Não consta resumo na publicação. / The gene encoding actin in the cellulolytic filamentus fungus Trichoderma reesei has been isolated and sequenced. The nucleotide sequence reveals that the gene is composed of 6 exons separated by 5 introns within the coding region. The positions of the introns were predicted by comparison of sequence homology to the genes coding for actin with known amino acid sequence and by identification of splice-site signal sequences. The actin protein of Trichoderma reesei shows extensive homology to the actins of other fungi E. nidulans, 95% , T. lanuginosus, 92% and S. pombae. The T. reesei actin promoter has a CT-rich region, CAAT and GC. There is no obvious TATA sequence in the T. reesei actin promoter. The absence of TATA-like sequence were also observed in anothers genes of T. reesei. An important aspect in molecular biology of filamentous fungi is the analysis, under a specific metabolic events, of the mechanism(s) regulating the expression of constitutive and induced genes. The filamentous fungus Trichoderma reesei is considered to be one of the most efficient producer of cellulase, and it serves as a model system for enzymatic cellulose hydrolysis. Expression of the cellulase genes are stringently regulated by the carbon source. Growth on cellulose results in induction of the cellulase transcripts, whereas glucose strongly represses their expression. The availability of a constitutive expressed genes of T. reesei provides not only important information regarding the molecular biology of the fungi, but also is essential for a better understanding of the mechanism(s) controlling the expression of the cellulase transcripts. Under inductive process of the of the major cellulase transcript (cbh1) and its repression by glucose, actin mRNA is constitutively expressed. The present results should be useful for further structural and functional analysis of the elements involved in inductive and constitutive expression of cellulase and actin transcripts.
| Identifer | oai:union.ndltd.org:usp.br/oai:teses.usp.br:tde-18092015-165016 |
| Date | 27 October 1993 |
| Creators | Matheucci Junior, Euclides |
| Contributors | Maia, José Carlos da Costa |
| Publisher | Biblioteca Digitais de Teses e Dissertações da USP |
| Source Sets | Universidade de São Paulo |
| Language | Portuguese |
| Detected Language | English |
| Type | Dissertação de Mestrado |
| Format | application/pdf |
| Rights | Liberar o conteúdo para acesso público. |
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