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Previous issue date: 2015-11-20 / Introduction: The etiology of sarcopenia is not well defined; studies suggest that different factors contribute to the development of this, including hormonal changes, loss of motor neurons, physical inactivity and use of medications. Another factor that may be associated together with oxidative stress is the frequency of micronucleus. However, to date it has not been reported in the literature studies investigating the association between the frequency of micronuclei and sarcopenia. Aim: to verify the association between biochemical markers, of redox metabolism, micronuclei frequency and sarcopenia in elderly residents in Uruguaiana city, RS. Methods: descriptive, comparative and exploratory cross-sectional study. The sample consisted of elderly treated at primary health care in the municipality of Uruguaiana, RS. The variables collected and investigated were divided into sociodemographic, anthropometric, haematological, biochemical (glucose, total cholesterol, triglycerides, high density lipoprotein linked to cholesterol (HDL-C), hepatic function (AST and ALT), renal function (urea, total protein and creatinine), and for REDOX metabolism (enzyme superoxide dismutase (SOD), catalase (CAT) activity, glutathione peroxidase (GPX), thiobarbituric acid-reactive substances (TBARS) and protein carbonylation), DNA damage (micronucleis frequency test). For determination of biomarkers venous blood samples were collected. For the anthropometric measurements were used: scale, stadiometer, tape measure and caliper. The tracking of sarcopenia was determined by the Lee formula: MM = height2 x (0.00744 x arm circumference2 + 0.00088 x thigh circumference2 + 0.00441 x calf circumference2) + 2.4 x sex - 0.048 x age + race + 7.8 and IMM = MM (kg)/ height (m) 2. And a correction was made to the subtraction of subcutaneous fat using the formula Cm = Climb_?S. To evaluate the functionality were applied tests: gait speed and grip strength with Jamar dynamometer. Results: the sample consisted of 168 elderly people, 62 men and 106 women, mean age 68.41 ? 6.13 years. Of these 49 (29.2%) were considered sarcopenic. The average levels of biochemical markers among non sarcopenic and sarcopenic groups were: Glucose 107.52 ? 49.46 and 103.57 ? 45.93 mg/ dL (p = 0648), total cholesterol 197.47 ? 46.93 and 177.43 ? 30.01 mg / dL (p = 0.010), HDL-C 47.53 ? 5.82 and 46.52 ? 6.55 mg / dL (p=0.354), triglycerides 160.30 ? 127.00 and 101.17 ? 66.55 mg / dl (p = 0.46). The means of REDOX metabolism markers among non sarcopenic and sarcopenic groups were: SOD = 0.1670 ? 0.05877 and 0.0916 ? 0.04585 U/ mg protein (p = 0.001), CAT = 2.0960 ? 0.48434 and 1.9720 ? 0.68767 U / mg protein (p = 0193), GPX = 1655.57 ? 191.61 and 1463.02 ? 228.11U / mg protein (p = 0.001), TBARS = 62.4983 ?16.07 e 83.2139 ? 21.50 nmol MDA / ml plasma (p = 0.001), protein carbonylation = 1.7019? 0.52 e 1.9506?0.67 nmol carbonyl / mg protein (p = 0.014), and the frequency of micronucleis = 5.50 ? 2.66 and 6.79 ? 2.84 (%) (p = 0.009). Conclusion: sarcopenic elderly have significantly higher average micronucleus frequency of lipid peroxidation (TBARS), protein carbonyls, on the other hand have also significantly lower average of antioxidant enzymes SOD, GPx, CAT and total cholesterol than non sarcop?ncios elderly. / Introdu??o: A etiologia da sarcopenia ainda n?o est? bem definida, estudos sugerem que diferentes fatores contribuem para o desenvolvimento desta, incluindo altera??es hormonais, perda de neur?nios motores, inatividade f?sica e uso de medicamentos. Outro fator que pode estar associado em conjunto com o estresse oxidativo ? a frequ?ncia de micron?cleo (FM). No entanto, at? o presente momento n?o foi relatado na literatura estudos que tenham investigado a associa??o entre a FM e a sarcopenia. Objetivo: verificar a associa??o entre marcadores bioqu?micos, do metabolismo REDOX, FM e sarcopenia em idosos residentes do Munic?pio de Uruguaiana, RS. M?todos: estudo transversal descritivo, comparativo e explorat?rio. A amostra foi composta por idosos atendidos na aten??o b?sica de sa?de do Munic?pio de Uruguaiana, RS. As vari?veis coletadas e investigadas foram divididas em sociodemogr?ficas, antropom?tricas, funcionais, hematol?gicas, bioqu?micas (glicose, colesterol total, triglicer?deos, lipoprote?na de alta densidade ligada ao colesterol (HDL-c), fun??o hep?tica (TGO e TGP), fun??o renal (ur?ia, prote?nas totais e creatinina) e do metabolismo REDOX (enzima super?xido dismutase (SOD), catalase (CAT), glutationa peroxidase (GPX), rea??o do ?cdio tiobarbit?rico (TBARS) e carbonila??o de prote?nas), dano de DNA (teste de FM). Para determina??o dos biomarcadores foram coletadas amostras de sangue venoso. Na avalia??o antropom?trica foram utilizados: balan?a, estadi?metro, fita m?trica e adip?metro. O rastreio da sarcopenia foi determinado pela f?rmula de Lee: MM= estatura2 x (0,00744 x circ. bra?o2+0,00088 x circ.coxa2+0,00441 x circ.panturrilha)2 + 2,4 x sexo ? 0,048 x idade + ra?a + 7,8) e IMM= MM(kg)/Estatura (m)2. E foi realizada uma corre??o para a subtra??o da gordura subcut?nea atrav?s da f?rmula Cm = Climb_?S. Para avalia??o da funcionalidade foram aplicados os testes: velocidade da marcha e for?a de preens?o manual com o dinam?metro Jamar. Resultados: A amostra foi composta por 168 idosos, 62 homens e 106 mulheres, com idade m?dia de 68,41?6,13 anos. Destes 49 (29,2%) foram considerados sarcop?nicos. A m?dia dos n?veis dos marcadores bioqu?micos, entre os grupos n?o sarcop?nico e sarcop?nico foram: glicose 107,52 ? 49,46 e 103,57 ? 45,93 mg/dL (p=0,648), colesterol total 197,47 ? 46,93 e 177,43 ? 30,01 mg/dL (p=0,010), HDL-c 47,53 ? 5,82 e 46,52 ? 6,55 mg/dL (0,354), triglicer?deos 160,30 ? 127,00 e 101,17 ? 66,55 mg/dL (p=0,46). As m?dias dos marcadores do metabolismo REDOX entre os grupos n?o sarcop?nico e sarcop?nico foram: SOD=0,1670 ? 0,05877 e 0,0916 ? 0,04585 U/mg prote?na (p=0,001), CAT= 2,0960 ? 0,48434 e 1,9720 ? 0,68767 U/mg prote?na (p=0,193), GPX= 1655,57? 191,61 e 1463,02 ? 228,11U/mg prote?na (p=0,001), TBARS= 62,4983 ?16,07 e 83,2139 ? 21,50 nmol MDA/mL plasma (p=0,001), carbonila??o de prote?nas= 1,7019 ? 0,52 e 1.9506?0.67 nmol carbonyl/mg prote?na (p=0,014), e FM = 5,50 ? 2,66 e 6,79 ? 2,84/1000 (p=0,009). Conclus?o: idosos sarcop?nicos apresentam significativamente m?dias maiores de freq??ncia de micron?cleo, de peroxida??o lip?dica (TBARS), carbonila??o de prote?nas, em contrapartida apresentam tamb?m significativamente menores m?dias das enzimas antioxidantes SOD, GPx, CAT e colesterol total do que idosos n?o sarcop?ncios.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6446 |
| Date | 20 November 2015 |
| Creators | Tavares, Graziela Morgana Silva |
| Contributors | Gottlieb, Maria Gabriela Valle |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Gerontologia Biom?dica, PUCRS, Brasil, Instituto de Geriatria e Gerontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 4438661476953179033, 600, 600, 600, 2296420844541114010, -969369452308786627 |
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