The aromatic amino acids tryptophan, tyrosine, and phenylalanine have been extensively used for different label-free protein studies. These investigations extract information on protein conformations and interactions from the emitted fluorescence's intensity, wavelength, and/or polarization. Like most fluorescent organic compounds, these amino acids also undergo transitions into dark meta-stable states, including triplet and photo-radical states. These transitions are notably sensitive to the surrounding environment, offering an additional set of parameters that reflect the protein's interactions, folding states, and immediate surroundings. Transient State (TRAST) monitoring has been developed to quantify fluorophore transition dynamics by recording the average fluorescence intensity in response to a modulated excitation. In this work, we performed TRAST experiments to investigate tyrosine autofluorescence and used it to detect conformational changes in calmodulin, a calcium-binding protein containing two tyrosine residues. A photophysical model for tyrosine was established, and it was revealed how tyrosine's dark state transitions changed with excitation intensity, solvent pH, and redox conditions. The TRAST experiments demonstrated that tyrosine's dark state transitions could serve as valuable information sources for label-free analyses of protein conformations and interactions. / De aromatiska aminosyrorna tryptofan, tyrosin och fenylalanin har använts i stor utsträckning för olika inmärkningsfria proteinstudier. Dessa undersökningar extraherar information om proteinkonformationer och interaktioner från den emitterade fluorescens intensiteten, dess våglängd och/eller polarisering. Liksom de flesta fluorescerande organiska föreningar genomgår dessa aminosyror också övergångar till mörka metastabila tillstånd, inklusive triplett- och fotoradikaltillstånd. Dessa övergångar är särskilt känsliga för den omgivande miljön, och erbjuder en extra uppsättning parametrar som återspeglar proteinets interaktioner, vikningstillstånd och omedelbara omgivningar. Transient State (TRAST) monitorering har utvecklats för att kvantifiera fluoroforövergångsdynamik genom att registrera den genomsnittliga fluorescensintensiteten som svar på en modulerad excitation. I detta arbete utförde vi TRAST-experiment för att undersöka tyrosinautofluorescens och använde den för att detektera konformationsförändringar i calmodulin, ett kalciumbindande protein som innehåller två tyrosiner. En fotofysikalisk modell för tyrosin etablerades, och hur tyrosins mörka tillståndsövergångar förändrades med excitationsintensitet, lösningsmedels pH och redoxförhållanden kunde faststållas. TRAST- experimenten visade att tyrosins mörka tillståndsövergångar kan fungera som värdefulla informationskällor för inmärkningsfria analyser av proteinkonformationer och interaktioner.

Engine mapping is the process of empirically modelling engine behaviour as a function of adjustable engine parameters, predicting the output of the engine. The aim is to calibrate the electronic engine controller to meet decreasing emission requirements and increasing fuel economy demands. Modern engines have an increasing number of control parameters that are having a dramatic impact on time and e ort required to obtain optimal engine calibrations. These are further complicated due to transient engine operating mode. A new model-based transient calibration method has been built on the application of hierarchical statistical modelling methods, and analysis of repeated experiments for the application of engine mapping. The methodology is based on two-stage regression approach, which organise the engine data for the mapping process in sweeps. The introduction of time-dependent covariates in the hierarchy of the modelling led to the development of a new approach for the problem of transient engine calibration. This new approach for transient engine modelling is analysed using a small designed data set for a throttle body inferred air ow phenomenon. The data collection for the model was performed on a transient engine test bed as a part of this work, with sophisticated software and hardware installed on it. Models and their associated experimental design protocols have been identi ed that permits the models capable of accurately predicting the desired response features over the whole region of operability. Further, during the course of the work, the utility of multi-layer perceptron (MLP) neural network based model for the multi-covariate case has been demonstrated. The MLP neural network performs slightly better than the radial basis function (RBF) model. The basis of this comparison is made on assessing relevant model selection criteria, as well as internal and external validation ts. Finally, the general ability of the model was demonstrated through the implementation of this methodology for use in the calibration process, for populating the electronic engine control module lookup tables.

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My master´s thesis named “Automated measurement of transient phenomena of synchronous generators“ deals with transient phenomena appearing during a three-phase short-circuit on output clips of synchronous generators. Introduction of my master´s thesis named “Theory of transient phenomena of synchronous generators” deals with a theoretic description of transient phenomena appearing during a three-phase short-circuit on output clips of synchronous generators. This part treats the existence of direct and alternating components of short-circuit currents flowing in stator winding during a three-phase short circuit. Afterwards it treats reactances of stator windidng for the duration of a three-phase short-circuit on clips of synchronous generators. Theme of the following part of the master´s thesis is “Technical setting of measuring system for analysis of transient phenomena of synchronous generators”. This part deals with an analysis of technical setting that characterizes measuring sensors. These sensors scan measured electrical magnitudes that describe transient phenomena. Measuring hardware by a firm National Instruments is designed on the basis of technical setting. Following part of the master´s thesis named “Measuring hardware for measuring of transient phenomena of synchronous generators as a platform PXI/SCXI” deals with a description of technical characteristics of designed measuring hardware for measuring transient phenomena. Practical part of the master´s thesis named “Software TransientVIEW for analysis of transient phenomena of synchronous generators” describes in detail a software conception of user interface of software TransientVIEW that is developed in a developing interface LabVIEW 8.6 by the firm National Instruments. Afterwards it treats implemented user functions that allows exact and effective work with analysis of transient phenomena of synchronous generators.

Trafikverket operates about 12000 Switches and crossings (SnCs) of which 6800 are equipped with an electrical heating system of 10−30kW power to keep SnCs functional throughout the winters by keeping them free of Snow/Ice. The energy consumption is approximately 200−130GWh/year costing approximately 10−15 million Euros annually. Electricity demand is continuously rising in Sweden and if coupled with unpredictable events can highly impact the grid energy mix and electricity prices which can lead to a higher operating carbon footprint & expenses. This master thesis work is developed through a couple of research programs to investigate the power usage in wayside objects. Currently, work is in progress to make the wayside objects ‘smart’ so that they can operate fully autonomously with two objectives to reduce the overall power consumption and obtain it from local energy resources. Mathematical modeling of unsteady close contact melting of rectangular Ice/Snow blocks of different volumes on the horizontal surfaces has been solved using numerical methods to calculate the melting time and power requirement. A parametric study has been done for various sizes, and initial & hot surface temperatures. Further, using ANSYS Fluent, CFD simulations have been performed to calculate the heat transfer rate from the Rail body for various combinations of heat source & ambient temperature, and wind speeds. These results shall help to devise a control strategy for the dynamic power supply which can help to optimize the power consumption. Finally, evaluating the renewable resources potential; ground source heat pumps using borehole U-pipes appear to be the best option which can drastically reduce the electricity requirement for the optimized heating power requirement. Most of Sweden has granite bedrock which is one of the reasons for the successful deployment of heat pumps. It’s definitely worth expanding its usage in other sectors as well like Railways. / Trafikverket driver cirka 12 000 spårväxlar och spårkorsningar varav 6 800 är utrustade med ett elektriskt värmesystem på 10 - 30kW effekt för att hålla växlar och korsningar funktionella under hela vintrarna genom att hålla dem fria från snö/is. Energiförbrukningen är cirka 200-130 GW h/år och kostar cirka 10-15 miljoner euro per år. Efterfrågan på el ökar kontinuerligt i Sverige och kan i kombination med oförutsägbara händelser i hög grad påverka nätets energimix och elpris vilket i sin tur kan leda till ett högre koldioxidavtryck och högre driftskostnader. Detta examensarbete utvecklades genom ett par forskningsprogram med fokus på strömförbrukningen i järnvägssystemet. Just nu pågår ett arbete med att göra järnvägssystemet "smart" så att det kan fungera helt automatiskt med två mål: att minska den totala energiförbrukningen och få den från lokala energiresurser. Kontaktsmältning av rektangulärt is/snö-block av olika volymer på horisontella ytor har lösts med hjälp av numeriska metoder för att beräkna smälttid och effektbehov. En parametrisk studie har gjorts för olika storlekar och initiala och varma yttemperaturer. Vidare, med hjälp av ANSYS Fluent, har CFD-simuleringar utförts för att beräkna värmeöverföringshastighet från järnvägsspåret för olika kombinationer av värmekälla och omgivning temperatur och vindhastigheter. Dessa resultat ska bidra till att utforma en styrstrategi för den dynamiska strömförsörjningen som kan bidra till att optimera strömförbrukningen. Slutligen, för att utvärdera potentialen för förnybara resurser, verkar bergvärmepumpar som använder borrhåls U-rör vara det bästa alternativet som drastiskt kan minska elbehovet för det optimerade värmeeffektbehovet. Större delen av Sverige har berggrund av granit vilket är en av anledningarna till den framgångsrika utbyggnaden av värmepumpar. Det är definitivt värt att utöka användningen i andra sektorer också som järnvägar.

Esta dissertação aborda o cálculo da taxa de crescimento da tensão de restabelecimento transitória (TCTRT), considerando o caso de falha na barra, durante a abertura do primeiro polo e o cálculo da tensão de restabelecimento transitória (TRT) nos primeiros instantes de tempo, incluindo o efeito da capacitância. Os principais casos possíveis de falha na barra foram analisados e os resultados demonstraram a precisão das expressões obtidas. Normalmente, o cálculo da TCTRT e da TRT é feito por meio de simulações em programas de transitórios eletromagnéticos em que há rotinas específicas para a extração dos valores destas, porém, em determinadas condições, podem ocorrer oscilações ou imprecisões numéricas. Por ser um assunto de grande interesse convém desenvolver expressões que possam esclarecer resultados duvidosos sem a necessidade de artifícios de redução do passo de integração a valores muitas vezes não factíveis. As expressões obtidas neste trabalho podem ser utilizadas não só para se obter de forma precisa e simplificada os valores da taxa de crescimento da tensão de restabelecimento transitória de um circuito real, assim como os valores da TRT nos primeiros instantes de tempo. / This study is focused on the rate of rise of transient recovery voltage (RRTRV) calculation considering the case of bus fault, during the first pole to open, and also includes the transient recovery voltage (TRV) calculation, in the first instants of time, including the capacitance effect. The main cases of bus fault have been evaluated and demonstrate that the expressions developed in this study are relevant and with good precision. Usually, the RRTRV and the TRV are calculated with the use of simulation programs, using specific routines, nevertheless, in certain conditions, there might be oscillations and numeric imprecisions which requires a mathematic expression. This topic is of great interest and it is important to have mathematical expressions that could clarify doubtful results. The expressions obtained in this study can be use in a very simples and effective mode to calculate RRTRV and the TRV in the first instants of time, considering a real life circuit.

Esta dissertação aborda o cálculo da taxa de crescimento da tensão de restabelecimento transitória (TCTRT), considerando o caso de falha na barra, durante a abertura do primeiro polo e o cálculo da tensão de restabelecimento transitória (TRT) nos primeiros instantes de tempo, incluindo o efeito da capacitância. Os principais casos possíveis de falha na barra foram analisados e os resultados demonstraram a precisão das expressões obtidas. Normalmente, o cálculo da TCTRT e da TRT é feito por meio de simulações em programas de transitórios eletromagnéticos em que há rotinas específicas para a extração dos valores destas, porém, em determinadas condições, podem ocorrer oscilações ou imprecisões numéricas. Por ser um assunto de grande interesse convém desenvolver expressões que possam esclarecer resultados duvidosos sem a necessidade de artifícios de redução do passo de integração a valores muitas vezes não factíveis. As expressões obtidas neste trabalho podem ser utilizadas não só para se obter de forma precisa e simplificada os valores da taxa de crescimento da tensão de restabelecimento transitória de um circuito real, assim como os valores da TRT nos primeiros instantes de tempo. / This study is focused on the rate of rise of transient recovery voltage (RRTRV) calculation considering the case of bus fault, during the first pole to open, and also includes the transient recovery voltage (TRV) calculation, in the first instants of time, including the capacitance effect. The main cases of bus fault have been evaluated and demonstrate that the expressions developed in this study are relevant and with good precision. Usually, the RRTRV and the TRV are calculated with the use of simulation programs, using specific routines, nevertheless, in certain conditions, there might be oscillations and numeric imprecisions which requires a mathematic expression. This topic is of great interest and it is important to have mathematical expressions that could clarify doubtful results. The expressions obtained in this study can be use in a very simples and effective mode to calculate RRTRV and the TRV in the first instants of time, considering a real life circuit.

<p>Electromagnetic transients impinging on electromagnetic power devices - such as electric machines, transformers and reactors - can stress the design severely. Thus the magnitudes of the transients are often decisive for the design of the devices. Further, the operation of a device can be transient in itself. This is the case for the explosive magnetic flux compression generator (EMG) and a ferromagnetic actuator. </p><p>Models are presented that are mainly intended for transients in the millisecond range and faster. Hence, eddy currents and the related skin and proximity effect become significant in windings, magnetic cores and in the armatures of the devices. These effects are important for, e.g., the damping of the transients. Further, the displacement current in the insulation of the winding is significant. It changes the response of the windings dramatically, as it manifests the finite velocity of propagation of the electromagnetic fields. Under such circumstances, reflections and excited resonances can make the transient voltage and current distribution highly irregular. </p><p>Induced voltages are modelled with self and mutual inductances or reluctances combined with winding templates. The displacement currents are modelled with capacitances or coefficients of potential. Cauer circuits and their dual form are used to model eddy currents in laminated cores and in conductors. The Cauer circuit enables one to consider hysteresis and the non-linear response of a magnetic core. It is also used to model the eddy currents in the moving armature of an EMG. </p><p>A set-up is presented that can be used to study the transient voltage and the current distribution along a coil. </p><p>The transient response of coaxially insulated windings is analysed and modelled in detail. A lumped circuit model is developed for a coil, Dryformer^TM - the new high-voltage transformer - and Powerformer^TM, the new high-voltage generator. An alternative model, a combined lumped circuit and FEM model, is presented for a coaxially insulated winding in two slot cores.</p>

Electromagnetic transients impinging on electromagnetic power devices - such as electric machines, transformers and reactors - can stress the design severely. Thus the magnitudes of the transients are often decisive for the design of the devices. Further, the operation of a device can be transient in itself. This is the case for the explosive magnetic flux compression generator (EMG) and a ferromagnetic actuator. Models are presented that are mainly intended for transients in the millisecond range and faster. Hence, eddy currents and the related skin and proximity effect become significant in windings, magnetic cores and in the armatures of the devices. These effects are important for, e.g., the damping of the transients. Further, the displacement current in the insulation of the winding is significant. It changes the response of the windings dramatically, as it manifests the finite velocity of propagation of the electromagnetic fields. Under such circumstances, reflections and excited resonances can make the transient voltage and current distribution highly irregular. Induced voltages are modelled with self and mutual inductances or reluctances combined with winding templates. The displacement currents are modelled with capacitances or coefficients of potential. Cauer circuits and their dual form are used to model eddy currents in laminated cores and in conductors. The Cauer circuit enables one to consider hysteresis and the non-linear response of a magnetic core. It is also used to model the eddy currents in the moving armature of an EMG. A set-up is presented that can be used to study the transient voltage and the current distribution along a coil. The transient response of coaxially insulated windings is analysed and modelled in detail. A lumped circuit model is developed for a coil, DryformerTM - the new high-voltage transformer - and PowerformerTM, the new high-voltage generator. An alternative model, a combined lumped circuit and FEM model, is presented for a coaxially insulated winding in two slot cores.

The need for energy is growing in the world and the market of nuclear power is now once more expanding. Some issues of the current light-water reactors can be solved by the next generation of nuclear power, Generation IV, where sodium-cooled reactors are one of the candidates. Phénix was a French prototype sodium-cooled reactor, which is seen as a success. Although it did encounter an earlier unexperienced phenomenon, A.U.R.N., in which a negative reactivity transient followed by an oscillating behavior forced an automatic emergency shutdown of the reactor. This phenomenon lead to a lot of downtime of the reactor and is still unsolved. However, the most probable cause of the transients is radial movements of the core, referred to as core-flowering. This study has investigated the available documentation of the A.U.R.N. events. A simplified model of core-flowering was also created in order to simulate how radial expansion affects the reactivity of a sodium-cooled core. Serpent, which is a Monte-Carlo based simulation code, was chosen as calculation tool. Furthermore, a model of the Phénix core was successfully created and partly validated. The model of the core has a k_eff = 1.00298 and a neutron flux of (8.43+-0.02)!10^15 neutrons/cm^2 at normal state. The result obtained from the simulations shows that an expansion of the core radius decreases the reactivity. A linear approximation of the result gave the relation: change in k_eff/core extension = - 60 pcm/mm. This value corresponds remarkably well to the around - 60 pcm/mm that was obtained from the dedicated core-flowering experiments in Phénix made by the CEA. Core-flowering can recreate similar signals to those registered during the A.U.R.N. events, though the absence of trace of core movements in Phénix speaks against this. However, if core-flowering is the sought answer, it can be avoided by design. The equipment that registered the A.U.R.N. events have proved to be insensitive to noise. Though, the high amplitude of the transients and their rapidness have made some researcher believe that the events are a combination of interference in the equipment of Phénix and a mechanical phenomenon. Regardless, the origin of A.U.R.N. seems to be bound to some specific parameter of Phénix due to the fact that the transients only have occurred in this reactor. A safety analysis made by an expert committee, appointed by CEA, showed that the A.U.R.N. events are not a threat to the safety of Phénix. However, the origin of these negative transients has to be found before any construction of a commercial size sodium-cooled fast reactor can begin. Thus, further research is needed.