Aujourd’hui, le marché des services est devenu de plus en plus concurrentiel. Les exigences des clients pour des offres de service en adéquation avec leurs usages et leurs préférences conduisent les fournisseurs à proposer de nouveaux services qui répondent à ce nouveau besoin pour se démarquer des concurrents et attirer de nouveaux clients. Avec la convergence des réseaux et celle des services de nouvelle génération (NGN/NGS), de nouveaux services sont apparus. Les utilisateurs sont nomades et veulent utiliser leurs services de différentes manières n’importe où, n’importe quand et par n’importe quel type de terminal, et cela avec une continuité de service et une qualité de service de bout en bout. Ainsi, fournir des services personnalisés aux clients dans un environnement hétérogène et mobile devient un challenge pour les opérateurs et les fournisseurs de service pour améliorer le retour sur investissement (ROI) et le délai de mise sur le marché (TTM). Nos réflexions à propos de la fourniture des services personnalisés selon les besoins fonctionnels et non-fonctionnels (QoS) des usagers, nous ont conduits à identifier les besoins du nouveau contexte NGN/NGS défini par l’intersection de ces trois éléments «user-centric, mobilité et QoS». Comment piloter dynamiquement la QoS de bout en bout pour une session unique «user-centric»? Comment assurer le « service Delivery» dans un contexte de mobilité et d’ubiquité? Ces nouveaux besoins, nous ont motivé à proposer des solutions à travers trois contributions principales qui prennent en considération la vision utilisateur et opérateur. Notre première contribution porte sur le modèle organisationnel. Nous proposons une nouvelle organisation avec un maximum de flexibilité, d’adaptabilité et d’autogestion, qui permet de piloter la QoS à chaque niveau de l’architecture (équipement, réseau et service). Dans cette organisation nous avons défini des acteurs et le rôle que joue chacun d’eux par rapport à la prise de décision au cours de la session de l’utilisateur, et cela pour maintenir la QoS de bout en bout dans un environnement qui est totalement hétérogène et mobile.Notre deuxième contribution traite du composant de service autonomique. Avec la complexité de la personnalisation des services dans un contexte hétérogène et mobile et le besoin de satisfaire la QoS de bout en bout, les ressources services doivent être prises en compte au même titre que les ressources réseaux. Donc, un degré élevé d’autosuffisance, d’autogestion et d’automatisation est demandé dans la ressource service (composant de service) pour améliorer le service delivery. Pour cela, nous proposons un composant de service autonomique «ASC: Autonomic Service Component» basé sur un agent de QoS intégré qui s’autocontrôle et s’autogère pour adapter dynamiquement ses ressources en réponse à un changement de situations au cours de la session de l’utilisateur. Notre troisième proposition couvre le modèle protocolaire. La session de services personnalisés nécessite des interactions plus flexibles au niveau service pour avoir une session unique avec une continuité de service. Nous proposons un protocole de signalisation SIP+ qui permet la négociation de la QOS des services personnalisés dès la phase d’initialisation de la session et de la renégociation de la QoS pendant l’usage, pour maintenir le service avec la QoS requise à travers une session unique.De façon plus concrète, nous présentons nos expérimentations à travers un scenario et une plate-forme de démonstration qui nous permet de tester la faisabilité et la performance de nos contributions. Les apports et les perspectives de cette thèse sont consignés en conclusion. / Nowadays, the services market has become increasingly competitive. Customer requirements for service offerings in line with their uses and preferences led providers to offer new services to meet this new need and to stand out from competitors and attract new customers. With the success of the network and service convergence (NGN / NGS), new services have emerged. A mobile user desires to access his services anywhere, anytime and on any type of terminal.Thus, providing customized services to clients while ensuring the service continuity and the end-to-end quality of service in a heterogeneous and mobile environment became a challenge for mobile operators and service providers to improve the return on investment (ROI) and time-to-market (TTM). Our thinking about the provision of customized services according to the functional and non-functional (QoS) needs of the users has led us to identify the needs of the new context NGN / NGS defined by the intersection of these three elements "user-centric, mobility and QoS". How to dynamically control the end-to-end QoS for a single "user-centric" session? How to ensure the "Service Delivery" in the context of mobility and ubiquity? These new needs have led us to propose solutions through three main contributions that take into account the user and the operator vision. Our first contribution concerns the organizational model. We have proposed a new organization with a maximum of flexibility, adaptability and self-management which allows the control of the QoS at each level of the architecture (equipment, network and service). In this organization, we have defined actors and the role of each one in relation to the decision-making process during the user session in order to maintain the end-to-end QoS in an environment that is totally heterogeneous and mobile. Our second contribution addresses the autonomic service component. With the complexity of services personalization in a heterogeneous and mobile context and the need to satisfy the end to end QoS, services and network resources must be taken into account. Therefore, a high degree of self-sufficiency, self-management and automation is required in the resource service to improve the service delivery. We have therefore proposed an autonomic service component based on an integrated QoS-agent which is self-controlled and self-managed to dynamically adapt its resources in response to changing situations during the user’s session. Our third proposal covers the model protocol. The personalized services session requires more flexible interactions at the service level in order to obtain a single session with service continuity. We have proposed a signalling protocol SIP + that allows the negotiation of the QoS of personalized services at the session initialization phase and the renegotiation of the QoS during the utilization to maintain the service with the required QoS through a unique session. More concretely, we have presented our experiments through a scenario and demonstration platform that allows us to test the feasibility and the performance of our contributions. The contributions and perspectives of this thesis are stated in the conclusion.

[pt] A pesquisa traz reflexões acerca da chamada Nova Geração do samba no Rio de Janeiro, destacada, especialmente, pelas rodas de samba no Beco do Rato, bar localizado entre os bairros da Glória e da Lapa, e também a partir da elaboração do DVD Samba Social Clube - Nova geração, Vol 1., em 2016, e o DVD Samba Social Clube - Nova geração, Vol 2., lançado em 2020. Deste modo, aprofunda-se o debate pelo vetor da inserção, dessa categoria, na cena musical, a fim de compreender suas sonoridades, repertório, as rotinas de trabalho e a relação com as demandas sociais contemporâneas como machismo, racismo e homofobia. Por fim, analisa-se como o apadrinhamento está presente na trajetória desses músicos, explorando as relações e parcerias com nomes célebres do samba, a herança familiar e outros sentidos possíveis nestas articulações. / [en] The research brings reflections on the so-called New Generation of samba in Rio de Janeiro, highlighted, especially, by the wheels of samba in the Alley of the Mouse, bar located between the neighborhoods of Gloria and Lapa, and also from the elaboration of the DVD Samba Social Club - New generation, Vol 1., in 2016, and the DVD Samba Social Club - New generation, Vol 2., released in 2020. In this way, the debate is deepened by the vector of the inclusion of this category in the musical scene, in order to understand its sonorities, repertoire, work routines and the relationship with contemporary social demands such as machismo, racism and homophobia. Finally, it is analyzed how the sponsorship is present in the trajectory of these musicians, exploring the relationships and partnerships with famous names of the samba, the family heritage and other possible senses in these joints.

A miopatia miotubular é uma doença genética congênita que afeta a musculatura esquelética e respiratória, causada por mutações no gene MTM1. Apresenta padrão de herança recessivo ligado ao cromossomo X e frequência estimada de 1/50.000 meninos nascidos vivos. O diagnóstico é geralmente realizado através de biopsia muscular, com presença de fibras pequenas com núcleo central, predominância de fibras do tipo I, concentração de miofibrilas na periferia da fibra e região central ocupada por acúmulos de mitocôndrias e glicogênio. O quadro clínico é bastante grave, com manifestação clínica no período neonatal e óbito nos primeiros meses, ou ano de vida. Os pacientes apresentam hipotonia e fraqueza muscular generalizadas, dificuldade de alimentação, ptose palpebral, oftalmoplegia, hérnia inguinal e criptorquidia. Mulheres portadoras das mutações são geralmente assintomáticas, mas diversos casos de heterozigotas sintomáticas têm sido relatados. Pacientes com miopatias congênitas estruturais vem sendo estudados nos últimos 20 anos no Centro de Pesquisa do Genoma Humano e Células Tronco (CPGH-CEL) da Universidade de São Paulo (USP). Atualmente, em razão do avanço das tecnologias de análise molecular do DNA, como o sequenciamento de nova geração (NGS - Next Generation Sequencing), o diagnóstico tem se tornado cada vez mais preciso. No presente trabalho, pacientes de 12 famílias estudadas no CEGH-CEL foram submetidos à triagem mutacional, utilizando técnica de NGS. Onze mutações foram identificadas (c.109 C>T; c.139_142 delAAAG; c.706 A>T; c.1010 G>A, c.1181 A>G, c.1262 G>A, c.1354 -1 G>C, c.1465_1465delC, c.1467 +1 G>A, c.1528 A>T; c.1528 A>T); entre elas 5 já descritas como patogênicas e 6 são novas. Em duas famílias, foram identificadas 4/8 e 2/4 mulheres portadoras apresentando algum nível de manifestação clínica. A análise de desvio de inativação do X revelou desvio aleatório em pelo menos 4 das heterozigotas manifestantes. Além disso, adicionando os casos deste trabalho aos relatados na literatura, a taxa de penetrância da doença foi estimada em 30% em mulheres heterozigotas, o que é compatível com um padrão de penetrância incompleta e poderia explicar a alta frequência de mulheres manifestantes. Uma análise de exomas foi realizada a fim de identificar possíveis genes modificadores que explicassem a variabilidade clínica observada. Foi identificada uma região de 4,2 Mb contendo genes contíguos no cromossomo 19 que pode estar relacionado à modulação do fenótipo / Myotubular myopathy is a rare congenital muscle genetic disease, caused by mutations in the MTM1 gene. With a X-linked recessive inheritance, the disease affects 1/50.000 living born males. The clinical picture is characteristic and very severe, with manifestation in the neonatal period, including generalized hypotonia and muscle weakness, feeding difficulty, palpebral ptosis, ophthalmoplegia, inguinal hernia, and cryptorchidism. Most affected die in the first few months or year of life, and those who survive often depend on care and assistance to perform activities of daily living, as well as require mechanical ventilation and enteral nutrition. Females carrying the mutations are generally asymptomatic, but several cases of symptomatic heterozygotes have been reported, compared to the low frequency of manifesting carriers in other X-recessive diseases. Patients with structural congenital myopathies have been studied in the last 20 years at the Human Genome and Stem Cell Research Center (HUG-CELL) at the University of São Paulo (USP). The diagnosis of myotubular myopathy is usually made with muscle biopsy findings, with small fibers with central nuclei, the predominance of type I fibers, the concentration of myofibrils in the periphery of the fiber and central region occupied by accumulations of mitochondria and glycogen. More recently, with the advancement of DNA molecular analysis technologies, such as Next Generation Sequencing (NGS), the diagnosis has become increasingly accurate. In the present study, patients from 12 families studied in the HUG-CEL were submitted to mutation screening using NGS techniques. Eleven mutations were identified (c.109 C> T; c.139_142 delAAAG; c.706 A> T; c.1010 G> A, c.1181 A> G, c.1262 G> A, c.1354-1 G> C, c.1465_1465delC, c.1467 +1 G> A, c.1528 A> T; c.1528 A> T); among them 6 are novel. In two families, 4/8 and 2/4 female carriers were identified, presenting some level of clinical manifestation. Inactivation skewing analysis of the X chromosome revealed random inactivation in at least 4 of the manifesting carriers. In addition, joining the cases of this work to those reported in the literature, the disease penetrance rate was estimated to be 30% in heterozygous women, which is compatible with an incomplete penetrance pattern and could explain the high frequency of manifesting females. An exome analysis was performed to identify possible modifying genes that explain the observed clinical variability. A region of 4,2 Mb containing contiguous genes was identified on chromosome 19 that may be related to phenotype modulation

O manejo agrícola aplicado a um agrossistema pode determinar a qualidade e produtividade da área, além das interações biológicas que podem ser estabelecidas entre o cultivar e ecossistema local. A agricultura convencional é bastante reconhecida como um manejo eficiente e lucrativo. Por outro lado, a agroecologia tem ganhado visibilidade na agroindústria em reflexo do aumento na demanda por alternativas mais sustentáveis de produção. As diferenças entre ambos manejos podem refletir sobre a dinâmica microbiana, alterando a composição e estruturação das comunidades ali presentes. Os micro-organismos que habitam a superfície foliar da planta compõem o micro-ambiente denominado filosfera, descrito como um dos hábitats colonizáveis mais extensos. Devido a sua alta exposição a variáveis ambientais, diversos fatores podem interferir na comunidade bacteriana e definir a filosfera. Desta forma, o principal objetivo nesse estudo foi avaliar como o manejo agrícola interfere na composição bacteriana na filosfera, analisando ainda em escala temporal sua estrutura e abundância. A área experimental amostrada foi cedida pelo Centro de Pesquisa \"Mokiti Okada\", em Mogi Guaçu, São Paulo. As amostras foram coletadas de maneira representativa em diferentes linhas de tratamento, uma sob manejo convencional e outra sob manejo ecológico. Análises microbiológicas dependentes e independentes de cultivo permitiram identificar a comunidade bacteriana residente da filosfera de citros, a qual era compartilhada por ambos os manejos. Entretanto, análises de sequenciamento NGS (New Generation Sequencing) mostraram uma diferença significativa entre as comunidades bacterianas dos dois manejos, com o ecológico apresentando uma maior diversidade. Apesar do manejo ter se mostrado um importante fator na composição bacteriana, quando avaliado em função temporal, viu-se que as épocas de coleta interferem mais intensamente na estrutura das bactérias (p=0,0001), mostrando uma sobreposição dos diversos fatores ambientais que atuam sobre a filosfera. Os resultados ainda indicaram uma redução na abundância de bactérias, a qual pode estar relacionada com a aplicação extra de produtos cúpricos em ambas as áreas, em função do acometimento da \"pinta preta\" no pomar, o que instigou monitorar o cobre no tecido foliar. Quimicamente, micro-análises de XRF (X-Ray Fluorescence) mostraram que há uma maior concentração de cobre nas folhas provenientes da área convencional, o que é resultado das maiores quantidades do produto que são aplicadas nesse tratamento. Além disso, foi possível o isolamento de bactérias do gênero Enterococcus na filosfera, as quais apresentam mecanismos de tolerância ao cobre, demonstrando que os produtos cúpricos podem ter selecionado esses organismos. Logo, esse estudo apresentou uma importante perspectiva do efeito do manejo agrícola sobre a filosfera, contribuindo para a compreensão da dinâmica microbiana na agricultura. / The agricultural management applied to a agrosystem is an important determinant for the quality and productivity of the crop yield, also for the biological interactions that can be stablished between the plants and the local ecosystem. Conventional agriculture has being well known as an efficient and lucrative crop management. On the other hand, agroecology has gaining visibility in the agroindustry due to increasing demand for a more sustainable production alternative. The differences between both approaches can reflect on the microbial dynamic, affecting the composition and structure of these communities. Microrganisms inhabitating the foliar surface correspond to a microenvironment called phyllosphere, which is described as one of the most extensive habitats. Due to its constant exposition to environmental variables, several factors can influence on the bacterial community and modulate the phyllosphere. Thus, the main purpose of this study was to evaluate how the agricultural management can impact on the phyllospheric bacteria, also considering a temporal effect on structure and abundance of these organisms. The experimental area was provided by \"Mokiti Okada\" Research Center, located at em Mogi-Guaçu, São Paulo. The samples were representativelly collected from two treatment lines, one under convencional management, and the other under ecological management. Afterwards, culture-dependent and independent microbiological analysis allowed to identify the resident bacterial community in citros phyllosphere, which was greatly shared bewteen both treatments. However, NSG (New Generation Sequencing) analysis demonstrated a significative difference between the bacterial community under conventional and ecological management, where the second one demonstrated a higher diversity, which can be related to the different approaches applied. Although the agricultural method have demonstrated an important factor on bacterial composition, when temporally evaluated, it was observed a more intense interferance on the bacterial structure by the time of sampling (p=0,0001), representing a possible overlap of environmental factors on the phyllosphere. The data also indicate a decrease in the abundance of bacteria that might be resulted from the extra use of cupric products, related to the impairment of \"black spot\" on the crop, what lead to a copper monitoring in the foliar tissue. Chemically, XRF micro-analysis (X-Ray Fluorescence) demonstrated that there is a higher concentration of copper on the leaves from the conventional area, which is resulted of the higher application of its products by this method. Moreover, a search for copper-tolerant microrganisms was conducted, and it was possible to isolate Enterococcus bacteria, which have copper tolerance mechanisms. This result implicate that the use of cupric products may have selected these microrganisms on citros phyllosphere. Therefore, this study presented an important perspective of how the agricultural management can influence the phyllosfere, which can contribute to undertand about the microbial dynamic and its roles on the agriculture.

A primeira reação nuclear em cadeia autosustentada controlada foi obtida em 2 de dezembro de 1942. Daí em diante, o crescimento da energia nuclear, inicialmente estimulado por fins militares, foi rápido. Ás aplicações civis no setor da geração de eletricidade foram adquirindo, ao longo do tempo, um papel sempre mais importante nas matrizes energéticas de muitos paises. Em 1987, 418 reatores nucleares no mundo estavam produzindo eletricidade em escala comercial. Dois terços destes reatores eram localizados em 7 países: Estados Unidos, União Soviética, França, Reino Unido, Alemanha, Canadá e Japão. Nos anos 90, o setor nuclear experimentou um grande retardo, devido principalmente ao acidente de Chernobyl e a uma revisão otimista das perspectivas de esgotamento das reservas de petróleo e dos outros combustíveis fosseis. Em 2005 o número de reatores para geração de eletricidade em operação no mundo era de 441, não muito diferente do numero de reatores em operação em 1987. Neste panorama o primeiro objetivo deste trabalho é analisar o estado da arte da geração núcleo elétrica e do ciclo do combustível nos países acima mencionados, partindo de uma revisão histórica. O caso do Brasil é abordado também por ser o país onde este trabalho é desenvolvido. Uma vez concluído o quadro da geração núcleo elétrica a nível internacional, são analisadas as novas tecnologias no setor da geração núcleo elétrica e as tendências e as iniciativas para o futuro da utilização da energia nuclear. São também abordadas as principais questões que sempre acompanharam o debate sobre a energia nuclear: a segurança, o meio ambiente, a proliferação e o mais moderno conceito de desenvolvimento sustentável. É importante antecipar que o objetivo deste trabalho não é de julgar os acontecimentos e de influenciar a opinião do leitor a favor da energia nuclear, mas de selecionar materiais e dados para informar e assim fornecendo um texto que seja uma coleção de informações e sugestões de aprofundamentos e não uma fonte de polêmicas. / On December 2, 1942, man first initiated a self-sustaining nuclear chain reaction, and controlled it. Since then, nuclear energy development, firstly estimulated by military goals, was fast. But nuclear energy use for electricity production grew too, until becoming a very important energy source in the world energy mix. In 1987 there were in the world 418 nuclear reactors capable of producing commercially useful supplies of electricity. Over two thirds were in just seven countries: United States, Soviet Union, France, United Kingdom, Germany, Canada and Japan. In the 90s, nuclear energy development slowed down as a consequence of the Chernobyl accident and of the more optimistic evaluations of world oil resources. In 2005 the number of nuclear reactors commercially producing electricity amounted to 441, not much more than the 418 reactors operating in 1987. From this point of view, the primary scope of this work is to analyze the world pattern and the state of the art of nuclear power production focusing on the countries above mentioned. Brazil case is analyzed too, since this work has been developed there. Once this international outlook is concluded, the next step passes through the analyses of new technologies, tendencies and initiatives for the future development of nuclear energy. Since feelings run high in the debate about nuclear energy, some fundamental and fervent points are raised: security, environment, proliferation and sustainable development. Nevertheless, it is important to point out that effort has been made in this work not to take sides, but to be impartial in selecting materials and giving data. The scope is not to convert the reader to a pro-nuclear view but to inform and, in doing so, to provide a volume that is a textbook and not a piece of polemic.

A primeira reação nuclear em cadeia autosustentada controlada foi obtida em 2 de dezembro de 1942. Daí em diante, o crescimento da energia nuclear, inicialmente estimulado por fins militares, foi rápido. Ás aplicações civis no setor da geração de eletricidade foram adquirindo, ao longo do tempo, um papel sempre mais importante nas matrizes energéticas de muitos paises. Em 1987, 418 reatores nucleares no mundo estavam produzindo eletricidade em escala comercial. Dois terços destes reatores eram localizados em 7 países: Estados Unidos, União Soviética, França, Reino Unido, Alemanha, Canadá e Japão. Nos anos 90, o setor nuclear experimentou um grande retardo, devido principalmente ao acidente de Chernobyl e a uma revisão otimista das perspectivas de esgotamento das reservas de petróleo e dos outros combustíveis fosseis. Em 2005 o número de reatores para geração de eletricidade em operação no mundo era de 441, não muito diferente do numero de reatores em operação em 1987. Neste panorama o primeiro objetivo deste trabalho é analisar o estado da arte da geração núcleo elétrica e do ciclo do combustível nos países acima mencionados, partindo de uma revisão histórica. O caso do Brasil é abordado também por ser o país onde este trabalho é desenvolvido. Uma vez concluído o quadro da geração núcleo elétrica a nível internacional, são analisadas as novas tecnologias no setor da geração núcleo elétrica e as tendências e as iniciativas para o futuro da utilização da energia nuclear. São também abordadas as principais questões que sempre acompanharam o debate sobre a energia nuclear: a segurança, o meio ambiente, a proliferação e o mais moderno conceito de desenvolvimento sustentável. É importante antecipar que o objetivo deste trabalho não é de julgar os acontecimentos e de influenciar a opinião do leitor a favor da energia nuclear, mas de selecionar materiais e dados para informar e assim fornecendo um texto que seja uma coleção de informações e sugestões de aprofundamentos e não uma fonte de polêmicas. / On December 2, 1942, man first initiated a self-sustaining nuclear chain reaction, and controlled it. Since then, nuclear energy development, firstly estimulated by military goals, was fast. But nuclear energy use for electricity production grew too, until becoming a very important energy source in the world energy mix. In 1987 there were in the world 418 nuclear reactors capable of producing commercially useful supplies of electricity. Over two thirds were in just seven countries: United States, Soviet Union, France, United Kingdom, Germany, Canada and Japan. In the 90s, nuclear energy development slowed down as a consequence of the Chernobyl accident and of the more optimistic evaluations of world oil resources. In 2005 the number of nuclear reactors commercially producing electricity amounted to 441, not much more than the 418 reactors operating in 1987. From this point of view, the primary scope of this work is to analyze the world pattern and the state of the art of nuclear power production focusing on the countries above mentioned. Brazil case is analyzed too, since this work has been developed there. Once this international outlook is concluded, the next step passes through the analyses of new technologies, tendencies and initiatives for the future development of nuclear energy. Since feelings run high in the debate about nuclear energy, some fundamental and fervent points are raised: security, environment, proliferation and sustainable development. Nevertheless, it is important to point out that effort has been made in this work not to take sides, but to be impartial in selecting materials and giving data. The scope is not to convert the reader to a pro-nuclear view but to inform and, in doing so, to provide a volume that is a textbook and not a piece of polemic.

O manejo agrícola aplicado a um agrossistema pode determinar a qualidade e produtividade da área, além das interações biológicas que podem ser estabelecidas entre o cultivar e ecossistema local. A agricultura convencional é bastante reconhecida como um manejo eficiente e lucrativo. Por outro lado, a agroecologia tem ganhado visibilidade na agroindústria em reflexo do aumento na demanda por alternativas mais sustentáveis de produção. As diferenças entre ambos manejos podem refletir sobre a dinâmica microbiana, alterando a composição e estruturação das comunidades ali presentes. Os micro-organismos que habitam a superfície foliar da planta compõem o micro-ambiente denominado filosfera, descrito como um dos hábitats colonizáveis mais extensos. Devido a sua alta exposição a variáveis ambientais, diversos fatores podem interferir na comunidade bacteriana e definir a filosfera. Desta forma, o principal objetivo nesse estudo foi avaliar como o manejo agrícola interfere na composição bacteriana na filosfera, analisando ainda em escala temporal sua estrutura e abundância. A área experimental amostrada foi cedida pelo Centro de Pesquisa \"Mokiti Okada\", em Mogi Guaçu, São Paulo. As amostras foram coletadas de maneira representativa em diferentes linhas de tratamento, uma sob manejo convencional e outra sob manejo ecológico. Análises microbiológicas dependentes e independentes de cultivo permitiram identificar a comunidade bacteriana residente da filosfera de citros, a qual era compartilhada por ambos os manejos. Entretanto, análises de sequenciamento NGS (New Generation Sequencing) mostraram uma diferença significativa entre as comunidades bacterianas dos dois manejos, com o ecológico apresentando uma maior diversidade. Apesar do manejo ter se mostrado um importante fator na composição bacteriana, quando avaliado em função temporal, viu-se que as épocas de coleta interferem mais intensamente na estrutura das bactérias (p=0,0001), mostrando uma sobreposição dos diversos fatores ambientais que atuam sobre a filosfera. Os resultados ainda indicaram uma redução na abundância de bactérias, a qual pode estar relacionada com a aplicação extra de produtos cúpricos em ambas as áreas, em função do acometimento da \"pinta preta\" no pomar, o que instigou monitorar o cobre no tecido foliar. Quimicamente, micro-análises de XRF (X-Ray Fluorescence) mostraram que há uma maior concentração de cobre nas folhas provenientes da área convencional, o que é resultado das maiores quantidades do produto que são aplicadas nesse tratamento. Além disso, foi possível o isolamento de bactérias do gênero Enterococcus na filosfera, as quais apresentam mecanismos de tolerância ao cobre, demonstrando que os produtos cúpricos podem ter selecionado esses organismos. Logo, esse estudo apresentou uma importante perspectiva do efeito do manejo agrícola sobre a filosfera, contribuindo para a compreensão da dinâmica microbiana na agricultura. / The agricultural management applied to a agrosystem is an important determinant for the quality and productivity of the crop yield, also for the biological interactions that can be stablished between the plants and the local ecosystem. Conventional agriculture has being well known as an efficient and lucrative crop management. On the other hand, agroecology has gaining visibility in the agroindustry due to increasing demand for a more sustainable production alternative. The differences between both approaches can reflect on the microbial dynamic, affecting the composition and structure of these communities. Microrganisms inhabitating the foliar surface correspond to a microenvironment called phyllosphere, which is described as one of the most extensive habitats. Due to its constant exposition to environmental variables, several factors can influence on the bacterial community and modulate the phyllosphere. Thus, the main purpose of this study was to evaluate how the agricultural management can impact on the phyllospheric bacteria, also considering a temporal effect on structure and abundance of these organisms. The experimental area was provided by \"Mokiti Okada\" Research Center, located at em Mogi-Guaçu, São Paulo. The samples were representativelly collected from two treatment lines, one under convencional management, and the other under ecological management. Afterwards, culture-dependent and independent microbiological analysis allowed to identify the resident bacterial community in citros phyllosphere, which was greatly shared bewteen both treatments. However, NSG (New Generation Sequencing) analysis demonstrated a significative difference between the bacterial community under conventional and ecological management, where the second one demonstrated a higher diversity, which can be related to the different approaches applied. Although the agricultural method have demonstrated an important factor on bacterial composition, when temporally evaluated, it was observed a more intense interferance on the bacterial structure by the time of sampling (p=0,0001), representing a possible overlap of environmental factors on the phyllosphere. The data also indicate a decrease in the abundance of bacteria that might be resulted from the extra use of cupric products, related to the impairment of \"black spot\" on the crop, what lead to a copper monitoring in the foliar tissue. Chemically, XRF micro-analysis (X-Ray Fluorescence) demonstrated that there is a higher concentration of copper on the leaves from the conventional area, which is resulted of the higher application of its products by this method. Moreover, a search for copper-tolerant microrganisms was conducted, and it was possible to isolate Enterococcus bacteria, which have copper tolerance mechanisms. This result implicate that the use of cupric products may have selected these microrganisms on citros phyllosphere. Therefore, this study presented an important perspective of how the agricultural management can influence the phyllosfere, which can contribute to undertand about the microbial dynamic and its roles on the agriculture.

The Portfolio Analysis Management Office (PAMO) for the Aeronautics Research Mission Directorate (ARMD) at NASA Headquarters tasked the Systems Analysis and Concepts Directorate at NASA Langley to combine efforts with Virginia Tech to develop a global demand model with the capability to predict future demand in the air transportation field. A previous study (Alsalous, 2015) started the development of the Global Demand Mode (GDM) to predict air travel demand based on Gross Domestic Product (GDP) and population trends for 3,974 airports worldwide. The study was done from year 2016 to year 2040. This research project intends to enhance the GDM capabilities. A Fratar model is implemented for the distribution of the forecast demand during each year. The Fratar model uses a 3,974 by 3,974 origin-destination matrix to distribute the demand among 55,612 unique routes in the network. Moreover, the GDM is capable to estimate the aircraft fleet mix per route and the number of flights per aircraft that are needed to satisfy the forecast demand. The model adopts the aircraft fleet mix from the Official Airline Guide data for the year 2015. Once the aircraft types are distributed and flights are assigned, the GDM runs an aircraft retirement and replacement analysis to remove older generation aircraft from the network and replace them with existing or newer aircraft. The GDM continues to evolve worldwide aircraft fleet by introducing 14 new generation aircraft from Airbus, Boeing, Bombardier, and Embraer and 5 Advanced Technology Aircraft from NASA. / Master of Science / The Portfolio Analysis Management Office (PAMO) for the Aeronautics Research Mission Directorate (ARMD) at NASA Headquarters tasked the Systems Analysis and Concepts Directorate at NASA Langley to combine efforts with Virginia Tech to develop a global demand model with the capability to predict future demand in the air transportation field. A previous study (Alsalous, 2015) started the development of the Global Demand Mode (GDM) to predict air travel demand based on Gross Domestic Product (GDP) and population trends for 3,974 airports worldwide. The study was done from year 2016 to year 2040. The previous study done by Alsaous, predicts how many seats will be departing out of the 3,974 airports worldwide. This project intends to use the outputs of the GDM and distribute the seats predicted among the airports. The objective is to predict how many seats will be offered that will be departing from airport “A” and arriving at airport “B”. For this, a Fratar model was implemented. The second objective of this project is to estimate what will the aircraft fleet be in the future and how many flights will be needed to satisfy the predicted air travel demand. If the number of seats going from airport A to airport B is known, then, by analyzing real data it can be estimated what type of aircraft will be flying from airport “A” to airport “B” and how many flights each aircraft will have to perform in order to satisfy the forecasted demand. Besides of estimating the type of aircraft that will be used in the future, the modeled created is capable of introducing new aircraft that are not part of the network yet. Fourteen new generation aircraft from Airbus, Boeing, Bombardier, and Embraer and 5 Advanced Technology Aircraft from NASA.

This thesis discusses enhancements to the Global Demand Model (GDM). The model addresses the need to predict: a) number of flights Worldwide by Origin-Destination (OD) airport pair, b) the number of seats (surrogate of demand) by OD airport pair, c) the fleet evolution over time, d) fuel consumption by OD pair and aircraft type, and emissions by OD pair and aircraft type. The model has developed an airline fleet assignment module to predict changes to the airline fleet in the future. Specifically, the model has the capability to examine the fuel and emission benefits of next generation N+1 aircraft and advanced NASA's N+2 aircraft are adopted in the future. / Master of Science / This thesis discusses enhancements to a model, Global Demand Model (GDM), developed at Air Transportation Systems Laboratory at Virginia Tech. The model addresses the need to predict: a) number of flights Worldwide by Origin-Destination (OD) airport pair, b) the number of seats (surrogate of demand) by OD airport pair, c) the fleet evolution over time, d) fuel consumption by OD pair and aircraft type, and emissions by OD pair and aircraft type. The model has developed an airline fleet assignment module to predict changes to the airline fleet in the future. Specifically, the model has the capability to examine the fuel and emission benefits if next generation N+1 aircraft and advanced NASA’s N+2 aircraft are adopted in the future.

Compreender a ecologia microbiana durante a ensilagem é fundamental para neutralizar pontos críticos relacionados à produção de silagens de qualidade por meio da prevenção do crescimento de bactérias oportunistas que possam comprometer a segurança da cadeia alimentar animal e o rendimento da forragem ensilada. Ensilar GSR de milho possibilita a compra estratégica em momentos de baixa nos preços do grão. O objetivo deste estudo foi avaliar por meio de sequenciamento massivo do gene 16S rRNA o efeito da reconstituição da umidade do grão de milho na dinâmica da comunidade bacteriana para confecção dessa silagem. Foram amostrados milhos plantados em dois distintos locais. As plantas usadas no estudo incluíram dois híbridos contrastantes, \"dent\" (AG 1051) e outro \"flint\" (IAC 8390) ensilados de duas maneiras: grão úmido (GU), com a umidade original e grãos secos reconstituídos (GSR) à 30 % U, ambos ensilados por 0 ou 120 dias. Utilizando a plataforma de sequenciamento Ion Torrent, foi possível observar uma redução significativa (P < 0,05) no número de OTUs ao se comparar silagens GSR aos 0 dias em relação aos 120 dias em ambos os híbridos e locais de cultivo do milho. As PCoAs evidenciaram variação na estrutura da comunidade bacteriana em função do período de estocagem do grão com separação nítida das comunidades provenientes de amostras recém colhidas daquelas provenientes de silagens estocadas por 120 dias. Também foi revelado que Proteobacteria (41,8 %), Firmicutes (41,6 %) e Actinobacteria (13,2 %) foram os filos mais abundantes nas silagens, tendo sido evidenciado a ocorrência de sucessão de um microbioma dominado por Proteobacteria e Actinobacteria (aos 0 dias) para um microbioma dominado por Firmicutes, representado principalmente pela ordem Lactobacillales nas silagens terminais (120 dias). Observamos o mesmo efeito ao considerar a amostra local, híbrido e maturidade fisiológica. Os fatores que apresentaram maior influência na distribuição da comunidade bacteriana foram o período de estocagem (36,16 %) e o estágio de maturação do milho (13,3 %). A ordem Lactobacillales foi diferencialmente abundante no milho estocado por 120 dias (70 % da variação) e Enterobacteriales (45 % da variação) aos 0 dias. Em relação ao estágio de maturação do grão observamos variação significativa na abundância relativa de Enterobacteriales em GU (25 % da variação) e Actinomycetales em GSR (21 % da variação). Houve correlação (P = 0,002) do pH com a estrutura da comunidade das silagens, sendo que as maiores variações de pH se deram comparando as amostras no tempo 0 - 120 dias de estocagem. A ordem Lactobacillales foi negativamente correlacionada com o pH (r = - 0,85), enquanto que Enterobacteriales (r = 0,58) e Actinomycetales (r = 0,46) foram positivamente correlacionadas com o pH. A reconstituição do grão de milho não exerceu efeito negativo sobre a população bacteriana das silagens terminais, destacando a viabilidade desta técnica. Até o momento, não há trabalhos publicados apresentando a estrutura e composição da comunidade bacteriana de silagens de GSR por meio de sequenciamento massivo do gene 16S rRNA, confirmando a importância e o ineditismo deste trabalho. / Understanding microbial ecology during ensiling is critical to neutralize critical points related to optimal silage making by preventing the growth of opportunistic bacteria that may compromise the safety of the animal food chain and the yield of silage fodder. Ensiling GSR makes it possible to buy strategically at times of low grain prices. The objective of this study was to evaluate the effect of reconstitution of corn grain on the dynamics of the bacterial community aiming high moisture corn silage processing by means of a massive sequencing of the 16S rRNA gene. Harvest was performed in two different locations. The plants used in the study included two contrasting hybrids, dent (AG 1051) and another flint (IAC 8390) hybrids, ensiled in two ways: wet grain (GU), ensiled with the original moisture or rehydrated dry grains (GSR) ensiled with 30 % of moisture, both ensiled for 0 or 120 days. Using the Ion Torrent sequencing platform, a significant reduction (P <0.05) in the number of OTUs was observed when comparing GSR silages at day 0 versus 120 days in both hybrids and maize growing sites. The PCoAs evidenced variation in the structure of the bacterial community as a function of the storage period of the grain with clear separation of the communities coming from freshly harvested samples from silage stored for 120 days. It was also revealed that Proteobacteria (41,8 %), Firmicutes (41,6 %) and Actinobacteria (13,2 %) were the most abundant phyla in the silages, evidencing the occurrence of succession of a microbiome dominated by Proteobacteria and Actinobacteria (at 0 day) for a microbiome dominated by Firmicutes, represented mainly by the order Lactobacillales in the terminal silages (120 days). We observed the same effect when considering the local sample, hybrid and physiological maturity. The factors that had the greatest influence on the distribution of the bacterial community were the storage period (36,16 %) and the stage of maturity of the plant (13,3 %). The Lactobacillales order was differentially abundant in the corn stored for 120 days (70% of the variation) and Enterobacteriales (45 % of the variation) at day 0. In relation to the stage of maturity we observed a significant variation in the relative abundance of Enterobacteriales in GU (25 % of the variation) and Actinomycetales in GSR (21 % of the variation). There was a correlation (P = 0.002) of the pH with the community structure of the silages, and the highest pH variations were obtained by comparing the samples in the 0 - 120 days of storage. The order Lactobacillales was negatively correlated with pH (r = -0.85), while Enterobacteriales (r = 0.58) and Actinomycetales (r = 0.46) were positively correlated with pH. The reconstitution of the corn grain did not have a negative effect on the bacterial population of the terminal silages, highlighting the viability of this technique. To date, there are no published papers presenting the structure and composition of the bacterial community of GSR silages by means of massive sequencing of the 16S rRNA gene, confirming the importance and novelty of this work.