Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-11-11T23:45:15Z
No. of bitstreams: 1
Disserta??o Soniane.pdf: 4275209 bytes, checksum: ccb64680a02e7e062aaddf493949e519 (MD5) / Made available in DSpace on 2015-11-11T23:45:15Z (GMT). No. of bitstreams: 1
Disserta??o Soniane.pdf: 4275209 bytes, checksum: ccb64680a02e7e062aaddf493949e519 (MD5)
Previous issue date: 2013-01-25 / The objectives of this study were to obtain and evaluate interspecific hybrids of the genus Psidiumto resistance to M. enterolobii and analyze the genetic variability of accessions of guava and ara?azeiros of the active germplasm bank of Embrapa Semi-Arid based on microsatellites, to guide genetic resources and breeding programs, emphasizing crosses of guava with other Psidium species.The crosses were performed when the calyx ruptured on the flower buds. When the plants reached15 to 20 cm tall, they were inoculated with a suspension containing 10,000 nematode eggs. Four months after inoculation, the soils were removed from the plants for individual evaluation of the number of galls, number of eggs and reproduction factor (RF). Genotyping was performed with two and 13 microsatellite loci in hybrids and Psidium accessions, respectively. Total DNA was extracted by the CTAB 2x method and PCR products were analyzed by denaturing polyacrylamide gel 6%, stained with silver nitrate. Interspecific hybrids between GUA 161 PE P guajava x ARA 138 RR P. guineensewere highly tolerant to nematodes, with growth similar to the guava, and demonstrated high compatibility when grafted with the cultivar 'Paluma'. The 61 accessionsPsidiumaccessions showed similarity coefficient ranging from 0.75 to 1.00 and the dendrogram presented co-phenetic value of 0.85. Genetic variability was considered high ?ST=0.238 and it was observed formation of five groups at a cutoff of 83% similarity. The genetic similarity among P.guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interespecific hybrids among these two species. / Os objetivos deste trabalho foram obter e avaliar h?bridos interespec?ficos do g?nero Psidium para resist?ncia ao M. enterolobii e analisar a variabilidade gen?tica de acessos de goiabeiras e ara?azeiros do banco ativo de germoplasma da Embrapa Semi?rido, com base em microssat?lites, para fornecer subs?dios para programas de recursos gen?ticos e de melhoramento com ?nfase para cruzamentos de goiabeira com outras esp?cies do g?nero Psidium.Os cruzamentos foram realizados quando os bot?es florais apresentavam ruptura do c?lice.Quando as plantas atingiram 15 a 20 cm de altura, foi realizada a inocula??o com suspens?o contendo 10.000 ovos do nematoide. Quatro meses ap?s a inocula??o, foi removido o solo das plantas para avalia??o individual para n?mero de galhas, n?mero de ovos e fator de reprodu??o (FR). A genotipagem foi feita com dois e 13 locos de microssat?lites, nos h?bridos e nos acessos de Psidium. O DNA total foi extra?do pelo m?todo CTAB 2x e os produtos de PCR foram analisados em gel de poliacrilamida desnaturante 6% e corados com nitrato de prata. H?bridos interespec?ficos entre GUA 161 PE de P. guajava x ARA 138 RR de P. guineense foram altamente resistentes ao nematoide, apresentando crescimento similar ao da goiabeira, e demonstrado alta compatibilidade quando enxertada com a cultivar ?Paluma?.Os 61 acessos de Psidium apresentaram coeficiente de similaridade variando de 0,75 a 1,00. O dendrograma apresentou valor co-fen?tico de 0,85. A variabilidade gen?tica foi considerada alta 0,238(?ST)e observou-se forma??o de cinco grupos no ponto de corte de 83% de similaridade.A similaridade gen?tica entre P. guineense e alguns acessos de goiabeira foi superior a 80%, indicando maior possibilidade de sucesso em cruzamentos interespec?ficos entre essas duas esp?cies.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/250 |
| Date | 25 January 2013 |
| Creators | Costa, Soniane Rodrigues da |
| Contributors | Santos, Carlos Antonio Fernandes |
| Publisher | Universidade Estadual de Feira de Santana, Mestrado Acad?mico em Recursos Gen?ticos Vegetais, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2441563959449389152, 600, 600, 600, -6971480722008537872, -1634559385931244697 |
Page generated in 0.0025 seconds