Study aim is to study endogenic and exogenic factors preconditioning morphogenesis of linseed flax in vitro. Linseed flax studies in vitro were carried out at the Laboratory of Genetic – Biotechnological of Lithuanian University of Agriculture in 2004-2006. Factors which influence morphogenetic capability of linseed flax cultivars ‘Lirina’, ‘Barbara’ and ‘Szaphir’ in tissue culture were investigated. Two different types of explants – stem segments and hypocotyls – of the three genotypes were cultivated in nutrient media differing in their macro and micro salts concentration, vitamins and different levels of growth regulators. Our results showed that intensity of morphogenesis was affected not only by exogenous growth regulators, but also by the type of explant and genotype. Hypocotyls-derived callus gave the better results than that stem-segment-derived callus on all tested media. Four-weeks-old callus showed the maximum shoot regeneration frequency. The best morphogenetic capabilities were demonstrated for cultivar ‘Szaphir’. The largest number of shoots was produced by all the cultivars on MSB5 nutrient medium supplemented with cytokinin 2iP (2.0 mg 1-1). The master’s final work comprises 50 pages, including 3 tables, 10 figures and 66 literature references.
| Identifer | oai:union.ndltd.org:LABT_ETD/oai:elaba.lt:LT-eLABa-0001:E.02~2006~D_20060608_111352-26647 |
| Date | 08 June 2006 |
| Creators | Katauskytė, Lina |
| Contributors | Bobinas, Česlovas, Lazauskas, Petras, Sliesaravičius, Algirdas, Pranskietienė, Irena, Venskutonienė, Egidija, Motuzas, Algirdas, Burbulis, Natalija, Lithuanian University of Agriculture |
| Publisher | Lithuanian Academic Libraries Network (LABT), Lithuanian University of Agriculture |
| Source Sets | Lithuanian ETD submission system |
| Language | Lithuanian |
| Detected Language | English |
| Type | Master thesis |
| Format | application/pdf |
| Source | http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20060608_111352-26647 |
| Rights | Unrestricted |
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