The focus of this dissertation is the use of bioinformatics in the identification of highly conserved sequences among a set of poxvirus genomes and the subsequent functional analysis of the conserved functions of these sequences. A novel algorithm, Java Pattern Finder, which identifies sequences of a user-specified length that are conserved with a user-specified number of allowed differences, was used to identify near-perfectly conserved sequences among a set of poxvirus genomes. A scoring method was established to quantify the degree of conservation of these sequences and used to show that the 11 most conserved sequences were significantly more conserved than control sequences. Functional analysis showed that explanations such as low codon degeneracy or the presence of conserved promoter elements partially – but not fully – accounted for the conservation observed in these sequences, suggesting that these highly conserved regions may have novel functions in the poxvirus genome that have yet to be uncovered.

Submitted in partial compliance with the requirements for the Master of Technology: Chiropractic, Durban University of Technology, 2014. / Background: Poor balance is a risk factor for injury in adolescent sport including soccer. Despite the rapid growth in female adolescent soccer especially in South Africa, the association between balance and injury in this population has not been fully explored. This study aimed to determine the relationship between injury and balance. Static and dynamic balance was monitored as sway index (SI) and limits of stability direction control (LOSDC). Objectives: The objectives of this study were to determine the body mass index of adolescent female soccer players; to determine the prevalence of injury in adolescent female soccer players; to determine static balance as revealed by the sway index (SI); to determine dynamic stability as revealed by limits of stability direction control (LOSDC) and to correlate body mass index (BMI) to sway index and limits of stability. Method: Eighty adolescent female soccer players, between the ages of fourteen and eighteen, were recruited through convenience sampling from schools in the eThekwini district of KwaZulu-Natal. After obtaining informed consent and assent, participants completed questionnaires and were scheduled for the balance and BMI assessments. The objective data for each participant consisted of height, weight, Sway Index (SI) and Limits of Stability Direction Control (LOSDC) readings, measured using a stadiometer, electronic scale and Biodex Biosway Balance System (Biodex Medical Systems Inc., Shirley, New York) respectively. The subjective and objective data were analyzed using SPSS version 21.0 (SPSS Inc. Chicago, Ill, USA). Statistical tests included descriptive statistics using frequency and cross-tabulation. Inferential statistics using t-tests and Pearson’s correlations at a significance level of 0.05 was also incorporated. The testing of hypotheses was performed using Fisher’s Exact tests for nominal data and ordinal data. A p value of < 0.05 was considered as statistically significant. The statistical analysis also included Odds Ratio calculations. Results: The mean body mass index of the injured participants was 23.54±3.56 kg/m2 and the mean body mass index of the uninjured participants was 23.00±4.63. Only 27.5% of the participants sustained an injury. Injured participants performed poorly on average in the SI assessment involving their eyes open when standing on a soft surface. The results were similar for the LOSDC in the overall, right, left, backward-right and backward-left directions. However, there were no significant correlations calculated. Significant relationships existed between BMI and the SI assessments in the injured participants which involved standing on a firm surface with their eyes open (p = 0.05), their eyes closed when also standing on a firm surface (p = 0.05), their eyes open when standing on a soft surface (p = 0.02), and their eyes closed when standing on a soft surface (p = 0.04). A significant relationship also existed between BMI and LOS right direction control (p = 0.02). Conclusion: This research paper revealed that the body mass index as investigated in this study is similar to other studies involving female adolescents; soccer injury as investigated in this study is similar to other studies involving female adolescents; poor static and dynamic balance is not associated with injury in adolescent female soccer players and lastly, body mass index is linked to the balance of an individual.

Research among ministers showed that they are experiencing high levels of stress due to career demands and having to cope with problems on their own (Grosch & Olsen, 2000:260). This has a detrimental effect on their health (Andrew, 1997:SO). Participation in physical activity have positive effects on the physical and psychological well being. Physical activity decrease work stress and increase work satisfaction and -stamina (Dreyer & Strydom, 1992:28). The objectives of this study was to determine the profiles of physical activity participation and the incidence of stress symptoms in relation to congregation type and congregation size in ministers, as well as the influence of physical activity on the relationship between stress symptoms and congregation demography (type and size). Congregation demography was divided into congregation type (location) as well as congregation size (number of church members). In this study a single cross sectional design that has been based on an availability profile has been used. The subjects were 340 ministers of the Dutch Reformed Church, who volunteered to participate in the study. The quality of participation in physical activity was determined with Sharkey's (1997:432) physical activity index The incidence of stress symptoms was determined with Burn's (1988) stress index and the congregation demography was determined by a self-reported questionnaire. The data was analysed by means of a one-way analysis of variance. From the data it is clear that the majority of the respondents were physically inactive and have a low incidence of stress symptoms in relation to congregation demography. The results of the stress symptoms may be attributed to the fact that religion and belief in God may play a vital role in the management of stress. Physical activity participation indicated a statistical significant (p≤0.05) influence on the relationship between stress symptoms and congregation demography. A highly practical significant difference (ES≥0.8) existed between high active and low active ministers working in congregations in rural areas. Physical activity participation also showed a moderate practical significant influence (ES≥0.5) on the relationship between stress symptoms and congregation demography (type and size). It was concluded that physical activity presented a salutogenic effect on psychological well-being among ministers and that congregation demography can influence physical and psycho-emotional health threats of Dutch Reformed ministers. / Thesis (M.A. (Human Movement Science))--North-West University, Potchefstroom Campus, 2005.

A suffix tree is a fundamental data structure for string searching algorithms. Unfortunately, when it comes to the use of suffix trees in real-life applications, the current methods for constructing suffix trees do not scale for large inputs. As suffix trees are larger than their input sequences and quickly outgrow the main memory, the first half of this work is focused on designing a practical algorithm that avoids massive random access to the trees being built. This effort resulted in a new algorithm DiGeST which improves significantly over previous work in reducing random access to the suffix tree and performing only two passes over disk data. As a result, this algorithm scales to larger genomic data than managed before. All the existing practical algorithms perform random access to the input string, thus requiring in essence that the input be small enough to be kept in main memory. The ever increasing amount of genomic data requires however the ability to build suffix trees for much larger strings. In the second half of this work we present another suffix tree construction algorithm, BBST that is able to construct suffix trees for input sequences significantly larger than the size of the available main memory. Both the input string and the suffix tree are kept on disk and the algorithm is designed to avoid multiple random I/Os to both of them. As a proof of concept, we show that BBST allows to build a suffix tree for 12 GB of real DNA sequences in 26 hours on a single machine with 2 GB of RAM. This input is four times the size of the Human Genome. The construction of suffix trees for inputs of such magnitude was never reported before. Finally, we show that, after the off-line suffix tree construction is complete, search queries on entire sequenced genomes can be performed very efficiently. This high query performance is achieved due to a special disk layout of the suffix trees produced by our algorithms.

We construct geometric models for K-homology with coefficients based on the theory of Z/k-manifolds. To do so, we generalize the operations and relations Baum and Douglas put on spinc-manifolds to spinc Z/kZ-manifolds. We then de fine a model for K-homology with coefficients in Z/k using cycles of the form ((Q,P), (E,F), f) where (Q, P) is a spinc Z/k-manifold, (E, F) is a Z/k-vector bundle over (Q, P) and f is a continuous map from (Q, P) into the space whose K-homology we are modelling. Using results of Rosenberg and Schochet, we then construct an analytic model for K-homology with coefficients in Z/k and a natural map from our geometric model to this analytic model. We show that this map is an isomorphism in the case of finite CW-complexes. Finally, using direct limits, we produced geometric models for K-homology with coefficients in any countable abelian group.

The purpose of this thesis is to construct an index of adolescent health and wellness for British Columbia, Canada, using the most recent available data. A three- round Delphi study is used in order to decide on what indicators to include in the index and each indicator’s relative weight. Spatial multi-criteria analysis (MCA) is utilized to combine the indicators into a single measure. The spatial MCA, technique for order preference by similarity to an ideal solution (TOPSIS) method was applied to the adolescent population as a whole and to examine male and female variation. This revealed that adolescent health and wellness is not experienced equally across the province. The Health Service Delivery Areas (HSDAs) Fraser South and Fraser North proved to have the greatest levels of adolescent health and wellness while the Northwest has the least. A rural/ urban gradient in adolescent health and wellness was revealed at the HSDA level. Male and female adolescents also experience health and wellness differently, with females achieving higher health and wellness across all HSDAs in the Province when directly comparing the two genders. The findings of this research are useful in informing discussions of resource allocation for reducing inequalities and inequities and in order to target future research.

The Atiyah-Singer index theorem, the Euler number, and the Hirzebruch signature are derived via the supersymmetric path integral. Concisely, the supersymmetric path integral is a combination of a bosonic and a femionic path integral. The action in the supersymmetric path integral includes here bosonic, fermionic- and isospin fields (backgroundfields), where the cross terms in the Lagrangian are nicely eliminated due to scaling of the fields and using techniques from spontaneous breaking of supersymmetry (that give rise to a mechanism, analogous to the Higgs-mechanism, but here regarding the so called superparticles instead).  Thus, the supersymmetric path integral is a product of three pathintegrals over the three given fields, respectively, that can be evaluated exactly by means of Gaussian integrals. The closely related Witten index is a measure of the failure of spontaneous breaking of supersymmetry. In addition, the basic concepts of supersymmetry breaking are reviewed.

Tourism is one of the largest economic sectors globally. It is a climate sensitive sector, with climate being one of the most important attributes for a destination. The Tourism Climate Index (TCI), developed by Mieczkowski (1985), is the most widely used index for assessing a destination’s climatic suitability for general tourist activities. Major deficiencies such as the subjectivity of its rating system and component weightings have been identified in the literature, and the need to develop a new index has been identified by researchers for almost a decade. This study aims to fill the research gap by developing a new index, the Holiday Climate Index (HCI), for the purpose of overcoming the deficiencies of the TCI. The HCI was compared with the TCI in rating both current (1961-1990) and future (2010-2039, 2040-2069 and 2070-2099) climatic suitability for tourism of the 15 most visited European city destinations (London, Paris, Istanbul, Rome, Barcelona, Dublin, Amsterdam, Vienna, Madrid, Berlin, Stockholm, Warsaw, Munich, Athens and Venice). The results were also compared with monthly visitation data available for Paris to assess whether the HCI ratings more accurately represent visitation demand than the TCI. The results show that there are key differences between the HCI and TCI in rating the tourism climate suitability of the selected European city destinations, in particular in the winter months of the northern, western and eastern European city destinations where the performance of the TCI had been questioned in the literature. The comparison with leisure tourist visitation data in Paris also revealed that the ratings of the HCI were more reflective of seasonal pattern of tourist arrivals than the TCI ratings. Because the TCI has been widely applied (15 studies), these findings hold important implications for future research in assessing current and future climatic suitability for tourism.

dissertation contains seven papers. The first five are in the field of real estate finance, while the final two are asset pricing papers. The first paper explores equilibrium properties of lease rates. The framework is based on the concept of the term structure of lease rates, or the equilibrium rate as a function of lease length. In particular the analysis sheds light on the relationship between objective rental expectations and forward lease rates. The second, third and fourth papers in the dissertation consider the pricing of explicit real estate contracts. More specifically a type of indexed leases subject to a floor rate, leases with embedded renewal options and a real estate swap contract are analyzed and priced. The fifth paper considers the impact of index revision in the context of home equity insurance schemes. The idea of establishing markets allowing homeowners to hedge housing risk has been given significant attention in recent years. If contract settlement is based on a house price index, it is important to understand the scope of index revision, or the updating of earlier index estimates due to the arrival of new information. Using a rich data set, comprising all arm’s length housing transaction in Sweden during the period 1981-99, the revision properties of hedonic and repeat sales indexes are explored. The final two papers are in the field of asset pricing. The sixth paper revisits the consumption based asset pricing model using Swedish data. The seventh paper is a Monto Carlo study on the properties of different measures of misspecification under varying assumptions of asset return distributions. / Diss. Stockholm : Handelshögskolan, 2005 S. 3-9: sammanfattning, s. 11-177: 7 uppsatser

Univ., Diss--Gießen, 2000.