People dying of HIV/AIDS and the delay in finding a cure has put education programmes at the centre of HIV/AIDS management. The study purpose is to investigate the effects of Life Orientation programme on HIV/AIDS prevention knowledge, attitudes and behaviours amongst college students at a TVET College in Randburg. A quantitative descriptive cross-sectional design was used, and a questionnaire was administered to level 1 students (n=141). Data was collected, and analysed using SPSS version 25. The findings showed a relationship between Life Orientation (LO) and knowledge, attitudes and behaviour of students towards HIV/AIDS. Students with high Life Orientation perception reported adequate knowledge and positive attitudes. Many respondents revealed high levels of HIV/AIDS knowledge, healthy behaviour and positive attitude towards HIV/AIDS, showing that Life Orientation had aided in those areas. However, some students had poor perceptions of HIV/AIDS knowledge, henceforth lacked the confidence to protect themselves. Several recommendations to health promoters, education policymakers and the community were given. / Health Studies / M. P. H.