Cette thèse presente des travaux et résultats d'expériences de fracturation dont l'origine est une génération interne de fluides induite par des réactions chimiques dans des échantillons de roches et des matériaux analogues. Les deux premiers articles portent sur une expérience avec des échantillons de schistes imagés par tomographie aux rayons X au cours du temps. Le premier article décrit la formation de fractures créées par l'augmentation de pression induite par la décomposition de matière organique lorsque les schistes sont chauffés. Le deuxième article porte sur la procédure expérimentale et l'analyse d'images, utilisées pour obtenir les résultats du premier article. Les deux autres articles concernent la fracturation d'une couche de gélatine contenant de la levure et du sucre, ce qui génère du CO2. Le troisième article décrit le mécanisme de formation d'un réseau de fractures au cours du drainage du CO2 contenu dans la gélatine. Le quatrième article concerne l'évolution du réseau de fractures au cours de ce drainage, ainsi que les mécanismes d'ouverture et fermeture des fractures. Le dernier article porte sur une étude par tomographie aux rayons X de la distribution de porosité d'échantillons endommagés d'andésite. Les méthodes développées dans ce projet peuvent s'appliquer à la déshydratation de sédiments, la formation de volcans de boue, l'exploration d'hydrates de méthane, la séquestration de CO2 et la fracturation de réservoirs non conventionnels. / This thesis presents the experimental work and the results on fracturing of rock samples and analogue materials due to internal fluid generation during chemical reaction. The first two papers concentrate on time-resolved 3D X-ray imaging experiment on organic-rich shale samples. Paper 1 describes fracture formation due to hydrocarbon generation in the shale induced by organic matter decomposition during heating. Paper 2 gives an overview of the experimental procedure and image analysis workflow, which were used to obtain results presented in the first paper. The other two papers are focused on fracturing of gelatin mixed with yeast and sugar, which generates CO2. Paper 3 describes the mechanism of fracture network formation during draining of CO2 out of a gelatine layer. Paper 4 focuses on the temporal evolution of the drainage network and the mechanism of fracture opening and closing. The last paper presents a study in which X-ray microtomography was used to characterize porosity distribution in weathered andesite samples. The scientific methods developed in this project have potential application in studying dehydration of sediments, formation of mud volcanoes, methane hydrate exploration and assessment, geological sequestration of carbon dioxide CO2 and hydraulic fracturing of unconventional reservoirs.

L’épithélium intestinal est un exemple de tissu qui a évolué pour former une barrière. Cette barrière limite le passage de produits toxiques d’agents pathogènes à partir de la lumière vers les tissus, tout en absorbant les nutriments, électrolytes et l'eau nécessaire à l'hôte. Les jonctions serrées sont des structures qui limitent le passage de la matière à travers l'espace intercellulaire. La capacité de mesurer le transport à travers cette barrière est d'une importance capitale car elle fournit des renseignements sur l’état de celle-ci, révélatrice de certains états pathologiques, puisque la perturbation ou dysfonctionnement des jonctions serrées est souvent due à ou est un indicatif de toxicité ou de maladie. En outre, le degré d'intégrité de la barrière est un indicateur clé de la pertinence d'un modèle in vitro particulier pour une utilisation en toxicologie et screening de médicaments. L'avènement de l'électronique organique a créé une occasion unique pour connecter les mondes de l'électronique et de la biologie, à l'aide des dispositifs tels que le transistor électrochimique organique (OECT), qui fournisse un moyen très sensible pour détecter des courants ioniques. Ces dispositifs ont une sensibilité sans précédent, dans un format qui peut être produit en masse à faible coût.Le but de cette étude était d'intégrer une couche de cellules représentative de la barrière gastro intestinale avec des OECTs, pour créer des dispositifs qui permettent de détecter les perturbations de cette barrière d’une manière rapide et sensible. Cette technique a était démontrée pour être au minimum aussi sensible mais d’une rapidité supérieure que les techniques actuelles sur le marché. / In biological systems, different tissues have evolved to form a barrier. An example is the intestinal epithelium, consisting of a single layer of cells lining the wall of the stomach and colon. It restricts the passage of harmful chemicals or pathogens from the light into the tissue, while selectively absorbing the most nutrients, electrolytes and water are necessary for the host. Tight junctions are structures which limit the passage of the material through the space between the cells. The ability to measure the paracellular and transcellular transport is of vital importance because it provides a wealth of information on the state of the barrier, indicative of certain disease states , since the disruption or malfunction of the structures involved in the transport through the tissue barrier is often caused or is indicative of toxicity or disease. In addition, the degree of integrity of the barrier is a key indicator of the relevance of a particular model in vitro for use in toxicology and drug screening. The advent of organic electronics has created a unique opportunity to connect the worlds of electronics and biology, using devices such as organic electrochemical transistor (OECT), which provides a very sensitive way to detect ionic currents. These devices have unprecedented sensitivity in a format that can be mass produced at low cost.The purpose of this study was to integrate a monolayer of cells representative of the gastro intestinal barrier with OECTs , to create devices that detect disruptions of the barrier in a timely and sensitive manner. This technique was demonstrated to be at least as sensitive, but a higher speed than current techniques on the market

INTRODUÇÃO: O carcinoma escamoso (CEC) de vulva é um tumor ginecológico de baixa frequência, cuja incidência aumenta com o passar dos anos. Entre as vias patogenéticas, destaca-se a neoplasia intraepitelial vulvar (NIV) diferenciada, que está relacionada com o líquen escleroso (LE). A maioria dos estudos publicados comparou o CEC vulvar com LE no âmbito morfológico apenas. Poucos avaliaram estas afecções em relação à biologia molecular, e nenhum avaliou o papel da junção intercelular (TJ). Nosso objetivo foi analisar a expressão de claudinas (proteínas atuantes na TJ) e do p53 nestas doenças. CASUÍSTICA E MÉTODOS: avaliamos o produto do oncogene TP53 e a expressão das claudinas 1, 2, 3, 4, 5, 7 e 11 em amostras de tecido vulvar humano de três grupos de pacientes: LE, CEC isolado (ICEC) e grupo controle. RESULTADOS: As claudinas 1, 2, 3 e 4 foram expressas igualmente nos três grupos. As claudinas 5, 7 e 11 não foram expressas nos grupos LE e ICEC, estando presentes apenas no grupo controle. Esta diferença foi significativa apenas para as claudinas 7 (p=0,013) e 11 (p=0,001). A proteína p53 foi mais expressa no grupo ICEC, seguida pelo LE e pelo grupo controle (p=0,017). CONCLUSÕES: As claudinas 5, 7 e 11 não se expressaram nos casos de LE e/ou ICEC. As claudinas 7 e 11 foram expressas apenas no grupo controle. Houve perda da expressão das claudinas 7 e 11 nos grupos com doença (LE e ICEC), em comparação ao grupo controle. Não houve diferença na expressão de claudinas entre os grupos LE e ICEC. Observou-se presença de p53 nos grupos estudados, cuja distribuição variou conforme o grupo analisado. Esta expressão foi maior no grupo ICEC, seguido pelo LE e, menor, no grupo controle / AIMS: Vulvar squamous cell carcinoma (SCC) is a rare gynaecologic cancer. Vulvar SCC has been shown to develop from vulvar intraepithelial neoplasias (VINs), which are related to lichen sclerosus (LS). Most studies to date have compared vulvar SCC to LS only morphologically, but no detailed molecular analysis has been performed. Our objective was to compare claudin and p53 expression in these diseases and determine if there was an association with expression and vulvar SCC progression. METHODS: Immunohistochemical analysis was performed in order to determine expression of p53 and claudin 1, 2, 3, 4, 5, 7, and 11 in human vulvar tissue samples from LS, SCC, and control patients. RESULTS: Claudin 1, 2, 3, 4, and 5 were expressed comparably in the three groups. Claudin 7 and 11 expression was significantly decreased in LS and SCC samples (p=0,013 and 0,001, respectively) compared with the control group. Expression of p53 was significantly increased in SCC and LS patient samples compared with the control group (p=0,017). CONCLUSIONS: Claudins 7 and 11 were expressed only in control group. There was loss of expression of claudins 7 and 11 in the disease groups (LS and/or SCC), comparing to control group. However, there was no significant difference in expression of any of the claudins between the LS and SCC samples. Furthermore, p53 expression is higher in SCC patients and lower in control group. However, expression of p53 did not vary between samples from isolated LS (ILS) and LS associated with SCC (ALSSCC) patients

INTRODUÇÃO: O carcinoma escamoso (CEC) de vulva é um tumor ginecológico de baixa frequência, cuja incidência aumenta com o passar dos anos. Entre as vias patogenéticas, destaca-se a neoplasia intraepitelial vulvar (NIV) diferenciada, que está relacionada com o líquen escleroso (LE). A maioria dos estudos publicados comparou o CEC vulvar com LE no âmbito morfológico apenas. Poucos avaliaram estas afecções em relação à biologia molecular, e nenhum avaliou o papel da junção intercelular (TJ). Nosso objetivo foi analisar a expressão de claudinas (proteínas atuantes na TJ) e do p53 nestas doenças. CASUÍSTICA E MÉTODOS: avaliamos o produto do oncogene TP53 e a expressão das claudinas 1, 2, 3, 4, 5, 7 e 11 em amostras de tecido vulvar humano de três grupos de pacientes: LE, CEC isolado (ICEC) e grupo controle. RESULTADOS: As claudinas 1, 2, 3 e 4 foram expressas igualmente nos três grupos. As claudinas 5, 7 e 11 não foram expressas nos grupos LE e ICEC, estando presentes apenas no grupo controle. Esta diferença foi significativa apenas para as claudinas 7 (p=0,013) e 11 (p=0,001). A proteína p53 foi mais expressa no grupo ICEC, seguida pelo LE e pelo grupo controle (p=0,017). CONCLUSÕES: As claudinas 5, 7 e 11 não se expressaram nos casos de LE e/ou ICEC. As claudinas 7 e 11 foram expressas apenas no grupo controle. Houve perda da expressão das claudinas 7 e 11 nos grupos com doença (LE e ICEC), em comparação ao grupo controle. Não houve diferença na expressão de claudinas entre os grupos LE e ICEC. Observou-se presença de p53 nos grupos estudados, cuja distribuição variou conforme o grupo analisado. Esta expressão foi maior no grupo ICEC, seguido pelo LE e, menor, no grupo controle / AIMS: Vulvar squamous cell carcinoma (SCC) is a rare gynaecologic cancer. Vulvar SCC has been shown to develop from vulvar intraepithelial neoplasias (VINs), which are related to lichen sclerosus (LS). Most studies to date have compared vulvar SCC to LS only morphologically, but no detailed molecular analysis has been performed. Our objective was to compare claudin and p53 expression in these diseases and determine if there was an association with expression and vulvar SCC progression. METHODS: Immunohistochemical analysis was performed in order to determine expression of p53 and claudin 1, 2, 3, 4, 5, 7, and 11 in human vulvar tissue samples from LS, SCC, and control patients. RESULTS: Claudin 1, 2, 3, 4, and 5 were expressed comparably in the three groups. Claudin 7 and 11 expression was significantly decreased in LS and SCC samples (p=0,013 and 0,001, respectively) compared with the control group. Expression of p53 was significantly increased in SCC and LS patient samples compared with the control group (p=0,017). CONCLUSIONS: Claudins 7 and 11 were expressed only in control group. There was loss of expression of claudins 7 and 11 in the disease groups (LS and/or SCC), comparing to control group. However, there was no significant difference in expression of any of the claudins between the LS and SCC samples. Furthermore, p53 expression is higher in SCC patients and lower in control group. However, expression of p53 did not vary between samples from isolated LS (ILS) and LS associated with SCC (ALSSCC) patients

<p>Our knowledge of the fine structure of the Human Spiral Ganglion (HSG) is still inadequate and new treatment techniques for deafness using electric stimulation, call for further information and studies on the neuronal elements of the human cochlea. This thesis presents results of analyses of human cochlear tissue and specimens obtained during neurosurgical transpetrosal removal of life-threatening meningeomas. The use of surgical biopsies produced a well-preserved material suitable for ultrastructural and immunohistochemical studies on the human inner ear. The SG was studied with respect to fine structure, using TEM technique and the immunostaining pattern of synaptophysin, which is an integral membrane protein of neuronal synaptic vesicles. The immunostaining patterns of the tight junctional protein ZO-1 and the gap junctional proteins Cx26 and Cx43 in the human cochlea were also studied. The ultrastructural morphology revealed an absence of myelination pattern in the HSG, thus differing from that in other species. Furthermore, formation of structural units as well as signs of neural interaction between the type 1 neurons could be observed. Type 1 cells were tightly packed in clusters, sharing the ensheathment of Schwann cells. The cells frequently made direct physical contact in Schwann cell gaps in which membrane specializations appeared. These specializations consisted of symmetrically or asymmetrically distributed filamentous densities along the apposed cell membranes. The same structures were also present between individual unmyelinated nerve fibres and the type 1 cells. Synapses were observed on the type 2 neurons, with nerve fibres originating from the intraganglionic spiral bundle. Such synapses, though rare, were also observed on the type 1 cells. The ultrastructural findings were confirmed by the synaptophysin study. A 3-D model of a Schwann cell gap between two type 1 cells was constructed, describing the distribution pattern of membrane specializations. In the immunohistochemical studies on the human cochlea, ZO-1 was expressed in tissues lining scala media, thus contributing to the formation of a closed compartment system, important for the maintenance of the specific ionic composition of the endolymph. Protein Cx26 could be identified in non-sensory epithelial cells of the organ of Corti, in connective tissue cells of the spiral ligament and spiral limbus, as well as in the basal and intermediate cell layers of stria vascularis. Cx26 in this region may be involved in the recycling of potassium. Protein Cx43 stained weakly in the spiral ligament, but intense staining in the SG may indicate that gap junctions exist between these neurons. A different functional role for the HSG can be assumed from the morphological characteristics and the signs of a neural interaction between the SG cells. This might be relevant for neural processing mechanisms in speech coding and could have implications for cochlear implant techniques.</p>

Thesis (M.Ing. (Mechanical Engineering))--North-West University, Potchefstroom Campus, 2007.

The search for new solutions for the generation of energy is becoming more and more important for our future. Big arguments and disagreements on e.g. the questions of gas transport or the dependence on energy supplied by other countries raise demands on the development of new forms of alternative energy resources. Wave power is one of the main sources of renewable energy due to the high power density stored in ocean waves. Nevertheless, the dynamic forces of waves are so large that serious questions popped up on how to design a system which could work even in an unfavourable wave climate or could at least retain working capabilities after big storms without significant damages. This thesis studies the reliability of the mechanical parts of a linear direct driven permanent magnet generator. The results of offshore experiment where strain gauge sensors instrumented on the capsule and the inner framework structure are presented. Stress estimation analyses using strain gauges are carried out. A method for measuring forces and moments in the mechanical structure of the WEC is developed. Evaluation of the lateral force acting on the outer structure is a key factor for the design and construction of the WEC. A method for the measurement of the lateral force acting on the capsule has been developed. A study of the inclination angle between the Wave Energy Converter and the floating buoy has been carried out. The aim of this work is to contribute to the development of wave energy conversion system, and especially to the estimation of structural loads which are important for the survivability of the system under hard sea states. This work is a step that may influence future design of wave energy devices in terms of material aspect, survivability in a hard wave climate and cost-effective renewable energies.

The large amount of produced oil and gas come from conventional resources all over the world and these resources are being depleted rapidly. This fact and the increasing oil and gas prices force the producing countries to find and search for new methods to recover more oil and gas. In order to meet the demand, the oil and gas industry has been turning towards to unconventional oil and gas reservoirs which become more popular every passing day. In recent years, they are seriously considered as supplementary to the conventional resources although these reservoirs cannot be produced at an economic rate or cannot produce economic volumes of oil and gas without assistance from massive stimulation treatments, special recovery processes or advanced technologies. The vast increase in demand for petroleum and gas has encouraged the new technological development and implementation. A wide range of technologies have been developed and deployed since 1980. With the wellbore technology, it is possible to make use of highly deviated wellbores, extended reach drilling, horizontal wells, multilateral wells and so on. All of the new technologies and a large number of new innovations have allowed development of increasingly complex economically marginal fields where shale gas and coal bed methane are found. In this study, primary target is to compare different production methods in order to obtain better well performance and improved production from different types of reservoirs. It is also be given some technical information regarding the challenges such as hydraulic fracturing and multilateral well configuration of the unconventional gas reservoir modeling and simulation. With the help of advances in algorithms, computer power, and integrated software, it is possible to apply and analyze the effect of the different well trajectories such as vertical, horizontal, and multilateral well on the future production performance of coal bed methane, shale gas, and tight gas reservoirs. A commercial simulator will be used to run the simulations and achieve the best-case scenarios. The study will lead the determination of optimum production methods for three different reservoirs that are explained above under the various circumstances and the understanding the production characteristic and profile of unconventional gas systems.

The so-called "Arps" rate decline relations are by far the most widely used tool for assessing oil and gas reserves from rate performance. These relations (i.e., the exponential and hyperbolic decline relations) are empirical where the starting point for their derivation is given by the definitions of the "loss ratio" and the "derivative of the loss ratio", where the "loss ratio" is the ratio of rate data to derivative of rate data, and the "derivative of the loss ratio" is the "b-parameter" as defined by Arps [1945]. The primary goal of this work is the interpretation of the b-parameter continuously over time and thus the better understanding of its character. As is shown below we propose "monotonically decreasing functional forms" for the characterization of the b-parameter, in addition to the exponential and hyperbolic rate decline relations, where the b-parameter is assumed to be zero and constant, respectively. The proposed equations are as follow: b(t)=constant (Arps' hyperbolic rate-decline relation), []tbbtb10exp)(-bt= (exponential function), (power-law function), 10)(btbtb=)/(1)(10tbbtb+= (rational function). The corresponding rate decline relation for each case is obtained by solving the differential equation associated with the selected functional for the b-parameter. The next step of this procedure is to test and validate each of the rate decline relations by applying them to various numerical simulation cases (for gas), as well as for field data cases obtained from tight/shale gas reservoirs. Our results indicate that b-parameter is never constant but it changes continuously with time. The ultimate objective of this work is to establish each model as a potential analysis/diagnostic relation. Most of the proposed models yield more realistic estimations of gas reserves in comparison to the traditional Arps' rate decline relations (i.e., the hyperbolic decline) where the reserves estimates are inconsistent and over-estimated. As an example, the rational b-parameter model seems to be the most accurate model in terms of representing the character of rate data; and therefore, should yield more realistic reserves estimates. Illustrative examples are provided for better understanding of each b-parameter rate decline model. The proposed family of rate decline relations was based on the character of the b-parameter computed from the rate-time data and they can be applied to a wide range of data sets, as dictated by the character of rate data.

This work was conducted to help understand a premature and irregular water breakthrough which resulted from a waterflooding project in the increment II region of Haradh oilfield in Saudi Arabia using different geophysical methods. Oil wells cannot sustain the targeted oil production rates and they die much sooner than expected when water enters the wells. The study attempted to identify fracture systems and their role in the irregular water sweep. Single-well acoustic migration imaging (SWI), walkaround vertical seismic profiling (VSP) and cross-dipole shear wave measurements were used to detect anisotropy caused by fractures near and far from the borehole. The results from all the different methods were analyzed to understand the possible causes of water fingering in the field and determine the reasons for discrepancies and similarities of results of the different methods. The study was done in wells located in the area of the irregular water encroachment in Haradh II oilfield. Waterflooding was performed, where water was injected in the water injector wells drilled at the flanks of Harahd II toward the oil producer wells. Unexpected water coning was noticed in the west flank of the field. While cross-dipole and SWI measurements of a small-scale clearly identify a fracture oriented N60E in the upper tight zone of the reservoir, the VSP measurements of a large-scale showed a dominating fracture system to the NS direction in the upper highpermeability zone of the same reservoir. These results are consistent with the directions of the three main fracture sets in the field at N130E, N80E and N20E, and the direction of the maximum horizontal stress in the field varies between N50E and N90E. Results suggested that the fracture which is detected by cross-dipole at 2 to 4 ft from the borehole is the same fracture detected by SWI 65 ft away from the borehole. This fracture was described using the SWI as being 110 ft from top to bottom, having an orientation of N60E and having an angle of dip of 12° relative to the vertical borehole axis. The detected fracture is located in the tight zone of the reservoir makes a path for water to enter the well from that zone. On the Other hand, the fractures detected by the large-scale VSP measurements in the NS direction are responsible for the high-permeability in the upper zone of the reservoir.