Para alimentar a população mundial crescente é necessário um aumento sustentável na produtividade agrícola. Nesse sentido, Rizobactérias Promotoras de Crescimento de Plantas (RPCPs) têm sido continuamente buscadas para formulações inoculantes por sua capacidade de incremento na produção vegetal aliado ao seu potencial de redução e/ou substituição do uso de fertilizantes minerais, insumos que causam grandes impactos ambientais, na saúde humana e econômicos. A RPCP Bacillus sp. RZ2MS9, um representante da biodiversidade amazônica brasileira, é uma forte candidata a bionoculante por seu efeito benéfico, previamente descrito, em uma ampla gama de culturas, incluindo milho e soja. Essas duas culturas representam mais de 80% da área cultivada com grãos no Brasil, de forma que incrementos relativamente modestos de crescimento e produtividade poderiam gerar ganhos significativos. Membros do gênero Bacillus apresentam vantagem em formulações inoculantes, principalmente devido a sua capacidade de formação de esporos resistentes ao calor e dissecação. Seus modos de ação são diversos, tornando o entendimento da sua interação com plantas bastante desafiador. Bacillus sp. RZ2MS9 apresentou, dentre os mecanismos envolvidos na promoção de crescimento vegetal, a produção de Ácido Indol Acético (AIA) e sideróforos, solubilização de fosfato e fixação biológica de nitrogênio, in vitro. No presente trabalho, foi buscado um entendimento detalhado dos mecanismos de ação dessa rizobactéria, explorando desde seu genoma até seu desempenho em condições de campo. O draft genômico (genoma parcial) bacteriano foi obtido utilizando a tecnologia de sequenciamento Illumina, o qual possibilitou a detecção de genes envolvidos nos mecanismos potencialmente relacionados ao efeito benéfico dessa bactéria, que vão desde sua formação de esporos, atração por exsudatos radiculares, motilidade e competição na rizosfera até mecanismos de solubilização de fosfato, produção de sideróforos, entre outros. As informações obtidas permitem uma exploração genética desses mecanismos, fornecendo uma oportunidade de maximizar essa interação e, futuramente, favorecer os benefícios em campo. Adicionalmente, foi demonstrado o potencial de quimiotaxia (atração) de RZ2MS9 em direção a raízes de milho. Um estudo filogenético dessa RPCP, utilizando um método de tipagem com o gene pycA (piruvato carboxilase), mostrou que o Bacillus sp. RZ2MS9 apresentou-se distante do clado altamente monomórfico de B. anthracis, patógeno humano, e se afiliou a um grupo composto por linhagens de B. thuringiensis (Bt) comercializadas como produtos biopesticidas há mais de 60 anos, o que sugere a potencial possibilidade de seu uso seguro no campo. Sabe-se que a maioria, se não todas, atividades fisiológicas das plantas é regulada por fitormônios como a auxina AIA, os quais podem ser sintetizados também por RPCPs. Com mais detalhamento, os genes envolvidos nas vias biossintéticas desse fitormônio foram detectados no draft genômico de RZ2MS9, indicando que sua produção ocorre através da via IPA (Indol-3-Piruvato). Além disso, plantas de tomate anão Micro-Tom (MT) e seu mutante Δdgt, defectivo na sensibilidade a auxinas, foram utilizadas para caracterizar especificamente o efeito do AIA produzido por Bacillus sp. RZ2MS9 na promoção de crescimento vegetal. A aplicação de RZ2MS9 causou inibição no crescimento de raízes primárias, aumento no comprimento de raízes laterais e na área superficial total de raízes de plantas MT, efeitos característicos daqueles proporcionados por auxinas. Esse incremento radicular refletiu, ainda, em aumento da biomassa da parte aérea de plantas MT. Os mesmos efeitos não foram observados em plantas Δdgt, insensíveis a auxinas, indicando que a elicitação de promoção de crescimento em MT por RZ2MS9 ocorre por meio desses fitormônios. Finalmente, foi demonstrado o efeito sobre o desenvolvimento e produtividade de milho e soja da aplicação de Bacillus sp. RZ2MS9 em condições de campo, sendo comparado com o desempenho de bioinoculantes comerciais. No milho, o efeito da inoculação bacteriana foi, ainda, associado à adubação nitrogenada para verificar a possibilidade de redução desses insumos. Bacillus sp. RZ2MS9 apresentou efeitos significativos sobre o desenvolvimento tanto da soja (comparáveis aos efeitos de rizóbios) quanto do milho, os quais, porém, não refletiram em aumento significativo de produtividade em ambas as culturas. No entanto, o potencial dessa rizobactéria é bastante claro pois, com um custo de produção inferior a R$1,00 por hectare, sua inoculação causou incremento de 16 sacas de milho por hectare com redução de 30% na adubação nitrogenada, assim como um incremento de 11 sacas de soja por hectare, ambos comparados ao controle não inoculado. Os resultados apresentados no presente trabalho vão, portanto, de encontro à grande expectativa na obtenção de linhagens microbianas promissoras visando sistemas agrícolas mais sustentáveis. / To feed the growing global population, a sustainable increase of agricultural production and crop yield is required. In this sense, Plant Growth Promoting Rhizobacteria (PGPR) have been continuously sought to inoculant formulation due to their capacity to increase plant yield along with their potential to reduce and/or replace the use of mineral fertilizers, inputs that cause serious impacts on environment, human health and economy. The PGPR Bacillus sp. RZ2MS9, a representative of the Brazilian Amazonian biodiversity, is a great candidate to bioinoculant because of its beneficial effect on a broad range of crops, including maize and soybean. These two crops represent more than 80% of the area planted with grains in Brazil, so relatively modest growth and yield increases could generate significant gains. Bacillus spp. have advantage in inoculant formulations, mainly due to their ability to form heat- and dissecation-resistant spores. Their modes of action are diverse, making the understanding of its interaction with plants quite challenging. Bacillus sp. RZ2MS9 displays, between the mechanisms involved in plant growth, Indole Acetic Acid (IAA) and siderophore production, phosphate solubilization and biological nitrogen fixation, in vitro. In the present work, we seek a detailed understanding of this rhizobacterium mechanisms of action, exploring from its genome to its performance in field conditions. The bacterial draft genome was obtained using Illumina sequencing technology, making possible the detection of genes involved in mechanisms potentially related to the beneficial effect of this bacterium, and range from its spore formation, attraction by root exudates, motility and competition in the rhizosphere to mechanisms of phosphate solubilization, siderophore production, among others. The information obtained allow a genetic exploration of these mechanisms, providing an opportunity to maximize this interaction and, in the future, favor benefits in field. Additionally, it was demonstrated the chemotaxis (attraction) potential of RZ2MS9 towards maize roots. A phylogenetic study of this PGPR, using a typing method with the pycA (pyruvate carboxylase) gene, showed that Bacillus sp. RZ2MS9 was distant from the highly monomorphic clade of B. anthracis, a human pathogen, and affiliated with B. thuringiensis (Bt) strains marketed as biopesticides for more than 60 years, suggesting the potential possibility of its safe use in the field. It is known that most, if not all, physiological activities of plants are regulated by phytormones such as the auxin IAA, which can also be synthesized by PGPRs. With more detail, genes involved in biosynthetic pathways of this phytormone were detected in the RZ2MS9 draft genome, indicating that its production occurs via the IPA (indole-3-pyruvate) pathway. In addition, plants of the dwarf tomato Micro-Tom (MT) and its mutant Δdgt, impaired in auxin sensibility, were used to specifically characterize the effects of IAA produced by Bacillus sp. RZ2MS9 in the plant growth promotion. The inoculation of RZ2MS9 caused inhibition in the primary roots growth, increase in lateral roots length and in roots total surface area of MT plants, characteristic effects of those provided by auxins. This root growth also reflected in an increase of MT plants shoot biomass. The same effects were not observed in Δdgt plants, insensitive to auxins, suggesting that the elicitation of growth promotion in MT by RZ2MS9 occurs through these phytormones. Finally, we demonstrated the effect of inoculation with Bacillus sp. RZ2MS9 on maize and soybean development and productivity under field conditions, being compared with the performance of commercial bioinoculants. In maize, the effect of bacterial inoculation was also associated with nitrogen fertilization to verify the possibility of reducing these inputs. Bacillus sp. RZ2MS9 showed significant effects on the development of both soybean (comparable to the effects of rhizobia) and maize, which, however, did not reflect a significant increase in productivity in both crops. However, the potential of this rhizobacterium is very clear because, with a cost of production of less than R$1.00 per hectare, its inoculation caused an increase of 16 sacks of maize per hectare with a 30% reduction in nitrogen fertilization, as well as an increase of 11 sacks of soybean per hectare, both compared to uninoculated control. The results presented in this study meet the great expectation of obtaining promising microbial strains aiming at more sustainable agricultural systems.

Over the past decade '"Sustainable Development" (SD) has emerged as the latest development catchphrase. A wide range of nongovernmental as well as governmental organizations have embraced it as the new paradigm of development. A review of the literature that has sprung up around the concept of SD indicates, however, a lack of consistency in its interpretation. More important, while the all-encompassing nature of the concept gives it political strength, its current formulation by the mainstream of SD thinking contains significant weaknesses. These include an incomplete perception of the problems of poverty and environmental degradation, and confusion about the role of economic growth and about the concept of sustainability. The purpose of this study was to identify common elements in a political economy of the environment, relating environmental change to the dynamics of ideology and policy, and at different levels of political complexity. The intention was to provide a structural analysis of the environment in which the development process illuminates environmental change at both a philosophical and material level. The problem in achieving SD was related to the overriding structures of the international economic system, which have arisen out of the exploitation of environmental resources, and which frequently operate as constraints on the achievement of long-term sustainable practices. Insufficient accounting of ecological aspects of economic growth and development has resulted from intellectual traditions, where solutions are formulated, point in different directions. Conclusions are drawn that SD involves trade-offs between biological, economic and social systems and is found in the interactive zone between these systems. There are a number of international factors that may be necessary, but insufficient, conditions for SD on a national level, including peace, debt reduction, and more propitious terms of trade. There was seen dilemmas relating to SD, including the role of growth as the unquestioned objective of economic policy.

Traditional agricultural practices often result in gaseous losses of nitrous oxide (N2O), ammonia (NH3), and carbon dioxide (CO2), representing a net loss of nutrients from agricultural soils, which negatively impacts crop yield and requires farmers to increase nutrient inputs. By adopting best management practices (BMPs; i.e., no-tillage, cover crops, sub-surface manure application, and proper manure application timing), there is great potential to reduce these losses. Because N2O and CO2 are also greenhouse gases (GHGs), climate change mitigation via BMP adoption and emissions reductions would be an important co-benefit. However, adopting a no-tillage and cover cropping system has had setbacks within the Northeast, primarily due to concerns regarding manure nitrogen (N) losses in no-tillage systems as well as uncertainty surrounding the benefits of cover crops. This thesis used two field-trials located in Alburgh, Vermont to assess differences in (i) GHG emissions from agricultural soils, (ii) nitrate and ammonium retention, (iii) corn yield and protein content, and (iv) N uptake and retention via cover crop scavenging under a combination of different BMPs. Chapter 1 evaluates the effects of different reduced-tillage practices and manure application methods (i.e., vertical-tillage, no-tillage, manure injection, and broadcast manure application) on reducing N2O and CO2 emissions, retaining inorganic N, and improving crop yields. Greenhouse gas measurements were collected every other week for the growing season of 2015-2017 via static chamber method using a photoacoustic gas analyzer. Results from this study showed that tillage regimes and manure application method did not interact to affect any of the three research objectives, although differences between individual BMPs were observed. Notably, vertical tillage enhanced CO2 emissions relative to no-tillage, demonstrating the role of soil disturbance and aeration on aerobic microbial C transformations. Manure injection was found to significantly enhance both N2O and CO2 emission relative to broadcast application, likely due to the formation of anerobic micro-zones created from liquid manure injection. However, plots that received manure injection retained greater concentrations of soil nitrate, a vital nutrient for quality crop production, thereby highlighting a major tradeoff between gaseous N losses and N retention with manure injection. Chapter 2 evaluates the effects of tillage practices and timing of manure application to increase N retention with the use of cover crops in order to mitigate GHG emissions, enhance soil nitrate and ammonium retention, and improve cropping system N uptake. Treatments at this field trial consisted of a combination of the presence or absence of cover crops, no-tillage or conventional-tillage, and spring or fall manure application. Greenhouse gas emissions were measured every other week via static chamber method using a gas chromatograph for the growing season of 2018. Results from this study showed that the presence of cover crops enhanced both N2O and CO2 emissions relative to fallow land, irrespective of tillage regime and manure application season, likely as a result of greater N and carbon substrates entering the soil upon cover crop decomposition. Due to enhanced N2O emissions with cover crops, cover crops did not retain significantly greater inorganic N in the system upon termination.

No description available.

The focus of the thesis is a critique of the form and function of the federal institutions governing the development of Rural Australia. In undertaking this study two cycles of a systemic action research were followed, the first to explore the policy development environment and the second to validate and expand on findings of the first cycle of enquiry. The thesis follows the historical development of policy institutions and the deliberations of poicy actors that have lead to normative, strategic and program change within these institutions. These institutional changes have then been critiqued from theoretical perspective of governance for sustainable development. Conclusions from this critique indicate that that the pace of policy change is very slow and after 20 years from the Rural Policy green paper of 1974 the federal institutions have taken on board a rhetoric of sustainable rural development that encapsulates much of the principles espoused in the Green Paper.This includes principles that aim to empower rural communities to find local solutions to their natural resource management and local economic development problems. The downside is found in institutional conflict over resource dependencies and spheres of responsibility and an apparent lack of community economic development facilitation skills within the service organisations of rural institutions. / Master of Science (Hons)

Doctor of Philosophy / Precision Agriculture (PA) strives towards holistic production and environmental management. A fundamental research challenge is the continuous expansion of ideas about how PA can contribute to sustainable agriculture. Some associated pragmatic research challenges include quantification of spatio-temporal variation of crop yield; crop growth simulation modelling within a PA context and; evaluating long-term financial and environmental outcomes from site-specific crop management (SSCM). In Chapter 1 literature about managing whole farms with a mind towards sustainability was reviewed. Alternative agricultural systems and concepts including systems thinking, agro-ecology, mosaic farming and PA were investigated. With respect to environmental outcomes it was found that PA research is relatively immature. There is scope to thoroughly evaluate PA from a long-term, whole-farm environmental and financial perspective. Comparatively, the emphasis of PA research on managing spatial variability offers promising and innovative ways forward, particularly in terms of designing new farming systems. It was found that using crop growth simulation modelling in a PA context is potentially very useful. Modelling high-resolution spatial and temporal variability with current simulation models poses a number of immediate research issues. This research focused on three whole farms located in Australia that grow predominantly grains without irrigation. These study sites represent three important grain growing regions within Australia. These are northern NSW, north-east Victoria and South Australia. Note-worthy environmental and climatic differences between these regions such as rainfall timing, soil type and topographic features were outlined in Chapter 2. When considering adoption of SSCM, it is essential to understand the impact of temporal variation on the potential value of managing spatial variation. Quantifying spatiotemporal variation of crop yield serves this purpose; however, this is a conceptually and practically challenging undertaking. A small number of previous studies have found that the magnitude of temporal variation far exceeds that of spatial variation. Chapter 3 of this thesis dealt with existing and new approaches quantifying the relationship between spatial and temporal variability in crop yield. It was found that using pseudo cross variography to obtain spatial and temporal variation ‘equivalents’ is a promising approach to quantitatively comparing spatial and temporal variation. The results from this research indicate that more data in the temporal dimension is required to enable thorough analysis using this approach. This is particularly relevant when questioning the suitability of SSCM. Crop growth simulation modelling offers PA a number of benefits such as the ability to simulate a considerable volume of data in the temporal dimension. A dominant challenge recognised within the PA/modelling literature is the mismatch between the spatial resolution of point-based model output (and therefore input) and the spatial resolution of information demanded by PA. This culminates into questions about the conceptual model underpinning the simulation model and the practicality of using point-based models to simulate spatial variability. iii The ability of point-based models to simulate appropriate spatial and temporal variability of crop yield and the importance of soil available water capacity (AWC) for these simulations were investigated in Chapter 4. The results indicated that simulated spatial variation is low compared to some previously reported spatial variability of real yield data for some climate years. It was found that the structure of spatial yield variation was directly related to the structure of the AWC and interactions between AWC and climate. It is apparent that varying AWC spatially is a reasonable starting point for modelling spatial variation of crop yield. A trade-off between capturing adequate spatio-temporal variation of crop yield and the inclusion of realistically obtainable model inputs is identified. A number of practical solutions to model parameterisation for PA purposes are identified in the literature. A popular approach is to minimise the number of simulations required. Another approach that enables modelling at every desired point across a study area involves taking advantage of high-resolution yield information from a number of years to estimate site-specific soil properties with the inverse use of a crop growth simulation model. Inverse meta-modelling was undertaken in Chapter 5 to estimate AWC on 10- metre grids across each of the study farms. This proved to be an efficient approach to obtaining high-resolution AWC information at the spatial extent of whole farms. The AWC estimates proved useful for yield prediction using simple linear regression as opposed to application within a complex crop growth simulation model. The ability of point-based models to simulate spatial variation was re-visited in Chapter 6 with respect to the exclusion of lateral water movement. The addition of a topographic component into the simple point-based yield prediction models substantially improved yield predictions. The value of these additions was interpreted using coefficients of determination and comparing variograms for each of the yield prediction components. A result consistent with the preceding chapter is the importance of further validating the yield prediction models with further yield data when it becomes available. Finally, some whole-farm management scenarios using SSCM were synthesised in Chapter 7. A framework that enables evaluation of the long-term (50 years) farm outcomes soil carbon sequestration, nitrogen leaching and crop yield was established. The suitability of SSCM across whole-farms over the long term was investigated and it was found that the suitability of SSCM is confined to certain fields. This analysis also enabled identification of parts of the farms that are the least financially and environmentally viable. SSCM in conjunction with other PA management strategies is identified as a promising approach to long-term and whole-farm integrated management.

In the semi - arid Mediterranean - type environments of southern Australia, soil and water resources largely determine crop productivity and ultimately the sustainability of farming systems within the region. The development of sustainable farming systems is a constantly evolving process, of which cropping sequences ( rotations ) are an essential component. This thesis focused on two important soil resources, soil water and nitrogen, and studied the effects of different crop sequences on the dynamic of these resources within current farming systems practiced on the upper Eyre Peninsula of South Australia. The hypothesis tested was that : continuous cropping may alter N dynamics but will not necessarily alter water use efficiency in semi - arid Mediterranean - type environments. Continuous cropping altered N - dynamics ; increases in inorganic N were dependent on the inclusion of a legume in the cropping sequence. Associated with the increase in inorganic N supply was a decrease in WUE by the subsequent wheat crop. Overall, estimates of water use efficiency, a common index of the sustainability of farming systems, in this study concur with reported values for the semi - arid Murray - Mallee region of southern Australia and other semi - arid environments worldwide. Soil water balance and determination of WUE for a series of crop sequences in this thesis suggests that the adoption of continuous cropping may increase WUE and confer a yield benefit compared to crop sequences including a legume component in this environment. No differences in total water use ( ET ) at anthesis or maturity were measured for wheat regardless of the previous crop. Soil evaporation ( E [subscript s] ) was significantly affected by crop canopy development, measured as LAI from tillering until anthesis in 2002, however total seasonal E [subscript s] did not differ between crop sequences. Indeed in environments with infrequent rainfall, such as the upper Eyre Peninsula, soil evaporation may be water - limited rather than energy limited and the potential benefits from greater LAI and reduced E [subscript s] are less. Greater shoot dry matter production and LAI due to an enhanced inorganic N supply for wheat after legumes, and to a lesser degree wheat after canola, relative to continuous cereal crop sequences resulted in increases in WUE calculated at anthesis, as reported by others. Nonetheless the increase in WUE was not sustained due to limitations on available soil water capacity caused by soil physical and chemical constraints. Access to more soil water at depth ( > 0.8m ) through additional root growth was unavailable due to soil chemical limitations. More importantly, the amount of plant available water within the ' effective rooting depth ' ( 0 - 0.8m ) was significantly reduced when soil physical factors were accounted for using the integral water capacity ( IWC ) concept. The difference between the magnitude of the plant available water capacity and the integral water capacity was approximately 90mm within the ' effective rooting depth ' when measured at field capacity, suggesting that the ability of the soil to store water and buffer against periodic water deficit was severely limited. The IWC concept offers a method of evaluating the physical quality of soils and the limitations that these physical properties, viz. aeration, soil strength and hydraulic conductivity, impose on the water supply capacity of the soil. The inability of the soil to maintain a constant supply of water to satisfy maximal transpiration efficiency combined with large amounts of N resulted in ' haying off ', and reduced grain yields. A strong negative linear relationship was established between WUE of grain production by wheat and increasing soil NO [subscript 3] - N at sowing in 2000 and 2002, which conflicts with results from experiments in semi - arid Mediterranean climates in other regions of the world where applications of N increased water use efficiency of grain. Estimates of proportional dependence on N [subscript 2] fixation ( % N [subscript dfa] ) for annual medics and vetch from this study ( 43 - 80 % ) are comparable to others for environments in southern Australia ( < 450mm average annual rainfall ). Such estimates of fixation are considered low ( < 65 % ) to adequate ( 65 - 80 % ). Nevertheless, the amount of plant available N present at sowing for subsequent wheat crops, and the occurrence of ' haying off ', suggests that WUE is not N - limited per se, as implied by some reports, but constrained by the capacity of a soil to balance the co - limiting factors of water and nitrogen. / Thesis (Ph.D.)--School of Earth and Environmental Sciences, 2005.

<p>Botanical pesticides are agricultural pest management agents which are based on plant extracts. In modern times these have been used as alternatives to synthetic chemicals in organic pest management. The practice of using plant materials against field and storage pests however has a long history in many indigenous and traditional farming communities across the world. During February and March 2009 a field study was conducted in Babati district in Manyara region, Tanzania to investigate the local use of botanical pesticides. The results from the field study were subsequently analyzed and contextualized in Nicanor Perlas model The Seven Dimensions of Sustainable Agriculture which was used as a framework theory. The analysis indicates that there are a variety of thresholds for the use of botanical pesticides in sustainable agriculture in Babati district besides the more obvious practical aspects. The latter parts of the paper discuss the differences between reductionist and holistic, indigenous and scientific ways of achieving knowledge with regard to plant based pesticides. The study concludes that ecological pest management is a holistic method based on the synergy of a variety of farming practices. Indigenous knowledge which is holistic, site-specific and experience based has therefore much to offer modern endeavours to practice a more sustainable agriculture and pest management strategies which consider the welfare of both humanity and the environment.</p>

Maya Mopan farmers in southern Belize face socio-economic hardships, persisting environmental constraints, and an unfavorable political climate that has prevented land tenure stability on reservation lands. This thesis describes the agricultural practices of a group of Mopan farmers and examines farm-site diversification and its relationship to ecological knowledge, out-migration, agricultural markets, and indigenous political systems. I also examine how modernization, religious conversions, and the Mopan's introduction into a capitalist economy have led to a decline in cultural practices and the augmentation of non-traditional behaviors among the younger generations. Data were obtained during my fieldwork in the Cayo and Toledo Districts of southern Belize from June-November, 2002. I conducted informal interviews with farmers and NGOs, engaged in participant observation techniques, documented 17 diversified Mayan farms, and formulated a self-administered questionnaire that was given to 38 students in the San Jose Village School. Additional data was acquired through voluntary work in farmers' fields and from available anthropological and agricultural literature. The results of this study indicate that Mopan farmers have diversified their farming systems by adopting new crop varieties, developing more sustainable agricultural techniques, increasing the production of cash crops, and adjusting their traditional labor systems. These findings are significant because they demonstrate ways in which farming communities throughout the tropics can improve their environments and economies amidst the influences of modernization, unsustainable development, and discriminatory government policies. / Graduation date: 2005

Thesis (Ph. D.)--Ohio State University, 2006. / Title from first page of PDF file. Includes bibliographical references (p. 526-547).