Vga(A)LC and Msr(A) are clinically significant resistant proteins in staphylococci that confer resistance to translational inhibitors. They belong to ARE ABC-F protein subfamily, which is part of ABC transporters. Unlike typical ABC transporters, ABC-F proteins do not have transmembrane domains that are responsible for the transport of substances through the membrane. Therefore, they do not have characteristic transport function but regulatory or resistance function. Their mechanism of action on the ribosome has been described only recently, where these proteins displace the antibiotic from the ribosome. However, some aspects of their function are still unclear. For example, what is the function of the Vga(A) location on a membrane that has been detected in the membrane fraction but not in the ribosomal. In this work, using fluorescence microscopy, I observed subcellular localization of the Vga(A)LC-mEos2, Vga(A)LC-GFP and Msr(A)-eqFP650 resistant fusion proteins in live cells of S. aureus under different culture conditions . It has been shown that Vga(A)LC-GFP and Msr(A)-eqFP650 occur in a foci near the membrane. Depending on ATPase activity or the presence of an antibiotic, the localization of Msr(A)-eqFP650 in the cell changes from focal to diffuse, presumably on ribosomes, suggesting a...

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Whistle-blowers have an important role as they reveals incongruities in organizations in order to enable improvement of working conditions. However, there is a risk of reprisals against the employees when expressing their discontent, which may prevent them from blowing the whistle. Since whistle-blowers in private sectors have less protection of freedom of expression against their employer, the protections of whistleblowing also become weaker in these organizations. Nowadays it is becoming increasingly common for businesses in the welfare sector to govern under private management, but still with public funding. A weak protection of whistleblowing therefore reduces the public's opportunity to gain insight into how public funds are used. Therefore the aim of the study is to examine the protection of whistleblowing in private sectors and especially highlight the problem with a weak protection in private organizations that are publicly funded. The Swedish law has no general regulation for the protection of whistle-blowers, which leads to a complex legal situation. It will additionally be new regulations introduced in this area, which might lead to further complexity. The second purpose of the study is therefore to analyse the new regulations and identify some possible consequences of the legislation. A legal dogmatic method is used in the essay with the purpose of investigating the current legislation. The study shows that employees in private sectors have a relatively expansive right to criticize their employer and the conditions at workplaces. The judgement depends on how the employee chooses to express their criticism and therefore there are several factors that must be taking into account. The new regulations will hopefully reduce the risk of reprisals related to whistleblowing. On the other hand there are some identified weaknesses in the legislation that might imply that the intention of the law not would be fulfilled. Therefore the question still stands if the whistle-blower’s situation in the private sector will be improved by the new regulations.

I look to the common and mundane as sources for inspiration and imagination. In an attempt to evolve a veneer away from representing a coveted material, I have taken phenomenological events from my own personal experience and have searched for commonalities with them to create a new idea for veneering. My exploratory use of Google as a grammar-less machine deciphers the very nature of naming and its ambiguous attachment to an image. I am interested in the multiplicity of personal associations that become attached to images, and the subjectivity thus involved in the naming of an image.

This thesis deals with the relationship between family and school at the beginning of primary school. The theoretical part defines the matters of the cooperation between family and school, it explains terms like family, school and the relationship between them. Next it deals with historical roots of the relationship, conditions for the effective cooperation between family and school and specifications in curriculum together with some educational plans and initiatives. The practical parts presents varied research which is focused on the concrete level of the cooperation between family and school at the beginning of primary school and which is marked by the sign Parents are welcome. The evaluative instrument is Survey for parents, results of it create the base for the interview with the head teacher of the school. Complementary research finds the opinions of the teachers in the matter of the cooperation between family and school. The results of the research proved that the quality of the cooperation between the family and school at this grammar school is at high level. Parents evaluate the attitude of this school positively and they claim there is partnership between them and the school, which fulfill the standard of the sign Parents are welcome. The research also proved it is possible to get...

京都大学 / 0048 / 新制・課程博士 / 博士(薬学) / 甲第22401号 / 薬博第839号 / 新制||薬||241(附属図書館) / 京都大学大学院薬学研究科薬学専攻 / (主査)教授 金子 周司, 教授 土居 雅夫, 教授 竹島 浩 / 学位規則第4条第1項該当 / Doctor of Pharmaceutical Sciences / Kyoto University / DFAM

O aumento da produção de habitações de interesse social traz consigo a problemática da qualidade de unidades habitacionais construídas a baixo custo e em um curto espaço de tempo. Aliadas a essas preocupações de caráter qualitativo e quantitativo, surgem também as exigências de caráter ambiental para edifícios, as quais deram origem à criação de dezenas de sistemas de certificação de desempenho ambiental de edifícios, de caráter internacional, que vêm sendo usadas no cenário brasileiro, indiferentemente às suas especificidades e carências. Com isso, o objetivo deste trabalho foi promover uma análise comparativa desses sistemas de certificação, realizando uma discussão de suas inadequações ao contexto brasileiro. A partir da análise comparativa e balizamento de itens avaliativos das certificações estudadas pelos critérios preestabelecidos neste trabalho, pudemos notar que as ferramentas estudadas demonstram uma série de itens avaliativos plenamente aplicáveis a edifícios residenciais situados no cenário brasileiro e outros que ainda necessitam de adaptações. Portanto, esta análise deverá servir como embasamento para um trabalho futuro de criação de diretrizes para o desenvolvimento de um sistema de certificação ambiental de edificações habitacionais brasileiras. / The increased production of social housing brings the issue of the quality of housing units built at low cost. Combined to these concerns, those are qualitative and quantitative, are also the character of environmental requirements for buildings which led to the creation of dozens of buildings environmental performance certification systems, with international character, which have been used in the Brazilian scene, regardless of their specificities and needs. Therefore, the objective of this work is to promote an analysis of these certification systems and conducting a discussion of their inadequacies to the Brazilian context. From the comparative analysis and marking of the certification evaluation items by the established criteria studied in this work, we noted that the studied tools show several evaluative items fully applicable to residential buildings located in Brazil and others ones that still need to be adapted. Therefore, this analysis should serve as a basis for a future work to establish guidelines for the development of an environmental certification system for residential buildings in Brazil.

This thesis aims to investigate the performance of different types of franchisees, in terms of perceived market share and profitability, are affected by constrains defined by the theories of franchise selection and contractual terms. The two different types of franchisees researched include novice and parallel. This begins by examining how the constraints defined by the Resource Base, Social Exchange and Equity theories of franchise selection are applicable to entrepreneurs who want to become franchisees, and by examining the differences in the attitude of franchisees toward contractual terms. The main contribution is that the research broadens and extends other franchising and entrepreneurship studies by exploring external and internal factors to examine perceived franchisee performance. Therefore, this study relates the criteria used by franchisees in the selection of potential franchisors and contract clauses, to their performance in the Kingdom of Saudi Arabia, and the Sultanate of Oman in the food and beverage sector. Moreover, existing research has primarily focused on the perspective of franchisors and has not adequately explored the franchisee's perspective on determining success. Therefore, this study contributes to franchising literature by broadening the scope of existing theories. The study has adopted a multi-methodology strategy, employing a combination of quantitative and qualitative approaches. Qualitative data was collected through 11 face-to-face interviews, 5 franchisees in Oman, and 6 in Saudi Arabia; using semi-structured questionnaires. Quantitative data was collected via a survey. A self-administrated questionnaire was designed, translated, piloted and distributed in Riyadh, Saudi Arabia. Data analysis was conducted on a sample of 123 responses. Factor analysis was implemented to test the scale, followed by the Mann-Whitney U test, regression, and moderation analysis. The qualitative findings show that constraints defined by the resource-based theory are applicable to novice franchisees. However, franchisor training and support were found to be exceptional resources, applicable to both novice and parallel franchisees. Constraints defined by the social exchange theory are applicable to both novice and parallel franchisees; whereas constraints defined by the equity theory are applicable only to parallel franchisees. The quantitative findings indicate that both types of franchisee have different attitudes toward training clauses; support clauses; contract duration clauses; territorial exclusivity clauses; tying arrangement clauses and franchise fees clauses. However, both types of franchisees have similar attitudes toward termination clauses. Moreover, the results show that franchisor brand reputation, technical know-how and high franchise fee factors will increase the franchisee perceived market share. Additionally, franchisor brand reputation and higher franchise fees, will increase franchisee perceived profitability. Furthermore, parallel franchisees believe that a franchisor's local market knowledge will decrease their performance in terms of perceived profitability. Alternatively, novice franchisees believe long contract duration will help them increase their perceived profitability. In addition, imposing higher franchise fees on novice franchisees will increase their perceived profitability more than that of parallel franchisees. Finally, the results show that contract clauses such as: extensiveness of support; long contract duration, and stricter termination clauses, will affect franchisee perceived market share positively. Finally, contract clauses such as extensiveness of support and short contract duration clauses, will affect franchisee perceived profitability positively; whereas extensiveness of training clauses will affect franchisee perceived profitability negatively.

As Proteínas Inativadoras de Ribossomos (RIPs) compreendem uma família de enzimas que inibem a síntese protéica através da depurinação de uma adenina específica do RNA ribossomal. Os membros desta família são classificados como RIPs do tipo I, quando possuem somente o RNA-N-Glicosidase e RIPs do tipo II quando além do domínio glicosidase, também apresentam um domínio de lectina. As RIPs foram mais estudadas em plantas, onde a ricina e a aglutinina, ambas RIP do tipo II de mamona (Ricinus communis), estão entre as primeiras descritas. O presente trabalho teve o objetivo de identificar parálogos da ricina e aglutinina, bem como RIPs do tipo I de mamona e analisar as suas relações filogenéticas. Além disso, validar o uso de 14 potenciais genes de referência para qRT-PCR em cinco estádios do desenvolvimento da semente de mamona. O padrão de expressão gênica por RT-qPCR de todas RIPs de mamona identificadas, também foram analisados nestes mesmos estádios. Um total de 18 genes de RIPs foi identificado em mamona (Rcom RIPs), dos quais 10 foram classificados como do tipo II e 8 do tipo I. As topologias das árvores filogenéticas sugerem que as Rcom RIPs foram originadas a partir de múltiplos eventos de duplicação gênica. Dois modelos evolutivos foram propostos para a radiação das Rcom RIPs, baseados em processos de fusão gênica associado ou não a eventos de duplicação parcial. Os genes Act 2/7, EF β, Ubi, TIP e UBC foram os que apresentaram perfil de expressão mais estável e foram selecionados para subsequente normalização dos dados de expressão das Rcom RIPs. Os genes que codificam as Rcom RIPI 3, 4, 5, 7 e 8 e as Rcom RIPII 1, 2, 4, 5, 6 e 8 são transcritos em sementes, sendo que a Rcom RIPII 1 (ricina) e a Rcom RIPII 2 (aglutinina) foram as mais expressas. O presente trabalho apresenta um modelo evolutivo das Rcom RIPs, o qual pode ser extrapolado para outras espécies de plantas. Este trabalho também demonstra o primeiro esforço para a padronização de genes de referência para RT-qPCR em mamona e o primeiro que apresenta a expressão outras Rcom RIPs, além da ricina e aglutinina. / Ribosome inactivating proteins (RIPs) comprise a family of enzymes that inhibit protein synthesis, after depurination of an adenine-specific ribosomal RNA. The members of this family are classified as type I RIPs, which have a RNA-Nglycosidase domain and type II RIPs encompassing a RNA-N-glycosidase and a lectin domain.The RIPs were more studied in plants, where ricin and agglutinin, both type II RIP of castor bean (Ricinus communis), were the first to be described. This work aimed to: 1) identifine paralogous of ricin and agglutinin, as well as the type I RIPs of castor bean; 2) analyze their phylogenetic relationships; 3) validate the use of 14 potential housekeeping genes for qRT-PCR for five developmental stages of R. communis seeds; 4) analyze the pattern of gene expression by RTqPCR of all RIPs castor identified in these same stages. A total of 18 genes that encode RIPs were identified in castor bean (Rcom RIPs), 10 of which were classified as type II and 8 as type I. The phylogenetic trees topologies suggest that Rcom RIPs were originated from multiple events of gene duplications. Two evolutionary models have been proposed for the radiation of Rcom RIPs based on gene fusion processes associated or not to events of partial duplication. The genes Act 2/7, EF β, Ubi, TIP and UBC presented the more stable expression profile and were selected for further RT- qPCR normalization experiments. The Rcom RIPI 3, 4, 5, 7 and 8 and Rcom RIPI 1, 2, 4, 5, 6 and 8 genes are actively transcribed in seeds, whereas the Rcom RIPI 1 (ricin) and Rcom RIPI 2 (agglutinin) were the most expressed. This paper presents an evolutionary model of Rcom RIPs, which can be extrapolated to other plant species. Also, corresponds to the first effort to standardize housekeeping genes for RT-qPCR in castor bean and the first that shows the expression Rcom RIPs, other than ricin and agglutinin.

Mes travaux ont porté sur l'étude de deux familles de protéines de liaison à l'ARN, la famille des ARE-BP (AU-rich elements binding protein) et la famille des PTB (Polypyrimidine tract binding protein) au cours du développement chez le xénope. L'étude de l'expression de cinq membres de la famille ARE-BP a mis en évidence une redondance d'expression tissulaire et temporelle entre quatre de ces ARE-BP (AUF1, KSRP, HuR et TIA1). A l'inverse, l'expression atypique de TTP a permis de suggérer son implication dans l'hématopoïèse. Mes travaux sur la famille PTB (PTBP1, PTBP2, PTBP3) ont montré que chacun des paralogues présente une expression spécifique ce qui suggère qu'elles aient des fonctions différentes lors du développement. Des résultats du laboratoire montraient que l'inactivation de PTBP1 ou de EXOSC9, un composant de l'exosome ARN, entraînait des défauts de morphogenèse de l'épiderme dorsal. Afin d'identifier l'origine moléculaire de ces défauts, j'ai réalisé l'analyse transcriptomique par séquençage à haut débit (RNA-Seq) des morphants PTBP1 et EXOSC9. J'ai produit des banques d'ADNc à partir des morphants ou d'embryons témoins et celles-ci ont été séquencées au Génoscope. L'analyse d'une cible connue de PTBP1 a montré que des modifications minoritaires de l'épissage étaient détectées à partir de ces données. De plus ces défauts d'épissage ne sont pas retrouvés dans les morphants EXOSC9, validant son utilisation comme crible additionnel permettant d'exclure les évènements d'épissage qui ne sont pas impliqués dans le défaut d'épiderme. Une approche gène candidat a été initiée afin de cibler l'analyse de transcrits impliqués dans la morphogenèse de l'épiderme dorsale.