Wildfires destroy large tracts of veld and forest land every year in South Africa. These fires can be devastating, resulting in loss of human lives, the destruction of property and the loss of income, for example the forest fire in the Sabie district in Mpumalanga in 2007 which destroyed about 7% of South Africa’s forested areas. There are frequently legal disputes with respect to the origin of wildfires, the extent of the fire and the land cover destroyed by the fires. The forensic capabilities of remote sensing in detecting and analysing post-wildfire characteristics have become an important contribution towards solving such legal disputes and in understanding wildfire characteristics. These post fire products can be used as evidence in court cases. Most of the time those court cases came up a few years after the fire event. By then, little or no evidence can be found on the terrain where the fire was. Remote sensing archives provide a reliable source of data that can be used to analyse these events after these long intervals. The objective of this project is to highlight the methods used to generate these post-wildfire analysis products. / Environmental Sciences / M. Sc. (Environmental Science)

Wildfires destroy large tracts of veld and forest land every year in South Africa. These fires can be devastating, resulting in loss of human lives, the destruction of property and the loss of income, for example the forest fire in the Sabie district in Mpumalanga in 2007 which destroyed about 7% of South Africa’s forested areas. There are frequently legal disputes with respect to the origin of wildfires, the extent of the fire and the land cover destroyed by the fires. The forensic capabilities of remote sensing in detecting and analysing post-wildfire characteristics have become an important contribution towards solving such legal disputes and in understanding wildfire characteristics. These post fire products can be used as evidence in court cases. Most of the time those court cases came up a few years after the fire event. By then, little or no evidence can be found on the terrain where the fire was. Remote sensing archives provide a reliable source of data that can be used to analyse these events after these long intervals. The objective of this project is to highlight the methods used to generate these post-wildfire analysis products. / Environmental Sciences / M. Sc. (Environmental Science)

<p>The development of low-cost, and reliable platforms for on-site detection of pathogenic agents, and toxic environmental traces is still a critical need for real-time monitoring of potential environmental pollution and imminent outbreaks. The biosensors market is projected to attain 31.5 billion by 2024. In this landscape, colorimetric and electrochemical devices continue to have significant relevance, with paper-based platforms leading the point-of-care (POC) segment for pathogen detection and environmental monitoring.</p> <p>Despite the true potential of biosensors in general, they have witnessed a slow rate in commercialization, mainly due to cost restrictions, and concerns related to their reliability and repeatability once scaled-up. This research evaluates the implementation of printing techniques as a strong approach for the fabrication of paper-based and flexible electrochemical biosensors. The results obtained demonstrated the ability to control and predict the variables affecting the sensing performance, achieving high precision of the printing parameters, and allowing optimization, and iterations since very early stages of prototype development.</p> <p>Besides the novel fabrication approach, this work introduces the use of truncated aptameric DNA sequences for whole cell detection of E. coli O157:H7 and heavy metals (Hg2+ and As3+), providing evidence of high stability and robustness under harsh conditions. Results obtained demonstrate their equal or even superior performance when compared to antibodies.</p> <p>We established the use of aptamer-functionalized multilayered label particles (PEI-grafted gold decorated polystyrene) with high stability as label particles. These particles address the well known drawback of non-selective aggregation typical of traditional naked Gold nanoparticles. The outstanding stability of these multilayered labels was demonstrated when used in an enhanced version of the lateral flow assay for detection of E. coli O157:H7 (state of the art for paper-based colorimetric detection of whole cell bacteria), and in a multiplexed paper-based microfluidic device for dual detection of Mercury and Arsenic. This work sets the foundation of the development of a next generation of health care and environmental monitoring devices that are portable, sensitive, quantitative, and can reliably detect multiple targets with one single test.</p>

<p>The last two decades have shown a boom in the field of wearable sensing technology. Particularly in the consumer industry, growing trends towards personalized health have pushed new devices to report many vital signs, with a demand for high accuracy and reliability. The most common technique used to gather these vitals is photoplethysmography or PPG. PPG devices are ideal for wearable applications as they are simple, power-efficient, and can be implemented on almost any area of the body. Traditionally PPGs were utilized for capturing just heart rate, however, recent advancements in hardware and digital processing have led to other metrics including respiratory rate (RR) and peripheral oxygen saturation (SpO2), to be reported as well. Our research investigates the potential for wearable devices to be used for outpatient apnea monitoring, and particularly the ability to detect opioid misuse resulting in respiratory depression. Ultimately, the long-term goal of this work is to develop a wearable device that can be used in the rehabilitation process to ensure both accountability and safety of the wearer. This document details contributions towards this goal through the design, development, and evaluation of a device called “Kick Ring”. Primarily, we investigate the ability of Kick Ring to record heartrate (HR), RR, and SpO2. Moreover, we show that the device can calculate RR in real time and can provide an immediate indication of abnormal events such as respiratory depression. Finally, we explore a novel method for reporting apnea events through the use of several PPG characteristics. Kick Ring reliably gathers respiratory metrics and offers a combination of features that does not exist in the current wearables space. These advancements will help to move the field forward, and eventually aid in early detection of life-threatening events.</p>