The best characterized of the autonomous parvoviruses are those of rodent origin. The purpose of this study was to examine the physicochemical characteristics of the genome of an autonomous parvovirus of non-rodent origin, bovine parvovirus (BPV). BPV was isolated from infected cells by centrifugation through a sucrose-CsCl step-gradient. The virion DNA was released from the capsids by alkali treatment. Upon chromatography on hydroxyapatite, two peaks of radioactivity were consistently observed. Peak I eluted at a sodium phosphate concentration of 0.17 M, intermediate between single- and double-stranded DNA markers. Peak II eluted in the same position as double-stranded DNA. The DNA eluting in peak I represented the viral genome. About 7% of the genome length was found to exist as a duplex. The BPV DNA was found to have sedimentation coefficients of 16.5 Sand 27 S in alkaline and neutral sucrose, respectively. These S values corresponded to a molecular weight of approximately 1.7 x 10⁶ daltons. The BPV DNA was rich in thymine (31.3%) and had a GC content of 45.1%. The buoyant density of the single-stranded BPV genome was determined to be 1.721 g/cm³. The DNA eluting in peak II was shown by hybridization to be of BPV origin. Furthermore, the peak II DNA was shown to be of the same size as the single-stranded BPV genome, by sedimentation in alkaline sucrose. Electron microscopy revealed that the peak II DNA was linear and double-stranded. It is concluded that BPV packages the plus strand into separate virions in significant amounts, resulting in the reannealing of complimentary plus and minus strands upon release from the virions during isolation of the DNA / M.S.

Two lines of chickens, derived by two-way selection for either a high (HPC) or low (LPC) plasma corticosterone response to social stress, were inoculated with Marek's disease virus (MDV). Lymphocyte transformation (LT) microassays were performed to assess the CMI response of leucocytes from HPC and LPC MDV-infected chickens. Blastogenesis was quantitated by ³H-thymidine uptake. Leucocytes from chickens specifically sensitized with either heat-killed tuberculin or 1,5-di-fluoro-2,4-dinitrobenzene were subsequently stimulated in vitro with either purified protein derivative (PPD) or dinitrophenyl-bovine serum albumin (DNP-BSA) conjugate. Phytohemagglutinin (PHA-P) was used to assess non-specific lymphocyte transformation. MDV-infected HPC and LPC chicken leucocytes were also compared for their ability to lyse ⁵¹Cr-labeled fowl erythrocytes upon stimulation with PHA-P. Leucocytes from non-infected LPC control chickens underwent blastogenesis to a greater extent than did non-infected HPC control chickens. Although all tumor-bearing chickens demonstrated a markedly suppressed LT response, LPC chickens demonstrated a greater LT response than did HPC chickens. Relative to steroid level chickens infected with MDV, but without tumors, demonstrated a suppressed LT response; however, this response was greater than that of chickens with tumors, but less than that of normal controls. Under conditions of sustained high social stress, HPC chickens demonstrated a decreased cell-mediated cytotoxicity (CMC) in comparison with LPC chickens. In both steroid lines, CMC decreased with the onset and severity of tumors. The impaired CMI seen in the HPC line corresponded with an increased tumor incidence and a greater mortality in this line than in the LPC line. Metyrapone, a chemical which blocks adrenal corticosterone synthesis, transiently enhanced CMC and initiated tumor regression in birds debilitated with MD tumors. Early treatment with 500 ppm metyrapone mixed in feed was most effective. / M.S.

Members of the genus, Treponema, require long-chain fatty acids for growth in vitro. Serum, added to culture medium, provides a source of long-chain fatty acids. These fatty acids, however, are esterified to triglycerides, phospholipids, and cholesterol. In this study, the major pathways of complex lipid catabolism in five species of Treponema were investigated. Lipase, the enzyme which hydrolyzes triglycerides, was demonstrated using four lipid substrates in five species. Chromatographic data demonstrated that, during growth, treponemes completely utilized lysophosphatidylcholine, present in serum-supplemented culture medium, while phosphatidylcholine and phosphatidylinositol were not utilized. Whole cultures and cell free extracts were examined for the presence of specific enzymes of phospholipid catabolism. Phospholipase B and glycerophosphorylcholine diesterase activities were demonstrated in five species of Treponema. Acid phosphatase activity was demonstrated in two species. Utilization of L-α-glycerophosphate and glycerol, products of phospholipid catabolism, was examined. Glycerol was utilized from culture medium by one species, while α-glycerophosphate was utilized by four species. Phospholipases A, C, and D, α-glycero-phosphate dehydrogenase, and alkaline phosphatase activities were not found in five species of Treponema. Based on the specific enzymes demonstrated in this study, two pathways of phospholipid catabolism are proposed. / M.S.

李家祥. / 手稿本(cops. 2 & 3 複印本) / Thesis (M.A.)--香港中文大學硏究院哲學學部. / Shou gao ben (cops. 2 & 3 fu yin ben) / Includes bibliographical references: leaves 170. / Li Jiaxiang. / Thesis (M.A.)--Xianggang Zhong wen da xue yan jiu yuan zhe xue xue bu. / Chapter 第一章 --- 前言 --- p.1 / Chapter a --- 序 --- p.1 / Chapter b --- 關於唐君毅先生著作的分歧問題 --- p.7 / Chapter c --- 唐君毅先生早年形上學要略 --- p.14 / Chapter 第二章 --- 証立心之本體之道路 --- p.20 / Chapter a --- 世間悲情之現起 --- p.23 / Chapter b --- 超越要求之出現 --- p.28 / Chapter c --- 從用見體 --- p.37 / Chapter d --- 本體之証立 --- p.49 / Chapter 第三章 --- 心之本體的特種特性 --- p.59 / Chapter a --- 本體之在其自身 --- p.59 / Chapter b --- 本體之表現 --- p.63 / Chapter c --- 本體之無限性 --- p.68 / Chapter d --- 本體之至善 --- p.74 / Chapter 第四章 --- 現實世界之存有上之形上根據 --- p.78 / Chapter a --- 世界之顯現 --- p.79 / Chapter b --- 物質之存有性 --- p.86 / Chapter 第五章 --- 人之存有性 --- p.96 / Chapter a --- 「人」是什麼? --- p.98 / Chapter 第六章 --- 心之本體之自我回歸 --- p.106 / Chapter a --- 罪惡 --- p.112 / Chapter b --- 道德 --- p.115 / Chapter c --- 本體回歸之途程 --- p.119 / Chapter d --- 餘論 --- p.135 / Chapter 第七章 --- 評論 --- p.140 / Chapter a --- 唐先生對哲學的態度 --- p.141 / Chapter b --- 對唐先生哲學相干的批評 --- p.144 / Chapter c --- 唐先生早年形上學所提供的世界觀對人生活動之指向 --- p.145 / Chapter d --- 契入這個世界觀的道路 --- p.151 / Chapter e --- 唐先生晚年對這一型形上學之安頓 --- p.156 / Chapter f --- 總結 --- p.168 / 參考書目 --- p.170

Th. 3e cycle--Géogr.--Saint-Étienne, 1975.

No description available.

No description available.

Univ., Diss.--Trier, 2003.

by Victoria Y. Jin, Alexander H. Levis. / Cover title. / Includes bibliographical references (p. 9). / Research supported by the Office of Naval Research. N00014-84-K-0519 Research supported by the Basic Research Group of the Joint Directors of Laboratory through the Office of Naval Research. N00014-85-K-0782

Th. 3e cycle--Sci. écon.--Rennes 1, 1979.