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Previous issue date: 2015-02-27 / Epilepsy comprehends a category of syndromes characterized by spontaneous and recurrent crises (SRCs), which are a result of intense electric activity, synchronized and rhythmic of neuronal populations of the central nervous system (CNS), and of excessive excitability in the absence of a toxic-metabolic condition or fever 1 2, 3. Experimental studies have shown that SE induction in rodents provokes pronounced inflammatory cerebral reaction involving endothelial cells, glia, and neurons4, 5. Our group has shown that intravenous bone marrow cells administration following lithium-pilocarpine SE induction reduces SRCs, improves cognitive function, and modulates the production of pro-inflammatory cytokines6, 7. The spleen is important to induce cerebral inflammatory response and also is an important target for stem cell intervention therapies8. Splenectomy before a cerebrovascular accident (CVA) significantly reduces the infarction size in the brain9. Moreover, it has been demonstrated that in the pilocarpine model splenectomy decreases the severity of crises, the time to SE onset, and the mortality rate caused by SE10. The aim of the present work was investigating the importance of the interaction between bone marrow mononuclear cells (BMC) and the spleen in epileptic rats. As a result, the animals treated with BMC presented a better capability of memory retrieval in the Morris aquatic maze (MAM) when compared to the treated animals without the spleen. Besides, the treatment did not present the potential of reducing the levels of IL-1 ? or of increasing the levels of IL-10 in the serum and hippocampus of treated rats without the spleen. In conclusion, the work suggests that the spleen could be involved in the therapeutic effect promoted by BMC transplant. / A epilepsia compreende uma categoria de s?ndromes que se caracterizam por crises espont?neas e recorrentes (CERs), que s?o resultados de disparos el?tricos intensos, sincronizados e r?tmicos de popula??es neuronais no sistema nervoso central (SNC) e de excitabilidade excessiva, na aus?ncia de condi??o t?xico-metab?lica ou febril.1 2, 3 Pesquisas experimentais mostram que a indu??o do Status Epil?pticos (SE) em roedores provoca rea??o inflamat?ria cerebral pronunciada envolvendo as c?lulas endoteliais, glia e neur?nios.4, 5 Nosso grupo mostrou que a administra??o de c?lulas da medula ?ssea por via intravenosa depois da indu??o do SE por l?tio-pilocarpina ? capaz de reduzir as CERs, melhorar a fun??o cognitiva e modular a produ??o de citocinas pr?-inflamat?rias.6, 7 O ba?o ? importante na indu??o da resposta inflamat?ria cerebral e tamb?m ? um alvo importante para a interven??o de c?lulas-tronco.8 A esplenectomia antes do acidente vascular cerebral (AVC) reduz significativamente o tamanho do infarto no c?rebro.9 Foi tamb?m demonstrado que no modelo da pilocarpina, a esplenectomia diminuiu a gravidade das crises, o tempo para o in?cio do SE, e a taxa de mortalidade causada pelo SE.10 O objetivo do presente trabalho ? investigar a import?ncia da intera??o entre c?lulas mononucleares da medula ?ssea (CMMO) e o ba?o em ratos epil?pticos. Como resultado do trabalho nossos animais tratados com as CMMO apresentaram melhor capacidade de reter a mem?ria no teste do LAM quando comparados aos animais tratados sem o ba?o. Al?m disso, nesses animais o tratamento n?o teve o potencial de reduzir as taxas de IL-1 ? no soro e nem de aumentar os n?veis de IL-10 no soro e no hipocampo de ratos tratados e sem o ba?o. Concluindo, o trabalho sugere que o ba?o possa estar envolvido modulando a cascata inflamat?ria no efeito terap?utico promovido pelo transplante de CMMO.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6256 |
| Date | 27 February 2015 |
| Creators | Silva, Daniele Vieira da |
| Contributors | Costa, Jaderson Costa da |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina/Pediatria e Sa?de da Crian?a, PUCRS, Brasil, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 3098206005268432148, 600, 600, 600, -8624664729441623247, -7027121703779065955 |
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