This work deals with application of ARM based development board as platform for remote controlling of attached peripherals. It describes practical experiences with A20- OLinuXino-Micro-4GB development board and it’s available options. Used hardware and testing peripherals are described in the next part along with programming of the software for web interface and for communication in between development board and peripherals. The second half of this document describes design process of own embedded system beginning with schematic, continuing with PCB wiring. Each step of PCB wiring process is described with required details in text, including crucial connections between processor and memory chips. The last part contains details about setting a calibration of memory chips and designing optional peripheral modules.

Orientador: Licio Augusto Velloso / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-22T19:17:30Z (GMT). No. of bitstreams: 1 Bombassaro_Bruna_M.pdf: 2789722 bytes, checksum: 84c9ac89c77f8ea14c7c63e6f9bfdfc6 (MD5) Previous issue date: 2013 / Resumo: A proteína PGC1? é um co-ativador de transcrição gênica que desempenha papel importante na regulação de uma série de fenômenos metabólicos que compreendem desde o controle da termogênese e mitocondriogênese até a regulação da secreção de insulina e a produção hepática de glicose. Como vários dos fenômenos biológicos controlados direta ou indiretamente pela PGC1? tem importância vital, a regulação dos níveis de PGC1? nos tecidos deve ser finamente ajustada. Nos últimos anos, inúmeros estudos exploraram os mecanismos envolvidos com o controle da expressão gênica e tradução da PGC1?. Entretanto, apenas alguns poucos estudos avaliaram a degradação da mesma. Um dos mais importantes mecanismos envolvidos com a regulação funcional e da meia-vida de proteínas é a ubiquitinação, que pode direcionar proteínas alvo ao proteassoma para degradação ou a outras modificações pós-traducionais. O objetivo do presente estudo foi avaliar a participação de uma proteína com atividade deubiquitinase e ubiquitina ligase, a A20, na manutenção da homeostase do tecido adiposo de animais submetidos à dieta rica em gordura e voluntários humanos magros e obesos antes e após cirurgia de redução de peso. Foram utilizados o tecido adiposo branco visceral e subcutâneo e o tecido adiposo marrom de camundongos Swiss machos submetidos a 16 semanas de dieta hiperlipídica e o tecido adiposo subcutâneo de voluntários magros e obesos antes e após a cirurgia bariátrica. Esses tecidos foram avaliados quanto ao conteúdo protéico e expressão gênica da proteína A20, e sua associação com a PGC1? por imunoprecipitação e imunofluorescência, bem como a ubiquitinação desta última. Os resultados obtidos a partir do tecido adiposo de humanos mostram uma diminuição na expressão da proteína A20 nos pacientes antes e após a cirurgia bariátrica com relação aos voluntários magros. A PGC1? aparece mais ubiquitinada nos pacientes obesos em relação e a associação entre A20 e PGC1? parece aumentar com o ganho de peso na mesma proporção que o conteúdo protéico de PGC1? parece diminuir. No tecido adiposo subcutâneo de camundongos obesos, observamos uma diminuição de PGC1? bem como redução da marcação por cadeias de poliubiquitina desta proteína, associado a um aumento de A20 e aumento da associação de A20 com PGC1?. Camundongos obesos foram também tratados com um oligonucleotídeo antisense (ASO) para A20, resultando na redução de sua expressão gênica. Os animais tratados apresentaram uma piora na tolerância à glicose no teste de GTT o que ocorreu concomitantemente a redução de PGC1?. Nossos resultados indicam que, no tecido adiposo, a A20 se associa a PGC1? e a redução da sua expressão resulta em redução da expressão da PGC1? o que é acompanhando de uma piora no controle homeostático da glicose / Abstract: Peroxisome proliferator-activated receptor ? coactivator 1 alpha (PGC-1?) plays an important role in whole body metabolism and, particularly in glucose homeostasis. Its expression is tightly regulated and, small variations in tissue levels can have a major impact in a number of physiological and pathological conditions. Recent studies have shown that the ubiquitin/proteasome system plays a role in the control of PGC-1? degradation. Here we evaluated the interaction of PGC-1? with the protein A20, which plays a dual-role in the control of the ubiquitin/proteasome system acting as a deubiquitinase and as an E3 ligase. We employed immunoprecipitation, quantitative real-time PCR and immunofluorescence staining to evaluate PGC-1?, A20, PPAR? and ubiquitin in the adipose tissue of humans and mice. Our results show that, in distinct sites of the adipose tissue A20 binds to PGC-1?. At least in the subcutaneous fat of humans and mice the levels of PGC-1? decrease during obesity, while its physical association with A20 increases. The inhibition of A20 leads to a reduction of PGC-1? and PPAR? expression, suggesting that A20 acts as a protective factor against PGC-1? disposal. Thus, we provide evidence that mechanisms regulating PGC-1? ubiquitination are potentially involved in the control of the function of this transcriptional co-activator / Mestrado / Fisiopatologia Médica / Mestra em Ciências

京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第22695号 / 医博第4639号 / 新制||医||1045(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 髙橋 良輔, 教授 Shohab YOUSSEFIAN, 教授 湊谷 謙司 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

The development of microarray technology has provided an unprecedented wealth of data on gene expression in various tissue and cell types. Few studies have, however, taken full advantage of these data by selecting and then extensively characterising the functions of particular genes chosen from these microarray datasets. In this study, after analysing differentially-regulated genes revealed by microarray analysis of human mast cells activated via Fc??RI cross-linking, we chose two promising gene candidates for further research, A20 and Gem. Our group??s extensive gene expression database of major leukocytes showed that both A20 and Gem were up-regulated in other leukocyte types, and yet neither of these genes has been extensively explored in mast cells or in the immune system prior to our study. In order to investigate the first of these genes selected for further study, A20, we utilised both A20-deficient mast cells and mast cells in which A20 was over-expressed. Our findings establish for the first time that A20 is an important regulator of mast cell inflammatory responses to both LPS and Fc??RI cross-linking, and that it plays a novel role in mast cell proliferation. Our study of the second gene chosen for investigation, Gem, was conducted in a Gemdeficient mouse model developed by our group. In this study, we investigated the effect of Gem deficiency in two key immune cell types, macrophages and T-cells, complementing the work of a previous group member who investigated Gem deficiency in mast cells. Our results clearly exclude a role for Gem in macrophage and T-cell effector responses, and further establish that Gem is dispensable for in vivo inflammatory responses in models of delayed-type hypersensitivity and allergic airway inflammation. In addition to these findings, and given that the physiological role of Gem was not yet understood prior to our study, we extended our investigation to explore a potential function for Gem in the metabolic system. Using Gem-deficient mice, we found that Gem is necessary for insulin secretion from pancreatic islets. These findings confirm the potential for microarray expression data to reveal excellent gene candidates for further research and functional characterisation.

The development of microarray technology has provided an unprecedented wealth of data on gene expression in various tissue and cell types. Few studies have, however, taken full advantage of these data by selecting and then extensively characterising the functions of particular genes chosen from these microarray datasets. In this study, after analysing differentially-regulated genes revealed by microarray analysis of human mast cells activated via Fc??RI cross-linking, we chose two promising gene candidates for further research, A20 and Gem. Our group??s extensive gene expression database of major leukocytes showed that both A20 and Gem were up-regulated in other leukocyte types, and yet neither of these genes has been extensively explored in mast cells or in the immune system prior to our study. In order to investigate the first of these genes selected for further study, A20, we utilised both A20-deficient mast cells and mast cells in which A20 was over-expressed. Our findings establish for the first time that A20 is an important regulator of mast cell inflammatory responses to both LPS and Fc??RI cross-linking, and that it plays a novel role in mast cell proliferation. Our study of the second gene chosen for investigation, Gem, was conducted in a Gemdeficient mouse model developed by our group. In this study, we investigated the effect of Gem deficiency in two key immune cell types, macrophages and T-cells, complementing the work of a previous group member who investigated Gem deficiency in mast cells. Our results clearly exclude a role for Gem in macrophage and T-cell effector responses, and further establish that Gem is dispensable for in vivo inflammatory responses in models of delayed-type hypersensitivity and allergic airway inflammation. In addition to these findings, and given that the physiological role of Gem was not yet understood prior to our study, we extended our investigation to explore a potential function for Gem in the metabolic system. Using Gem-deficient mice, we found that Gem is necessary for insulin secretion from pancreatic islets. These findings confirm the potential for microarray expression data to reveal excellent gene candidates for further research and functional characterisation.

Hepatitis C virus (HCV) infection is a global health problem characterized by a high rate of chronic infection, which may in part be due to a defect in myeloid dendritic cells (mDCs). This defect appears to be remedied by treatment with interferon-α (IFN-α) -based antiviral therapies; however, the molecular mechanisms underlying mDC dysfunction in HCV infection and restoration by IFN-α treatment are unclear. The ubiquitin-editing protein A20 plays a crucial role in controlling the maturation, cytokine production and immunostimulatory function of mDCs. We propose that the expression of A20 correlates with the function of mDCs during HCV infection and IFN-α therapy. In this study, we observed that A20 expression in mDCs isolated from chronically HCV-infected subjects was significantly higher than healthy subjects or subjects achieving sustained virological responses (SVR) following antiviral treatment. Notably, A20 expression in mDCs from HCV patients during IFN-α treatment was significantly lower than for untreated patients, SVR patients, or healthy subjects. Besides, A20 expression in mDCs stimulated by polyI:C differed between HCV patients and healthy subjects, and this difference could be abrogated by the treatment with IFN-α in vitro. Additionally, A20 expression by polyI:C-activated mDCs, with or without IFN-α treatment, negatively correlated with the expression of HLA-DR, CD86 and CCR7, and the secretion of interleukin-12 (IL-12), but positively associated with the production of IL-10. Importantly, silencing A20 expression using small interfering RNAs increased the production of IL-12 in mDCs of chronically HCV-infected individuals. These findings suggest that A20 plays a crucial role in negative regulation of innate immune responses during chronic viral infection.

A Baccharis coridifolia é uma planta tóxica da família Compositae, gênero Baccharis L., conhecida popularmente como \"mio-mio\", a qual é encontrada no sul do Brasil e também em algumas regiões do Estado de São Paulo. Em bovinos, a intoxicação provoca lesões necróticas no trato gastrintestinal e nos tecidos linfóides sólidos em geral, com exceção do timo. Já em camundongos, a B. coridifolia promove, além das lesões em órgãos linfóides, lesões necróticas também no timo. Os princípios ativos da B. coridifolia foram isolados e identificados como sendo os tricotecenos macrocíclicos: roridinas A, D e E; verrucarinas A e J e miotoxina A. Em relação ao mecanismo de ação, trabalhos recentes in vitro demonstraram que as roridinas e as verrucarinas, promovem apoptose em tecido linfóide. Deste modo, considerando a toxicidade desta planta aos tecidos linfóides, o objetivo do presente estudo foi investigar se linfócitos tumorais (linfoma) seriam mais sensíveis aos efeitos tóxicos da B. coridifolia que linfócitos normais. Para tal, fez-se um experimento in vitro com culturas de linfócitos provenientes do baço e timo de camundongos hígidos, bem como células do linfoma murino A20 e carcinoma mamário de Ehrlich tratadas com o Resíduo Hexânico (RH) obtido do extrato etanólico de B. coridifolia por 24 horas. A viabilidade celular foi verificada pela técnica MTT, na qual se evidenciou diminuição da viabilidade das duas linhagens tumorais tratadas, linfoma A20 e carcinoma de Ehrlich, sem alteração na viabilidade dos linfócitos normais. Para melhor caracterizar este efeito tóxico do RH em linfócitos tumorais, camundongos nude (NMRI-nu/nu) foram inoculados com células A20, sendo posteriormente tratados por via oral, durante sete dias, com RH, associado ou não a uma aplicação intraperitoneal do quimioterápico ciclofosfamida no primeiro dia de tratamento. Resultados mostraram que os animais tratados com RH foram menos afetados com a ocorrência de metástase do linfoma primário para outros órgãos e que as involuções tumorais foram mais expressivas no grupo submetido à associação deste tratamento com uma aplicação de ciclofosfamida. Estes resultados permitem sugerir que linfócitos tumorais são mais suscetíveis aos efeitos tóxicos da B. coridifolia. / Baccharis coridifolia is a toxic plant from Compositae family, genus Baccharis L., known as \"mio-mio\". It is found in the south of Brazil, as well as in some regions of São Paulo state. In bovines, its intoxication leads to necrotic injuries to gastrointestinal tract and linfoid tissues. In mice, B. coridifolia leads, apart from linfoid solids in general to necrotic injuries in thymus. B. coridifolia\'s active principles were isolated and identified as being macrocyclic tricotecenes: roridines A, D and E; verrucarines A and J and miotoxine A. Related to action pathway, recent research (in vitro) has shown that roridines and verrucarines, B. coridifolia\'s active principles, lead to apoptosis in linfoid tissues. Therefore, considering this plant\'s toxicity to linfoid tissues, this work aimed the study of whether tumoral lymphocytes (lymphoma) would be more sensitive to B. coridifolia\'s effects than regular lymphocytes. In this sense, an experiment was carried out (in vitro) using healthy mice\'s lymphocytes from spleens and thymus, as well as cells from murine A20 lymphoma and Ehrlich breast carcinoma treated with a residue (RH, hexane obtained) from a B. coridifolia\'s ethanol effusion (24 hours). The cell viability was tested using MTT technique, where a decrease in viability was found for treated tumoral cells lineages, A 20 lymphoma and Ehrlich carcinoma, with no changes in normal lymphocytes viability. To find a better characterization to RH\'s toxic effect in tumoral lymphocytes, nude mice (NMRI-nu/nu) were inoculated with A 20 cells and then were orally treated, during 7 days, with RH, and, in some cases, with intraperitonial injection of cyclophosphamide, during the first day of treatment. Results showed that RH treated animals were less affected to primary lymphoma metastasis in other organs and tumoral involutions were more prominent in the group treated with cyclophosphamide concomitantly. These results suggest that tumoral lymphocytes are more susceptible to B. coridifolia\'s toxic effects.

This contribution examines the teaching of economics at the Hochschule für Welthandel as a case study in the evolution of Austrian academic economics in the 20th century. The period considered is divided into three periods - before, under and after the NS-regime. The main focus is on the multiparadigmatic character of the discipline before WWII, on economics under the NS rule, and on the restoration and delayed integration of economics into the international mainstream after 1945. On the personal level, the teaching of economics at the Welthandel was dominated for more than three decades by Walter Heinrich and Richard Kerschagl, whose influence is explored with regard to their academic, scientific and political activities. (author's abstract) / Series: Department of Economics Working Paper Series

A Baccharis coridifolia é uma planta tóxica da família Compositae, gênero Baccharis L., conhecida popularmente como \"mio-mio\", a qual é encontrada no sul do Brasil e também em algumas regiões do Estado de São Paulo. Em bovinos, a intoxicação provoca lesões necróticas no trato gastrintestinal e nos tecidos linfóides sólidos em geral, com exceção do timo. Já em camundongos, a B. coridifolia promove, além das lesões em órgãos linfóides, lesões necróticas também no timo. Os princípios ativos da B. coridifolia foram isolados e identificados como sendo os tricotecenos macrocíclicos: roridinas A, D e E; verrucarinas A e J e miotoxina A. Em relação ao mecanismo de ação, trabalhos recentes in vitro demonstraram que as roridinas e as verrucarinas, promovem apoptose em tecido linfóide. Deste modo, considerando a toxicidade desta planta aos tecidos linfóides, o objetivo do presente estudo foi investigar se linfócitos tumorais (linfoma) seriam mais sensíveis aos efeitos tóxicos da B. coridifolia que linfócitos normais. Para tal, fez-se um experimento in vitro com culturas de linfócitos provenientes do baço e timo de camundongos hígidos, bem como células do linfoma murino A20 e carcinoma mamário de Ehrlich tratadas com o Resíduo Hexânico (RH) obtido do extrato etanólico de B. coridifolia por 24 horas. A viabilidade celular foi verificada pela técnica MTT, na qual se evidenciou diminuição da viabilidade das duas linhagens tumorais tratadas, linfoma A20 e carcinoma de Ehrlich, sem alteração na viabilidade dos linfócitos normais. Para melhor caracterizar este efeito tóxico do RH em linfócitos tumorais, camundongos nude (NMRI-nu/nu) foram inoculados com células A20, sendo posteriormente tratados por via oral, durante sete dias, com RH, associado ou não a uma aplicação intraperitoneal do quimioterápico ciclofosfamida no primeiro dia de tratamento. Resultados mostraram que os animais tratados com RH foram menos afetados com a ocorrência de metástase do linfoma primário para outros órgãos e que as involuções tumorais foram mais expressivas no grupo submetido à associação deste tratamento com uma aplicação de ciclofosfamida. Estes resultados permitem sugerir que linfócitos tumorais são mais suscetíveis aos efeitos tóxicos da B. coridifolia. / Baccharis coridifolia is a toxic plant from Compositae family, genus Baccharis L., known as \"mio-mio\". It is found in the south of Brazil, as well as in some regions of São Paulo state. In bovines, its intoxication leads to necrotic injuries to gastrointestinal tract and linfoid tissues. In mice, B. coridifolia leads, apart from linfoid solids in general to necrotic injuries in thymus. B. coridifolia\'s active principles were isolated and identified as being macrocyclic tricotecenes: roridines A, D and E; verrucarines A and J and miotoxine A. Related to action pathway, recent research (in vitro) has shown that roridines and verrucarines, B. coridifolia\'s active principles, lead to apoptosis in linfoid tissues. Therefore, considering this plant\'s toxicity to linfoid tissues, this work aimed the study of whether tumoral lymphocytes (lymphoma) would be more sensitive to B. coridifolia\'s effects than regular lymphocytes. In this sense, an experiment was carried out (in vitro) using healthy mice\'s lymphocytes from spleens and thymus, as well as cells from murine A20 lymphoma and Ehrlich breast carcinoma treated with a residue (RH, hexane obtained) from a B. coridifolia\'s ethanol effusion (24 hours). The cell viability was tested using MTT technique, where a decrease in viability was found for treated tumoral cells lineages, A 20 lymphoma and Ehrlich carcinoma, with no changes in normal lymphocytes viability. To find a better characterization to RH\'s toxic effect in tumoral lymphocytes, nude mice (NMRI-nu/nu) were inoculated with A 20 cells and then were orally treated, during 7 days, with RH, and, in some cases, with intraperitonial injection of cyclophosphamide, during the first day of treatment. Results showed that RH treated animals were less affected to primary lymphoma metastasis in other organs and tumoral involutions were more prominent in the group treated with cyclophosphamide concomitantly. These results suggest that tumoral lymphocytes are more susceptible to B. coridifolia\'s toxic effects.

This thesis deals with electronic attendance systems which are nowadays being widely used to record employee attendance. General problem of attendance tracking is introduced in first part of the thesis along with a conceptual solution of an attendance system terminal. Author then describes a circuitry and a PCB design of a baseboard for a Cubieboard2 minicomputer, a RFID reader and a touchscreen LCD display. After successful testing of said PCB, the terminal has been completed and it was possible to focus on a software solution. Latter part of the thesis is dedicated to a specifics of the Allwinner A20 platform and its support by a GNU/Linux operating system. In following chapter author describes use of a Buildroot software for creating a Linux based binary image for an aforementioned minicomputer. Since an operating system and a peripheral support were successfully tested author could focus on a design and implementation of an attendance software that is described in the final chapter.