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Previous issue date: 2013-03-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / In this work were synthesized and
characterized complexes of general formule [Pt(P)2Cl2] and [Pt(P)2(CQ)Cl]PF6
(where (P)2= triphenylphosphine (PPh3), 1,3-bis (diphenylphosphino ) propane
(dppp), 1,4-bis (diphenylphosphino) butane (dppb), 1,1 '-bis
(diphenylphosphino) ferrocene (dppf) and CQ = Chloroquine). The
characterization of the complexes was performed by means of elemental
analysis, conductivity, absorption spectroscopy in the infrared and UV-Visible
regions (IR and UV-Vis), NMR 1H, 13C{1H}, 15N{1H}, 31P{1H}, 195Pt{1H},
cyclic voltammetry and differential pulse, and mass spectrometry (in the case of
chloroquine complexes). Also, the interaction of the chloroquine complexes
with DNA was evaluated for calculate to the magnitude of the interaction with
this important macromolecule, obtaining that the complexes showed interaction
constants similar and higher than those presented by CQ and CQDP, and
decrease the relative viscosity of DNA. We also evaluated the cytotoxic activity
of the chloroquine complexes in the cell lines MCF-7 and MDA-MB-231
(human breast tumor cell lines), DU-145 (the human prostate tumor cell line),
A549 (the human lung adenocarcinoma epithelial cell line), V79-4 (the Chinese
hamster lung fibroblast cell line), and the L929 (nontumor cell line from mice),
showed promissory activity, mainly of the complexes 5 and 8. / Neste trabalho, realizou-se as síntese e caracterizações dos
complexos de formula geral [Pt(P-P)Cl2] e [Pt(P-P)(CQ)Cl]PF6 (onde P-P=
trifenilfosfina (PPh3), 1,3-bis(difenilfosfino)propano (dppp), 1,4-
bis(difenilfosfino)butano (dppb), 1,1´-bis(difenilfosfino)ferroceno (dppf), e
CQ=Cloroquina). As caracterizações foram realizadas por meio de analise
elementar, condutividade, espectroscopia de absorção na região do
infravermelho (IV), de RMN 1H, 13C{1H}, 15N{1H}, 31P{1H}, 195Pt{1H},
espectroscopia de absorção na região do UV-Visível, voltametria cíclica e pulso
diferencial, e espectrometria de massas (no caso dos complexos com CQ).
Também foi avaliada a interação dos complexos sintetizados com o ADN, com a
finalidade de estimar a magnitude da interação dos complexos sintetizados com
esta importante macromolécula, obtendo se que os complexos sintetizados
apresentam constantes de interação similares e superiores as apresentadas pela
CQ e CQDF, e diminuem viscosidade relativa do DNA. Também foi avaliada a
atividade citotóxica dos complexos com CQ nas linhagens celulares tumorais
MCF-7 e MDA-MB-231 (tumor de mama humano), DU-145 (tumor de próstata
humano), A549 (adenocarcinoma de pulmão humano) e não tumorais V79-4
(fibroblasto de pulmão de hamster chinese) e L929 (células não tumorais de
camundongo), os quais apresentarem atividade promissória, principalmente os
complexos 5 e 8.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufscar.br:ufscar/7962 |
| Date | 28 March 2013 |
| Creators | Villarreal Peña, Wilmer José |
| Contributors | Batista, Alzir Azevedo |
| Publisher | Universidade Federal de São Carlos, Câmpus São Carlos, Programa de Pós-graduação em Química, UFSCar |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFSCAR, instname:Universidade Federal de São Carlos, instacron:UFSCAR |
| Rights | info:eu-repo/semantics/openAccess |
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