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Previous issue date: 2015-12-11 / Introdu??o: S?ndrome dos Ov?rios Polic?sticos (SOP), segundo o crit?rio de Rotterdam, est? presente em 6-12% das mulheres em idade reprodutiva, ? caracterizada pelo hiperandrogenismo, resist?ncia ? insulina (RI) e por seu estado inflamat?rio, fatores comumente exacerbados pela presen?a da obesidade e associados com o aumento da genotoxicidade. Alimenta??o saud?vel com implica??o na perda de peso atua restabelecendo as fun??es reprodutivas e metab?licas na SOP, entretanto sua influ?ncia na redu??o da genotoxicidade ? desconhecida. Objetivos: investigar se existem diferen?as entre os marcadores de genotoxicidade e de risco cardiometab?licos em mulheres com SOP e controle, e avaliar a efetividade da interven??o diet?tica nos marcadores de genotoxicidade e de risco cardiometab?licos em mulheres com SOP sobrepeso ou obesas. Metodologia: as participantes tinham faixa et?ria entre 18 e 35 anos. No primeiro estudo transversal foram inclu?das 27 mulheres com diagn?stico de SOP e 20 controles que tiveram seus marcadores antropom?tricos, hormonais, bioqu?micos e inflamat?rios avaliados, al?m da genotoxicidade. O segundo estudo foi um ensaio cl?nico de interven??o diet?tica, com dieta de restri??o cal?rica de 500 Kcal/dia por 12 semanas com 22 mulheres com SOP sobrepeso ou obesas. Dados antropom?tricos, de consumo alimentar, hormonais e bioqu?micos foram avaliados e a genotoxicidade, de ambos os estudos, foi verificada pelo teste do cometa. Resultados: n?o houve diferen?as significativas entre o marcador de genotoxicidade tail intensity (p= 0,18), tail moment (p= 0,76) e tail length ( p=0,109) na SOP quando comparado ao grupo controle. Mulheres com SOP apresentam um pior perfil de risco cardiometab?lico. A interven??o diet?tica reduziu os marcadores genotoxicidade: tail intensity (24,35 ? 5,86 ? pr?-dieta vs. 17,15 ? 5,04 -pos-dieta) e tail moment (20,47 ? 7,85 ? pr?-dieta vs. 14,13 ? 6,29 -p?s-dieta) com p =0,001 para ambos os marcadores. Sem diferen?as no tail length (p=0,07). Provocou tamb?m a perda de peso (3,5%) e redu??o dos marcadores de risco cardiometab?licos, como a RI e o hiperandrogenismo. Conclus?o: mulheres com SOP apresentam pior perfil de risco cardiometab?lico comparado com mulheres controles, entretanto genotoxicidade semelhante. A interven??o diet?tica reduz a genotoxicidade de mulheres com SOP sobrepeso ou obesas, assim como reduz os fatores de risco cardiometab?licos. / Introduction: Polycystic Ovary Syndrome (PCOS), present in 6-12% of women of reproductive age, the criterion of Rotterdam, is characterized by hyperandrogenism, insulin resistance (IR) and its inflammatory state, exacerbated by obesity and factors associated with the increase in damage DNA. Weight loss, combined with healthy eating, acts restoring the reproductive and metabolic functions in the SOP, though its influence in reducing DNA damage in PCOS are unknown. Aim: To investigate whether there are differences between DNA damage markers and factors of cardiometabolic risk in women with PCOS and control, and evaluate the effectiveness of nutritional intervention in DNA damage markers and cardiometabolic risk markers in overweight and obese women with PCOS. Methods: the study was conducted in two studies and the participants were aged between 18 and 35 years. In the first study, a prospective case-control, were included 27 women diagnosed with PCOS and 20 controls. In the second study, clinical trial of nutritional intervention with 12-week calorie-restricted diet 500Kcal / day. The genotoxicity, DNA damage (intensity tail, tail moment and tail length) was evaluated by the comet assay. Anthropometric data, dietary intake, hormonal, biochemical and inflammatory were evaluated in different studies. Results: there was no significant difference between the DNA damage marker tail intensity (p = 0.18), tail moment (p = 0.76) and tail length (p = 0.109) in PCOS when compared to the control group. Data after nutritional intervention in PCOS women with overweight and obesity showed a decrease in DNA damage markers: tail intensity (24.35 ? 5.86 - pre-diet vs. 17.15 ? 5.04 -Post-diet) and tail moment (20.47 ? 7.85 - pre-diet vs. 14.13 ? 6.29 -post-diet) (p <0.001). Reduction of weight (3.5%) and decreased cardiometabolic markers IR and hyperandrogenism. Conclusion: women with PCOS have a worse cardiometabolic risk profile compared to control however similar genotoxicity identified by DNA damage. Nutritional intervention reduced the genotoxicity of overweight and obese women with PCOS, and reduce the factors of cardiometabolic risk.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20762 |
| Date | 11 December 2015 |
| Creators | Soares, Nayara Pereira |
| Contributors | 70155780425, http://lattes.cnpq.br/4311626091295357, Rezende, Adriana Augusto de, 05410395808, http://lattes.cnpq.br/4245215108740331, Arag?o, Cicero Fl?vio Soares, 62001728468, http://lattes.cnpq.br/9657118649043311, Costa, Eduardo Caldas, 05304117417, http://lattes.cnpq.br/1216441676725839, Azevedo, Eduardo Pereira de, 03207095402, http://lattes.cnpq.br/6546119058095940, Machado, Soraya Pinheiro, 56005504304, Lemos, Telma Maria Ara?jo Moura |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM DESENVOLVIMENTO E INOVA??O TECNOL?GICA EM MEDICAMENTOS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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