This study on the relations between tourism policies and the tourism development conditions in the Eastern Cape of South Africa is a contribution to the on-going research projects on the transformation that has taken place in the post-1994 development process of South Africa. One theme associated with these studies relates to the important roles that have been played by public bodies in influencing the nature of the transformation in the development of South Africa. The study is based on the expectation that the current policies can open up more investments and other opportunities in the tourism sector to the benefit of the provincial economy. To find out the concrete nature of the changes associated with the current tourism development policies, data collection was organized from six groups of stakeholders in the tourism sector of the 39 local municipalities of the Eastern Cape Province. The use of factor analysis led to the identification of five hierarchies or clusters indicating the levels of inequalities in the tourism development of the province. To obtain an understanding of the processes behind the observed inequalities, the stakeholders were given the opportunity to disclose their concrete activities as they related to the policies. The findings indicate that generally, much has changed since 1994, as a result of the levelling of the playing field in the tourism sector for both the producers and consumers in the industry. It is recommended, however, that measured in terms of the maximum benefits that could be derived from the provincial tourism sector, the government officials associated with the tourism sector in particular need to provide more effective leadership in the implementation of the current tourism development policies. The thrust of the study is that the tourism policies in the Eastern vii Cape can only generate the maximum positive benefits if, inter alia, all the stakeholders in the tourism industry are empowered to produce at their maximum. / Geography / (D. Litt et Phil. (Geography))

Ce mémoire présente l'imbrication des éléments hydroélectrique au sein du paysage de la ville de Sherbrooke entre 1880 et 1930. Nous cherchons à comprendre comment les nouveaux éléments énergétiques s'intègrent à celui-ci et comment la population sherbrookoise se représente ces espaces transformés. Comment le paysage hydroélectrique, en tant qu'espace vu et vécu, interagit-il avec la société sherbrookoise? À l'intérieur de cette problématique générale, nous chercherons à répondre à trois sous-questions. Quelles sont les transformations physiques et matérielles engendrées par l'implantation de cette nouvelle technologie hydroélectrique sur le paysage urbain sherbrookois? Qu'est-ce que les différents groupes valorisent au sein du paysage? Quel sens prennent ces nouveaux paysages pour les Sherbrookois? En effet, l'étude du développement rapide du potentiel hydroélectrique de la rivière Magog nous amène à démontrer que l'exploitation des ressources énergétiques a profondément transformé le paysage de Sherbrooke : vastes modifications du tracé des cours d'eau, apparition de bâtiments spécifiques à la production énergétique, structures de distributions et infrastructures permettant l'illumination de la ville. Nous avançons aussi que différents groupes d'individus ne valorisent pas les mêmes éléments paysagers. Finalement, nous proposons que le regard porté par les citoyens sur le paysage énergétique est tributaire de l'usage qu'ils en font. Comme la fonction des éléments énergétiques paysagers évolue, le regard qu'ils portent vers ces structures se transforme lui aussi.

La voie KRAS/BRAF/MEK/ERK MAP Kinase joue un rôle clé dans le contrôle de la prolifération des cellules épithéliales intestinales normales et cancéreuses. En effet, on retrouve des mutations du gène KRAS dans près de 35 à 40% des cancers colorectaux et une mutation du gène BRAF dans 10 à 15% des cas. Ces mutations de type gain-de-fonction sont mutuellement exclusives, ce qui suggère que la signalisation MEK/ERK qui est en aval de BRAF joue possiblement un rôle crucial dans le développement de plus de 60% des cancers colorectaux. Notre laboratoire a d’ailleurs rapporté que l’expression d’une forme mutante hyperactive de MEK1 est suffisante pour induire la transformation des cellules épithéliales intestinales normales en culture. Cette transformation est caractérisée par une transition épithélium-mésenchyme (EMT) conférant aux cellules des capacités tumorales, invasives et métastatiques. Afin de mieux comprendre les mécanismes moléculaires impliqués dans les effets transformant de MEK1, une analyse comparative par micropuces d’ADN (Affymetrix) a été effectuée et celle-ci a montré que le gène codant pour la protéine CDK8, une kinase dépendante des cyclines, est un des gènes les plus induits (12 fois) par l’hyperactivation de MEK1. Ce résultat suggèrerait l’implication de CDK8 dans l’oncogenèse colorectale induite par l’hyperactivation de la voie KRAS/MAP Kinase. De manière intéressante, nous avons d’abord mis en évidence que CDK8 était surexprimée dans des tumeurs de patients atteints de cancer colorectal de différents stades ainsi que dans des lignées cancéreuses colorectales humaines. Parmi ces lignées cellulaires analysées, nous avons mis en évidence que cette surexpression était en partie dépendante de l’activité MEK. Nous avons aussi confirmé la surexpression de CDK8 dans des lignées de cellules épithéliales intestinales de rat exprimant les oncogènes KRAS ou BRAF ou le mutant de MEK1 constitutivement actif. La baisse d’expression de CDK8 par l’utilisation d’un shARN a révélé que CDK8 contribue à l’hyperprolifération cellulaire ainsi qu’à la croissance en indépendance d’ancrage induite par l’expression du mutant hyperactif de MEK1. De plus, la baisse d’expression de CDK8 atténue le phénotype fibroblastique des cellules transformées par l’oncogène BRAF ou le mutant de MEK1 constitutivement actif, qui exhibent un phénotype plus épithélial. Nous avons pu mettre en évidence que CDK8 serait impliqué dans l’expression de gènes liés à la morphologie cellulaire tel que Snail1, Snail2 et Gem. Nos résultats montrent donc que CDK8 contribue au potentiel oncogénique de la voie MAP Kinase dans les cellules épithéliales intestinales en modulant leurs capacités prolifératives et leur transformation morphologique.

Just like in other national legislatures in recent years, women have increased in numbers in Uganda, owing mostly to the introduction of affirmative action policies. These measures are regarded as fast track approaches to counter previous historical injustices and imbalances. However, these developments, which also reflect transposition in the social positioning of women from a marginal and probably limited outlook, to a broader, public and visible status in the public sphere, come with various challenges. The constraints are attributed to lack of adjustments within the organizational norms and procedures. Thus the main questions addressed by the study are: What happens when this previously less represented group becomes part of the mainstream? What are the implications in information access, information communication and information use? How can this inform us about the overall process of integration and social transformation? What information possibilities can women exploit to gain a more central place in mainstream politics? One of the assumptions is that access to and use of information is essential to full integration and in occupying a dominant position in the political environment which would consequently transform governance. The thesis is based on qualitative in-depth interviews and observations of legislators and non legislators with strong connections to Parliamentarians’ tasks. The findings reveal that a legislator’s versatility, world outlook and social positioning within the Parliamentary structures greatly improves ability to acquire and use information and possibly a legislator’s capability to influence national policy making. Women face challenges at two levels; the social and political context. There are possibilities of change through their own network. / Akademisk avhandling som med tillstånd av samhällsvetenskapliga fakulteten vid Göteborgs universitet för vinnande av doktorsexamen framläggs till offentlig granskning kl. 13.00 tisdagen den 29 september 2009 i sal E310, Högskolan i Borås.

Database integration is one of the research areas that have gained a lot of attention from researcher. It has the goal of representing the data from different database sources in one unified form. To reach database integration we have to face two obstacles. The first one is the distribution of data, and the second is the heterogeneity. The Web ensures addressing the distribution problem, and for the case of heterogeneity there are many approaches that can be used to solve the database integration problem, such as data warehouse and federated databases. The problem in these two approaches is the lack of semantics. Therefore, our approach exploits the Semantic Web methodology. The hybrid ontology method can be facilitated in solving the database integration problem. In this method two elements are available; the source (database) and the domain ontology, however, the local ontology is missing. In fact, to ensure the success of this method the local ontologies should be produced. Our approach obtains the semantics from the logical model of database to generate local ontology. Then, the validation and the enhancement can be acquired from the semantics obtained from the conceptual model of the database. Now, our approach can be applied in the generation phase and the validation-enrichment phase. In the generation phase in our approach, we utilise the reverse engineering techniques in order to catch the semantics hidden in the SQL language. Then, the approach reproduces the logical model of the database. Finally, our transformation system will be applied to generate an ontology. In our transformation system, all the concepts of classes, relationships and axioms will be generated. Firstly, the process of class creation contains many rules participating together to produce classes. Our unique rules succeeded in solving problems such as fragmentation and hierarchy. Also, our rules eliminate the superfluous classes of multi-valued attribute relation as well as taking care of neglected cases such as: relationships with additional attributes. The final class creation rule is for generic relation cases. The rules of the relationship between concepts are generated with eliminating the relationships between integrated concepts. Finally, there are many rules that consider the relationship and the attributes constraints which should be transformed to axioms in the ontological model. The formal rules of our approach are domain independent; also, it produces a generic ontology that is not restricted to a specific ontology language. The rules consider the gap between the database model and the ontological model. Therefore, some database constructs would not have an equivalent in the ontological model. The second phase consists of the validation and the enrichment processes. The best way to validate the transformation result is to facilitate the semantics obtained from the conceptual model of the database. In the validation phase, the domain expert captures the missing or the superfluous concepts (classes or relationships). In the enrichment phase, the generalisation method can be applied to classes that share common attributes. Also, the concepts of complex or composite attributes can be represented as classes. We implement the transformation system by a tool called SQL2OWL in order to show the correctness and the functionally of our approach. The evaluation of our system showed the success of our proposed approach. The evaluation goes through many techniques. Firstly, a comparative study is held between the results produced by our approach and the similar approaches. The second evaluation technique is the weighting score system which specify the criteria that affect the transformation system. The final evaluation technique is the score scheme. We consider the quality of the transformation system by applying the compliance measure in order to show the strength of our approach compared to the existing approaches. Finally the measures of success that our approach considered are the system scalability and the completeness.

Recent European legislation has enforced a reduction in the use of animal models for safety assessment purposes and carcinogenicity testing. The Syrian hamster embryo cell transformation assay (SHE CTA) has been proposed as a suitable animal alternative, but its implementation into test batteries has been delayed. This is due to concerns regarding the assay’s endpoint subjectivity and, moreover, the model’s relevance to carcinogenicity remains mostly unexplored. Senescence is an essential barrier against uncontrolled cell proliferation and its evasion is necessary for clonal evolution and tumour development. Carcinogenesis can be modelled by reproducing underlying mechanisms leading to senescence bypass. In this project, the SHE CTA was performed using the known mutagen and human carcinogen, benzo(a)pyrene, and the resulting SHE colonies were analysed. It was found that morphological transformation (MT) does not guarantee senescence bypass and cell immortalisation, but increases the likelihood of MT-derived cells subsequently acquiring unlimited growth potential. A limited number (between 10 and 20 %) of MT colonies produced cell clones capable of sustained proliferation and in most cases secondary events were necessary for the evasion of senescence barriers. With regard to mechanisms, p53 point mutations were present in 30 % of immortal B(a)P-induced MT colony-derived cells and located within the protein’s DNA binding domain. No p16 mutations were identified. Expression of p16 mRNA was commonly silenced or markedly reduced by a combination of mechanisms including monoallelic deletion, promoter methylation and BMI-1 overexpression. Taking advantage of the recently available Syrian hamster genomic sequence information generated by the Broad Institute, the coding regions of the Syrian hamster CDKN2A/B locus were shown to have good homology to human nucleotide sequences and confirmed the exonic structures of SH p16, ARF and p15. The findings further implicate the importance of p16 in regulating senescence while providing a molecular evaluation of SHE CTA-derived MT clones.

The things we have an intimate connection to, handle, collect, and move around with us, are treasures that we need to hold on to. They are important in coping with the balance of movement and stability in a fast-changing world. This project is an investigation of the relationship between people and objects through the making and handling of stones. I explore why and how we connect intimately with physical objects, how they become treasures to us and what it means. Through digging into the stones, connecting to their story of endurance, change and solidity, and eventually letting them go, I explore the role of touching and paying attention, in making and relating to objects and transformation. The stone objects are made from pieces of rock I have collected while travelling. All of them have been transformed by human hands before I picked them out, and many have been given out and then returned to me. Through this ongoing process of transformation and physical encounters, it is becoming clear to me that connection is not only about solidity and stillness, but also about being part of the transformation. Stone as a material is both solid and changing. I aim to make objects of stone that attract and encourage people to engage with them – to experience the pleasure and groundedness that slowing down, zooming in and getting in touch can offer.

No description available.

Thesis (MSc)--University of Stellenbosch, 2000. / ENGLISH ABSTRACT: Agrobacterium tumefaciens-mediated transformation technology has been used in a variety of applications throughout the fields of cellular and molecular plant biology as well as plant physiology. Research is conducted in order to extend this application range and overcome some of the intrinsic limitations of the Agrobacterium transformation system. Predominantly, these limitations can be attributed to the host range specificity of A. tumefaciens, as well as adverse effects induced on explant tissue by active plant defence mechanisms, triggered by the plant-pathogen-interaction. Typically, this active defence mechanism culminates in the hypersensitive response (HR), characterised by localised cell death and necrosis. Not all Agrobacterium species, however, share the same host range and some have evolved the ability to infect plant species not normally considered hosts of A. tumefaciens. This host range specificity can be exploited to extend the application of existing Agrobacterium transformation systems. In an attempt to establish an efficient transformation system for Vitis vinifera which, has proven very difficult to transform with A. tumefaciens, indigenous A. vitis strains have been evaluated as possible host-specific transformation agents. Strains of Agrobacterium vitis should be suitable for this type of endeavour, since they have evolved several unique characteristics directly linked to the infection of their hosts. These include the ability to utilise, tartrate, a host abundant carbon source, as well as the production of an acid polygalacturonase that could play a role during the infection process. The proposition that the evolution of A. vitis is a fairly recent event is also confirmed by the relatively little divergence observed between A. tumefaciens and A. vitis. In this study, a selection of A. vitis strains were evaluated in screenings designed to accentuate desirable traits in strains such as good infectivity of grapevine material (presumably an indicator of an efficient mechanism of gene transfer to be exploited in an engineered transformation system) as well as a favourable reaction (causing no necrosis) on grapevine somatic embryos. Two strains produced large tumours on grapevine cuttings and caused little necrosis on the somatic embryos. Significant variation in infectivity as well as callus necrosis was observed between the strains as well as in a genotype-specific manner on the host material. This genotypic-specific effect of either host or pathogen could be an indication of the degree of specialisation developed by plant pathogens to infect specific hosts. On the basis of these results, it was possible to select an A. vitis strain for further biochemical and genetic characterisation. Simple biochemical analysis classified the strain as an octopine strain. DNA-DNA hybridisation techniques combined with a plasmid walking technique resulted in the partial characterisation of the T-DNA of the selected A. vitis strain. A partial restriction enzyme map of the T-DNA was constructed and the T-DNA and flanking areas were cloned. Significant differences, most notably, the absence of a TB-area as well as the absence of the agrocinopine (aes) gene from the 5' area of the T-DNA, were observed. Partial sequencing data indicated the presence of at least four conserved T-DNA genes located on the TA-DNA, as well as the presence of three bacterial insertion (IS-)elements flanking the region. Two of these IS elements, both related to the IS 110 family of IS elements have not yet been reported in A. vitis. In fact, these two elements seem to be the 5' and 3' ends of a disrupted element and could therefore have played an evolutionary role in the development of this strain. This study provides fundamental background for the development of a more efficient transformation system specific for grapevine, exploiting same of-the unique characteristics of one of its pathogens, A. vitis. / AFRIKAANSE OPSOMMING: Agrobacterium tumefaciens-gebaseerde transformasiesisteme word in "n wye reeks van toepassings in die velde van sellulêre- en molekulêre plantbiologie asook plantfisiologie aangewend. Navorsing word voortdurend onderneem om die inherente beperkinge van die Agrobacterium-transformasiesisteem te oorkom en sodoende die toepassingsveld van die sisteem verder te verbreed. Die beperkinge tipies aan dié sisteem kan hoofsaaklik toegeskryf word aan die gasheerspesifisteit van A. tumeteciens, asook die negatiewe reaksies op eksplantmateriaal wat deur die plant se aktiewe verdedigingsmeganisme, soos ontlok deur die plant-patogeen interaksie, veroorsaak word. Hierdie aktiewe verdedigingsmeganisme lei gewoonlik tot In hipersensitiewe respons (HR) in die plant, wat deur gelokaliseerde selafsterwing en nekrose gekenmerk word. Alle Agrobacterium-spesies het egter nie almal dieselfde gasheerreeks nie en sommige rasse het as gevolg van evolusionêre ontwikkelings die vermoë verkry om plantspesies wat normaalweg buite die gasheerreeks van A. tumefaciens val, te infekteer. Hierdie tipe gasheerspesifisiteit kan uitgebuit word om die toepassingsmoontlikhede van bestaande Agrobacterium-transformasiesisteme te verbreed. In In poging om In effektiewe transformasiesisteem vir Vitis vinifera, In moeilik transformeerbare gewas, te ontwikkel, is inheemse rasse van Agrobacterium vitis ondersoek as moontlike gasheerspesifieke transformasie-agente. Rasse van A. vitis behoort uiters geskik te wees vir so "n toepassing, aangesien hulle verskeie unieke eienskappe, wat direk aan die infeksie van die gasheer gekoppel is, vertoon. Van hierdie eienskappe is onder meer die vermoë om tartraat, In koolstofbron volop in druifplante, te benut. A. vitis produseer verder ook In suur poligalaktorunase wat vermoedelik In rol in die infeksieproses speel. Die voorstel dat die evolusionêre ontwikkeling van A. vitis In redelike onlangse gebeurtenis is, word onderskryf deur die betreklike homogenisiteit met A. tumefaciens. In hierdie studie is "n groep A. vitis-rasse met behulp van siftingsprosedures wat daarop gemik is om gesogte eienskappe in rasse uit te wys, beoordeel. Die vermoë om druifplantmateriaal te infekteer (wat vermoedelik "n aanwyser van "n effektiewe meganisme van geenoordraging is wat in "n gemanipuleerde transformasiesisteem benut kan word), sowel as 'n gunstige reaksie (d.w.s geen nekrose) op druifplant somatiese embrio's is van die gesogte eienskappe waarvoor gesoek word. Twee rasse het groot tumors op druifplant-stingelsegmente veroorsaak terwyl hulle bykans geen weefselskade op somatiese embrio's geïnduseer het nie. Betekenisvolle verskille in infektiwiteit en in kallusnekrose is tussen die rasse sowel as in 'n genotipe-spesifieke-verhouding waargeneem. Hierdie genotipe-spesifieke effek, kenmerkend van óf die gasheer óf die patogeen, kan aanduidend wees van die vlak van spesialisasie wat heers by die infeksie van spesifieke gashere. Na aanleiding van bogenoemde resultate was dit moontlik om 'n A. vitis-ras te selekteer wat verder aan biochemiese en genetiese analises onderwerp kon word. Eenvoudige biochemiese analises het dit moontlik gemaak om die ras as oktopien te klassifiseer. DNA-DNA hibridisasietegnieke gekombineerd met 'n unieke plasmiedwandeltegniek het gelei tot die gedeeltelike karakterisering van die geselekteerde A. vitisras. In Gedeeltelike restriksie-ensiem (RE) kaart van die T-DNA kon gevolglik opgestel word. Die T-DNA en die aangrensende gedeeltes is boonop gekloneer. Betekenisvolle verskille, spesifiek die afwesigheid van In TB area, sowel as die afwesigheid van die agrosinopien-sintasegeen (acs) aan die 51-kant van die T-DNA, is waargeneem. Gedeeltelike basispaaropeenvolgingsdata het egter die teenwoordigheid van minstens vier gekonserveerde T-DNA-gene, asook die teenwoordigheid van drie bakteriese invoegingselemente (IS) aan weerskante van die area, geïdentifiseer. Twee van hierdie elemente, wat beide homologie vertoon met die IS110 familie van IS elemente, is nog nie vantevore in A. vitis aangetref nie. Dit wil boonop blyk of dié twee elemente die 51 - en 31 - areas van In onderbroke element vorm, wat dus In moontlike aanduiding is van hul potensiële rol in die evolusionêre ontwikkeling van die ras. Hierdie studie verskaf basiese inligting wat daartoe kan lei dat 'n doeltreffender transformasiesisteem spesifiek vir druifplante ontwikkel word deur van die unieke kenmerke van een van sy patogene, A. vitis, uit te buit.

The v-rel oncogene is the most efficiently transforming member of the Rel/NF-κB family of transcription factors. Identification of genes or signal transduction pathways that contribute to v-Rel transformation provide insight into the mechanisms of tumorigenesis by Rel/NF-κB proteins. In these studies, the contribution of TGF-β/Smad signaling to v-Rel transformation was assessed. TGF-β/Smad signaling regulates several cellular processes, including growth, differentiation, and apoptosis and has been implicated in a number of different cancers. Using microarray technology and Northern blot analysis, key components of the TGF-β/Smad pathway (tgf-β2 and tgf-β3 ligands, TGF-β type II receptor, and receptor-activated smad3) were identified with upregulated mRNA expression in v-Rel-transformed fibroblasts and lymphoid cells relative to control cells. A corresponding change in their protein levels was also observed. Further analysis revealed elevated levels of the phosphorylated, active form of Smad3, which correlated with its increased DNA-binding activity in v-Rel transformed cells. In contrast, the overexpression of c-Rel resulted in little to no alteration in the RNA and protein expression of members of the TGF-β/Smad pathway. Further studies demonstrated that elevated TGF-β/Smad signaling is required for the transforming ability of v-Rel. Blocking TGF-β signaling with a kinase inhibitor of TGF-β type I receptor inhibited the activation of Smad3 and dramatically reduced the ability of v-Rel transformed cells to form colonies in soft agar. Overexpression of a constitutively active form of Smad3 in the inhibitor-treated cells restored their ability to form colonies in soft agar close to the levels seen in untreated cells. Additional experiments with dominant negative Smad3 also revealed its ability to hinder the oncogenic potential of v-Rel. In complementary experiments, a stimulatory effect on v-Rel transformation was observed with cells treated with recombinant TGF-β2 ligand or overexpressed with wild-type Smad3. Taken together, these studies demonstrate that TGF-β signaling is crucial for the transformation potential of v-Rel and is primarily mediated by Smad3 activity. / text