Study aim is to study endogenic and exogenic factors preconditioning morphogenesis of linseed flax in vitro. Linseed flax studies in vitro were carried out at the Laboratory of Genetic – Biotechnological of Lithuanian University of Agriculture in 2004-2006. Factors which influence morphogenetic capability of linseed flax cultivars ‘Lirina’, ‘Barbara’ and ‘Szaphir’ in tissue culture were investigated. Two different types of explants – stem segments and hypocotyls – of the three genotypes were cultivated in nutrient media differing in their macro and micro salts concentration, vitamins and different levels of growth regulators. Our results showed that intensity of morphogenesis was affected not only by exogenous growth regulators, but also by the type of explant and genotype. Hypocotyls-derived callus gave the better results than that stem-segment-derived callus on all tested media. Four-weeks-old callus showed the maximum shoot regeneration frequency. The best morphogenetic capabilities were demonstrated for cultivar ‘Szaphir’. The largest number of shoots was produced by all the cultivars on MSB5 nutrient medium supplemented with cytokinin 2iP (2.0 mg 1-1). The master’s final work comprises 50 pages, including 3 tables, 10 figures and 66 literature references.

Tyrimai atlikti 2005-2007m. LŽŪU Genetikos biotechnologijos laboratorijoje. Tirti veiksniai, įtakojantys vasarinių rapsų (Brassica napus var. oleifera) dvigubų haploidų linijų NL-302-01, NL-302-02, NL-302-25 somatinės embriogenezės procesą nesubrendusių zigotinių gemalų kultūroje. Tirtų genotipų eksplantai (zigotniai gemalai) kultivuoti maitinamosiose terpėse, besiskiriančiose terpės rūgštingumu. Eksplantai iš donorinių augalų tirti 14-29 amžiaus dienų po apdulkinimo. Nustatyta, kad vasarinių rapsų somatinės embriogenezės potencialas priklauso ne tik nuo nesubrendusio zigotinio gemalo amžiaus po apdulkinimo, bet ir nuo eksplanto genotipo ir terpės rūgštingumo. Daugeliu atveju didžiausią įtaką indukcijos intensyvumui turėjo genotipas. Didžiausiomis pirminės somatinės embriogenezės galimybėmis pasižymėjo linijos NL-302-25 gemalai 20-21 dienos amžiaus. Antrinės somatinės embriogenezės metu nustatyta, kad indukcijos potencialas buvo didesnis nei pirminės somatinės embriogenezės metu. Ekspalnto amžius įtakos soamtinių embrioidų formavimo kiekiu neturėjo. / The research was carried out in 2005-2007 in the laboratory of genetics and biotechnology of Lithuanian university of Agriculture Factors influencing double haploid lines’ NL-302-01, NL-302-02, NL-302-25 somatic embryogenesis in the culture of premature zygotic germs of summer rape (Brassica napus var. oleifera) were studied. The explants of the studied genotypes (premature germs) were cultivated in the nutritional environments differencing in pH. The explants from the donor plants were taken for the period of 14-29 days. It was determined that the potential of the somatic embryogenesis of the summer rape depends on not only on the age of the premature zygotic germ after the pollination, but also on the genotype and medium pH of the explants. In most cases the biggest effect on the intensity of the induction was caused by genotype. Line NL-302-25 cultivated 20-21 days after the pollination had the biggest potential of primary somatic embryogenesis. During the secondary somatic embryogenesis it was determined that the potential of induction was greater than during the primary somatic embryogenesis. The age of the explant had no effect on the quantity of formation of somatic embryos.