Orientador: Eduardo Dias de Andrade / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-07-14T04:11:00Z (GMT). No. of bitstreams: 1 Souza_PatriciaMedeirosde_M.pdf: 1293424 bytes, checksum: d64ef9d754021c735cf05b8091359f8d (MD5) Previous issue date: 1992 / Resumo: Este trabalho teve por objetivo avaliar a influência da betametasona, um corticosteroide bastante empregado como antiinflamatorio em Odontologia, sobre os valores de glicemia em jejum, em ratos normais e diabéticos-aloxãnicos. Para tal, foram utilizados 40 ratos (Rattus novergicus albino, raça Wistar heterogenética), machos, adultos (90 dias) e com um peso em média de 300 g, que foram divididos em 2 grupos (normais e diabéticos) e estes em 2 sub-grupos (tratados com solução salina ou betametasona, em dose única). A glicemia foi medida nos tempos 0 (zero) e 30, 60, 120 e 240 minutos após o tratamento de auto-monitoramento de glicose sanguínea. Os resultados mostraram não ocorrer uma diferença estatisticamente significante (p < 0,05) nos níveis glicêmicos de ratos normais ou diabéticos, quando tratados com solução salina ou betametasona, estudo, na dose de 0,1. mg/kg, nos diferentes tempos de estudo / Abstract: The aim of this paper was study the influence of betamethasone, a corticosteroid very used like anti-inflammatory in Dentistry, over the values of fasted glycemia, in normal and alloxan-diabetics rats. For this purpose, there were been used 40 rats (Rattus novergicus albino, race Wistar heterogenetic), male, grow-up (90days) and with the weight of 300 g in average, wich were divided in 2 groups (normal and diabetic) and these ones in 2 subgroups (the subgroup that received the treatment of saline or the other, betamethasone, in single dosage). The glycemia was measured using the method of self glucose monitoring. The beginning time was 0 (zero) and the others were 30, 60, 120 and 240 minutes after treatment. The results showed that did'nt appear a significant statistic difference (p < 0,05) in the glycemic level of normal and diabetics rats, when they received saline or betamethasone in a single dosage of 0,1 mg/kg, in differents times of study / Mestrado / Farmacologia / Mestre em Odontologia

HLA genotypes were determined and prospective investigations performed on members of 166 families, each including a Type 1 diabetic proband. A computer-based system (Genobase) was created for data storage and analysis. Newly-diagnosed cases ascertained in East Berkshire (incidence rate: 17.8 x 10^-5/year) and additional data from two other studies contribute to some analyses. Major findings include non-random assortment of HLA-B8 within families and a tendency for HLA-identical siblings of diabetic probands to be younger than expected. Discrete multivariate analysis and other methods show no major genetic heterogeneity within Type 1 diabetes. However, there is minor heterogeneity affecting the interaction of age and seasonal variation in incidence of the diabetes. Furthermore, the HLA genotype B8,15 is here shown to be significantly more frequent in Type 1 diabetic probands than expected assuming Hardy-Weinberg equilibrium. With support from other internal and published data, it is concluded that susceptibility to Type 1 diabetes is genetically multifactorial. Increased prevalence of organ-specific autoimmunity is confirmed in the families, and differences in distribution of islet-cell antibodies (ICA) and thyroid/gastric antibodies demonstrated. Five subjects, ICA-positive from the first test, developed diabetes after up to 30 months, demonstrating prolonged latency. Quantitative risk of diabetes is estimated (separately) in terms of ICA results and of HLA genotypes. Non-diabetic siblings of affected probands are investigated for evidence that an HLA-linked immune response gene might contribute to susceptibility. Response patterns to previous polio vaccination give some support, but the excess of thyroid/gastric antibodies in the siblings is not due to such a gene. The dissertation concludes with discussion of aetiological hypotheses and indications for further work.

The purpose of this study was to answer the question, 'Will diabetic patients taught by means of a "Diabetic Teaching Tool" demonstrate a higher level of learning about self-care, than patients taught in the institution's usual manner?' The answer to the question was sought by comparing the self-care knowledge and skills of two groups of diabetic patients admitted to a suburban general hospital which, prior to the study, offered no planned programme of diabetic patient education. All diabetic patients admitted to this hospital over a six month period were screened for eligibility to participate in the study according to criteria stated by the researcher. Eligible patients admitted in the first three months were designated as control subjects; those in the last three months as experimental subjects. The twenty subjects in the control group were taught in an unplanned manner, based upon whether and/or what instructions were deemed pertinent by their nurses. A "Diabetic Teaching Tool"-- designed by the researcher and administered by each patient's own nurse(s)-- was used to instruct the twenty experimental subjects. After discharge, each of the forty subjects was visited by the researcher, at which time a profile sheet was completed and a test of diabetic learning administered. Demographic and diabetic characteristics of the subjects-obtained from the patient profile sheets-- were analyzed and described in terms of distributions, medians and/or means. The test results were subjected to t-test analyses on several dimensions. And a number of demographic and diabetic traits were compared with their respective test scores by means of the Pearson Product Moment Correlation Coefficient. The data supported the following conclusions: 1. Diabetic patients taught by means of the "Diabetic Teaching Tool" demonstrated a significantly higher level of learning about self-care than did patients taught in the unplanned manner. 2. Statistically significant differences were found between test scores of patients taught with the "Diabetic Teaching Tool" and those receiving unplanned instruction regardless of the duration of their diabetes. Thus 'old' diabetics were able to derive as much benefit from the teaching tool as were 'new' diabetics. 3. The level of learning demonstrated by patients taught with the "Diabetic Teaching Tool" appeared to be independent of the following factors: age at time of teaching and testing, previous education, and age at onset of diabetes; each of these factors was significantly related to the level of learning of patients receiving unplanned instruction. 4. Diabetic patients taught by means of the "Diabetic Teaching Tool" cited the nurse as a valuable source of information regarding diabetic management more than five times as frequently as did patients receiving unplanned instruction. Based upon these findings, several implications for nursing practice and recommendations for further research were suggested. / Applied Science, Faculty of / Nursing, School of / Graduate

Orientador: Oslei Paes de Almeida / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-07-17T13:53:57Z (GMT). No. of bitstreams: 1 Jorge_AntonioOlavoCardoso_M.pdf: 2660517 bytes, checksum: 72d9dad993dae7a9536667f85465a9ef (MD5) Previous issue date: 1988 / Resumo: Os resultados deste trabalho confirmaram que a permeabilidade vascular na pele e músculo cremaster está diminuída nos ratos diabéticos. Por outro lado, nos mesmos animais, não se observou alterações da permeabilidade vascular dos vasos gengivais envolvidos na formação do fluido gengival. Estes resultados indicam que a insulina não participa efetivamente na permeabilidade dos vasos gengivais, como ocorre em outros tecidos / Abstract: Diabetic rats showed a decreased vascular permeability of the skin and cremaster muscle vessels. On the other hand, the same animaIs did not present similar alterations in the vessels involved in the formation of gingival fluid. These results suggest that insulin does not play an important role in the permeability of the gengival vessels / Mestrado / Patologia / Mestre em Ciências

Orientador : Paulo Afonso Ribeiro Jorge / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas / Made available in DSpace on 2018-07-18T19:20:45Z (GMT). No. of bitstreams: 1 Chaves_FatimaRegina_D.pdf: 4172158 bytes, checksum: 038835f91daa6259ed7d6a6668bd0484 (MD5) Previous issue date: 1993 / Resumo: Neste estudo, analisamos os corações de cento e trinta e sete indivíduos diabéticos necropsiados, quanto às suas manifestações clínicas e alterações anátomo-patológicas. Entendemos que a hiperglicemia produz efeitos deletérios diretos, dirigidos às enzimas da miocélula que regulam o equilíbrio iônico e contrátil, resultando em instabilidade elétrica e depressão do miocárdio. A longo prazo, os efeitos da hiperglicemia no coração são decorrentes de alterações da permeabilidade dos pequenos vasos intramiocárdicos que, através de múltiplas alterações dos principais mecanismos homeostáticos, lesam progressivamente o interstício e a miocélula. As manifestações clínicas mais freqüentes são: a precordialgia típica, na ausência de coronariopatia obstrutiva que a justifique, e os sintomas decorrentes da insuficiência ventricular, inicialmente diastólica. Exames subsidiários de rotina como, o eletrocardiograma de repouso e o exame radiológico convencional, não constituem recursos diagnósticos adequados e suficientes, devido à pequena extensão e segmentaridade das lesões. O exame anátomo-patológico identifica as lesões prevalentes e peculiares, mas não constituem lesões patognomônicas. Entretanto, uma característica exclusiva do diabetes é a natureza focal das lesões observadas. Esta segmentaridade permite que a Cardiomiopatia Diabética possa ser diagnosticada ao exame anátomo-patológico, mesmo quando associada à cardiopatias de outra etiologia, cuja freqüência é muito elevada no diabetes. Observamos que, em 137 indivíduos estudados, as lesões focais corresponderam a 61.31% e os indivíduos portadores de tais lesões seguramente eram possuidores de Cardiomiopatia Diabética, que acreditamos ser decorrente da lesão dos pequenos vasos intra miocárdicos. Entre estes, 16.78% são diabéticos sem outras patologias cardiovasculares e 71.53% são diabéticos que possuem outros mecanismos de lesão, principalmente representados pela Hipertensão Arterial Sistêmica e Coronariopatia Obstrutiva. Em todas as lesões anátomo-patológicas observadas nos indivíduos exclusivamente diabéticos, houve o predomínio do mau controle glicêmico. O meio metabólico hiperglicêmico também foi fundamental para a ocorrência da macroangiopatia ateroesclerótica nas formas obstrutivas graves, superiores a 50%, principalmente em obstruções entre 70 e 100%. / Abstract: In this study, we analysed the hearts from 137 diabetic individuals necropsied as to their clinical manifestations and anatomo-pathological alterations. We believe that hyperglycemia causes direct deleterions effects towards the myocyte enzimes, which regulate the ionic and contractile balance, leading to an electric instabilyty and myo cardial depression. At long term the effects of hyperglycemia on heart are due to alterations of the permeability of intramyocardial small vessels, which through multiple alterations of main homeostatic mechanism progressively injure interstice and myocyte. The most frequent clinical manifestations are: typical pre cordialgy in the absence of obstructive coronaryopathy which justifies it and the symptoms and signals resulting from ventricular insufficiency, initialy diastolic. Routine subsidiary exams, such as electrocardiogram at rest and usual radiologic exam, do not constitute neither appropriate not sufficient aid for diagnosis since the lesions are small and focal. The anatomo-pathological exam identifies the prevalent lesions but not the pathognomonic ones of diabetes. However, the focal nature of the observed lesions establishes an exclusive characteristic of diabetes. Due to such nature, Diabetic Cardiomypathy can be diagnosed by anatomo-pathological exam, even when it is associated to other types of cardiopathy, which frequency is very high in diabetes. We observed that in studied, focal the 137 individuals studied, focal lesions corresponded to 61.31 % of the total cases. Such individuals suggered from Diabetic Cardiomiopathy, which we believe to be resulting from the lesion of intramyocardic small vessels. From there groups, 16.78 % are diabetics without any other associated cardiovascular pathologies and 71.53 % are diabetics with other mechanisms of lesion, mainly represented by Systemic Arterial Hypertension and Obstructive Coronariopathy. A poor glicemic control was predominant in the anatomo-pathological lesions observed in exclusively diabetic individuals. The hyperglycemic metabolic medium was also essential for the of arteriosclerotic severe macroangiopathy in occurrence obstructive forms, greater than 50.00 % and mainly between 70 and 100 %. / Doutorado / Doutor em Medicina

Thesis Title Exploring new methodologies to identify disease-associated variants in African populations through the integration of patient genotype data and clinical phenotypes derived from routine health data: A case study for Type 2 Diabetes Mellitus patients in the Western Cape Province, South Africa. Abstract Introduction There is poor knowledge on the genetic drivers of disease in African populations and this is largely driven by the limited data for human genomes from sub-Saharan Africa. While the costs of generating human genomic data have gone down significantly, they are still a barrier to generating large scale African genomic data. This project is therefore a proof-of-concept pilot study that demonstrates the implementation of a cost-effective, scalable genotyped virtual cohort that can address population level genomic questions. Methods We optimised a tiered informed consent process that is suitable for the cohort study design and adapted it to conducting human genomic research in the African context. We used an existing dataset to explore statistical methods for modelling longitudinal routine health data into a standardised phenotype for genome wide association studies (GWAS). We then conducted a feasibility study and piloted the tiered informed consent process, DNA collection by buccal swab and DNA extraction from buccal swabs and peripheral blood samples. DNA samples were genotyped for approximately 2.2 million variants on the Infinium™ H3Africa Consortium Array V2. Genotyping quality control (QC) was done in Plink 1.9 and genome wide imputation on the Sanger Imputation Service. We demonstrated successful variant calling and provide aggregate statistics for known aetiological variants for type 2 diabetes and severe COVID-19 as well as demonstrating the feasibility of running nested case-control GWAS with these data. Results We demonstrate the use of routine health data to provide complex phenotypes to link to genotype data for both non-communicable diseases (diabetes) and infectious diseases (Tuberculosis, HIV and COVID-19). 459 participants consented to providing a DNA sample and access to their routine health data and were included in the feasibility study. A total of 343 DNA samples and 1782023 genotyped variants passed quality control and were available for further analysis. While most of the cohort population clustered with the 1000 genomes African population, principal component analysis showed extensive population admixture. For the COVID-19 analysis, we identified 63 cases of severe COVID-19 and 280 controls, and for the type 2 diabetes analysis we identified 93 cases and 250 controls using the routine health data of participants in the cohort. While the sample sizes were insufficient for a GWAS we were able to evaluate known type 2 diabetes mellitus and COVID-19 variants in the study population. Conclusion We have described how we conceptualised and implemented a genotyped virtual population cohort in a resource constrained environment, and we are confident that this design and implementation are appropriate to scale up the cohort to a size where novel health discoveries can be made through nested case-control studies. In the interim we demonstrate the analysis and validation of aetiological variants identified in other studies and populations.

Com o objetivo de verificar se as proteínas do sistema GH-IGF-IGFBP e o receptor do fator de crescimento insulina símile tipo I (IGF-IR) estão envolvidos na etiopatogenia do Diabetes Melito tipo 1 (DM1) , foram estudados 23 pacientes prépúberes portadores de DM1 em diferentes fases do diagnóstico (Grupo A: tempo de diagnóstico <= 6 meses; Grupo B: tempo de diagnóstico > 6 meses) e 10 indivíduos pré-púberes sadios como grupo controle (Grupo C). A expressão do mRNA do IGFIR realizada através do ensaio molecular de RT-PCR nos linfócitos periféricos T e B não demonstrou diferenças estatisticamente significantes nos linfócitos T quando comparados indivíduos diabéticos e controles, sugerindo que a ativação imunológica destas células seja independente da ação do IGF-IR. Observou-se uma maior expressão do mRNA do IGF-IR dos linfócitos B de pacientes diabéticos em relação ao grupo controle (p < 0,05). A avaliação das proteínas do sistema GH-IGF-IGFBP não demonstrou diferença estatística significante entre os grupos. Estes achados, associados à presença de auto-anticorpos para o DM1 (ICA, anti-GAD e anti-IA2) sugerem fortemente o papel do IGF-IR na ativação dos linfócitos B envolvidos na etiopatogenia do DM1 / Aiming to verify if GH-IGF-IGFBP proteins system and insulin-like growth factor type I receptor (IGF-IR) are implicated on pathofisiology of type 1 Diabetes Mellitus (DM1), we studied 23 prepubertal patients with DM1 on different stages of diagnosis (Group A: time of diagnosis <= 6 months; Group B: time of diagnosis > 6 months) and 10 prepubertal healthy subjects as control group (Group C). The RT-PCR molecular assay for IGF-IR mRNA on peripheral T and B lymphocytes didn\'t show statistical differences between the groups when T cells were analyzed. We found an increase of IGF-IR mRNA expression on B cells from diabetic patients when compared to healthy subjects (p< 0,05). There were no differences in the GH-IGF-IGFBP proteins system levels between the groups. Our study suggest that IGF-IR in association with diabetes-related autoantibodies (ICA, anti-GAD and anti-IA2) presence could activate B cells involved on pathofisiology of DM1

Com o objetivo de verificar se as proteínas do sistema GH-IGF-IGFBP e o receptor do fator de crescimento insulina símile tipo I (IGF-IR) estão envolvidos na etiopatogenia do Diabetes Melito tipo 1 (DM1) , foram estudados 23 pacientes prépúberes portadores de DM1 em diferentes fases do diagnóstico (Grupo A: tempo de diagnóstico <= 6 meses; Grupo B: tempo de diagnóstico > 6 meses) e 10 indivíduos pré-púberes sadios como grupo controle (Grupo C). A expressão do mRNA do IGFIR realizada através do ensaio molecular de RT-PCR nos linfócitos periféricos T e B não demonstrou diferenças estatisticamente significantes nos linfócitos T quando comparados indivíduos diabéticos e controles, sugerindo que a ativação imunológica destas células seja independente da ação do IGF-IR. Observou-se uma maior expressão do mRNA do IGF-IR dos linfócitos B de pacientes diabéticos em relação ao grupo controle (p < 0,05). A avaliação das proteínas do sistema GH-IGF-IGFBP não demonstrou diferença estatística significante entre os grupos. Estes achados, associados à presença de auto-anticorpos para o DM1 (ICA, anti-GAD e anti-IA2) sugerem fortemente o papel do IGF-IR na ativação dos linfócitos B envolvidos na etiopatogenia do DM1 / Aiming to verify if GH-IGF-IGFBP proteins system and insulin-like growth factor type I receptor (IGF-IR) are implicated on pathofisiology of type 1 Diabetes Mellitus (DM1), we studied 23 prepubertal patients with DM1 on different stages of diagnosis (Group A: time of diagnosis <= 6 months; Group B: time of diagnosis > 6 months) and 10 prepubertal healthy subjects as control group (Group C). The RT-PCR molecular assay for IGF-IR mRNA on peripheral T and B lymphocytes didn\'t show statistical differences between the groups when T cells were analyzed. We found an increase of IGF-IR mRNA expression on B cells from diabetic patients when compared to healthy subjects (p< 0,05). There were no differences in the GH-IGF-IGFBP proteins system levels between the groups. Our study suggest that IGF-IR in association with diabetes-related autoantibodies (ICA, anti-GAD and anti-IA2) presence could activate B cells involved on pathofisiology of DM1

OLIVEIRA, P. B. Consumo de adoçantes e produtos dietéticos por indivíduos com diabetes mellitus tipo 2, atendidos pelo Sistema Único de Saúde em Ribeirão Preto SP. 2009. 84 f. Dissertação (Mestrado em Saúde na Comunidade). Faculdade de Medicina de Ribeirão Preto-USP. Devido às suas complicações crônicas, o diabetes mellitus (DM) apresenta impacto considerável como problema de saúde pública pela morbidade, mortalidade e custos no seu tratamento. Para obter um bom controle metabólico, a educação alimentar é um dos pontos fundamentais no seu tratamento. O uso de adoçantes e alimentos dietéticos é importante para as pessoas com DM, apesar de serem dispensáveis na alimentação. Este setor cresceu nos últimos anos e atualmente 35% dos lares brasileiros consomem algum tipo de produto light ou diet. Diante das dificuldades e do pouco conhecimento dos indivíduos com DM sobre o uso destes produtos, verificou-se a necessidade de coletar informações sobre o assunto para subsidiar programas educativos. Este estudo tem como objetivo analisar o consumo de adoçantes e produtos dietéticos por indivíduos com diabetes tipo 2 (DM2), atendidos pelo Sistema Único de Saúde em Ribeirão Preto-SP. Os dados foram obtidos por meio de um questionário envolvendo variáveis sóciodemográficas, clínicas, relacionadas à hábitos alimentares e ao uso de produtos dietéticos e adoçantes, aplicado em uma amostra de 120 pacientes, sendo 60 do sexo feminino e 60 do sexo masculino, estratificados em adultos e idosos. Metade da amostra foi entrevistada no Centro de Saúde Escola da FMRPUSP (nível secundário de atendimento) e a outra metade nos Núcleos de Saúde da Família (nível primário de atendimento), vinculados à FMRP-USP, após concordância em participar do estudo e assinatura do termo de consentimento. As análises foram realizadas com ajuda do software Epi-Info e consistem na descrição das variáveis de interesse, com realização de comparação, entre sexos, faixa etária e nível de atendimento (primário e secundário). Aplicou-se o teste exato de Fisher e fixou-se o valor de p em 0,05. A média de idade dos entrevistados foi de 63,1 anos (± 10,5) e a duração do diagnóstico médio de DM de 9,8 anos (± 8,6). Os resultados encontrados mostram que a maioria apresentou sobrepeso ou obesidade. O uso de adoçante é freqüente na população em estudo, com predomínio do tipo líquido, e o uso de produtos dietéticos é menor, porém significativo, com predomínio dos refrigerantes. O diagnóstico do DM foi determinante para o uso de ambos e o critério de seleção mais utilizado para os usuários de adoçante foi o sabor. É pequena a porcentagem da população estudada que sabe a diferença entre produto diet e light, que tem o hábito de ler o rótulo dos alimentos e que se preocupa com a quantidade utilizada de adoçante. Conclui-se que é uma necessidade a inclusão de informações sobre o uso adequado de adoçantes e produtos dietéticos nas atividades assistenciais aos pacientes com diabetes nos diversos níveis do Sistema Único de Saúde, levando em consideração as características educacionais da população assistida. / OLIVEIRA, P. B. Consumption of sweeteners and dietetic foods by individuals with type 2 diabetes mellitus, assisted by the National Health System (SUS) in Ribeirão Preto - SP. 2009. 84 f Dissertation (Master Degree). Faculdade de Medicina de Ribeirão Preto-USP. Due to its chronic complications, diabetes mellitus presents considerable impact as public health problem for the morbidity, mortality and costs in its treatment. To get a good metabolic control, the nutritional education is one basic point for its treatment. The use of sweeteners and dietetic foods is important for persons with diabetes, although dispensable in the diet. This sector had an important growth in recent years and presently, 35% of the Brazilian homes consume some type of light or diet products. Ahead of the difficulties and the limited knowledge of individuals with diabetes about the use of these products, it was identified the necessity to collect information about this issue to subsidize educative programs. This study has the objective to analyze the consumption of sweeteners and dietetic foods by individuals with type 2 diabetes, assisted by the Brazilian National Health System (SUS Sistema Único de Saúde), in the city of Ribeirão Preto-SP. Data were collected through a questionnaire involving variables as: socio-demographic, life style, history of illness and consumption of dietetic foods and sweeteners in a sample of 120 diabetic patients, being 60 women and 60 men, stratified in adults and elderly. Half of the sample was interviewed in the CSE of the FMRP-USP and the other half in the NSF, tied to the FMRP-USP, after signature of the informed consent form. The analysis was carried through the software Epi-Info and consisted of description of variables of interest, with comparison between sex, age-group and level of attendance. The test of Fisher was applied and was fixed the value of p as 0,05. The mean age of the individuals was 63,1 years (± 10,5) and the average duration of the diagnosis of diabetes was 9,8 years (± 8,6). The sample is characterized by individuals classified between overweight and obesity. The use of sweeteners is very frequent by the population in study, with predominance of the liquid type, and the use of dietetic foods is less common, but significant, with predominance of soft drinks. The diagnosis of diabetes was determinant for starting the use of both and the used criterion for selection of the sweetener is its flavor. The percentage of the population that knows the difference between diet and light products, that has the habit to read labels and that is worried with the amount used of sweetener, is small. In conclusion, there is a need to include informations about the adequate use of sweeteners and dietetic products in the health care activities for patients with diabetes in the different levels of the Brazilian National Heath System, taking in consideration the educational characteristics of the target population.

A associação entre o diabetes e a periodontite produz uma descarga de proteínas inflamatórias que pode ser refletida na saliva. Com base nisso, o objetivo desse estudo foi mensurar as concentrações salivares de interleucina-6 (IL-6), metaloproteinase da matriz-8 (MMP-8) e osteoprotegerina (OPG) em pacientes com periodontite crônica associada ou não ao diabetes mellitus tipo 2. Um total de 90 indivíduos foi dividido em quatro grupos: saudáveis (Controle, n=22), pacientes com doença periodontal (DP, n=24), apenas com diabetes mellitus (DM, n=20) e com doença periodontal e diabetes mellitus (DP+DM, n=24). Dados clínicos e metabólicos foram registrados. Amostras de saliva não-estimulada foram analisadas pelo ensaio imunoenzimático (ELISA). Diferenças estatisticamente significantes foram detectadas entre os grupos para todos marcadores (p<0,05). Em relação à IL-6, diferenças estatisticamente significantes foram encontradas comparando os grupos Controle com DP e com DP+DM, e entre os grupos DP+DM com DM (p<0,005). Para MMP-8, a média das concentrações do grupo Controle foi significativamente menor que em todos os grupos doentes (p<0,01) e nenhuma diferença entre os grupos doentes foi detectada. Para OPG, diferenças estatisticamente significantes foram encontradas entre os grupos Controle com DP e entre Controle com DM (p<0,05). Todos os parâmetros clínicos foram estatisticamente significantes entre os grupos (p<0,001), exceto supuração. No grupo DP, SS (sangramento à sondagem) mostrou uma correlação positiva com as concentrações de IL-6 (r=0,48; p<0,05), PS>=7 (profundidade de sondagem>=7mm) correlacionou-se positivamente com as concentrações de MMP-8 (r=0,46; p<0,05), e os níveis de HbA1c também correlacionaram-se positivamente com as concentrações de IL-6 (r=0,54; p<0,000). Concluindo, a saliva é um adequado substrato para identificação de biomarcadores inflamatórios em pacientes periodontais com ou sem diabetes. A IL-6 é um biomarcador candidato para periodontite e periodontite associada ao diabetes na saliva. Além disso, conclui-se que a super-expressão de MMP-8 e OPG pode acionar o aumento do colapso periodontal observado em indivíduos com diabetes tipo 2. / Background: Association of diabetes and periodontitis produces an inflammatory proteins discharge that can be reflected in saliva. The aim of this study was to measure salivary concentrations of interleukin-6 (IL-6), matrix metalloproteinase-8 (MMP-8) and osteoprotegerin (OPG) in patients with chronic periodontitis associated or not to type 2 diabetes mellitus. Methods: A total of 90 subjects was divided in four groups: healthy (Control, n=22), patients with Periodontal Disease (PD, n=24), Diabetes Mellitus only (DM, n=20), Periodontal Disease and Diabetes Mellitus (PD+DM, n=24). Clinical and metabolic data were recorded. Non-stimulated saliva samples were analyzed by enzyme-linked immunosorbent assay (ELISA). Results: Significant differences were detected between groups for the biomarkers (p<0.05). Regarding IL-6, significant differences were found comparing Control with PD and with PD+DM, and comparing PD+DM with DM groups (p<0.005). For MMP- 8, the concentrations mean of the Control group was significantly lower than all the diseased groups (p<0.01), and no differences between diseased groups were detected. For OPG, significant differences were found between Control and PD, and between Control and DM groups (p<0.05). All clinical parameters were significant between groups (p<0.001), except suppuration. In PD group, BOP (bleeding on probing) showed positive correlation with IL-6 concentrations (r=0.48; p<0.05), PPD>=7 (pocket depth>=7mm) correlated positively with MMP-8 concentrations (r=0.46; p<0.05), also HbA1c levels correlated positively with IL-6 concentrations (r=0.54; p<0.000). Conclusion: Saliva is an adequate substrate for inflammatory biomarkers identification in periodontal patients. IL-6 is a candidate biomarker for periodontitis and periodontitis associated to diabetes in the saliva.