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京都大学 / 新制・課程博士 / 博士(工学) / 甲第24107号 / 工博第5029号 / 新制||工||1785(附属図書館) / 京都大学大学院工学研究科物質エネルギー化学専攻 / (主査)教授 安部 武志, 教授 作花 哲夫, 教授 阿部 竜 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM

Deep-marine sandstones containing significant (> 10%) detrital mud (silt and clay) matrix have become increasingly recognized, but mostly in drill core or poorly exposed outcrops where details of their vertical and lateral variability are poorly captured. Exceptional vertical and along-strike exposures of matrix-rich and associated matrix-poor deposits in deep-marine strata of the passive margin Neoproterozoic Windermere Supergroup and foreland basin Ordovician Cloridorme Formation, provide an unparalleled opportunity to document such characteristics. In both study areas, strata form a 100s m long depositional continuum that at its upflow end consists of thick-bedded matrix-poor sandstone (<20% matrix) that transforms progressively downflow to medium- to thick-bedded muddy sandstone (20 – 50% matrix) to medium-bedded bipartite facies with a basal sandy (30 – 60% matrix) part overlain sharply by a muddier part (40 – 80% matrix), and then to thin-bedded sandy mudstone (50 – 90% matrix). This depositional continuum is then overlain everywhere by a thin- to very thin-bedded traction-structured sandstone and/or silty mudstone cap. This consistent lithofacies change is interpreted to reflect particle settling in a rapidly but systematically evolving, negligibly-sheared sand-mud suspension developed along the margins (Windermere) and downflow terminus (Cloridorme) of a high-energy, mud-enriched avulsion jet. Stratigraphically upward, beds of similar lithofacies type succeed one another vertically and transform to the next facies in the depositional continuum at about the same along-strike position, forming stratal units 2–9 beds thick whose grain-size distribution gradually decreases upward. This spatial and temporal regularity is interpreted to be caused by multiple surges of a single, progressively waning turbidity current, with sufficient lag between successive surges for the deposition of a traction-structured sandstone overlain by mudstone cap. Furthermore, the systematic backstepping or side-stepping recognized at the stratal unit scale in both the Windermere and Cloridorme is interpreted to be driven by a combination of knickpoint migration and local topographic steering of the flows, which continued until the supply of mud from local seafloor erosion became exhausted, the main channel avulsed elsewhere, or a new stratal element developed.

Los métodos de Ingeniería Web dirigidos por modelos han mejorado tanto la calidad como la eficiencia a la hora de desarrollar aplicaciones Web. Estos métodos utilizan modelos conceptuales para capturar de forma abstracta una representación detallada de la aplicación Web a desarrollar. La ventaja más destacada de esta aproximación es que a partir de estos modelos, ampliamente validados en entornos industriales, es factible la generación sistemática del código que implementa la aplicación Web. Las aplicaciones Web 2.0 destacan, fundamentalmente, en la alta implicación de los usuarios a la hora de crear sus contenidos, ya sean en forma de opiniones, fotos, definiciones o videos. Este hecho enfatiza el carácter colaborativo de las aplicaciones Web 2.0 y otorga al usuario un rol primordial en la aplicación. Para lograr la colaboración del usuario, es un requisito imprescindible que la aplicación proporcione una interacción precisa e intuitiva. Este objetivo se ha conseguido en las aplicaciones Web 2.0 mediante: 1) una interfaz de usuario tecnológicamente muy avanzada y con un elevado grado de usabilidad; y 2) la reutilización de un conjunto de buenas prácticas, ampliamente aplicadas en el dominio de la Web 2.0, que proporcionan al usuario una forma sencilla y conocida de interactuar con la aplicación. Con el objetivo de desarrollar aplicaciones Web 2.0 de calidad, ambos aspectos tienen que estar presentes en los métodos de Ingeniería Web. Esta tesis doctoral presenta el método de Ingeniería Web dirigido por modelos OOWS 2.0 como una evolución incremental y necesaria del método OOWS. El objetivo esencial de este nuevo método es el de proporcionar la expresividad conceptual requerida para el desarrollo de aplicaciones Web 2.0. En concreto, las contribuciones se centran en soportar los aspectos avanzados de la interacción con el usuario final, tan relevantes en este tipo de aplicaciones. Para lograr esta meta, la tesis doctoral introduce una serie de modelos conceptuales que capturan, sin ambigüedades, las nuevas necesidades de interacción demandadas por las aplicaciones Web 2.0. / Valverde Giromé, F. (2010). OOWS 2.0: UN MÉTODO DE INGENIERÍA WEB DIRIGIDO POR MODELOS PARA LA PRODUCCIÓN DE APLICACIONES WEB 2.0 [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/8977

Expert bodies recommend that populations adopt plant-rich dietary patterns and consume less red and processed meats (RPM) as a high-impact climate action. This PhD dissertation describes three studies that examined how diverse food and health systems actors may encourage sustainable diet transitions for Americans to support human and planetary health. Study one examined U.S. adults' perceptions, beliefs, and behaviors toward plant-rich dietary patterns. The International Food Information Council's Food and Health Surveys (2012–2022) were analyzed using crosstabulation and chi-square analyses. Consumers' recent RPM intake trends were mixed. Despite interest in sustainable products and principles, few U.S. adults followed plant-rich dietary patterns or purchasing practices. Leadership and coordinated action are needed to incentivize Americans to adopt plant-rich dietary behaviors. Study two conducted a systematic scoping review of media campaigns that promoted plant-rich dietary patterns and traditional and novel plant-based proteins, and that encouraged or discouraged RPM products to Americans (1917-2023). Of 84 media campaigns identified, corporate marketing (58.6%) campaigns were most prevalent compared to public information (13.8%), corporate sustainability (12.6%), countermarketing (5.7%), social marketing (4.6%), and public policy (4.6%). Civil society campaigns promoted plant-rich dietary patterns, but only one campaign was evaluated. U.S. government, academia, businesses, and civil society should commit adequate resources and evaluate media campaigns to support a sustainable diet transition for Americans that prioritizes traditional and novel plant-based proteins. Study three explored U.S. Food is Medicine (FIM) experts' views on how plant-rich dietary patterns and other sustainable diet practices could be incorporated into FIM interventions. Twenty semi-structured interviews were conducted among U.S. food and health systems actors and analyzed using inductive and deductive thematic analysis. Results indicated that many FIM actions support human and planetary health, but the co-benefits are not often discussed. The FIM movement is a unique opportunity to promote food and health systems changes that support human and planetary health, but key challenges require coordinated action across sectors. The three studies in this PhD dissertation collectively addressed knowledge gaps, used novel conceptual frameworks, and offered recommendations to inform U.S. food and nutrition policies, programs, and research to encourage sustainable diet transitions for American adults. / Doctor of Philosophy / Expert bodies recommend that populations adopt plant-rich dietary patterns, or ways of eating that emphasize intake of plant-based foods (i.e., whole grains, fruits, vegetables, legumes, nuts, and seeds) and de-emphasize or avoid intake of animal-sourced foods, especially red and processed meats (RPM). Plant-rich dietary patterns can reduce diet-related disease risks and minimize the food system's negative environmental contributions. This PhD dissertation describes three studies that examined how diverse food and health systems actors may encourage Americans to transition towards plant-rich dietary patterns and reduce RPM intake to support human and planetary health. Study one analyzed 11 years of survey data (2012–2022) to examine U.S. adults' perceptions, beliefs, and behaviors toward plant-rich dietary patterns. Consumers' recent RPM intake trends were mixed, with some reporting increased consumption and others reporting reduced RPM intake. Few adults reported adherence to plant-rich dietary patterns. Leadership and coordinated action are needed to incentivize Americans to adopt plant-rich dietary behaviors. Study two reviewed published literature to identify the landscape of media campaigns that promoted plant-rich dietary patterns and plant-based proteins, and that encouraged or discouraged RPM products to Americans (1917-2023). Of 84 media campaigns identified, most (58.6%) campaigns promoted specific food products. Only one campaign was evaluated. U.S. government, academia, businesses, and civil society should commit adequate resources and evaluate media campaigns that promote plant-rich dietary patterns and plant-based food products. Study three explored U.S. Food is Medicine (FIM) experts' views on how plant-rich dietary patterns and other sustainable diet practices could be incorporated into FIM interventions, which aim to connect food to the health system. Twenty interviews were conducted among U.S. food and health systems actors and analyzed for key themes. Results indicated that many FIM actions support human health and the environment, but the co-benefits are not often discussed. The FIM movement is a unique opportunity to promote food and health systems changes that support human health and environmental sustainability, but key challenges require coordinated action across sectors. These three studies help inform how food and health systems actors can support Americans to adopt dietary practices that promote human health and the environment.

Soybean is a dominant oilseed cultivated worldwide for its use in multiple sectors such as food and feed industries, animal husbandry, cosmetics and pharmaceutical sectors, and more recently, in production of biodiesel. Increasing demand of soybean, changing environmental conditions, and evolution of pathogens pose challenges to soybean production in limited acreage. Genetic research is the key to ensure the continued growth in soybean production, with enhanced yield and quality, while reducing the losses due to diseases and pests. This research is focused on the understanding of transcriptional regulation of two economically important agronomic traits of soybean: low seed phytic acid and resistance to Soybean mosaic virus (SMV), using the 'transcriptomics' and 'genomics' approaches. The low phytic acid (lpa) soybean is more desirable than conventional soybean, as phytic acid is an anti-nutritional component of seed and is associated with phosphorus pollution. Despite the eco-friendly nature of the lpa soybean, it shows poor emergence, which reduces soybean yield. This research is mainly focused on addressing the impact of lpa-causing mutations on seed development, which is suspected to cause low emergence in lpa soybeans. The differences in transcriptome profiles of developing seeds in lpa and normal phytic acid soybean are revealed and the biological pathways that may potentially be involved in regulation of seed development are suggested. The second research project is focused on Rsv3-type resistance, which is effective against most virulent strains of Soybean mosaic virus. The Rsv3 locus, which maps on to soybean chromosome 14, contains 10 genes including a cluster of coiled coil-nucleotide binding-leucine rich repeat (CC-NB-LRR) protein-encoding genes. This dissertation employed a comparative sequencing approach to narrow down the list of Rsv3 gene candidates to the most promising CC-NB-LRR gene. The evidence provided in this study clearly indicates a single CC-NB-LRR gene as the most promising candidate to deliver Rsv3-type resistance. / Ph. D.

Soybean mosaic virus (SMV) is the most common member of the viral genus Potyvirus to infect soybeans (Glycine max [L.] Merr.) worldwide. SMV has been traditionally controlled by the deployment of single dominant, strain specific resistance genes, referred to as Rsv genes. Rsv1 is the most widely used form of SMV resistance with nine different alleles conferring resistance only to the lower numbered less virulent strains, G1 to G3. Rsv3 gives resistance to higher numbered more virulent strains G5 to G7. Soybean lines containing Rsv4, are resistant to all seven currently recognized North American SMV strains. In this study, the recently released soybean whole genome sequence was used to design molecular markers for fine mapping Rsv3 to a ~150 kb genomic region containing four coiled-coil nucleotide-binding leucine-rich repeat proteins. In a related study a large population segregating at the Rsv3 locus was screened for resistance to facilitate future characterization of this region. The markers identified in this study will allow for more accurate marker-assisted selection of Rsv3. / Master of Science

Glycogen phosphorylase 2 (gp-2) is a key enzyme during the development of Dictyostelium discoideum. The gp-2 enzyme breaks down glycogen into glucose monomers that are subsequently used to synthesize the terminal end products of cellular differentiation. This gene is an ideal candidate for studying the process of selective gene expression because its product figures so prominently in the development of this organism, implying a dependable control mechanism responsible for its developmentally regulated expression. I present in this thesis the identification of several putative cis-acting elements of gp-2 as revealed through footprint analysis. Due to the extreme AT-bias characteristic of Dictyostelium promoters, footprinting conditions required intensive optimization with respect to template, nonspecific competitor, source of protein extract and DNase I digestion. Using an endlabeled fragment containing seven repeated sequences (3 TA boxes [TAATTATA], 2 TAG boxes [TAAAAATGGT] and 2 C boxes [ACCCACT]), purified replication protein A and several developmental nuclear extracts were tested for DNA binding activity. Small footprints were observed on the TAG and C boxes of the promoter for both protein sources. However, using a more sensitive footprinting strategy involving multiple rounds of primer extension, larger footprints spanning the same promoter regions were detected. In both cases, the appearance of the footprints coincided with the documented transcriptional activity of the gene. It can be concluded from the data obtained that the TAG and C boxes are very likely cis-acting elements involved in the regulation of gp-2 expression. / Master of Science

京都大学 / 新制・課程博士 / 博士(人間・環境学) / 甲第25385号 / 人博第1127号 / 新制||人||262(附属図書館) / 京都大学大学院人間・環境学研究科相関環境学専攻 / (主査)教授 内本 喜晴, 教授 田部 勢津久, 教授 藤原 直樹, 教授 雨澤 浩史 / 学位規則第4条第1項該当 / Doctor of Human and Environmental Studies / Kyoto University / DFAM

This thesis reports case studies of students working with context rich problems (CRP) and mini projects (MP) in physics in an upper secondary school class and in a physics teacher education class at university. The students report a big shift from physics in secondary school as fun and easy, to physics in upper secondary school as boring, difficult and with lack of time for reflections and physics talking, but they also found physics as interesting in itself. In order to study how group discussions in physics influence the students learning and to study the phenomena of students’ ownership of learning (SOL) we introduced CRP and MP. We video recorded five groups with 14 teacher students at university in the end of 2002, and five group with 15 students at upper secondary school during the beginning of their second physics course in the spring term in 2003. MP and CRP in physics were used as instructional settings in order to give students possibility to strengthen their holistic understanding and their possibilities to ownership. When students get the opportunity to manage their own learning and studying by open-ended tasks in physics, without the teacher determining all details of the performance, this gives more ownership of learning. The advantage of MPs and CRPs from the student’s point of view is more freedom to act, think and discuss and from the teacher’s view, to get insights of the students’ ability and how they really think in physics. The ownership is found to be crucial for motivation and development of competence. Students’ ownership of learning (SOL) is the students’ influence/impact to affect tasks and the learning environment in such a way that the students have a real opportunity to achieve learning of physics. Students’ ownership of learning (SOL) is found at two levels: Group level: At the start of a task the SOL is determined by the design of the task. The choice of task, the performance (when, how, where), the level of result and presentatio n and report have to be determined by the students themselves. Individual level: A person’s experiences and anomalies of understanding have created unique questions that can create certain aspects of the task that drive this person to be very active and highly motivated. This gives the person a high individual ownership. We developed hypotheses concerning the relation between ownership, motivation and competence and we see some evidence in the cases reported in this thesis. The importance of exploratory talks to enhance learning, and to see aspects of communication as part of the motivation are discussed in the model of ownership, motivation and competence that is proposed.