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Alpha-tocopherol in the treatment of tardive dyskinesiaShriqui, Christian L. January 1990 (has links)
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Studies of tocopherol and phospholipid metabolism in plantsHardy, D. J. January 1988 (has links)
No description available.
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Validation Of QTL Associated With Tocopherol Levels In Three Half-Sib Populations Derived From Keszthelyi Aproszemu Sarga SoybeanMacDuff, Mark 10 January 2012 (has links)
Alpha (α-) tocopherol is the most active component of vitamin E and is a highly desirable and heritable trait in soybean seed. The objectives of this study were to identify and validate regions of the soybean genome that correspond to tocopherol accumulation in seed. A mapping population (07-225) of 190 RILs was derived from a cross between Keszthelyi A.S. x 2355RR which identified that α-tocopherol expression in soybean seed was strongly associated with the Satt381 – Satt196 region of chromosome 9 (LG K) in lines with Keszthelyi A.S. in their pedigree. Two other F4:5 validation populations derived from the crosses Keszthelyi A.S. x PRO 2995R (population 07-223; 94 RILs) and Keszthelyi A.S. x 2355RR (population 07-226; 45 RILs) were used to validate QTL in literature. The markers Satt079 (Ch 6; LG C2), Satt440 (Ch 20; LG I) and Satt381 (Ch 9; LG K) were significantly associated with α-tocopherol expression in these populations. / OMAFRA Food Program, Grain Farmers of Ontario, CanAdvance, Hendrick Seeds
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Phytol aus dem Chlorophyllabbau ist limitierend für die Tocopherol (Vitamin E)-Synthese / Phytol from chlorophyll degradation is limiting for tocopherol (vitamin E)-synthesisKanwischer, Marion January 2007 (has links)
Phytol aus dem Chlorophyllabbau ist limitierend für die Tocopherol (Vitamin E)-Synthese
Als Bestandteil von Chlorophyll ist Phytol das am häufigsten vorkommende Isoprenoid in der Biosphäre. Große Mengen an Chlorophyll werden jährlich degradiert und dabei wird Phytol freigesetzt, über dessen Verbleib jedoch wenig bekannt ist. Es sollte der Nachweis erbracht werden, dass im Zuge des Chlorophyllabbaus hydrolysiertes Phytol Eingang in die Synthese anderer Phytylderivate findet.
Während der Gehalt an Tocopherol, Chlorophyll und Fettsäurephytylester entwicklungs- bzw. seneszenzabhängig ist, bleibt der Gehalt an Phyllochinon etwa gleich. Auch in Samen ist der Gehalt von Tocopherol, Chlorophyll und Fettsäurephytylester entwicklungsabhängig. Es wurde gefolgert, dass nur die Synthesen von Tocopherol und Fettsäurephytylester während des Chlorophyllabbaus stimuliert werden. Daher sollten Mutanten analysiert werden, welche im Chlorophyllabbau inhibiert sind. Da Chlorophyllase den ersten Schritt des Chlorophyllabbaus katalysiert, wurden zwei unabhängige T-DNA-Insertionsmutanten für Chlorophyllase1 (CHL1) und eine T-DNA-Insertionsmutante für Chlorophyllase2 (CHL2) identifiziert und eine chl1-1chl2-Doppelmutante erzeugt. Die Analyse der Chlorophyllidanteile ergab eine im Vergleich zum Wildtyp starke Reduktion in den chl1-Mutanten, während der Chlorophyllidanteil von chl2 ähnlich hoch dem Wildtyp ist. Der Chlorophyllidanteil sich entwickelnder chl1-1chl2-Pflanzen nahm in der Seneszenz zu. Die Chlorophyllasemutanten zeigten kein verändertes Seneszenzverhalten im Vergleich zu den Wildtypen. Ferner konnte in den chl1-Linien nur geringfügig weniger Tocopherol und Fettsäurephytylester als in den Wildtypen nachgewiesen werden. Auch der Tocopherolgehalt der Samen war in den Chlorophyllasemutanten unverändert zu den Wildtypen. Aufgrund dessen wurde gefolgert, dass neben den Chorophyllasen CHL1 und CHL2 weitere Chlorophyllhydrolasen in Samen und Blättern von Arabidopsis existieren.
Daher wurde auf andere Mutanten zurückgegriffen, in denen der Chlorophyllabbau stark inhibiert ist und die Seneszenz nach Dunkelinkubation im Vergleich zum Wildtyp deutlich verzögert ist. Eine deutliche Korrelation zwischen vermindertem Chlorophyllabbau und Gehalt an Tocopherol und Fettsäurephytylester konnte in den staygreen-Mutanten pao1 und zwei unabhängigen SGR (staygreen)-RNAi-Linien nachgewiesen werden. Damit konnte eindeutig gezeigt werden, dass die Synthese von Tocopherol und der Fettsäurephytylester durch die Chlorophyllhydrolyse induziert wird. Es wurde gefolgert, dass vor allem unter Seneszenz- bzw. Stressbedingungen dieser alternative Syntheseweg von Phytol eine Rolle spielt. Dennoch kommt der Phytylsynthese durch die de novo-Isoprenoidsynthese auch eine Bedeutung zu. Nach Behandlung von stickstoffmangelgestressten Wildtyppflanzen mit dem Inhibitor Fosmidomycin, welcher die plastidäre de novo-Isoprenoidsynthese hemmt, war der Tocopherolgehalt gegenüber stickstoffmangelgestressten Kontrollpflanzen stark reduziert.
Ferner konnte eine T-DNA-Insertionsmutante der Geranylgeranylreduktase (GGR) identifiziert werden. Diese Mutante kann nur auf Nährmedium überleben, hat nur wenige grüne Blätter und bildet keine Samen. Es konnte kein Phyllochinon, Chlorophyll und keine Fettsäurephytylester, jedoch geringe Mengen Tocopherol nachgewiesen werden. Der Resttocopherolgehalt wird auf die Nebenaktivität einer anderen Reduktase zurückgeführt. Weiterhin wurde nur das Geranylgeranylderivat des Chlorophylls identifiziert. Diese Ergebnisse erlauben den Schluss, dass die phytylgruppenübertragenen Enzyme der Tocopherol-, Phyllochinon- und Fettsäurephytylestersynthese eine hohe Substratspezifität für die Phytylgruppe aufweisen. Nach Fütterung von Phytol konnte in ggr Tocopherol und Chlorophyll bestimmt werden. Aufgrund dessen kann gefolgert werden, dass Chlorophyllsynthetase aus Arabidopsis sowohl Geranylgeranyl-, als auch Phytylpyrophosphat als Substrat nutzen kann und damit ein breiteres Substratspektrum aufweist. / Phytol from chlorophyll degradation is limiting for tocopherol (vitamin E)-synthesis
As a part of the chlorophyll molecule phytol belongs to the most abundant isoprenoid of the biosphere. Huge amounts of chlorophyll are degraded annually. During this process phytol is released, but only little is known about the fate of phytol. The goal of the project was to provide evidence that during chlorophyll degradation released phytol enters the pathway of the synthesis of further phytyl derivatives.
While the content of tocopherol, chlorophyll and fatty acid phytyl esters are growth and stress related the content of phylloquinone does not change during development or under stress conditions. Also in seeds the content of these phytyl derivates are dependent on development. Hence only tocopherol and fatty acid phytyl ester synthesis are induced during chlorophyll degradation. Therefore mutants were analysed that are inhibited in chlorophyll degradation. Chorophyllase catalyses the first step during chlorophyll degradation. Two independent T-DNA insertion mutants of Chlorophyllase1 (CHL1) and one for Chlorophyllase2 (CHL2) were identified. Furthermore chl1-1 and chl2 were crossed to produce the chl1-1chl2 double mutant. The mutation resulted in a strong reduction of the chlorophyllide fraction in chl1 mutants while the chlorophyllide fraction of chl2 was similar to wild type. The chlorophyllide fraction in developing chl1-1chl2 plants increased during senescence. For all chlorophyllase mutants no retardation of senescence was observed. Compared to wild type only marginal reductions in tocopherol and fatty acid phytyl ester contents could be observed for the chl1 mutants. The seed tocopherol content of the chlorophyllase mutants was similar to wild type. Therefore, it was concluded that in leaves and seeds of Arabidopsis besides CHL1 and CHL2 further chlorophyll hydrolases exist that induce chlorophyll degradation.
Thus, staygreen mutants exhibiting strongly inhibited chlorophyll degradation were analysed. Compared to wild type the staygreen mutants pao1 and two independent SGR (staygreen)-RNAi-lines show a strong retardation of senescence under dark incubation. A clear correlation between reduced chlorophyll degradation and tocopherol and fatty acid phytyl ester content could be demonstrated. With this it was possible to verify that tocopherol and fatty acid phytyl ester synthesis are induced by chlorophyll hydrolysis. This alternative pathway seems to play an important role in particular under stress and senescence conditions. Nevertheless, after application of Fosmidomycin, an inhibitor of the plastidic de novo isoprenoid synthesis pathway, to nitrogen starved wild type plants the tocopherol content was strongly reduced compared to nitrogen starved control plants. Therefore, also the plastidic de novo isoprenoid synthesis plays a significant role for tocopherol synthesis.
Moreover, a T-DNA insertion mutant for Geranylgeranyl reductase (ggr) was identified and isolated. This mutant can survive only on nutrition medium, contains only a few green leaves and produces no seeds. There was no phylloquinone, chlorophyll and fatty acid phytyl ester detectable, but minor amounts of tocopherol. The residual amounts of tocopherol were attributed to side activities of another reductase. Obviously, the phytyl transferring enzymes of tocopherol, phylloquinone and fatty acid phytyl ester synthesis exhibit a strong substrate specificity of the phytyl group. After feeding phytol to ggr tocopherol and chlorophyll were detectable in this mutant. Therefore, it was concluded that chlorophyll synthetase from Arabidopsis can use geranylgeranyl pyrophosphate as well as phytyl pyrophosphate as substrates.
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Effects of Select Vitamin E Isoforms on the Production of Polyunsaturated Fatty Acid Metabolites in Colorectal CancerBorketey, Martha A 01 May 2015 (has links)
Vitamin E exhibits anti-tumor activity by regulating pathways in cancer cells, potentially the lipoxygenase (LOX) pathway. We studied the effects of alpha tocopherol (AT), gamma tocopherol (GT), gamma tocotrienol (GT3), and an alpha-gamma tocopherol mixture (ATGT) on the production of the LOX metabolites 13-hydroxyoctadecaenoic acid (HODE), 15-hydroxyeicosatetraenoic acid (HETE), 12-HETE, and 5-HETE in colorectal cancer. These metabolites were examined in the HCT-116 cell line after 24 h treatment with select vitamin E isoforms and quantified by LC/MS/MS. Under physiological conditions, we find that treatment with varying vitamin E isoforms have different effects on the production of 13-HODE, 15-HETE, 12-HETE, and 5-HETE. GT increases 13-HODE and decreases 12-HETE. AT reverses the effects of GT regulation on the LOX pathway, while GT3 has no significant effect on the metabolites tested. GT shows superiority in regulating the LOX pathway as it increases 13-HODE and decreases 12-HETE for possible prevention of colorectal cancer.
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Convenient Preparation of 2,7,8-Trimethyl-6-Hydroxychroman-2-Carboxylic Acid (γ-Trolox)Hyatt, John 01 January 2008 (has links)
The title chroman is useful in synthesis and as a water-soluble analog of γ-tocopherol, a member of the vitamin E family. This new synthesis of γ-trolox proceeds via selective aromatic demethylation of Trolox, the more easily available 2,5,7,8-tetramethyl homolog compound. This route is shorter than the previous synthesis, avoids the use of cyanide and methoxybutadiene, and requires no chromatography.
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Neuroprotection by γ-Tocopherol in Lean and Obese Murine Models ofIschemic StrokeStock, Katie Lauren 24 October 2019 (has links)
No description available.
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Discrimination Between RRR- and All-Racemic-α-Tocopherols Labeled With Deuterium by Patients With AbetalipoproteinemiaTraber, Maret G., Rader, Daniel, Acuff, Robert V., Brewer, H. Bryan, Kayden, Herbert J. 01 January 1994 (has links)
The ability to discriminate between stereoisomers of a-tocopherol was studied in five patients with abetalipoproteinemia (ABL) because an impairment in secretion of apolipoprotein B-containing lipoproteins might impede the normally enhanced plasma transport of RRR-a-tocopherol. An oral dose containing 3.7 g of each 2R,4′R,8′R-α-[5-C2H3]tocopheryl acetate (d3RRR-α-tocopheryl acetate) and 2RS,4′RS,8′RS-α-[5,7-(C2H3),]tocopheryl acetate (d6all rac-α-tocopheryl acetate) was administered, then the labeled and unlabeled a-tocopherol contents of plasma and red blood cells from multiple blood samples obtained at selected times up to 72 h following the dose were quantitated. ABL plasma contained about 1%-10% of the d3-RRR-a-tocopherol concentrations of normal subjects given only 150 mg of each isotope. Three of the patients discriminated between forms of a-tocopherol with ratios of RRR-/allrac-α-tocopherol ≥ 1.8, similar to normals. These data suggest that the hepatic tocopherol binding protein is present and functional in ABL patients. Although two of the patients did not discriminate between stereoisomers of a-tocopherol, it is likely that this resulted from nearly a complete block in very low density lipoprotein (VLDL) secretion. Thus, the ability of ABL patients to absorb and transport orally administered vitamin E is markedly impaired and variable among patients.
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Development of Gamma (γ)-Tocopherol as a Colorectal Cancer Chemopreventive AgentCampbell, Sharon, Stone, William, Whaley, Sarah, Krishnan, Koyamangalath 01 September 2003 (has links)
Nutritional factors play an important role in the prevention and promotion of colorectal cancer. Vitamin E is a generic term that describes a group of lipid-soluble chain-breaking antioxidants that includes tocopherols and tocotrienols. Vitamin E occurs in nature as eight structurally related forms that include four tocopherols and four tocotrienols. Vitamin E is a potent membrane-soluble antioxidant. Antioxidants like vitamin E (tocopherols) may prevent colon cancer through several different cellular and molecular mechanisms. Vitamin E in the American diet is primarily available in plant-oil rich foods such as vegetable oils, seeds and nuts and these foods vary widely in their content of α-tocopherol and γ-tocopherol [1]. Vitamin E may help prevent colon cancer by decreasing the formation of mutagens arising from the oxidation of fecal lipids, by decreasing oxidative stress in the epithelial cells of the colon and by molecular mechanisms that influence cell death, cell cycle and transcriptional events. Most epidemiological, experimental and clinical studies have evaluated the α-isoform and not the γ-isoform of vitamin E. Recent epidemiological, experimental and mechanistic evidence suggests that γ-tocopherol may be a more potent cancer chemopreventive agent than α-tocopherol. The differences in chemical reactivity, metabolism and biological activity may contribute to these differences in the effects of γ-tocopherol when compared with α-tocopherol. The rationale supporting the development of γ-tocopherol as a colorectal cancer preventive agent is reviewed here.
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Therapeutic Uses of Antioxidant LiposomesStone, William L., Smith, Milton 01 December 2004 (has links)
This review will focus on the therapeutic uses of antioxidant liposomes. Antioxidant liposomes have a unique ability to deliver both lipid- and water-soluble antioxidants to tissues. This review will detail the varieties of antioxidants which have been incorporated into liposomes, their modes of administration, and the clinical conditions in which antioxidant liposomes could play an important therapeutic role. Antioxidant liposomes should be particularly useful for treating diseases or conditions in which oxidative stress plays a significant pathophysiological role because this technology has been shown to suppress oxidative stress. These diseases and conditions include cancer, trauma, irradiation, retinotherapy or prematurity, respiratory distress syndrome, chemical weapon exposure, and pulmonary infections.
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