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Previous issue date: 2015-07-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Os produtos naturais derivados de plantas servem de mat?ria-prima para a s?ntese
de fitoter?picos, sendo amplamente utilizados por v?rias popula??es em todo o
mundo. Dentre estes compostos, destacam-se as saponinas e seus metab?litos.
Seu intenso uso ? decorrente de suas propriedades bioativas, somado a sua
similaridade estrutural a horm?nios. As saponinas derivam-se em sapogeninas,
como, por exemplo, a Diosgenina e a Hecogenina. Al?m de serem utilizadas na
ind?stria farmac?utica para a produ??o de horm?nios e anti-inflamat?rios
esteroidais, estas duas sapogeninas tamb?m s?o empregadas como fitoter?picos,
estando presentes tamb?m em vegetais que comp?em a dieta de muitas
popula??es. Apesar da vasta utiliza??o destes compostos, existe uma falta de
informa??es sobre a toxicidade gen?tica, o que torna o consumo destas
sapogeninas um risco para as popula??es. Diante deste contexto, o presente
trabalho apresentou como objetivo investigar as potencialidades t?xicas, em n?vel
celular e de DNA, da Diosgenina e da Hecogenina. Ensaios em c?lulas HepG2 para
a avalia??o da citotoxicidade, genotoxicidade, e mutagenicidade foram realizados.
Considerando os resultados obtidos com a Diosgenina, esta sapogenina revelou
efeito citot?xico em concentra??es acima de 30 ?M, assim como induziu
significativamente incrementos na frequ?ncia de danos no DNA e de micron?cleos.
Por outro lado, a Hecogenina somente se revelou citot?xica acima de 150 ?M,
por?m em concentra??es abaixo deste valor, foi capaz de induzir danos ao material
gen?tico sem promover um aumento na frequ?ncia de micron?cleos. A an?lise do
conjunto destes dados sugere que o consumo de alimentos, fitoter?picos ou de
medicamentos que contenham a Diosgenina na sua composi??o apresenta um risco
maior de toxicidade, quando comparados com as formula??es a base de
Hecogenina, uma vez que a Diosgenina apresenta um potencial mutag?nico
demonstrado por meio de um aumento significativo na frequ?ncia de micron?cleos
que s?o biomarcadores de instabilidade gen?mica. / Natural products derived from plants are used as raw material for the synthesis of
phytotherapeutics, and are widely used by many populations around the world.
Among these compounds, there are the saponins and its metabolites. Its intense use
is due to its bioactive properties, added to its structural similarity to hormones.
Saponins derived in sapogenins, as Diosgenin and Hecogenin. Besides its use by
pharmaceutical industries to synthesis of steroidal hormones and anti-inflammatories,
these two sapogenins are also used as phytotherapeutics, being also present in
vegetables from diets of many populations. Despite the widespread use of these
compounds, there are a lack of information about their genetic toxicity, which makes
the use of these sapogenins a risk. Given this context, the present work aimed to
investigate the toxic potential of Diosgenin and Hecogenin, at cellular and DNA level.
Assays with HepG2 cells was performed to evaluate the cytotoxicity, genotoxicity and
mutagenicity. Considering the results obtained with Diosgenin, that sapogenin
showed cytotoxic effects at concentrations higher than 30 ?M, as well as significantly
induced increases in frequency of DNA damage and micronucleus. On the other
hand, Hecogenin only revealed cytotoxicity above 150 ?M, however, at
concentrations below this value, it was able to induce DNA damage without promote
an increase in micronucleus frequency. The analysis of these results together
suggests that the consumption of foods, phyotheraputics or medicines containing
Diosgenin on its composition, presents a higher risk of toxicity when compared with
formulations with Hecogenin, since Diosgenin shows a mutagenic potential
demonstrated by a significant increase in micronucleus frequency, which are
biomarkers of genome instability.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20332 |
| Date | 30 July 2015 |
| Creators | Cruz, Marina Sampaio de Menezes |
| Contributors | 91206359072, http://lattes.cnpq.br/4440806451383783, Sinigaglia, Marialva, 60901691020, http://lattes.cnpq.br/5450809564180533, Pedrosa, Matheus de Freitas Fernandes, 96728647449, http://lattes.cnpq.br/2929963416385218, Amaral, Viviane Souza do |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM BIOQU?MICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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