A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Doctor of Philosophy. August 2014. / Human immunodeficiency virus (HIV), the causative agent of the acquired immunodeficiency syndrome (AIDS), remains a topic of global concern even though great strides have been made to combat the virus. The high replicative rate of the virus and recombination of the variety of viral strains complicate the treatment of AIDS. There has been an increasing prevalence of African HIV strains in the Americas and Europe. The viral protease (PR) is vital for the propagation of the virus; and thus, is a major target in antiviral therapy. The HIV-1 PR enzyme from the subtype C strain; which predominates in sub- Saharan Africa, has been greatly under-investigated in comparison to the protease from the subtype B strain which predominates in North America and Europe. Enzyme activity data which were part of this work suggested that the South African HIV-1 subtype C protease (CSA PR) displays improved substrate turnover in comparison to the subtype B PR. Thermodynamics and inhibition kinetics of drug binding showed that the C-SA PR is less susceptible to certain clinically-used protease inhibitors when compared to the subtype B PR. A crystal structure of the C-SA PR was solved and showed no difference to the global structure of the subtype B PR. Molecular dynamics simulations showed that the C-SA PR exhibits a wider range of open conformations. Hydrogen/deuterium exchange-mass spectrometry (HDX-MS) was performed to elucidate the mechanism of reduced drug susceptibility displayed by the C-SA PR. HDX-MS data provided insights into the basis of the increased preference for open conformers displayed by the C-SA PR and the stability of the terminal dimer interface which is a target in protease inhibition.

<p>This research study attempted to highlight an in-depth exploration of my own classroom practice as a teacher at a high school in the Western Cape. A key goal of this research study was the quest for professional development and the development of an HIV/AIDS literacy programme for curriculum development.</p>

<p>This research study attempted to highlight an in-depth exploration of my own classroom practice as a teacher at a high school in the Western Cape. A key goal of this research study was the quest for professional development and the development of an HIV/AIDS literacy programme for curriculum development.</p>

Thesis (D. Phil)(Information Science)--University of Pretoria, 2004. / Includes English summary. Includes bibliographical references.

Magister Educationis - MEd / This research study attempted to highlight an in-depth exploration of my own classroom practice as a teacher at a high school in the Western Cape. A key goal of this research study was the quest for professional development and the development of an HIV/AIDS literacy programme for curriculum development. / South Africa

The aim of this study was to describe the knowledge and attitudes of college students of the Manzini Nazarene Teacher Training College with regard to HIV/AIDS. The aim was to identify risky sexual practices of the college students as well, which may require redress in order to enhance their effectiveness in combating the spread of HIV.

The aim of this study was to describe the knowledge and attitudes of college students of the Manzini Nazarene Teacher Training College with regard to HIV/AIDS. The aim was to identify risky sexual practices of the college students as well, which may require redress in order to enhance their effectiveness in combating the spread of HIV.

Magister Artium (Human Ecology) - MA(HE) / The aim of this study was to describe the knowledge and attitudes of college students of the Manzini Nazarene Teacher Training College with regard to HIV/AIDS. The aim was to identify risky sexual practices of the college students as well, which may require redress in order to enhance their effectiveness in combating the spread of HIV. / South Africa

University of Venda / MSc (Microbiology)

PhD (Health) / Department of Public Health / The Government of South Africa in response to a prevalent human immunodeficiency virus (HIV) has adopted an approach of integrating HIV/AIDS service into primary health care, as a key to achieving universal access to antiretroviral treatment (ART). Despite the government’s efforts of integrating HIV service into Primary Health Care (PHC), insufficient numbers of PHC staff and inadequate infrastructure is challenging when integrating HIV/AIDS service into PHC. This study explored the extent of HIV service integration into PHC and whether the clinic/health centre’s environment is enabling to integrate HIV service into PHC. Barriers to HIV/AIDS services integration as well as attitudes of PHC nurses were assessed. The overall purpose of this study was to develop guidelines to facilitate the integration of HIV/AIDS services into PHC in Vhembe district of Limpopo province, South Africa. An exploratory sequential mixed methods design was used. The qualitative data was collected and analysed before and results for qualitative approach used to build a subsequent quantitative phase. The current study revealed that HIV/AIDS services are integrated into every existing programme at the PHC clinic and health centres; these include: Immunisation programme, Family planning, PMTCT and ANC programmes, STIs, minor ailments and chronic illness and TB. The study further revealed that the environments at PHC clinics and health centres are not enabling the integration of HIV/AIDS services into PHC due to insufficient staff and inadequate infrastructure. Guidelines to facilitate the integration of HIV/AIDS services based on the findings was developed. The study recommendations comprise; increasing knowledge of HIV serostatus, accelerating HIV prevention, accelerating the scale-up of HIV treatment and care, creating of enabling environment for the integration of HIV/AIDS services into PHC, nursing education and training and nursing education and training.