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Previous issue date: 2013 / A presente disserta??o consiste em dois artigos cient?ficos, sendo um artigo original e outro de revis?o. Em um dos artigos, os autores fizeram uma revis?o da literatura sobre c?ncer e pr?polis, abordando a carcinog?nese experimental induzida quimicamente pelo 9,10-dimetil-1,2-benzantraceno (DMBA), a composi??o qu?mica da pr?polis e seus efeitos quimiopreventivos. O objetivo do artigo de pesquisa foi avaliar a rea??o tecidual da mucosa lingual de hamsters, submetida ? aplica??o di?ria e alternada de DMBA e extrato etan?lico de pr?polis (EEP) de uma apresenta??o comercial. Materiais e m?todos: 60 hamsters foram divididos em tr?s grupos, com dois per?odos experimentais, 13 e 20 semanas. A borda lateral da l?ngua foi submetida ? aplica??o t?pica, di?ria e alternada de DMBA 0,5 % e EEP 30 % (grupo EEP, n = 20), DMBA 0,5 % e extrato aquoso de pr?polis (EAP) (grupo EAP, n = 20), DMBA 0,5 % e soro fisiol?gico (grupo DMBA, n = 20). Realizou-se an?lise da ocorr?ncia dos tipos de altera??es cl?nicas e histol?gicas. Mensurou-se a ?rea e volume das altera??es cl?nicas, a ocorr?ncia das altera??es estruturais (AE) e citol?gicas (AC) do tecido epitelial escamoso com atipias e a ?rea histol?gica dos carcinomas de c?lulas escamosas (CCE). Para a an?lise estat?stica, aplicaram-se os testes ANOVA, qui-quadrado, Kruskal-Wallis e Mann-Whitney. Resultados: Para todas as vari?veis analisadas, n?o houve diferen?as significativas na compara??o entre os grupos nos dois tempos experimentais. Em 13 semanas ocorreu uma ?nica les?o de CCE no grupo EEP. Em 20 semanas, a maior ocorr?ncia de CCE tamb?m foi no grupo EEP. Conclus?o: o EEP, com teor alco?lico de 30 %, favoreceu a rea??o tecidual da inicia??o e da promo??o da carcinog?nese, por mecanismos ainda n?o elucidados. / ABSTRACT This dissertation consists of two papers, the first one is a review and the second is an original paper. In the first article, the authors reviewed the literature on cancer and propolis, addressing experimental carcinogenesis, chemically induced by 9,10-dimethyl-1,2-benzanthracene (DMBA), the chemical composition of propolis and its chemopreventive effects. The aim of the research paper was to evaluate the?tissue response?of tongue mucosa of hamsters, subjected to daily and alternating application of ethanolic extract of propolis (EEP) of a commercial presentation. Materials and methods: 60 hamsters were divided into three groups, and two experimental periods, 13 and 20 weeks. The lateral edge of the tongue underwent daily and alternate topical application of DMBA 0.5% and 30% EEP (EEP group, n = 20), DMBA and 0.5% aqueous extract of propolis (EAP) (EAP group; n = 20), and DMBA 0.5%?saline (DMBA?group, n =?20). The occurrence of types of clinical and histological changes was analysed. The area and volume of clinical changes, the occurrence of structural (AE) and cytological (CA) changes of squamous epithelial tissue with atypia and the histological area of squamous cell carcinoma (SCC) were measured. For statistical analysis, ANOVA, chi-square and Kruskal-Wallis and Mann-Whitney test were applied. Results: For all variables, there were no significant differences when comparing the groups in the two experimental periods. In 13 weeks, a single lesion of SCC in EEP group was observed. At 20 weeks, the highest occurrence of SCC was also in the group EEP. Conclusion: the EEP, with an alcohol content of 30% favored tissue reaction of initiation and promotion of carcinogenesis by mechanisms not yet elucidated.
| Identifer | oai:union.ndltd.org:IBICT/oai:acervo.ufvjm.edu.br/jspui:1/463 |
| Date | 10 February 2012 |
| Creators | Rocha, Ricardo Lopes |
| Contributors | Verli, Flaviana Dornela, Aguiar, Maria C?ssia Ferreira de, Gon?alves, Patr?cia Furtado, Lima, N?dia Lages, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Verli, Flaviana Dornela |
| Publisher | UFVJM |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFVJM, instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri, instacron:UFVJM |
| Rights | A concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data., info:eu-repo/semantics/openAccess |
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