nLPS. Os complexos II e II-III aumentaram nos machos nLPS-salina quando comparados ao controle. A administra??o de LPS nas f?meas adultas provocou uma diminui??o da atividade da enzima glutationa reduzida. N?o houve diferen?as em rela??o ?s citocinas inflamat?rias. Os resultados indicam que a inflama??o no per?odo neonatal foi efetiva na indu??o do programming. O metabolismo da cadeia respirat?ria mitocondrial parece ser influenciado pela inflama??o neonatal em animais machos. O estresse neonatal por LPS parece n?o alterar o perfil inflamat?rio das citocinas no modelo de sepse experimental.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1424 |
| Date | 26 August 2013 |
| Creators | Lunardelli, Adroaldo |
| Contributors | Donadio, M?rcio Vin?cius Fagundes |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina/Pediatria e Sa?de da Crian?a, PUCRS, BR, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 3098206005268432148, 500, 600, -8624664729441623247 |
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