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Previous issue date: 2015-08-31 / Background: Invariant natural killer T (iNKT) cells play complex functions in the
immune system, releasing both Th1 and Th2 cytokines. The role of iNKT cells in human
asthma is still controversy and never described in severe therapy asthmatic children
(STRA).
Objective: To analyse iNKT frequency in peripheral blood of children with severe
therapy-resistant asthma (STRA), comparing with children with milder asthma and healthy
controls.
Methods: This is a case-control study where blood was collected from asthmatic children
(n=136) (milder and STRA) and healthy controls (n=40). Peripheral blood mononuclear
cells (PBMC) were isolated, stained with anti-CD3 and anti-iNKT (V?24J?18), and
analysed through flow cytometry. Atopic status was defined by measuring specific IgE in
serum. Airway inflammation was assessed through induced sputum.
Results: Children with asthma (n = 136) presented an increased frequency of CD3+iNKT+
cells (mean 2.31% SD 4.41), compared to healthy controls (n = 40) (mean 0.48% SD 0.79)
(p = 0.02). Mild-asthmatic children (n = 99) showed an increased frequency of iNKT cells
(2.65% SD 4.91; p = 0.002) compared to STRA (n = 11) (2.05% SD 1.62). STRA children
have shown an increase frequency of iNKT cells (2.05% SD 1.62) than controls (0.48%
SD 0.79; p = 0.002). The number of iNKT cells was not different between atopic (n = 127)
and non-atopic (n = 45) children. In addition, iNKT cells were not associated with any
inflammatory pattern of induced sputum studied classified as eosinophilic (n = 18),
neutrophilic (n = 23), mixed (n = 31) (eosinophilic and neutrophilic) and paucigranulocytic
(n = 5). However, iNKT cell numbers were not associated with any airway
inflammatory pattern (p=0.09)
Conclusion and Clinical Relevance: Our data suggested a role to iNKT in paediatric
asthma since it increased in asthmatic children; however these cells are not associated with
atopic status or with the more severe spectrum of the disease. / Introdu??o: C?lulas invariante de c?lulas NK (iNKT) desempenham fun??es complexas
no sistema imune, liberando citocinas do tipo Th1 e Th2. O papel das c?lulas invariante de
c?lulas NK (iNKT) na asma em humanos ainda ? controverso e nunca foi descrito em
crian?as com asma grave resistente ? terapia (AGRT).
Objetivo: Analisar a frequ?ncia de c?lulas iNKT no sangue perif?rico de crian?as com
asma grave resistente ? terapia (AGRT), comparando com crian?as com asma leve e
controles saud?veis.
M?todos: Este ? um estudo de caso-controle onde foi coletado sangue de crian?as
asm?ticas com asma leve e AGRT (n=136) com idade entre 11.01?1.69 e controles
saud?veis (n=40) com idade entre 11.21?1.16 anos. A fra??o mononuclear do sangue
perif?rico (PBMC) foi isolada, marcada com anti-CD3+ e anti-iNKT (V?24J?18) e
analisada no cit?metro de fluxo. A atopia foi definida atrav?s da medi??o dos n?veis
plasm?ticos de IgE. A inflama??o das vias a?reas foi avaliada atrav?s da expectora??o
induzida.
Resultados: As crian?as com asma (n = 136) apresentaram um aumento da frequ?ncia de
c?lulas iNKT+CD3+ (m?dia: 2.31%?4.41), quando comparadas com os controles saud?veis
(n=40) (m?dia: 0.48%?0.79 p=0.02). Crian?as com asma leve (n = 99) apresentaram um
aumento da frequ?ncia de c?lulas iNKT (2.65%?4.91; p = 0.002) quando comparadas com
as crian?as com AGRT (n = 11) (2.05%?1.62). Crian?as com AGRT mostraram uma
aumento da frequ?ncia das c?lulas iNKT (2.05%?1.62) quando comparadas com os
controles saud?veis (0.48%?0.79; p = 0.002). O n?mero de c?lulas iNKT n?o foi diferente
entre at?picos (n = 127) e n?o-at?picos (n = 45), com m?dia de 2.25% ? 4.55 e 1.01% ?
1.46 respectivamente. Al?m disso, as c?lulas iNKT n?o foram associadas com nenhum
padr?o inflamat?rio (eosinof?lico n = 18; neutrof?lico n = 23; misto n = 31; paucicelular n =
5) estudado no escarro induzido (p=0.09).
Conclus?o e Relev?ncia Cl?ncia: Nossos dados sugerem que as c?lulas iNKT
desempenham um papel na asma pedi?trica, uma vez que estava aumentada nas crian?as
asm?ticas; entretanto, essas c?lulas n?o est?o associadas com a atopia ou com o espectro
mais severo da doen?a.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6464 |
| Date | 31 August 2015 |
| Creators | Antunes, Liana |
| Contributors | Pitrez, Paulo M?rcio Condessa, Duarte, Ana Paula |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina/Pediatria e Sa?de da Crian?a, PUCRS, Brasil, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 3098206005268432148, 600, 600, 600, -8624664729441623247, -969369452308786627 |
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