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Previous issue date: 2015-02-24 / CNPQ / Ageratum conyzoides L. (Asteraceae), conhecida vulgarmente como mentrasto ou catinga de bode e utilizado na medicina popular, como purgante, antitermico, anti-inflamatorio, analgesico, anestesico, antiulcerogenico, antidiarreico, hemorragico e contra disturbios mentais. Poucos estudos acerca do controle de qualidade da materia prima vegetal e da atividade antifungica do oleo essencial foram relatados na literatura. Dessa forma, objetivou-se a realizacao de uma padronizacao farmacognostica, nos ambitos farmacobotanico, quimico e fisico-quimico alem da avaliacao da atividade antifungica. Realizaram-se cortes transversais para analise microscopica da folha, peciolo, caule e raiz; nas folhas foram realizados ainda cortes paradermicos. Seguindo a Farmacopeia Brasileira realizaram-se ensaios fisico-quimicos. Atraves de Cromatografia em Camada Delgada (CCD) foram identificados os grupos de metabolitos. Foi realizada extracao por hidrodestilacao e caracterizacao por Cromatografia Gasosa acoplada a Espectrometria de Massas do oleo essencial das folhas calculando-se o seu rendimento. Avaliou-se a atividade antifungica do oleo essencial frente a especies de Candida. A raiz apresenta epiderme uniseriada com celulas irregulares no parenquima cortical, raios xilematicos bem desenvolvidos e parenquima medular com formas celulares variadas. O caule tem a epiderme unisseriada, com tricomas tectores pluricelulares e sistema vascular colateral. A folha e anfiestomatica com estomatos anisociticos em sua maioria. O mesofilo e assimetrico com uma camada de parenquima palicadico. A nervura central tem contorno biconvexo. A triagem fitoquimica revelou mono e sesquiterpenos, triterpenos e esteroides, flavanoides, cumarinas e alcaloides. O teor de umidade 12,1%, os teores de cinzas totais e insoluveis em acido foram de 9,8 e 1,07% respectivamente. A granulometria permitiu classificar o po como moderadamente grosso. O rendimento do oleo essencial foi de 0,16% (p/v). Os constituintes majoritarios foram Precoceno I e ƒÀ-cariofileno. O oleo de A. conyzoides demonstrou atividade antifungica para diferentes especies de Candidas dentre elas C. glabrata, C. krusei, C. tropicalis, C. guilliermondii, C. parapsilisis, Candida famata e C. albicans. Dessa forma, com a caracterizacao farmacognostica e atividade antifungica para Candida confirmada, A. conyzoides pode ser considerado um material vegetal em potencial para a elaboracao de novos antifungicos. / Ageratum conyzoides L. (Asteraceae), commonly known as ―mentrasto‖ or ―catinga de bode‖ is used in traditional medicine, as a laxative, antipyretic, anti-inflammatory, analgesic, anesthetic, anti-ulcerogenic, anti-diarrheal, antihemorrhagic and against mental disorders. In the literature are few scientific studies concerning the quality control of the raw material from the plant and the antifungal activity of its oil. Therefore, a Pharmacognostic standardization covering the areas of pharmacobotanic, chemistry and physic-chemistry was performed, as well as an evaluation of the antifungal activity. For a microscopic analysis, the leaf, petiole, stem and root were transversely sectioned; further, paradermal sections were undertaken in the leaves. Complying with the Brazilian Pharmacopeia, physic-chemical trails were performed. The identification of metabolites groups was performed through Thin Layer Chromatography (TLC). The essential oil from the leaves was extracted by hydrodistillation and characterized by Gas Chromatography coupled to Mass Spectrometry, and then its yield was calculated. The antifungal activity of the essential oil was evaluated in resistance to some Candida species. The root presents uniseriate epidermis with irregular cells in the cortical parenchyma, well developed xylem and medullary parenchyma with diverse cellular forms. The stem exhibits uniseriate epidermis, with multicellular tector trichomes and collateral vascular system. The leaf is anfiestomatic with anisocytic stomats mostly. The mesophyll is asymmetric with a layer of palisade parenchyma. The central rib has biconvex contour. The phytochemical screening revealed mono and sesquiterpenes, triterpenes and steroids, flavonoids, cumarins and alkaloids. The moisture content of 12.1%, and the levels of total ash and acid-insoluble were 9.8 and 1.07%, respectively. The granulometry classified the powder as moderately thick and the yield of essential oil was 0.16% (w/v). The major constituents were preconeno I and β-caryophyllene. The A. conyzoides oil displayed antifungal activity to several species of Candida, among them C. glabrata, C. krusei, C. tropicalis, C. guilliermondii, C. parapsilisis, C. famata and C. albicans. Hence, with the pharmacognostic characterization and antifungal activity for Candida confirmed, A. conyzoides can be considered a potential plant material for the development of new antifungal agents.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufpe.br:123456789/15381 |
| Date | 24 February 2015 |
| Creators | SANTOS, Rafaela Ferreira dos |
| Contributors | RANDAU, Karina Perrelli |
| Publisher | UNIVERSIDADE FEDERAL DE PERNAMBUCO, Programa de Pos Graduacao em Ciencias Farmaceuticas, UFPE, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Breton |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFPE, instname:Universidade Federal de Pernambuco, instacron:UFPE |
| Rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil, http://creativecommons.org/licenses/by-nc-nd/3.0/br/, info:eu-repo/semantics/openAccess |
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