Effective gages to measure skin friction and heat transfer have been established over decades. One of the most important criteria in designing such a gage is the physical size of the gage to minimise the interference of the flow, as well as the mass of these devices. The combined measurement of skin friction and heat flux using one single gage on the other hand, present unique opportunities and with it, unique technical problems. The objective of this study is therefore to develop a cost-effective single gage that can be used to measure both skin friction and heat flux. The method proposed in this study is to install a coaxial thermocouple into an existing skin friction gage to measure the unsteady temperature on the surface of the gage. By using the temperature history and a computer program the heat flux through the surface can be obtained through an iterative guessing method. To ensure that the heat flux through the gage is similar to the heat flux through the rest of the surface, the gage is manufactured of a material very similar to the rest of the surface. Walker developed a computer program capable of predicting the heat flux through a surface from the measured surface temperature history. The program is based on an inverse approach to calculate the heat flux through the surface. The biggest advantages of this method are its stability and the small amount of noise induced into the system. The drawback of the method is that it is limited to semi-infinite objects. For surfaces with a finite thickness, a second thermocouple was installed into the system some distance below the first thermocouple. By modifying the computer program these two unsteady temperatures can be used to predict the heat flux through a surface of finite thickness. As part of this study, the effect of noise induced by the Cook-Felderman technique, found in the literature were investigated in detail and it was concluded that the method proposed in this study is superior to this Cook-Felderman method. Heat flux measurements compared well with measurements recorded with heat flux gages. In all cases evaluated the difference was less than 20%. It can therefore be concluded that heat flux gages on their own can measure surface heat flux very accurately. These gages are however too large to install in a skin-friction gage. The method introduced in this study is noisier than the heat flux gages on their own, but the size which is very important, is magnitudes smaller when using a coaxial thermocouple, to measure the surface temperature history. / Master of Science

In recent years government, academia and industry have been pursuing fuel cell technology as an alternative to current power generating technologies. The automotive industry has targeted fuel cell technology as a potential alternative to internal combustion engines. The goal of this research is to understand and quantify the impact and effects of low temperature operation has on the performance and efficiency of vehicle fuel cell systems through modeling. More specifically, this work addresses issues of the initial thermal transient known to the automotive community as "cold-start" effects. Cold-start effects play a significant role in power limitations in a fuel cell vehicle, and may require hybridization (batteries) to supplement available power. A fuel cell system model developed as part of this work allows users to define the basic thermal fluid relationships in a fuel cell system. The model can be used as a stand-alone version or as part of a complex fuel cell vehicle model. Fuel cells are being considered for transportation primarily because they have the ability to increase vehicle energy efficiency and significantly reduce or eliminate tailpipe emissions. A proton exchange membrane fuel cell is an electrochemical device for which the operational characteristics depend heavily upon temperature. Thus, it is important to know how the thermal design of the system affects the performance of a fuel cell, which governs the efficiency and performance of the system. This work revealed that the impact on efficiency of a cold-start yielded a 5 % increase in fuel use over a regulated drive cycle for the converted sport utility vehicle. The performance of the fuel cell vehicle also suffered due to operation at low temperatures. Operation of the fuel cell at 20 C yielded only 50% of the available power to the vehicle system. / Master of Science

The thesis presents an intermediate modelling approach to study transient behaviour of vehicle systems, with emphasis put on simplified yet accurate representation of important system elements. A representative non-linear vehicle model is developed in MA TLAB/Simulink environment, where non-linear characteristics of tyre, suspension and braking system are included to capture the dynamic behaviour of a vehicle under transient conditions. The novel aspect of this work is the application of a representative full vehicle-tyre-ABS integrated set-up to study the complicated interaction between tyre and anti-lock braking, under a range of demanding operating conditions, including combined cornering and braking. The modelling methodology involves development of low end vehicle models, based on the Newton-Euler formulation. Subsequently, an intermediate vehicle model is devised, where more details are incorporated such as additional DOF to capture the sprung mass motion in space, along with its non-linear interactions with the un-sprung masses, large angle effects, kinematics of steering/wheels and an appropriate tyre model suitable for transient manoeuvres. Particular attention is paid to the suspension system modelling, through inclusion of non-linear effects in springs, dampers, bump-stops, and anti-roll bars, along with the jacking and anti-dive effects using the virtual work method. The model also incorporates a hydraulic brake model, based on the reduced order brake system dynamics for realistic simulation of the braking manoeuvres. A complex multi-body ADAMS/Chassis model, with much greater level of detail, has also been established to extensively compare and enhance the realistic behaviour of the intermediate vehicle model. During the simulation exercise, the intermediate vehicle model has shown good agreement with the complex ADAMS model, thus justifying the accurate representation of vehicle.non-linear characteristics, particularly the suspension system. The realistic behaviour of the vehicle model is further ascertained with a reliable GPS enabled test vehicle, by performing number of manoeuvres on test tracks, including combined cornering and braking. A representative 4-channel conventional ABS system is modelled and integrated in the intermediate vehicle model. The ABS adopts generic peak seeking approach, employing wheel deceleration and brake slip as control variables. External braking inputs, in form of stepped pressure pulses, are also separately used to represent the transient braking system dynamics. In the current work, different transient tyre models based on the single point contact approach and using Magic Formula steady-state characteristics are applied, while studying the influence of their dynamic behaviour on the ABS system. By employing a representative ABS system in a multi-body vehicle model and considering the particularly demanding situation of combined braking I cornering, it is shown that the models which are adequate for pure braking might struggle when the complicated full vehicle dynamics are excited. It is shown that the first order relaxation length approach may not be sufficient to fully satisfy the requirements of an ABS braking, especially if the relaxation length is not modelled as a variable dependent on tyre slip. In comparison, the modelling approach, where the carcass compliances and contact patch properties are explicitly represented, can handle the oscillatory tyre behaviour associated with ABS braking, in a far more accurate manner. In comparison to the earlier studies, which were mostly conducted for straight-line braking, this thesis stresses the fact that the tyre behaviour can be influenced by the complex interaction of handling and braking, and hence the effect should be captured while investigating or evaluating the performance of a tyre model in relation with ABS simulation.

The purpose of this research is to develop a method of making system equivalents for the Real Time Digital Simulator (RTDS), which should enhance its capability of simulating large power systems. The proposed equivalent combines a Frequency Dependent Network Equivalent (FDNE) for the high frequency electromagnetic transients and a Transient Stability Analysis (TSA) type simulation block for the electromechanical transients. The frequency dependent characteristic for FDNE is obtained by curve-fitting frequency domain admittance characteristics using the Vector Fitting method. An approach for approximating the frequency dependent characteristic of large power networks from readily available typical power-flow data is also introduced. A new scheme of incorporating TSA solution in RTDS is proposed. This report shows how the TSA algorithm can be adapted to a real time platform. The validity of this method is confirmed with examples, including the study of a multi in-feed HVDC system based network.

The purpose of this research is to develop a method of making system equivalents for the Real Time Digital Simulator (RTDS), which should enhance its capability of simulating large power systems. The proposed equivalent combines a Frequency Dependent Network Equivalent (FDNE) for the high frequency electromagnetic transients and a Transient Stability Analysis (TSA) type simulation block for the electromechanical transients. The frequency dependent characteristic for FDNE is obtained by curve-fitting frequency domain admittance characteristics using the Vector Fitting method. An approach for approximating the frequency dependent characteristic of large power networks from readily available typical power-flow data is also introduced. A new scheme of incorporating TSA solution in RTDS is proposed. This report shows how the TSA algorithm can be adapted to a real time platform. The validity of this method is confirmed with examples, including the study of a multi in-feed HVDC system based network.

In dieser Arbeit wurde ein Arbeitsablauf entwickelt, um ein möglichst realistisches, biomechanisches Modell eines menschlichen Schädelknochens anhand eines Patienten-CT Datensatzes zu erstellen. Mit diesem Modell konnten Experimente aus der Literatur realistisch nachgestellt und anschließend der Mechanismus einer Orbitawandfraktur genauer untersucht werden. Es konnte gezeigt werden, dass das entwickelte Schädelmodell als Alternative für experimentelle biomechanische Untersuchungen verwendet werden kann. Somit sind eine Vielzahl parametrischer biomechanischer Studien möglich, ohne dabei auf Humanpräparate angewiesen zu sein.

L’hypertension pulmonaire (HTP) est la principale pathologie de la circulation pulmonaire et a un très mauvais pronostic. Elle se caractérise par une hyperréactivité et un remodelage des petites artères pulmonaires (AP) entraînant une augmentation progressive des résistances vasculaires pulmonaires, qui, ultimement, aboutit à une insuffisance cardiaque droite et au décès du patient. Il est admit que le calcium joue un rôle très important aussi bien dans les mécanismes de remodelage que dans l’hyperréactivité des AP observés dans l’HTP. Dans le présent travail, nous avons étudié l’expression et le rôle d’une famille particulière de canaux calciques, les TRPV, dans les AP de rats contrôles (normoxiques) et souffrant d’hypertension pulmonaire (rats hypoxiques chroniques et traités à la monocrotaline). Nous montrons que (1) les canaux TRPV1, V2 et V4 sont exprimés dans les AP et que cette expression est augmentée au cours de l’HTP ; (2) la stimulation de ces canaux par des agonistes spécifiques induit une augmentation de la concentration calcique intracellulaire dans les cellules musculaires lisses (CML) ; (3) le récepteur à la ryanodine de type 2 (RRy 2) du réticulum sarcoplasmique est impliqué dans la voie de signalisation dépendante de TRPV4 et que son expression est également augmentée au cours de l’HTP ; (4) les canaux TRPV1 et TRPV4 sont impliqués dans la migration des CML, processus fondamental du remodelage ; (5) les contractions induites par l’activation de TRPV2 et TRPV4 dans les AP de rats hypertendus sont significativement diminuées par la streptomycine, un inhibiteur des canaux SAC (stretch activated channels). Ce travail démontre donc l’implication des canaux TRPV à la fois dans l’hyperréactivté et le remodelage des AP. De nouveaux traitements ciblant les canaux TRPV pourraient constituer une approche thérapeutique innovante de l’hypertension pulmonaire. / Pulmonary hypertension (PH)) is the primary pathology of the pulmonary circulation and has a very bad prognostic. This disease is characterized by a hyperreactivity and remodelling of small pulmonary arteries (PA) leading to a progressive increase in pulmonary vascular resistance which ultimately leads to right heart failure and death of the patient. It is admitted that calcium plays an important role both in the mechanisms of remodelling and in the hyperresponsiveness of PA observed in PH. In the present work, we studied the expression and the role of a particular family of calcium channels, TRPV channels, in PA from control rats (normoxic) and pulmonary hypertensive rats (chronically hypoxic and monocrotaline-treated rats). We show that (1) TRPV1, V2 and V4 channels are expressed in the PA and that their expression are increased in PH; (2) stimulation of these channels by specific agonists induces an increase in the intracellular calcium concentration in smooth muscle cells (SMC), (3) the ryanodine receptor type 2 (RRy2) of the sarcoplasmic reticulum is involved in the TRPV4-dependent signaling pathway and its expression is also increased in PH, (4) TRPV1 and TRPV4 channels are involved in the migration of SMC, the fundamental process of remodelling, (5) contractions induced by activation of TRPV2 and TRPV4 in the PA from hypertensive rats are significantly decreased by streptomycine, an inhibitor of stretch activated channels (SAC). This work thus demonstrates the involvement of TRPV channels in both the hyperreactivity and remodelling of PA. New treatments targeting TRPV channels could be an innovative therapeutic approach for pulmonary hypertension.

Melanome zählen zu den zehn häufigsten Tumorentitäten weltweit. Bei frühzeitiger Diagnose ist eine Exzision im Gesunden kurativ. Sobald eine Resektion im Gesunden jedoch nicht mehr möglich ist, sinken die Heilungschancen drastisch. Maligne Melanome sprechen wenig auf konventionelle Tumortherapien wie Radiatio und zytostatische Chemotherapie an. Daher werden neue Therapieoptionen in der Melanomtherapie getestet. Neueste Ansätze beziehen sich auf die Modulation von Immunzellen mittels monoklonaler Antikörper sowie die Modifikation der Signaltransduktion über die Mitogen-aktivierte Protein Kinase Kinase (MAPKK = MEK), BRAF und c-KIT. Auch Ionenkanäle stellen eine vielversprechende, zukünftige Option in der Behandlung maligner Melanome dar. Ich konnte zeigen, dass neben der bereits beschriebenen funktionellen Expression des „transient receptor potential“ Kanals TRPM8 in Melanomzelllinien auch TRPA1 in verschiedenen Melanomzelllinien exprimiert und funktionell ist. Die Phytopharmaka Senföl (Allylisothiozyanat; AITC) und Zimtaldehyd zeigen in Melanom-Modellen antitumoröse Effekte. Zudem sind beide Substanzen potente Stimulatoren von TRPA1. In dieser Arbeit wurde untersucht, ob AITC und Zimtaldehyd TRPA1-vermittelt die Proliferation, Apoptose und Migration von Melanomzellen beeinflussen. Das Vorkommen von TRPA1 in verschiedenen Melanomzelllinien wurde auf molekularbiologischer Ebene, mit fluorometrischen Bestimmungen des TRPA1-vermittelten Ca2+-Einstroms sowie in elektrophysiologischen Messungen nachgewiesen. Anschließend wurde die funktionelle Relevanz von TRPA1 bezüglich tumorhemmender Eigenschaften geprüft. Durch die Anwendung von TRPA1-Blockern konnte die AITC- und Zimtaldehyd-induzierte Verminderung der Proliferation nicht aufgehoben werden. Auch bezüglich der Migration und Apoptose konnte keine Korrelation zu einer TRPA1-Modulation festgestellt werden. Daher scheinen die durch AITC und Zimtaldehyd induzierten Effekte höchstwahrscheinlich nicht durch TRPA1 vermittelt zu werden.:1 Bibliographische Beschreibung 4 2 Abkürzungsverzeichnis 6 3 Einführung 8 3.1 Die Superfamilie der TRP-Kanäle und ihre Expression in malignen Tumoren 8 3.2 Weitere Ionenkanalentitäten im malignen Melanom 10 3.3 Klassifikation und Therapie maligner Melanome 11 3.4 Naturstoffe mit TRP-Kanal-aktivierenden Eigenschaften in der Therapie maligner Tumore 13 3.5 TRPA1 - ein Ionenkanal mit chemosensorischen Eigenschaften 14 4 Ableitung der Fragestellung 17 5 Publikation 18 6 Zusammenfassung der Arbeit 28 7 Literaturverzeichnis 31 8 Anlagen 36 8.1 Erklärung über die eigenständige Abfassung der Arbeit 36 8.2 Curriculum Vitae 37 8.3 Publikationen 39 8.4 Danksagung 40

The goal of this thesis is to study the transient behaviour of the SLOWPOKE-2 reactor using Monte-Carlo simulations with the G4-STORK code. G4-STORK is a 3-dimensional Monte-Carlo code derived from the GEANT4 physics simulation toolkit. Methods were developed for the proper treatment of delayed neutrons and a lumped capacitance model was used to track the time-dependent fuel properties (temperature, density) based on the fission power. By validating the methods in G4-STORK with experimental measurements we hope to extend our understanding of reactor transients as well as further develop our methods to model the transients of the next generation reactor designs. A SLOWPOKE-2 reactor such as the one at RMC was chosen for simulation due to its compact size, and well-known transient response of control rod removal and measured temperature feedback. Static simulations in G4-STORK find a neutron flux of order 10^12 cm−2 s−1 which agrees with experiment and a control rod worth of (4.9 ± 2.0) mk compared to the experimentally measured worth of 5.45 mk. Transient simulations from rod pluck-out find similar trends to the experimental findings as our results suggest a negative temperature feedback due to the doppler broadening of the U-238 absorption spectrum which contributes to the overall safety mechanism seen in the SLOWPOKE reactor. It is determined that the methods in G4-STORK provide a reasonable ability to simulate reactor transients and it is recommended that a full-core thermal-hydraulics model be coupled to G4-STORK to achieve a higher level of accuracy. / Thesis / Master of Applied Science (MASc)

The company Kanthal produces electric heating elements that require high temperature treatment in one production step. In this process step, called sintering, the amount of heat received by the sintered material is in direct correlation to the product’s outcome.  It is therefore of interest for the company to gather information about how heat transfer happens in an electrical furnace. This study examines two different possible scenarios of how the heat transfer in the furnace could look like and which amount of heat the sintered material would receive. The relation between a gaseous ambience at a certain temperature and the temperature an object submerged into this ambience is assuming is studied in the process called "transient heat conduction".  Two models were built in Matlab, representing transient heat conduction effects on two different geometries: a plane wall and a short cylinder.  It could be shown that transient heat conduction effects turned out differently for the two models. The conclusion drawn from the results was that the wall model was susceptible to horizontal heat transfer effects, whereas the cylinder model was affected from all directions equally. Further, an analysis of the heat transfer channels within the furnace revealed that the heat leakage through the furnace muffle edges, which are in contact with air, causes a multiple in heat loss compared to the overall heat leakage.