Parrotfeather [Myriophyllum aquaticum (Vellozo) Verdecourt] is a non-native aquatic plant from South America that was introduced into the United States in the 1890's. Research was conducted to elucidate seasonal life history, starch allocation patterns, and key environmental factors that may affect plant growth. Environmental factors identified in field studies were used to develop a conceptual model to display relationships between growth and environmental factors. The conceptual model served as a broad-based hypothesis to parameterize growth limiting factors as it related to M. aquaticum growth. Mesocosm experiments were then conducted to test relationships depicted in the model and define the growth requirements of this species. Emergent shoot biomass, submersed shoot biomass, and sediment root biomass were related to light transmittance. Submersed shoot biomass was also related to water temperature. Stolons accounted for 40-95% of total biomass. Starch allocation was also greatest in stolons (78.1 g m-2); where up to 16.3% of total starch was stored. Low points in biomass and starch occurred from October to March. Biomass was greater when plants were grown in 30% shade, whereas plant length was greatest when plants were grown in 50% shade, with reductions observed in full sunlight. Biomass increased by 53% when nitrogen and phosphorus were added to the water column at 1.80 and 0.01 mg L-1, respectively. Myriophyllum aquaticum yield response was positively related (r2 = 0.82) to increasing nitrogen content and a critical concentration of 1.80% nitrogen and 0.20% phosphorus was identified for M. aquaticum growth. Plants grown at 0 cm water depth had 96% greater biomass than plants grown at water depths of 137 cm. Total length was 25% greater when plants were grown at water levels from 0-77 cm. Winter drawdowns reduced biomass by 99% at 4 weeks when compared to pre drawdown biomass. Summer drawdown efficacy was more rapid where biomass was reduced by 98% at 2 weeks when compared to pre drawdown biomass. Subsurface herbicide applications were not more efficacious than herbicides applied to the foliage. The foliar application of 2,4-D was the only herbicide and application method that resulted in ¡Ý90% biomass reduction of M. aquaticum.

Improperly designed road-stream crossings can fragment stream networks by restricting or preventing aquatic organism passage. These crossings may also be more vulnerable to high flow events, putting critical human infrastructure at risk. Climate change, which will require access to suitable habitat for species persistence, and is also predicted to increase the frequency and magnitude of extreme floods, underscores the importance of maintaining stream connectivity and resilient infrastructure. Given the large number of road-stream crossings and the expense of replacement, it is increasingly important to prioritize removals and account for the multiple benefits of these management actions. I developed an aquatic barrier prioritization scheme that combines potential habitat gain, stream thermal resilience, aquatic organism passage, and culvert risk of failure. To assess relative thermal resilience, I deployed paired air-water thermographs in several New England watersheds and analyzed relative thermal sensitivity (relationship of water to air temperature) and exposure (duration, frequency, and magnitude of warm stream temperature episodes) among streams. These were combined into a single metric of thermal resilience corresponding with the distance of that stream’s sensitivity and exposure from the watershed median. To test the relationship between risk of failure, culvert dimensions, and stream connectivity, I developed a logistic regression to predict risk of failure using data from two watersheds that experienced extreme flooding from Hurricane Irene (2011). Finally, I applied the resultant prioritization scheme to 66 road-stream crossings in the Westfield River watershed (MA). Thermal habitat quality varied considerably within and among watersheds. Stream sensitivity was generally lower than the widely accepted 0.8 ̊C increase in stream temperature for every 1 ̊C increase in air temperature (Westfield median sensitivity = 0.44), with substantial differences among streams. Exposure also varied widely among streams, indicating that some headwater streams in New England are more thermally resilient than previously thought. Risk of infrastructure failure was predicted with a logistic regression using culvert constriction ratio and predicted aquatic organism passage as predictors (Likelihood ratio test, X2=59.1, df=3, p- value=9.2e-13), indicating that underdesigned culverts were more likely to be barriers to passage and more likely to fail in extreme flow events. To prioritize culverts, this study ultimately used a piecewise approach that identified culverts opening the longest reaches of thermally resilient habitat, and then ranked those culverts by infrastructure replacement need. In the Westfield River, the prioritization clearly identified crossing replacements most likely to yield multiple benefits. The scheme I developed can accommodate changes in the relative weights of the different criteria, which will reflect differences in management and conservation concerns in the confidence of inputs. In conclusion, increasing connectivity by removing barriers may be one of the most effective ways to mitigate the effects of climate change on aquatic systems, but it is important to remove the right barriers.

Watershed systems are experiencing rapid changes to water quality and hydrologic regimes due in part to climate-induced changes in temperature and precipitation, urbanization, and increases in aquatic invasive species. Aquatic invasive species are one of the primary threats to ecosystems, contributing to loss of biodiversity, altered hydrologic regimes, and stream degradation. Urban land use and climatic factors influence the spread of invasive species, presenting greater challenges for future invasive species management. There is a need for more research that evaluates the watershed process in connection with urban land use and climate change factors in relation to invasive species spread. This study will examine factors of climate change and land use that may be influencing the spread and occurrence of aquatic invasive plants within the Connecticut River watershed. There will be four species involved in this study: Eurasian milfoil (Myriophyllum spicatum), Variable milfoil (Myriophyllum heterophyllum), Hydrilla (Hydrilla verticillate), and the European water chestnut (Trapa natans). Hydrological conditions within the watershed will be analyzed using the SWAT model through the HAWQS interface. ArcGIS Pro will be used to combine and prepare data so that it may be utilized through MaxEnt. MaxEnt will be used to create species distribution models to estimate the probability of the presence of invasive aquatic plant species in the Connecticut river watershed.

<p>Elucidating the mechanisms structuring communities has been a challenge for community ecology since its beginnings. One theory argues that assembly rules structure communities by means of deterministic mechanisms arising from biological interactions. Another view maintains that patterns seen in community composition and species abundance result from stochastic processes such as migration and extinction. The dilemma has yet not been resolved unambiguously. The main issue is that communities shaped by deterministic mechanisms can produce stochastic patterns via priority effects. The main goal of this study was to determine whether assembly rules structure communities. My strategy was to minimize priority effects by controlling timing of colonization. To do this I used a null community by combining communities of 17 rock pools. This null community was later divided among experimental communities. I conducted three experiments: (1) Experimental communities were exposed to the same external conditions. (2) Communities were exposed to different environments, disturbance, dispersal and habitat heterogeneity. (3) Replicated null communities were connected to allow inter-replicate dispersal. After 4 months, communities (experiment 1) formed alternative states, suggesting the lack of assembly rules control in community structure. The second experiment showed that adding factors results in more alternative states. The increasing number of alternative states among replicate communities indicates that diversified environment and migration are needed to reproduce qualitative patterns observed in nature. The last experiment (3) showed that patterns observed among connected replicate communities resemble patterns that emerged in the presence of biological interactions in unconnected communities. Similarity of patterns between connected and unconnected groups of communities suggest that local biological interactions can be sufficient to structure communities to a considerable degree. Nevertheless, the regional processes appear necessary in their role of supplying species for local communities.</p> / Doctor of Philosophy (PhD)

<p>Aquatic vegetation in the pristine coastal marshes of eastern Georgian Bay (GB) provides critical spawning and foraging habitat for fish species, with complex habitat supporting the greatest diversity. These wetlands are threatened by a changing water level regime and forecasted lower water levels. To monitor and conserve these wetlands, we must understand how they function and respond to this stressor. The overall goals of this thesis are to determine the impact of declining water levels on both wetland fish habitat and the fish community as well as identify the spatial scale of habitat utilization by fishes.</p> <p>We first delineate all coastal wetlands in eastern GB, identifying 3771 wetlands that provide habitat for Great Lakes fishes. Using satellite imagery, we develop an object-based classification method to classify four types of wetland vegetation. Since submerged aquatic vegetation (SAV) is not visible from satellite imagery in GB, we develop a model to predict potential area of this important habitat. The model suggests that the response of SAV to declining water levels depends on wetland geomorphology, but generally, the area of SAV decreases. To assess the response of fish habitat coverage and structure to sustained low-water levels, we classify vegetation in images collected in 2002 and 2008. The result is increasingly homogeneous habitat, a net loss of fish habitat and a decrease in fish species richness. Finally, mark-recapture and radio-tracking are used to evaluate fish movement among closely situated wetlands. Results suggest that the current distance used to group and protect small wetlands provincially (750 m), likely protects most resident fish species, but does not cover movement patterns of a top predator. This research will advance our scientific understanding of freshwater coastal ecosystems and aid in the creation of conservation strategies to mitigate future threats from declining water levels.</p> / Doctor of Philosophy (PhD)

<p>Coastal wetlands are hydrologically connected to their watershed and the lake. Water levels in Georgian Bay have been at a sustained low for thirteen years and thus connectivity of wetlands to the lake is being threatened as water levels decline. Decreased connectivity has likely caused changes in ecological and chemical characteristics. Future climate change models predict further water declines and potentially increasing the number of wetlands that will be hydrologically disconnected. The over-arching goal of this thesis is to investigate the role of connectivity between the lake and coastal marshes in eastern Georgian Bay on the amount of potential fish habitat, water chemistry and larval amphibian habitat.</p> <p>Bathymetric information is needed in order to estimate fish habitat and two approaches were utilized in order to collect these data. A site-specific method completed in 2009 used an intensive field survey in seven wetlands to create a digital elevation model and calculated the amount of fish habitat at 10 cm increments. A second, regional method, selected 103 sites by using a stratified random sample in 18 quaternary watersheds. In both methods, changes in water levels between 173 and 176 m asl resulted in the most drastic loss of habitat. Approximately 24% of the current fish habitat has already been lost due to low water levels.</p> <p>Water chemistry in coastal marshes is influenced by hydrologic connection. In the summers of 2010 and 2011, 35 coastal marshes were sampled, 17 of which had been impounded and disconnected by a beaver dam. Beaver-impounded marshes resulted in significantly lower pH, conductivity, dissolved oxygen and sulphate concentrations, but had significantly higher soluble reactive phosphors concentrations. These conditions are indicative of the lack of connection and reduced mixing with lake water. This altered habitat was shown to support breeding area for 7 species of amphibians, the most common being green frogs and the least common being American Toads and chorus frogs.</p> / Master of Science (MSc)

Made available in DSpace on 2016-12-06T17:07:10Z (GMT). No. of bitstreams: 1 DISSERTACAO_CLAUDIA(1).pdf: 520282 bytes, checksum: b361d50d0b51915379ecefc3af0487a9 (MD5) Previous issue date: 2006-08-21 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Introduction The hypertensive illness is the most dangerous factor of risk for the development of cardiovascular diseases, reaching about 16,8 million Brazilians, with equal or superior age the 40 years, being considered a problem of public health. Objective The aim of this paper was to compare the effect of a program of aquatic exercises with a similar program of exercises in the ground of twenty sessions on the variation of the arterial pressure of hypertensive individuals of the masculine sex. Materials and method 24 carrying individuals of the masculine sex of systemic arterial hypertension had participated of the study divided randomly in two groups of 12 individuals each. Group 1 (G1) was submitted to a program of aquatic exercises and group 2 (G2) was submitted to a program of exercises in land (control group). Both the groups had carried through 20 sessions of exercises. All the individuals had been submitted to an initial evaluation, the evaluations of the arterial pressure before and after each session of exercise, as well as of the arterial pressure after 10 minutes of ceased the practical one the exercises. The intensity of the exercise was monitored through the gauging of the cardiac frequency (FC), being the same one kept in 60% of the maximum FC, as well as through Escala de Borg for perceived effort. Results The average values of initial, final and 10 minutes of interruption of the exercise systolic blood pressure had after been, respectively, for G1 and G2: 166,75, 169,81, 156,04; 138,48, 132,29, 125,90. The average values of initial, final and 10 minutes of interruption of the exercise diastolic blood pressure had after been, respectively, for G1 and G2: 93,83, 94,69, 89,85; 87,71, 85,10, 81,23. Conclusion As much the aquatic exercises as the exercises in land had been efficient in the reduction of the levels of systolic and diastolic arterial pressure, being that the aquatic exercises had revealed more efficient in reduction of these (p &#8804; 0,05), although to be impossible to affirm that this therapeutical one is more efficient of what the exercises in land, a time that the G2 had levels of arterial pressure more controlled than G1. / Introdução - A doença hipertensiva é o mais perigoso fator de risco para o desenvolvimento de enfermidades cardiovasculares, acometendo cerca de 16,8 milhões de brasileiros, com idade igual ou superior a 40 anos, sendo considerada um problema de saúde pública. Objetivos - O objetivo deste estudo foi comparar os efeitos de um programa de exercícios aquáticos com um programa similar de exercícios no solo de vinte sessões sobre a variação da pressão arterial (PA) em dois grupos independentes de indivíduos hipertensos do sexo masculino. Materiais e método - Participaram do estudo 24 indivíduos do sexo masculino portadores de hipertensão arterial sistêmica (HAS), divididos randomicamente em dois grupos de 12 indivíduos cada. O grupo 1 (G1) foi submetido a um programa de exercícios aquáticos e o grupo 2 (G2) submetido a um programa de exercícios no solo (grupo controle). Ambos os grupos realizaram 20 sessões de exercícios. Todos os indivíduos foram submetidos a uma avaliação inicial, a avaliações da PA antes e após cada sessão de exercício, bem como da PA após 10 minutos de cessada a prática dos exercícios. A intensidade do exercício foi monitorada através da aferição da freqüência cardíaca (FC), sendo a mesma mantida em 60% da FC máxima, bem como através da Escala de Borg para esforço percebido. Resultados - Os valores médios de pressão arterial sistólica (PAS) iniciais, finais e após 10 minutos de interrupção do exercício foram, respectivamente, para G1 e G2: 166,75, 169,81, 156,04; 138,48, 132,29, 125,90. Os valores médios de pressão arterial diastólica (PAD) iniciais, finais e após 10 minutos de interrupção do exercício foram, respectivamente, para G1 e G2: 93,83, 94,69, 89,85; 87,71, 85,10, 81,23. Considerações finais - Tanto os exercícios aquáticos como os exercícios no solo foram eficazes na redução dos níveis de pressão arterial sistólica e diastólica, sendo que os exercícios aquáticos mostraram-se mais eficazes na redução destas (p = 0,05), apesar de ser impossível de afirmar que esta terapêutica é mais eficaz do que os exercícios no solo, uma vez que o G2 possuía níveis de pressão arterial mais controlados que G1.

As a result of research carried out within the last decade to assess the diversity of macroinvertebrates of the Salt River in the Western Cape Province, South Africa, surveys of macroinvertebrates of 20 sites on 11 selected rivers from the same mountain range source were undertaken. This was done to make a preliminary assessment of the conservation status of the rivers of this region. Aquatic insects from the orders Ephemeroptera, Odonata, Plecoptera, Megaloptera, Trichoptera and the dipteran family Simuliidae were collected using techniques to maximize the number of taxa found. The insects collected were identified to species level where possible. Water physicochemical parameters were recorded at all sites for each sampling trip to characterize these rivers and to establish a set of baseline data for future comparisons. These parameters included measurements made on site and analysis of the concentrations of all the major ions in water samples in the laboratory. Multivariate analyses including Principle Components Analysis and Canonical Correspondence Analysis were used to reveal patterns within the water physicochemistry and species distributional data. Results include the identification of 123 species from 70 genera and 30 families. A total of 31 species were found to be undescribed, of which 17 were not previously collected and are thus completely new to science. In addition, four of these species could not be placed into any known genus. The analysis of water physicochemistry showed a clear distinction between rivers of Table Mountain sandstone and Bokkeveld shale origin. Downstream effects of anthropogenic influences were discernable too. Distinct macroinvertebrate assemblages were found based upon stream order and water chemistry composition. pH proved to be the most important driver of invertebrate assemblage composition. The high levels of endemism of the macroinvertebrates found within the upper reaches of these rivers and their degree of ecological specialization make these systems a priority for the conservation of aquatic biodiversity on a national and global scale.

No description available.

Sturgeon are an ancient family of fish which have remained essentially unchanged for 200 million years, rendering them physiologically distinct from the more modern teleosts. Of the 26 known species of sturgeons all are likely endangered. North American populations have been declining steadily since the 1800s due to factors such as overharvesting, habitat alterations and increasing pollution. White sturgeon (Acipenser transmontanus), endemic to Western North America, are the largest freshwater fish on the continent. Protecting white sturgeon is of interest because nearly all Canadian populations are endangered and they are culturally and economically important. Factors such as great size, longevity, position in the food chain and benthic life style render white sturgeon particularly susceptible to bioaccumulation of toxicants. They are known to be among the most sensitive species to pollutants such as metal ions, dioxin-like compounds and endocrine disrupters. However, little is known about their susceptibility to other priority contaminants such as selenium (Se). Selenium, in its organic form selenomethionine (SeMet) has become a contaminant of particular concern as it is a known toxicant that efficiently bioaccumulates and biomagnifies in the food chain. It is also of interest as Se is an essential micronutrient that becomes toxic at only marginally greater than optimal doses. Current elevated concentrations of SeMet in white sturgeon prey, with predicted increases in anthropogenic releases, have made it a contaminant of concern for this species. It is hypothesized that increased releases of Se to aquatic environments have contributed in part to sturgeon declines; however, to date little is known about its specific effects on this species. Therefore, the purpose of the present study was to investigate the sensitivity of three year old white sturgeon to dietary SeMet and to link physiological effects to key molecular events of toxicity and to elucidate the mechanism of toxicity. Specifically, this thesis focused on oxidative stress in liver tissue as a hypothesized primary mechanism of toxicity. For 72 days sturgeon were given either a control diet of 1.4 µg Se/g feed or a diet spiked with SeMet (5.6, 22.4 or 104.4 µg Se/g feed dry mass). These doses corresponded to an uptake necessary for proper health, two environmentally relevant exposures, and a worst-case scenario for industrial Se release, respectively. A subsample of fish was taken at day 10 to investigate molecular endpoints. Within 10 days of exposure, pathological effects were observed in fish given the high dose. Occurrence of severe edema causing exophthalmos developed within 15, 23 and 52 days in high, medium and low dose group fish, respectively. There was a 54% and 22% occurrence of lethal effects in the high and medium dose groups, respectively. Se accumulated in a dose dependent manner and reached equilibrium in high dose fish after approximately 40 days. Growth, liver weight and hepatosomatic index were all significantly lower in the high dose group. Histology of 72 day liver samples showed a significant and dose dependent increase in melanomacrophage aggregates and decrease of energy stores and cell size. Food avoidance was also observed in sturgeon exposed to the high dose. To investigate oxidative stress, 10 day liver samples were tested for changes in gene expression coding for glutathione peroxidase (GPx), superoxide dismutase, catalase, glutathione S-transferase, apoptosis inducing factor and caspase 3, using real-time PCR. Only GPx was significantly induced. Day 72 liver samples were tested for the presence of lipid hydroperoxides but there were no significant differences between dose groups and controls, which shed doubt on oxidative stress being the main driver of toxicity. Taken together the data makes a strong case for the sensitivity of white sturgeon to Se accumulation and indicates a general suppression of health due to toxic levels of exposure. However, in contrast to other fish species exposed to Se, oxidative stress is not likely the main mechanism of toxicity in white sturgeon. Findings from the present study could be used for the risk assessment of sturgeon to anthropogenic Se in aquatic ecosystems.