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Previous issue date: 2014-09-25 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Muitas esp?cies se especializaram em viver nos mais variados ambientes existentes demonstrando a not?vel capacidade de adapta??o do mundo microbiano as mais diversas condi??es f?sico-qu?micas. Ambientes expostos ? radia??o natural e a metais s?o escassos ao redor do mundo, apresentando uma microbiota ainda desconhecida. Com um n?mero total estimado entre 4 e 6 x 1030 microrganismos na terra, estes constituem um enorme pool biol?gico e gen?tico a ser explorado. Abordagens metagen?micas, independentes de cultivo, proporcionam uma nova forma de acesso ao potencial gen?mico de amostras ambientais tornando-se uma importante ferramenta para elucida??o de fun??es ecol?gicas, bem como para identifica??o de novas esp?cies e biomol?culas. Neste trabalho, o material gen?tico ambiental de amostras de solo e ?gua do A?ude Boqueir?o de Parelhas-RN, sob influ?ncia de radia??o natural e da presen?a de metais, foi extra?do, pirosequenciado, e as sequ?ncias geradas foram analisadas atrav?s de programas de bioinform?tica (MG-RAST e STAMP). Perfis taxon?micos comparativos de ambas as amostras mostraram alta abund?ncia do Dom?nio Bacteria, seguida por uma pequena parcela atribu?da aos Dom?nios Eucaryota, Archaea e V?rus. Proteobacteria, Actinobacteria e Bacterioidetes foram os filos que mostraram maior domin?ncia em ambas as amostras. Importantes g?neros e esp?cies associados ? resist?ncia aos agentes estressores encontrados na regi?o foram observados. Sequ?ncias relacionadas ? replica??o e reparo do DNA, ao estresse oxidativo e estresse pelo calor e a resist?ncia a compostos t?xicos foram observadas, mostrando uma importante rela??o entre a microbiota e seu perfil metab?lico, influenciada pelas vari?veis ambientais regionais. Os resultados encontrados neste estudo adicionam valiosos e in?ditos dados sobre a composi??o de comunidades microbianas nestas regi?es. / Many species have specialized to live in the most varied existing
environments showing the remarkable adaptability of the microbial world the
most diverse physicochemical conditions. Environments exposed to natural
radiation and metals are scarce around the world, presenting a microbiota still
unknown. With a total number estimated between 4 and 6 x 1030 microrganisms
on earth, they constitute an enormous biological and genetic pool to be
explored. Metagenomic approach independent of cultivation, provides a new
form to access to the potential genomic environmental samples becoming a
powerful tool for the elucidation of ecological functions, metabolic profiles, as
well as to identify new biomolecules. In this context, the genetic material of
environmental soil and water samples from A?ude Boqueirao Parelhas-RN,
under the influence of natural radiation and the presence of metals, was
extracted, pirosequencing and the generated sequences were analyzed by
bioinformatics programs (MG-RAST and STAMP). Taxonomic comparative
profiles of both samples showed high abundance of Domain Bacteria, followed
by a small portion attributable to Eucaryota Domains, Archaea and Viruses.
Proteobacteria, Actinobacteria and Bacterioidetes phyla showed the greater
dominance in both samples. Important genera and species associated with
resistance to various stressors found in region were observed. Sequences
related to oxidative and heat stress, DNA replication and repair, and resistance
to toxic compounds were observed, suggesting a significant relationship
between the microbiota and their metabolic profile, influenced by regional
environmental variables. The results of this study add valuable and unpublished
data on the composition of microbial communities in these regions
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/19511 |
| Date | 25 September 2014 |
| Creators | Ferreira, Henrique C?sar de Jesus |
| Contributors | 32398336468, http://lattes.cnpq.br/5882662534904226, Duarte, F?bio Teixeira, 03063680486, http://lattes.cnpq.br/4371235140013662, Agnez, Lucymara Fassarella, 00297997750, http://lattes.cnpq.br/1083882171718362, Medeiros, Silvia Regina Batistuzzo de |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM BIOQU?MICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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