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Previous issue date: 2016-07-27 / Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do RJ - FAPERJ / This work was divided in four chapters, in which the first was performed with the goal of
quantifying the condensed tannin (CT) content by the Stiasny?s reaction and to determine the
classes of secondary metabolites present by the phytochemical prospection technique and
magnetic resonance in the tropical forage legumes Cajanus cajan (guandu - GUA), Gliricidia
sepium (gliricidia - GLI), Flemingia macrophylla (flemingia - FLE), Cratylia arg?ntea
(cratilia - CRA), Mimosa caesalpineafolia (sabi?) (this legume divided into bark and leaf
fraction ? SABc and SABf) among the treatments. The extracts obtained were divided into:
total extract, number of Stiasny (NS), CT and non-tannins. The FLE, CRA, GUA,GLI, SABf
and SABc had obtained the following values for total extract: 13.20; 13.06; 8.28; 14.73; 15.67
and 6.22%, respectively. The reactivity by NS, in the same order of legumes, was 11.25; 4.54;
7.37; 6.70; 23.06 and 71.62%, whereas the CT presented the following values: 1.52; 0.59;
0.61; 0.96; 3.6 and 4.43%, and non-tannin was 11.68; 12.46; 7.67; 13.75; 12.07 and 1.76%,
respectively. The following classes of secondary metabolites were identified with greater
evidence: saccharides, carbohydrates, non-protein amino acids and glicos?deos cardioativos.
For the CT, the intensity was low for most of the legumes, with greater content in CRA, GUA
and SABf. The wain compound in the extracts was methyl-inositol (sugar). The second
chapter had the objective of assaying in the legumes mentioned above and one more specie,
Stylosanthes spp. (estilosantes-EST), condensed tannin (CT) constituents, with the use of
organic solvents, soluble CT (ECT), CT adhered to protein (PBCT), CT adhered to fiber
(FBCT), and total CT (TCT), CT structural pro-pelargonidin (PP); prodelfinidin (PD) and
procyanidin (PC), molecular weight (polymerization degree (DP), molecular distance
distributed of the polymer (PDI); average weight of molecular mass (Mw), and average
number of molecular mass (Mn), and the biological activity through precipitated proteins by
phenols (PPP). The variables ECT, PBCT, and TCT presented were influenced by different
species (P?0.05). The FBCT fraction was not found in the legumes. Molecular weights (DP,
PDI, Mw e Mn) were affected by the different species (P?0.05), ranging from 737 to 1168 da.
The structural characteristics (PP, PD, PC and PD:PC) varied among the species. In the third
chapter I evaluated methanogenesis (total methane (CH4total)), incubated (CH4inc) and
fermented (CH4ferm) and ruminal fermentation parameters total gas production (PGT), pH,
ammonium (N-NH3), short-chain fatty acids (SCFA) and in vitro organic matter
disappearance (IVOMD) as they related to CT present in the legumes and Urochloa brizantha
cv. marandu hay as control (CTL). The effect of polyethylene glycol (PEG) on the leaf
fraction of sabi? was tested as well, which had a CT content of 15.97%. No alteration in the
pH (P?0.05) for the treatments evaluated. However, a decrease of total gas and methane
production for all the treatments with presence of CT (P?0.05). When PEG was added, there
was a 27.01 (8% PEG) and 35.01 (16% PEG) increase in total gas production and 3.59 (8%
PEG) and 4.15 (16% PEG) of methane production. GUA, FLE, SABc and SABf were capable
of modifying (P?0.05) the content of NH3-N (mg/dL), along with the CTL, which also
presented lower values compared to legumes with no or only traces of CT (ETL, CRA and
GLI). There was significant difference (P?0.05) for IVOMD between the legumes and
control, it was observed lower disappearance (P?0.05) for FLE, GUA and SABf in relation to
CTL, while SABc did disappear. The SABf IVOMD was affected by the addiction of PEG.
There was lower digestibility for FLE, GUA and SABf in relation to the CTL, while the bark
fraction of SAB did not disappear at all. On the SCFA profile, there was difference (P?0.05)
among the treatments evaluated, with lower values for the legumes with presence of CT. In
the fourth chapter I tested the effect of CT from the legumes in study (FLE, CRA, GUA,
GLI, EST, SABf and SABc) on larval migration inhibition (LMI) in vitro, on the infective
larvae L3 of the nematode Haemonchus contortus (HC), compared with Ivermectin and a
negative control (rumen fluid and buffer). Among the legumes studied, SABf and GUA did
not differ (P?0.05), with the greater (P?0.05) LMI percentage (34.75% and 34.33%) than the
other entries. The legumes GUA, FLE and SABc did not differ (P?0.05), presenting moderate
values of LMI (30.25%, 30.0% and 29.75%, respectively). Among the legumes studied, the
lowest LMI percentage was CRA (18.46%), GLI (23.75%) and negative control (rumen fluid
and buffer), with values near (P?0.05) from to Ivermectin (22.0%). / Este trabalho foi dividido em quatro cap?tulos. O primeiro realizou-se com o objetivo de
quantificar o teor de tanino condensado (TC) atrav?s da Rea??o de Stiasny e conhecer as
classes de metab?litos secund?rios presentes pela t?cnica de prospec??o fitoqu?mica e
resson?ncia magn?tica nas leguminosas forrageiras tropicais Cajanus cajan (guandu-GUA),
Gliricidia sepium (gliricidia-GLI), Flemingia macrophylla (flemingia-FLE), Cratylia
arg?ntea (cratilia-CRA), Mimosa caesalpineafolia (sabi?) sendo que essa leguminosa tinha a
fra??o casca e folha (SABc e SABf) entre os tratamentos. Os extratos obtidos foram divididos
em: extrato total, n?mero de Stiasny (NS), TC e n?o taninos. A FLE, CRA, GUA, GLI, SABf
e SABc apresentaram os valores para o extrato total 13,20; 13,06; 8,28; 14,73; 15,67 e 6,22%,
respectivamente. A reatividade pelo NS, na mesma ordem das leguminosas, foi de 11,25;
4,54; 7,37; 6,70; 23,06 e 71,62%, j? o TC apresentou os seguintes valores 1,52; 0,59; 0,61;
0,96; 3,6 e 4,43% e o n?o tanino foi de 11,68; 12,46; 7,67; 13,75; 12,07 e 1,76%,
respectivamente. Foram identificadas as seguintes classes de compostos secund?rios em
maiores evid?ncias: os sacar?deos, carboidratos, amino?cidos n?o prot?icos e os glicos?deos
cardioativos. J? para o TC, a intensidade foi baixa para grande parte das leguminosas,
prevalecendo maior teor para CRA, GUA e SABf. Foi constatado como componente principal
nos extratos o metil-inositol (a??car). O segundo cap?tulo teve como objetivo avaliar nas
leguminosas citadas acima e mais uma esp?cie, o Stylosanthes spp (estilosantes-EST), analisar
os constituintes do TC com uso de solvente org?nico, tanino sol?vel (TCE), tanino aderido ?
prote?na (TCPB), tanino aderido ? fibra (TCFB) e taninos condensados totais (TCT),
caracter?sticas estruturais tais como: propelargonidina (PP); prodelfinidina (PD) e
procianidina (PC); peso molecular (grau de polimeriza??o?(DP); dist?ncia do peso molecular
distribu?do do pol?mero (PDI); peso m?dio da massa molecular (Mw); n?mero m?dio da
massa molecular (Mn); al?m de determinar a atividade biol?gica, atrav?s da t?cnica de
prote?nas precipit?veis por fen?is (PPP). As vari?veis TCE, TCPB e TCT apresentadas foram
influenciadas pelas diferentes esp?cies (P?0,05). A fra??o TCFB n?o foi constatada nas
leguminosas. Os pesos moleculares (Mw) foram influenciados pelas diferentes esp?cies
(P?0,05), variando de 737 a 1168 Da. As caracter?sticas estruturais (PP, PD, PC e PD:PC)
tiveram varia??o entre as esp?cies estudadas. Objetivou-se com o terceiro cap?tulo avaliar a
metanog?nese (metano total (CH4-total), incubado (CH4 inc.) e fermentado (CH4 ferm.) e os
par?metros de fermenta??o ruminal (produ??o de g?s total (PGT), pH, am?nia (N-NH3),
?cidos graxos de cadeia curta (AGCC) e digestibilidade in vitro da mat?ria org?nica
(DIVMO) frente aos TC presentes nas leguminosas e feno de Urochloa brizantha cv.
marandu como controle (CTL). Foi testado tamb?m o efeito do polietileno glicol (PEG) sobre
a fra??o folha do sabi?, que teve conte?do de TC de 15,97%. N?o foi observado altera??o no
pH (P?0,05) para os tratamentos avaliados. No entanto, foram observadas diminui??o da
produ??o total de g?s e produ??o de metano para todos os tratamentos com presen?a de TC
(P?0,05). Para o tratamento com PEG houve aumento de 27,01 (8% PEG) e 35,01 (16% PEG)
na produ??o total de g?s e 3,59 (8% PEG) e 4,15 (16% PEG) na produ??o de metano. GUA,
FLE, SABc e SABf foram capazes de modificar (P?0,05) a concentra??o de N-NH3 (mg/dL)
juntamente com o CTL, que tamb?m apresentou valores inferiores comparado as leguminosas
com tra?os e aus?ncia do TC (ETL, CRA e GLI). Houve diferen?a (P?0,05) para DIVMO
entre as leguminosas e o controle, observou-se menor digestibilidade (P?0,05) para FLE,
GUA e SABf, em rela??o ao CTL, n?o sendo digest?vel o SABc. A DIVMO foi afetada pela
adi??o de PEG na dieta do SABf. No perfil dos AGCC houve diferen?a (P?0,05) para os
tratamentos avaliados, com menor valor para as leguminosas com presen?a de TC. O quarto
cap?tulo teve como objetivo testar o efeito da t?cnica de inibi??o da migra??o larval (IML) in
vitro do TC proveniente das leguminosas em estudo (FLE, CRA, GUA, GLI, EST, SABf e
SABc) sobre as larvas infectantes L3 do nemat?de o Haemonchus contortus (HC)
comparando com Ivermectina e controle negativo (l?quido ruminal e tamp?o). Entre as
leguminosas estudadas o SABf e GUA n?o diferiram entre si (P?0,05), com as maiores
porcentagens IML (34,75% e 34,33%). As leguminosas GUA, FLE e SABc n?o diferiram
entre si (P?0,05), apresentando moderados valores de IML (30,25%, 30,0% e 29,75%,
respectivamente). Entre as leguminosas estudadas a menor porcentagem de IML foi para CRA
(18,46%), GLI (23,75%) e controle negativo (l?quido de r?men e tamp?o) valores pr?ximos
do controle positivo com Ivermectina (22,0%).
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2102 |
| Date | 27 July 2016 |
| Creators | Pereira, Tatiana Pires |
| Contributors | Modesto, Elisa Cristina, Carvalho, Mario Geraldo de, Muir, James Pierre, Ferreira, Evandro Maia, Almeida, Jo?o Carlos de Carvalho, Nepomuceno, Delci de Deus |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Zootecnia, UFRRJ, Brasil, Instituto de Zootecnia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | AERTS, R.J.; BARRY, T.N., MCNABB, W.C. Polyphenols and agriculture: beneficial effects of proanthocyanidins in forages. Agriculture, Ecosystems and Environment, v.75, p. 1-2, 1999. ADEMOLA, I.O.; ELOFF, J.N. In vitro anthelmintic activity of Combretum molle (R. Br. ex G. Don) (Cunbretaceae) against Haemonchus contortus ova and larvae. Veterinary Parasitology, v. 169, p. 198-203, 2010. ADEMOLA, I.O.; IDOWU, S.O. Anthelmintic activity of Leucaena leucocephala seed extract on Haemonchus contortus infective larvae. Veterinary Record. v.158, p.485-486, 2006. ADEMOLA, I.O.; FAGBEMI, B.O.; IDOWU, S.O. Anthelmintic activity of extracts of Spon-dias mombin against gastrointestinal nematodes of sheep: studies in vitro and in vivo. Tropical Animal Health and Production, v.37, p.223-235, 2005. ALONSO-D?AZ, M.A.; TORRES-ACOSTA, J.F.J.; SANDOVALCASTRO, C.A.; HOSTE, H. Comparing the sensitivity of two in vitro assays to evaluate the anthelmintic activity of tropical tannin rich plant extracts against Haemonchus contortus. Veterinary Parasitology, v.181, p. 360-364, 2011. ALONSO-D?AZ, M.A.; TORRES-ACOSTA, J.F.J.; SANDOVAL-CASTRO, C.A.; AGUILAR CABALLERO, A.J.; HOSTE, H. In vitro larval migration and kinetics of exsheathment of Haemonchus contortus exposed to four tropical tanniniferous plant extracts. Veterinary Parasitology, v.153, p.313-319, 2008a. ALONSO-D?AZ, M.A.; TORRES-ACOSTA, J.F.J.; SANDOVAL-CASTRO, C.A.; CAPETILLO-LEAL, C.M.; BRUNET, S.; HOSTE, H. Effects of four tropical tanniniferous plant extracts on the inhibition of larval migration and the exsheathment process of Trichostrongylus colubri- formis infective stage. Veterinary Parasitology, v.153, p.187-192, 2008b. ?LVAREZ DEL PINO, M.C.; HERV?S, G.; MANTEC?N, A.R.; GIR?LDEZ F.J.; FRUTOS, P. Comparison of biological and chemical methods, and internal and external standards, for assaying tannins in shrub species. Journal of the Science of Food and Agriculture, v.85, p.583-590, 2005. ANANTASOOK, N.; WANAPAT, M.; CHERDTHONG, A.; GUNUN, P. Effect of tannins and saponins in Samaneasaman on rumen environment, milk yield and milk composition in lactating dairy cows. Journal of animal physiology and animal nutrition, v.99, p.335-344, 2014. AOAC. 1990. Official Methods of Analysis of the Association of Official Analytical Chemists. Washington, Association of Official Analytical Chemistsa, 15th ed., p.369-406. ARCHIM?DE, H.; EUG?NE, M.; MARIE MAGDELEINE, C.; BOVAL, M.; MARTIN, C.; MORGAVI, D.P.; LECOMTE, P.; DOREAU, M. Comparison of methane production 62 between C3 and C4 grasses and legumes. Animal Feed Science and Technology, v.166, p.59-64, 2011. ARMSTRONG, S.A.; KLEIN, D.R.; WHITNEY, T.R.; SCOTT, C.B.; MUIR, J.P.; LAMBERT, B.D.; CRAIG, T.M. Effect of using redberry juniper (Juniperus pinchotii) to reduce Haemonchus contortus in vitro motility and increase ivermectin efficacy. Veterinary Parasitology, v.197, p. 271-276, 2013. ATHANASIADOU, S.; KYRIAZAKIS, I.; JACKSON, F.; COOP, R.L. Direct anthelmintic effects of condensed tannins towards different gastrointestinal nematodes of sheep: in vitro and in vivo studies. Veterinary Parasitology, v.99, p. 205-219, 2001. BAE, H.D.; McALLISTER, T.A.; YANKE, J. Effects of condensed tannins on endoglucanase activity and filter paper digestion by Fibrobacter succinogenes S85. Applied and Environmental Microbiology, v.59, p. 2132-2138, 1993. BALOGUN, R.O.; JONES, R.J.; HOLMES, J.H.G. Digestibility of some tropical browse species varying in tannin content. Animal Feed Science and Technology, v.76, p.77-88, 1998. BARRAU, E.; FABRE, N.; FOURASTE, I.; HOSTE, H. Effect of bioactive compounds from Sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. Parasitology, v. 13, p. 531-538, 2005. BARRY, T. N.; McNABB, W.C. The implications of condensed tannins on the nutritive value of temperateforages fed to ruminants. British Journal of Nutrition, v. 81, p. 263-272, 1999. BARRY, T.N.; MANLEY, T.R.; DUNCAN, S.J. The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep: IV. Sites of carbohydrate and protein digestion as influenced by dietary reactive tannin concentration. British Journal of Nutrition, v.55, p.137, 1986. BATE-SMITH, E.C. Hemanalysis of tannin-Concept of relative astringency. Phytochemistry, v.12, p.907-912, 1973. BEN SALEM, H.A.; NEFZAOUI, L.; BEN SALEM, A.; TISSER, J.L. Deactivation of condensed tannins in Acacia cyanophylla Lindl. Foliage by polyethylene glycol in feed blocks effect on feed intake, diet digestibility, nitrogen balance, microbial synthesis and growth by sheep. Livestock Production Science, v.64, p.51-60, 2000. BEN-SALEM, H.; NEFZAOUI, A.; BEN-SALEM, L.; TISSERAND, J.L. Different means of administering polyethylene glycol to sheep: effect on the nutritive value of Acacia cyanophylla Lindl. Foliage Animal Science, v.68, p.809-818, 1999. BHATTA, R.; SARAVANAN, M.; BARUAH, L.; SAMPATH, K.T.; PRASAD, C.S. Effect of plant secondary compounds on in vitro methane, ammonia production and ruminal protozoa population. Journal of Applied Microbiology, v.115, p.455-465, 2013. 63 BHATTA, R.; SHINDE, A.K.; VERMA, D.L.; SANKHYAN, S.K.; VAITHIYANATHAN, S. Effect of supplementation containing polyethylene glycol (peg)-6000 on intake, rumen fermentation pattern and growth in kids fed foliage of Prosopis cineraria. Small Ruminant Research, v. 52, p. 45-52, 2004. BHATTA, R.; SHINDE, A.K.; VAITHIYANATHAN, S.; SANKHYAN, S.K.; VERMA, D.L. Effect of polyethylene glycol-6000 on nutrient intake, digestion and growth of kids browsing prosopis cineraria. Animal Feed Science and Technology, v.101, p.45-54, 2002. BHATTA, R.; KRISHNAMOORTHY, U.; MOHAMMED, F. Effect of tamarind (Tamarindus indica) seed husk tannins on in vitro rumen fermentation. Animal Feed Science and Technology, v.90, p.143-152, 2001. BRAZ-FILHO, R. Contribui??o da fitoqu?mica para o desenvolvimento de um pa?s emergente. Qu?mica Nova, v.33, p. 229-239, 2010. BRITO, C. J. F. A.; RODELLA, R. A.; DESCHAMPS, F.C. Perfil qu?mico da parede celular e suas implica??es na digestibilidade de Brachiaria brizantha e Brachiaria humidicola. Revista Brasileira de Zootecnia, v.32, p.1835-1844, 2003. BRODERICK, G.A.; KANG, J.H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, v.63, p.64- 75, 1980. BRUNET, S.; HOSTE, H. Monomers of condensed tannins affect the larval exsheathment of parasitic nematodes of ruminats. Journal of Agricultural and Food Chemistry, v. 54, p. 7481-7487, 2006. BUTTER, N. L.; DAWSON, J. M.; WAKELIN, D.; BUTTERY, P.J. Effect of dietary tannin and protein concentration on nematode infection (Trichostrongylus colubriformis) in lambs. Journal of Agricultural Science, v. 134, p. 89-99, 2000. CALDERON-QUINTAL, J.A.; TORRES-ACOSTA, J.F.J.; SANDOVAL-CASTRO, C.A.; ALONSO- D?AZ, M.A.; HOSTE, H.; AGUILAR-CABALLERO, A. Adaptation of Haemonchus contortus to condensed tannins: can it be possible. Archivos de Medicina Veterinaria, v.42, p.165-171, 2010. CARNEIRO A.; BENEDITO B.; FREDERICO P.G.; CARVALHO A.M.; VIDAURRE G. Propriedades de chapas de aglomerado fabricadas com adesivo t?nico de angico-vermelho (Anadenanthera peregrina) e ureia-formade?do. Revista ?rvore, v. 33, p.521-531, 2009. CENCI, F.B.; LOUVANDINI, H.; McMANUS, C.M.; DELL?PORTO, A.; COSTA, D.M.; ARA?JO, S.C.; MINHO, A.P., ABDALLA, A.L. Effects of condensed tannin from Acacia mearnsii on sheep infected naturally gastrintestinal helminthes. Veterinary Parasitology, v.144, p.132-137, 2007. CERRI, C. C.; BERNOUX, M.; MAIA, S.M.F. Greenhouse gas mitigations in Brazil for landuse change, livestock and agriculture. Scientia Agricola, v. 67, p.102-116, 2010. 64 CHIQUETTE, J.; CHENG, K.J.; COSTERTON, J.W.; MILLIGAN, L.P. Effect of tannins on the digestibility of two isosynthetic strains of birdsfoot trefoil (Lotus corniculatus L.) using in vitro and in sacco techniques. Canadian Journal of Animal Science, v.68, p.751-760, 1988. COSTA, B.M. DA, SANTOS, I.C.V., OLIVEIRA, G.J.C., PEREIRA, I.G. Avalia??o de folhas de Gliricidia sepium (Jacq.) Walp por ovinos. Archivos de Zootecnia, v.58, p. 33-41, 2009. DALTO, A.G.C.; BANDARRA, P.M.; PEDROSO, P.M.O.; GUAGNINI, F.S.; LEAL, J.S.; RAYMUNDO, D.L.; DRIEMEIER, D. Timpanismo espumoso em bovinos leiteiros em pastagens de Trifolium spp. (Leg. Caesalpinoideae). Pesquisa Veterin?ria Brasileira, v.29, p.401-403, 2009. DECANDIA, M.; SITZIA, M.; CABIDDU, A.; KABABYA, D.; MOLLE, G. The use of polyethylene glicol to reduce the anti-nutritional effects of tannins in goats fed woody species. Small Ruminant Research, v.38, p.157-164, 2000. FAGUNDES, G. M. Desempenho produtivo e composi??o do leite de cabras alimentadas com dietas contendo diferentes n?veis de Flemingia macrophylla (willd.) Merrill com e sem polietilenoglicol. 2012. 103f. Disserta??o (Mestrado em Produ??o Animal) Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2012. FALKENBERG, M. B.; SANTOS, R. I.; SIM?ES, C.M.O. Introdu??o ? an?lise fitoqu?mica. In: SIM?ES, C.M.O.; SCHENKEL, E.P.; GOSMANN, G.; MELLO, J.C.P.; MENTZ, L.A.; PETROVICK, P.R. Farmacognosia: da planta ao medicamento. Editora UFRGS, Porto Alegre, Editora UFSC, Florian?polis, 2004. FERREIRA, E. S. Utiliza??o dos polifen?is da casca de pinus para produ??o de adesivos para compensados. 79 f. Disserta??o (Mestrado em Ci?ncias Ambientais e Florestais) ? ?rea de Concentra??o em Tecnologia e Utiliza??o de Produtos Florestais da Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2004. FORKNER, R.E.; MARQUIS, R.; TILL, J.L. Condensed tannins as anti- herbivore defenses in leaf-chewing herbivore communities of Quercus. Ecological Entomology, v.29, p.174- 187, 2004. FRUTOS, P.; HERV?S, G.; RAMOS, G.; GIR?LDEZ, F. J.; MANTEC?N, A.R. Condensed tannin content of several shrub species from a mountain area in northern Spain, and its relationship to various indicators of nutritive value. Animal Feed Science and Technology, v. 95, p. 215-226, 2002. GEA, A.; STRINGANO, E.; BROWN, R.H.; MUELLER-HARVEY, I. In situ analysis and structural elucidation of sainfoin (Onobrychis viciifolia) tannins for high throughput germplasm screening. Journal of Agricultural and Food Chemistry, v.59, p.495?503, 2011. GETACGEW, G.; MAKKAR, H.P.S.; BECKER, K. Effect of polyethylene glycol on in vitro degradability of nitrogen and microbial protein synthesis from tannin-rich browse and herbaceous legumes. British Journal of Nutrition, v.84, p.73-83, 2000. 65 GETACHEW, G.; ROBINSON, P.H.; DEPETERS, E.J.; TAYLOR, S.J.; GISI, D.D.; HIGGINBOTHAM, G.E.; RIORDAN, T.J. Methane production from commercial dairy rations estimated using an in vitro gas technique. Animal Feed Science and Technology, v.124, p.391?402, 2005. GON?ALVES, C. A.; LELIS, R. C. C.; BRITO, E. O.; NASCIMENTO, A.M. Produ??o de chapas de madeira aglomerada com adesivo ur?ia-formalde?do modificado com tanino de Mimosa caesalpiniaefolia Benth (sabi?), Floresta e Ambiente, v. 10, p.18-26, 2003. GRAMINHA, C.V.; MARTINS, A.L.M.; FAI?O, C.A. Viabilidade de alguns aditivos utilizados no confinamento no Brasil. In: CONFINAMENTO: GEST?O T?CNICA E ECON?MICA, I, 2007, Jaboticabal. Anais... Jaboticabal, 2007, v.1, p.103-132. GRAINGER, C.; CLARKE, T.; AULDIST, M.J.; BEAUCHEMIN, K.A.; MCGINN, S.M.; WAGHORN, G.C.; ECKARD, R.J. Potential use of Acacia mearnsii condensed tannins to reduce methane emissions and nitrogen excretion from grazing dairy cows. Journal of Animal Science, v.89, p.241-251, 2009. GRIFFITHS, D.W.; BIRCH, A.N.E.; HILLMAN, J.R. Antinutritional compounds in the Brassicaceae: analysis, biosynthesis, chemistry and dietary effects. Journal of Horticultural Science and Biotechnology, v. 73, p. 1-18, 1998. GUIMAR?ES-BEELEN, P. M.; BERCHIELLI, T. T.; BUDDINGTON, R.; BEELEN, R. Efeito dos taninos condensados de forrageiras nativas do semi-?rido nordestino sobre o crescimento e atividade celulol?tica de Ruminococcus flavefaciens FD1. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, v. 58, p. 910-917, 2006. HAGERMAN, A.E.; BUTLER, L.G. Protein precipitation method for the quantitative determination of tannins. Journal of Agricultural and Food Chemistry, v. 26, p.809-812, 1978. HAMMOND, J.A.; FIELDING, D.; BISHOP, S.C. Prospects for plant anthelmintics in tropical veterinary medicine. Veterinary Research Communications, v.21, p.213-228, 1997. HECKENDORN, F.; H?RING, D.A.; MAURER, V.; SENN, M.; HERTZBERG, H. Individual administration of three tanniferous forage plants to lambs artificially infected with Haemonchus contortus and Cooperia curticei. Veterinary Parasitology, v.146, p.123-34, 2007. HERN-ANDEZ-ORDUNO, G.; TORRES-ACOSTA, J.F.J.; SANDOVAL-CASTRO, C.; AGUILAR-CABALLERO, A.J.; REYES-RAM?REZ, R.R.; HOSTE, H.; CALDERONQUINTAL, J.A. In vitro anthelmintic effect of Acacia gaumeri, Havardia albicans and Quebracho tannin extracts on a Mexican strain of Haemonchus contortus L3 larvae. Tropical and Subtropical Agroecosystems, v.8, p.191-197, 2008. HESS, H.D.; MONSALVE, L.M.; LASCANO, C.E.; CARULLA, J.E.; DIAZ, T.E.; KREUZER, M. Supplementation of a tropical grass diet with forage legumes and Sapindus saponaria fruits: effects on in vitro ruminal nitrogen turnover and methanogenesis. Australian Journal of Agricultural Research, v.54, p.703-713, 2003. 66 HIXSON, J.L.; BINDON, K.A.; SMITH, P.A. Evaluation of Direct Phloroglucinolysis and Colorimetric Depolymerization Assays and Their Applicability for Determining Condensed Tannins in Grape Marc. Journal of Agricultural and Food Chemistry, v.63, p. 9954?9962, 2015. HOONG, Y.B.; PARIDAHA, M.T.; LUQMANB, C.A.; KOHC, M.P.; LOH, Y.F. Fortification of sulfited tannin from the bark of Acacia mangium with phenol?formaldehyde for use as plywood adhesive. Industrial Crops and Products. v.30, p.416-421, 2009. HOSTE, H. Adaptive physiological processes in the host during gastrointestinal parasitism. Int. Journal of Parasitology. v.31, p.231-244, 2001. HOSTE, H.; JACKSON, F.; ATHANASIADOU, S.; THAMSBORG, S.M.; HOSKIN, S. O. The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends in Parasitology, v. 22, n. 6, p. 253- 261, 2006. HOLMES, P.H. Pathophysiology of nematode infections. University of Glasgow Veterinary Scholl, Bearsden Road, Glasgow, G61 (Scotland) U.K. p.443-451, 1987. HUANG, X.D.; LIANG, J.B.; TAN, H.Y.; YAHYA, R.; KHAMSEEKHIEW, B.; HO, Y.W. Molecular weight and protein binding affinity of Leucaena condensed tannins and their effects on in vitro fermentation parameters. Animal Feed Science and Technology, v.159, p.81-87, 2010. JOHNSON, K. A.; JOHNSON, D.E. Methane emissions from cattle. Journal of Animal Science, v. 73, p. 2483-2492, 1995. JONES, W.T.; BROADHURST, R.B.; LYTTLETON, J.W. The condensed tannins of pasture legume species. Phytochemistry, v.15, p.1407-1409, 1976. JONES, W.T.; MANGAN, J. L. Complexes of the condensed tannins of sainfoin (Onobrychis viciaefolia Scop) with fraction 1 leaf protein and with submaxillary mucoprotein and their reversal by PEG and pH. Journal of the Science of Food and Agriculture, v.28, p.126-136, 1977. KOTHARI, R.; SINGH, D.P.; TYAGI, V.V.; TYAGI, S.K. Fermentative hydrogen production ? An alternative clean energy source. Renewable and Sustainable Energy Reviews, v.16, p. 2337? 2346, 2012. KUNG, L.; BRACHT, J. P.; TAVARES J.Y. Effects of various compounds on in vitro ruminal fermentation and production of sulfide. Animal Feed Science and Technology, v.84, p.69?81. 2000. LASCANO, C.E.; C?RDENAS, E. Alternatives for methane emission mitigation in livestock systems. Revista Brasileira de Zootecnia, v.39, p.175-182, 2010. LELIS, R. C. C. Zur Bedeutung der Kerninhaltsstoffe obligatorisch verkernter Nadelbaumarten bei der Herstellung von feuchtebest?ndigen und biologisch resistenten Holzspanplatten, am Beispiel der Douglasie (Pseudotsuga menziesii Mirb. Franco). 175f. 67 Tese (Doutorado em Ci?ncias Florestais). Forstliche Fakult?t, Universit?t G?ttingen, G?ttingen, Alemanha, 1995. LEINMULLER, E.; MENKE, K.H. Tannine in Futtermittenln fur Wiederkauer. 1. Chemische Eigenschaften und Reaktionen mit Makromolekulen. Ubers Tierernahr, v.18, p.91?114, 1990. LIN, Y. M..; LIU, J. W.; XIANG, P.; LIN, P.; DING, Z. H.; STERNBERG, L.S. L. Tannins and nitrogen dynamics in mangrove leaves at different age and decay stages (Jiulong River Estuary,China). Hydrobiologia, v.583, p.285-295, 2007. LITTLEFIELD, K. A.; MUELLER, J. P.; MUIR J. P.; LAMBERT B. D. Correlation of Plant Condensed Tannin and Nitrogen Concentrations to White-Tailed Deer Browse Preferences in the Cross Timbers. Texas Journal of Agriculture and Natural Resources, v.24, p.1-7, 2011. LONGO, C.; HUMMEL, J.; LIEBICH, J.; BUENO, I.C.S. ; BURAUEL, P.; AMBROSANO, E.J.; BDALLA, A.L.; ANELE, U.Y. ; S?DEKUM, K.H. Chemical characterization and in vitro biological activity of four tropical legumes, Styzolobium aterrimum L., Styzolobium deeringianum, Leucaena leucocephala and Mimosa caesalpiniae folia, as compared to a tropical grass, Cynodon spp for the use in ruminant diets. Czech Journal of Animal Science, v.57, p.255-264, 2012. LUCIANO, G.;VASTA, V.; MONAHAN, F. J.; L?PEZ-ANDR?S, P.; BIONDI, L.; LANZA M.; PRIOLO, A. Antioxidant status, colour stability and myoglobin resistance to oxidation of longissimusdorsi muscle from lambs fed a tannin-containing diet. Food Chemistry. v. 124, p.1036-1042, 2011. MAKKAR, H.P.S.; BL?MMEL, M.; BECKER, K. In vitro effects and interactions of tannins and saponins and fate of tannins in rumen. Journal of the Science of Food and Agriculture, v.69, p.481-493, 1995. MAKKAR H.P.S. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin rich feeds, Small Ruminant Research, v.49, p.241-256, 2003. MAKKAR, H.P.S.; VERCOE, P.E. Measuring Methane Production from Ruminants. p.138, 2007. MANE, C.; SOMMERER, N.; YALCIN, T.; CHEYNIER, V.; COLE, R.B.; FULCRAND, H. Assessment of the molecular weight distribution of tannin fractions through MALDI-TOF MS analysis of protein-tannin complexes. Analytical Chemistry, v.79, p.2239-2248, 2007. MANSFIELD, J.L.; CURTIS, P.S.; ZAK, D.R.; PREGITZER, K.S. Genotypic variation for condensed tannin production in trembling aspen (Populus tremuloides, Salicaceae) under elevated CO2 and in high and low fertility soil. American Journal of Botany, v.86, p.1154- 1159, 1999. 68 MARIE-MAGDELEINE, C.; MAHIEU, M.; PHILIBERT, L.; DESPOIS, P.; ARCHIMEDE, H. Effect of cassava (Manihot esculenta) foliage on nutrition, parasite infection and growth of lambs. Small Ruminant Research, v.93, p.10-18, 2010. MARIE-MAGDELEINE, C.; UDINO, L.; PHILIBERT, L.; BOCAGE, B.; ARCHIMEDE, H. In vitro effects of Musa x paradisiaca extracts on four developmental stages of Haemonchus contortus. Research in Veterinary Science, v.96, p.127-132, 2014. MATOS, F.J.A. Introdu??o a fitoqu?mica experimental. Edi??es UFC, Fortaleza. 126p. 1988. MBUGUA, D.M.; PELL, A.N.; FOX, D.G.; SCHOFIELD, P. The effects of proanthocyanidins from Calliandra calothyrsus and the alkaloid sparteine on in vitro fiber digestion. Animal Feed Science and Technology, v. 121, p. 89-107, 2005. McALLISTER, T.A.; BAE, H.D.; JONES, G.A.; CHENG, K.J. Microbial attachment and feed digestion in the rumen. Journal Animal Science, v.72, p.3004?3018, 1994. McALLISTER T.A.; MARTINEZ T.; BAE H.D.; MUIR A.D.; YANKEL.J.; JONES G.A. Characterization of condensed tannins purified from legume forages: chromophore production, protein precipitation, and inhibitory effects on cellulose digestion. Journal of Chemical Ecology, v.31, p.2049-2063, 2005. McDOUGALL, E.I. The composistion and output of a sheep?s saliva. Biochemical Journal, v.43, p.99?109, 1947. McGEOUGH, E.J.; O'KIELY, P.; HART, K.J.; MOLONEY, A.P.; BOLAND, T.M; KENNY, D.A. ?Methane emissions, feed intake, performance, digestibility, and rumen fermentation of finishing beef cattle offered whole-crop wheat silages differing in grain content. Journal of Animal Science, v. 88, p. 2703-2716, 2010. McNABB, W.C.; WAGHORN, G.C.; BARRY, T.N.; SHELTON, I.D. The effect of condensed tannins in Lotus pedunculatus on the digestion and metabolism of methionine, cysteine and inorganic sulfur in sheep. British Journal of Nutrition. v. 70, p.647-661,1993. McSWEENEY, C. S.; PALMER, B.; BUNCH, R. Effect of the tropical forage calliandra on microbial protein synthesis and ecology in the rumen. Journal of Applied Microbiology, v. 90, p.78-88, 2001. MEALE, S. J.; MCALLISTER, T.A.; BEAUCHEMIN, K.A.; HARSTAD, O.M.; CHAVES, A.V. Strategies to reduce greenhouse gases from ruminant livestock. Acta Agricultura e Scandinavica, v. 62, p.199-211, 2012. MEZZOMO, R.; PAULINO, P.V.R.; DETMANN, E. Influence of condensed tannin on intake, digestibility and efficiency of protein utilization in beef steers fed high concentrate diet. Livestock Science, v. 141, p.1-11, 2011. MIN, B. R.; BARRY, T.N.; ATTWOOD, G.T.; McNABB, W.C. The effect of condensed tannins on thenutrition and health of ruminants fed fresh temperate forages: a review. Animal Feed Science and Technology, v. 106, p. 3-19, 2003. 69 MINHO, A.P.; BUENO, I. C. S.; GENNARI, S. M.; JACKSON, F.; ABDALLA, A. L. Effect of Acacia molissima tannin extract on the control of gastrointestinal parasites in sheep. Animal Feed Science and Technology, v.147, p.172-181, 2008. MINHO, A.P.; FILIPPSEN, L.F.; AMARANTE, A.F.T.; ABDALLA, A.L. Efficacy of condensed tannin present in acacia extract on the control of Trichostongylus colubriformis in sheep. Ci?ncia Rural, v. 40, p. 1360-1365, 2010. MOLE, S.; BUTLER, L.G.; IASON, G. Defense against tannin in herbivores: a survey for proline rich salivary proteins in mammals. Biochemical Systematics and Ecology, v.18, p.287-293, 1990. MOLAN, A.L.; DUNCAN, A.J.; BARRY, T.N.; MCNABB, W.C. Effect of condensed tannins and crude sesquiterpene lactones extracted from chicory on the motility of larvae of deer lungworms and gastrointestinal nematodes. Parasitology International, v.52, p.209- 218, 2003. MONTEIRO, J.E.B.A.; SENTELHAS, P.C.; CHIAVEGATO, E.J.; GUISELINI, C.; SANTIAGO, A.V.; PRELA, A. Estimula??o da ?rea foliar do algodoeiro por meio de dimens?es e massa das folhas. Bragantia, v.64, p. 15-24, 2005. MORAIS, J.A.S.; BERCHIELLI, T.T.; REIS, R.A. Aditivos. In: Berchielli, T.T.; Pires, A.V.; Oliveira, S.G. (Eds.), Nutri??o de Ruminantes. Funep, Jaboticabal, p. 583. 2006. MOREIRA, P.C.; MENDON?A, A.C.; MARTINS, A.P.; WASCHECK, R.C.; SOUZA, P.R.; DUTRA, A.R.; GRANDSIRE, C.; REZENDE, P.L.P.; CARDOSO, J.R.; BENETTI, E.J.; SILVA, M.S.B. Avalia??o do pH do fluido ruminal de vacas leiteiras. Estudos, Goi?nia, v. 36, p. 1201-1218, 2009. MOSS, A.R.; JOUANY, J.P.; NEWBOLD, J. Methane production by ruminants: its contribution to global warming. Annales de Zootechnie, v. 49, p. 231-253, 2000. MUELLER-HARVEY. Review. Unravelling the conundrum of tannins in animal nutrition and health. Journal of the Science of Food and Agriculture, v. 86, p.2010-2037, 2006. MUETZEL, S.; BECKER, K. Extractability and biological activity of tannins from various tree leaves determined by chemical and biological assays as affected by drying procedure. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 125, p. 139-149, 2006. MUIR, J. P. The multi-faceted role of condensed tannins in the goat ecosystem.Small Ruminant research, v.98, p. 115-120, 2011. MUPEYO, B.; BARRY, T.N.; POMROY, W.E.; RAM?REZ-RESTREPO, C.A.; L?PEZVILLALOBOS, N.; PERNTHANER, A. Effects of feeding willow (Salix spp.) upon death of established parasites and parasite fecundity. Animal Feed Science and Technology, v.164, p.8-20, 2011. NAUMANN, H. D.; TEDESCHI, L. O.; MUIR, J. P.; LAMBERT, B. D.; KOTHMANN, M. M. Effect of molecular weight of condensed tannins from warm-season perennial legumes on 70 ruminal methane production in vitro. Biochemical Systematics and Ecology, v.50, p.154- 162, 2013. NAUMANN, H.D.; ARMSTRONGC, S.A.; LAMBERT, B.D.; MUIR, J.P.; TEDESCHID, L.O.; KOTHMANN, M.M. .Effect of molecular weight and concentration of legume condensed tannins on in vitro larval Migration inhibition of Haemonchus contortus, Veterinary Parasitology, v.199, p.93- 98, 2014. NAUMANN, H.D.; HAGERMAN, A.E.; LAMBERT, B.D.; MUIR, J.P.; TEDESCHI, L.O.; KOTHMANN, M.M. Molecular weight and protein-precipitating ability of condensed tannins from warm-season perennial legumes. Journal of Plant Interactions, v.9, p.212-219, 2014. NAUMANN, H.D.; LAMBERT, B.D.; ARMSTRONG, S.A.; FONSECA, M.A.; TEDESCHI, L.O.; MUIR, J. P.; ELLERSIECK, M.R. Effect of replacing alfalfa with panicled-tick clover or sericea lespedeza in corn-alfalfa-based substrates on in vitro ruminal methane production. Journal of Dairy Science, v. 98, p.3980-3987, 2015. NEPOMUCENO, D.D.; ALMEIDA, J.C.C.; CARVALHO, M.G.; FERNANDES, R.D.; JUNIOR, F.E.A.C. Classes of secondary metabolites identified in three legume species. Revista Brasileira de Zootecnia, v.42, p.700-705, 2013. NOVOBILSK?, A.; STRINGANO, E.; HAYOT CARBONERO, C.; SMITH, L.M.J.; ENEMARK, H.L.; MUEL-LER-HARVEY, I.; THAMSBORG, S.M. In vitro effects of extracts and purified tannins of sainfoin (Onobrychis viciifolia) against cattle nematodes. Veterinary Parasitology, v.196, p.532-537, 2013. NOZELLA, E. F. Valor nutricional de esp?cies arb?reo-arbustivas nativas da caatinga e utiliza??o de tratamentos f?sicos-qu?micos para redu??o do teor de taninos. 2006. 99 f. Tese (Doutorado em Energia Nuclear na Agricultura) - Centro de Energia Nuclear na Agricultura, Universidade de S?o Paulo, Piracicaba, 2006. NOZELLA, E.F., 2001. Determina??o de taninos em plantas com potencial forrageiro para ruminantes. Centro de energia Nuclear na Agricultura. ESALQ, Piracicaba, p. 58. OLIVEIRA, S.G.; BERCHIELLI, T.T. Potencialidades da utiliza??o de taninos na conserva??o de forragens e nutri??o de ruminantes-revis?o, Archives of Veterinary Science, v.12, p.1-9, 2007. OLIVEIRA, S. G.; BERCHIELLI, T.T.; NATARELLI, B.; MALHEIROS, E.B. Valor aliment?cio e aspectos econ?micos de dietas com varia??o no teor de tanino e n?vel prot?ico em bovinos de corte. Revista Ceres, v. 55, p. 467-475, 2008. OLIVEIRA, M. V.; MOURA, M. S.; BARBOSA, F. C. Avalia??o comparativa do m?todo Famacha, volume globular e OPG em ovinos. PUBVET, v. 5, n. 7, Ed. 154, Art. 1039, 2011. OTERO, M.J.; HIDALGO, L.G. Taninos condensados en especies forrajeras de clima templado: efectos sobre productividad de rumiantes afectados por parasitosis gastrointestinales. Livestock Research for Rural Development, v.16, p.1-9, 2004. 71 PALMER, B.; MCSWEENEY, C.S. Tannins in Calliandra calothyrsus: effect of polyethylene glycol (PEG) and an evaluation of 19 accessions. In: Brooker, J.D. (Ed.), Tannins in ivestock and Human Nutrition. Proceedings of the International Workshop, Adelaide, Australia, May 31?June 2, 1999. ACIAR Proceedings, n. 92, p. 36?39, .2000. PATRA, A.K.; SAXENA, J. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. Journal of the Science of Food and Agriculture, v.91, p.24-37, 2011. PELLIKAAN, W.H.; HENDRIKS, G.; UWIMANA, L.J.G.M.; BONGERS, P.M.; BECKER, J.W. CONE. A novel method to determine methane production simultaneously during in vitro gas production measurements. Proc. 4th Greenhouse Gases Anim. Agric. p. 228, 2010. PEREIRA, L.G.R.; MACHADO, F.S.; CAMPOS, M.M. Avan?o conceitual em diagn?stico e estrat?gias de mitiga??o de metano ent?rico em bovinos de leite no Brasil. In: SIMP?SIO NACIONAL DE BOVINOCULTURA LEITEIRA, 3., Vi?osa, MG. Anais...Vi?osa, 2011. p. 75-122. PONCET, C.; R?MOND, D. Rumen digestion and intestinal nutrient flows in shepp consuming pea seeds: the effect of extrusion or chestnut tannin addition. Animal Research, v.51, p.201-216, 2002. PORDOMINGO, A. J.; VOLPI LAGRECA, G.; STEFANAZZI, I. N.; PORDOMINGO, A. B. Efecto de lainclusi?n de taninos versus monensina y de soja crudaen dietas basadasen grano entero, sin fibra larga en engorde de vaquillonas a corral. Bolet?n de Divulgaci?nT?cnica, EEA Anguil, n. 90, 2006. PORTER, L.J.; WOODRUFFE, J. Haemanalysis: The relative astringency of proanthocyanidin polymers. Phytochemistry, v.23, p.1255-1256, 1984. POSSENTI, R.A.; FRANZOLIN, R.; SCHAMMAS, E. A. Efeitos de dietas contendo Leucaena leucocephala e Saccharomyces cerevisiae sobre a fermenta??o ruminal e a emiss?o de g?s metano em bovinos. Revista Brasileira de Zootecnia, v.37, p.1509-1516, 2008. PRICHARD, R.K. Anthelmintic resistance.Veterinary Parasitology, v.54, p.259-268, 1994. PUCHALA, R.; MIN, R.B.; GOETSCH, A.L. The effect of a condensed tannin-containing forage on methane emission by goats. Journal of Animal Science, v.83, p.182-186, 2005. QUIJADA, J.; FRYGANAS, C.; ROPIAK, H.M.; RAMSAY, A.; MUELLER-HARVEY, I.; HOSTE, H. Anthelmintic activities against Haemonchus contortus or Trichostrongylus colubriformis from small ruminants are influenced by structural features of condensed tannins. Journal of Agricultural and Food Chemistry, v.63, p.6346-6354, 2015. RAM?REZ-RESTREPO, C. A.; BARRY, T.N. Alternative temperate 71 forages containing secondary compounds for improving sustainable productivity in grazing ruminants. Animal Feed Science & Technology, v. 120, p.179-201, 2005. REED, J.D. Nutritional toxicology of tannins and related polyphenols in forage legumes. Journal of Animal Science, v.73, p.1516-1528, 1995. 72 RUSSELL, J.B.; O'CONNOR, J.D.; FOX, D.G. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal Animal Science, v.70, p.3551-3561, 1992. SANTOS, S.C.; COSTA, W.F.; BATISTA, F.; SANTOS, L.R.; FERRI, P.H.; FERREIRA, H.D. Seasonal variation in the contento os tannins in barks os barbatim?o species. Revista Brasileira de Farmacognosia, v.16, p.552-556, 2006. SCALBERT, A. Antimicrobial properties of tannins. Phytochemistry v.30, p.3875-3883, 1991. SCHOFIELD, P.; MBUGUA, D.M.; PELL, A.N. Analysis of condensed tannins: a review. Animal Feed Science and Technology, v.91, p.21-40, 2001. SILANIKOVE, N.; PEREVOLOTSKY, A.; PROVEZA, F.D. Use of tannin-binding chemicals to assay for tannins and their negative postingestive effects in ruminants. Animal Feed Science and Technology, v.91, p.69-81, 2001. SINGH, B.; BHAT, T.K.; SINGH, B. Potential therapeutic applications of some antinutritional plant secondary metabolites. Journal of Agricultural and Food Chemistry, v.51, p.5579-5597, 2003. STRAIN, S.A.J.; STEAR, M.J. The influence of protein supplementation on the immune response to Haemonchus contortus. Parasite Immunology, v. 23, p. 527-531, 2001. STRADIOTTI JUNIOR, D.; QUEIROZ, A.C.; LANA, R.P. A??o do extrato de pr?polis sobre a fermenta??o in vitro de diferentes alimentos pela tcnica de produ??o de gases. Revista Brasileira de Zootecnia, v. 33, p. 1093-1099, 2004. ST?RM, C.D.; TIEMANN, T.T.; LASCANO, C.E.; KREUZER, M.; HESS, H.D. Nutrient composition and in vitro ruminal fermentation of tropical legume mixtures with costrasting tannin contents. Animal Feed Science and Technology, v.138, p.29-46, 2007. SYKES, A.R. Parasitism and production in farm animals. Animal Production, v.59, p.155- 72, 1994. TERRILL, T.H.; ROWAN, A.M.; DOUGLAS, G.B.; BARRY, T.N. Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture, v.58, p.321?329, 1992. TERRILL, T.H.; WAGHORN, G.C.; WOOLLEY, D.J.; MCNABB, W.C.; BARRY, T.N. Assay and digestion of14C labelled condensed tannins in the gastrointestinal tract of sheep. British Journal of Nutrition, v.72, p.467?477, 1994. TIEMANN, T.T.; LASCANO, C. E.; WETTSTEIN, H.R.; MAYER, A. C.; KREUZER, M.; HESS, H.D. Effect of the tropical tannin-rich shrub legumes Calliandra calothyrsus and Flemingia macrophylla on methane emission and nitrogen and energy balance in growing lambs. Animal, v.2, p.790?799, 2008. 73 TORAL, P. G.; HERV?S, G.; BICHI, E.; BELENGUER, ?., FRUTOS, P. Tannins as fed additives to mod |
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