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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
41

Neutron tunneling in nanostructured systems: isotopical effect

Matiwane, Aphiwe 11 1900 (has links)
Tunneling phenomenon has been studied since the time of Sir Isaac Newton. In the case of neutron tunneling phenomenon, it is the quantum mechanics wave-particle duality which manifests itself. In this case, particularly, the neutron wave-packet under total reflection condition suffers the so-called frustrated total reflection as known in standard optics. More accurately, this tunneling phenomenon shows itself via sharp dips in the plateau of total reflection. The prerequisite to observe such quantum mechanics phenomenon lies within a thin film Fabry-Perot resonator configuration. This thin film Fabry-Perot resonator geometry consists of two reflecting mirrors separated by a transparent material from a neutron optics viewpoint. In view of the specific neutron scattering properties related to the spin of the neutron wave-packet. As a direct proof, isotopic nickel based thin films Fabry-Perot resonator have been fabricated by depositing thin film of nickel by ion beam sputtering. The vacuum chamber was pumped down to the pressure of 10-8 mbar and deposition was performed at pressure of 2x10-4 mbar. The deposition rate was kept at 1.5 nm / minute and thickness layers were monitored by a calibrated quartz microbalance. Unpolarized neutron reflectometry measurements were carried out at the ORPHEE reactor using the time-of-flight EROS reflectometer. The incidence neutron wavelength varied between 3 – 25 Å. The grazing angle and angular resolution were of the order of 0.8˚ and 0.05 respectively. The software program, a Matlab routine for the simulation of specular X-ray and neutron reflectivity data with matrix technique, was employed to simulate the phenomenon and thereafter the experimentally obtained data and calculated (theoretical) data were compared. From the analysis of the comparison, a conclusion was drawn about the agreement between experimental data and theoretical data. The tunneling phenomenon has been observed in nanostructured isotopic nickel based thin film Fabry-Perot resonator. It manifested itself by the existence of dips, tunneling resonances, in the total reflection plateau due to quasi-bound states in the isotopic nickel based thin film Fabry-Perot resonator. In total, there were 7 tunneling resonances. The full widths at half maximum of these dips were found to decrease with increasing momentum wave vector transfer (Q) and this correlated to the neutron lifetime in the nanostructured isotopic nickel based thin film Fabry-Perot resonator. / Physics / M. Sc. (Physics)
42

Système microfluidique à onde élastique de surface : vers la duplication d'ADN par PCR / Microfluidic system using surface acoustic wave : Toward the DNA amplification by PCR

Roux-Marchand, Thibaut 02 December 2013 (has links)
Un système microfluidique à onde élastique de surface a été développé dans le but de réaliser une réaction d'amplification de brins d'ADN par PCR. Nous avons principalement étudié la température et l'uniformité de l'échauffement des gouttes irradiées par des ondes de type Rayleigh. Ces dernières sont générées à la surface d'un substrat piézoélectrique de Niobate de Lithium (LiNbO3). Nous avons pensé un système consommant le moins d'énergie électrique possible pour atteindre les températures désirées et permettant une meilleure uniformité de la température des gouttes. Pour cela, un dispositif à transducteur enterré a été réalisé sous une couche isolante de silice. Les gouttes sont ainsi directement posées sur le transducteur ce qui limite les pertes et améliore la répartition de la chaleur au sein des gouttes. Nous avons ensuite vérifié que les réactifs de la PCR ne sont pas affectés par les ondes de Rayleigh ce qui laisse présager que la PCR peut être réalisée à l'aide d'un système d'échauffement par ondes de Rayleigh. Par ailleurs, le déplacement de goutte sur ce type de substrat de LiNbO3 est important pour des applications de type laboratoire sur puce. Ce substrat ayant des propriétés hydrophiles, des revêtements ont été développés afin de minimiser la force nécessaire à l'actionnement des gouttes. Dans ces travaux, nous proposons un nouveau type de couche à base de copolymère P(VDF-TrFe) dont la fabrication est simplement réalisée par dissolution et étalement par spin-coating. Nous avons montré que ce type de couche n'affecte que très peu la propagation des ondes de Rayleigh et les propriétés hydrophobes sont équivalentes à d'autres revêtements / In this work, a microfluidic system based on surface acoustic wave has been developed in order to achieve the amplification of DNA strands by temperature cycling (PCR). We studied mainly the temperature and the heat uniformity of microdroplets irradiated by Rayleigh waves. These waves are generated at the surface of a lithium niobate substrate. We propose a system allowing better temperature uniformity within microdroplets with an optimal energy consumption. For this, a device with buried transducer has been developed under an insulating layer (Silice). The droplets are then placed directly on the transducer which limits losses and improves the distribution of heat within the microdroplets. We then verified that the PCR reagents are not affected by the Rayleigh waves which suggests that PCR can be performed using a heating system by Rayleigh waves. Moreover, the move of microdroplets on this kind of LiNbO3 substrate is important for lab on chip applications. This substrate having hydrophilic properties, some coatings have been developed to minimize the required force to actuate the microdroplets. In this work, we developed a new hydrophobic layer based on copolymer P(VDF-TrFe) whose production is simply made by dissolving and spreading by spin-coating. We have shown that this kind of layer is compatible with Rayleigh waves and that the hydrophobic properties are equivalent to other coatings

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