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Previous issue date: 2006-05-04 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Fungal polysaccharides have received a great deal of attention due to itsbecause of their potential use in a wide rangegreat variety fromof industries. Some studies have demonstrated that polysaccharides extracted offrom basidiomycetes they have presented significant properties as anti-inflammatory, antimicrobial, antioxidant and anti-tumoral properties. In spite of thisDespite these potential properties, these mushrooms have not been insufficiently investigated, and the great number of antibiotics number produced forby these organisms suggests that they canmay be a new source of bioactives composites source. In tThe present work, reports onlated the chemical composition, potential antioxidant, antiinflammatory and citotoxycity of extracted polymers extracted offrom the fruits bodies of the fungiius Geastrum saccatum and Polyporus dermoporus, native mushrooms of the Atlantic forest inof the state of the Rio Grande do Norte, Brazil. The Cchemical analyses had revealed ademonstrated text of total sugar rates of 65% and 49%, and proteins of 7.0% for in extracts of G. saccatum and P. dermoporus extracts, respectively. The analyses ofNMR spectroscopy of RMN had demonstrated that these extracts are composites forof a complex involving β- glucans and- proteins complex. The inhibition of the formation of superoxide radicals formation was of 88.4% in G. saccatum and 83.3% in P. dermoporus, and 75 and 100% for inhibition of hydroxyls radicals inhibition. TopicalThe topic application of extracts the 10, 30 and 50 mg/kg extract in BALBc mice with cutaneous inflammation induced byfor croton oil demonstrated to inhibitedion of ear edema of ear and cells polimorfonuclears cells atin the inflamed siteplace, being this reply more effective in lower concentrations being more effective. The evaluation of the glucans of G. saccatum and P. dermoporus glucans under induced pleurisy for carrageenan-induced pleurisya of showed the antiinflammatory action of these composites., being analyzed tThe frame number in the pleural exudates and thedosage of nitric oxide dosage was also analyzed. The cytotoxic action of these polymers was analyzed throughthrough the mitochondrial function (MTT). The incubation of the glucans with mononuclear cells of the peripheral blood demonstrated that the extracted glucans extracted fromof G. saccatum havepossess a moderate cytotoxic action. These results suggest that these mushrooms possess polymers formed byfor a complex glucana-protein complex, with antiinflammatory and antioxidant actions / Polissacar?deos de fungos t?m recebido grande aten??o devido ao seu uso potencial numa grande variedade de ind?strias. V?rios estudos t?m demonstrado que polissacar?deos extra?dos de basidiomicetos t?m apresentado propriedades significantes como anti-inflamat?rio, antimicrobial , antioxidante e anti-tumoral. Apesar dessas propriedades potenciais, esses cogumelos t?m sido insuficientemente investigados, e o grande n?mero de antibi?ticos produzidos por esses organismos sugere que eles podem ser uma fonte de novos compostos bioativos. No presente trabalho, relatamos a composi??o qu?mica, potencial antioxidante, antiinflamat?rio e citotoxico de pol?meros extra?dos dos corpos de frutifica??o dos fungos Geastrum saccatum e Polyporus dermoporus, cogumelos nativos da mata atl?ntica do estado do Rio Grande do Norte. As an?lises qu?micas demonstraram teor de a??cares totais de 65% e 49%, prote?nas a 7,0% nos extratos de G. saccatum e P. dermoporus, respectivamente. As an?lises de espectroscopia de RMN demonstraram que esses extratos s?o compostos por um complexo envolvendo b - glucanas e por??es prot?icas. A inibi??o da forma??o de radicais super?xido foi de 88,4% G. saccatum e 83,3% em P. dermoporus, e 75 e 100% para inibi??o de radicais hidroxilas. A aplica??o dos extratos a 10, 30 e 50 mg/kg em camundongos BALBc com inflama??o cut?nea induzida por ?leo de cr?ton demonstrou inibi??o de edema de orelha e de c?lulas polimorfonucleares no local inflamado, sendo esta resposta mais efetiva em concentra??es mais baixas. A avalia??o das glucanas de G. saccatum e P. dermoporus sob pleurisia induzida por carragenana de montrou a??o antiinflamat?ria desses compostos, sendo analisado o n?mero de c?lulas no exudato pleural e dosagem de ?xido n?trico. A a??o citot?xica desses pol?meros foi analisada atrav?s da fun??o mitocondrial (MTT). A incuba??o das glucanas com c?lulas mononucleares do sangue perif?rico demonstrou que as glucanas extra?das de G. saccatum possuem um a??o citot?xica moderada. Estes resultados sugerem que esses cogumelos possuem pol?meros formados por um complexo glucana-prote?na, ativos como antiinflamat?rio e antioxidante
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12563 |
| Date | 04 May 2006 |
| Creators | Dore, Celina Maria Pinto Guerra |
| Contributors | CPF:06341462468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783254U6&dataRevisao=null, Silva, Maria da Paz Carvalho da, CPF:17264782468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781416A2, Ferreira, Aurigena Antunes Ara?jo, CPF:83806059420, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792980U2, Leite, Edda Lisboa |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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