Made available in DSpace on 2017-07-10T14:38:19Z (GMT). No. of bitstreams: 1 Cibelly Benke.pdf: 983838 bytes, checksum: b20762b1e8b6fbc90e8e2baa97175df8 (MD5) Previous issue date: 2013-03-25 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The aim was not to create a standardized regional sampling of aquatic macroinvertebrates, by drawing curves sampling sufficiency in streams with different types of diversity of these organisms. Were collected in nine streams of small order, in which 20 samples were collected, pooled and homogenized in a separate tray in 24 squares for analysis of sample sufficiency. 999 subsamples were taken randomly selected at random for each of the 23 squares on each stream analyzed. For each subsample data were taken from the total wealth of households (Ssub) and coefficient of correlation (r) between the abundances of total subsample (Asub) and sample (Aamo). Correlation analysis was used to verify that the quantities of squares determined for each stream were correlated with the Shannon diversity index. Qualitatively, it took an average of 16 squares to reach the criterion of sufficiency under sampled, while in quantitative terms, the sufficiency under sampled occurred on the seventh square. The positive relationship between diversity and subsampling of these organisms allows us to conclude that in environments where diversity is unknown, it will be necessary to draw up a "pilot study" for the use of this methodology. Thus, future work will find the number of subsamples required to be screened, as we prepared a table with the values of diversity and their respective values of sampling sufficiency (subsamples) / O objetivo deste trabalho foi criar uma padronização não regional de amostragem de macroinvertebrados aquáticos, através da elaboração de curvas de suficiência amostral em riachos com diferentes tipos de diversidade destes organismos. Foram realizadas coletas em nove riachos de pequena ordem, nos quais 20 amostras foram coletadas, agrupadas e homogeneizadas em uma bandeja separada em 24 quadrados para posterior análise de suficiência amostral. Foram obtidas 999 subamostras sorteadas ao acaso para cada um dos 23 quadrados analisados em cada riacho. Para cada subamostra foram tomados dados de riqueza total de famílias (Ssub) e o coeficiente de correlação de Pearson (r) entre as abundâncias totais da subamostra (Asub) e da amostra (Aamo). A análise de correlação foi utilizada para verificar se as quantidades mínimas de quadrados determinadas para cada riacho foram correlacionados com o índice de diversidade de Shannon. Em termos qualitativos, foram necessários, em média, 16 quadrados para atingir o critério de suficiência subamostral, enquanto que em termos quantitativos, a suficiência subamostral ocorreu já no sétimo quadrado. A relação positiva entre a diversidade e a subamostragem destes organismos nos permite concluir que em ambientes cuja diversidade seja desconhecida, será necessária a elaboração de um estudo-piloto para o uso desta metodologia. Dessa forma, trabalhos futuros poderão encontrar a quantidade de subamostras necessárias a serem triadas, pois elaboramos uma tabela com os valores da diversidade e seus respectivos valores de suficiência amostral (subamostras)

O presente trabalho testou o efeito da frequência, intensidade e tamanho da área de perturbações físicas sobre a comunidade de macroinvertebrados aquáticos, visando responder as seguintes questões: 1) a abundância, riqueza e composição dos macroinvertebrados são afetadas pelas perturbações físicas? 2) os resultados encontrados se enquadram nas predições da hipótese da perturbação intermediária (HPI) ou do modelo de equilíbrio dinâmico (MED)? Para responder estas questões manipulações experimentais do substrato (frequência, intensidade e tamanho da área de perturbação) foram realizadas e foram avaliados os seus efeitos sobre a fauna de macroinvertebrados aquáticos de um riacho de Mata Atlântica do Sudeste do Brasil. Os nossos resultados mostraram que as perturbações físicas não influenciaram a abundância e a riqueza da fauna, não se encaixando nas predições da HPI e do modelo de equilíbrio dinâmico MED. Por outro lado, a frequência afetou a composição faunística sugerindo que alguns grupos demandam um maior tempo para colonizar o substrato. Apesar de reconhecermos que alguns poucos grupos são afetados pela frequência de perturbação, a maior parte da fauna do riacho estudado deve ter uma alta mobilidade e resiliência talvez como um reflexo do alto dinamismo deste riacho, no qual é possível observar que o substrato se move em maior ou menor grau dependendo das intensidades das chuvas. Os nossos resultados sugerem que as perturbações experimentais criaram manchas desocupadas, as quais foram rapidamente ocupadas pela maior parte da fauna de macroinvertebrados aquáticos. Portanto, a capacidade de colonização dos diferentes táxons e a redistribuição entre manchas ocupadas e desocupadas devem ter um papel fundamental na dinâmica da fauna do riacho estudado. / The present work tested the effects of frequency, intensity and disturbance area on the aquatic macroinvertebrate assemblages, in order to answer the following questions: 1) do the physical disturbances affect the abundance, richness and composition of macroinvertebrate assemblages? 2) Do the finding results confirm the intermediate-disturbance hypothesis (HPI) or dynamic-equilibrium model (MED) predictions? To answer these questions, experimental manipulation of substrate (frequency, intensity and size of disturbed area) were made and their effects on aquatic macroinvertebrate fauna in an Atlantic Forest stream (Southeast of Brazil) were evaluated. The abundance and richness were not influenced by the physical disturbance and this result was not in accordance with predictions of the HPI and MED. On the other hand, the frequency affected the faunal composition suggesting that some groups demand a longer period to colonize the substrate. Although, we recognize that some groups are affected by the disturbance frequency, the most part of the stream fauna should have a higher mobility and resilience, maybe as a reflect of the higher dynamism of this stream, in which is possible to observe that the substrate moves in a higher or lower rate depending on the rain intensity. Our results suggest that the experimental disturbance generate unoccupied patches which were promptly occupied by the major part of the taxa of aquatic macroinvertebrate fauna. Therefore, the colonization capacity of the different taxa and the redistribution between occupied and unoccupied patches should have a fundamental role on the fauna dynamics in the studied stream.

Many insects form close relationships with microbial symbionts. Insect symbionts can provide novel phenotypes to their hosts, including influencing dietary breadth. In the polyphagous cowpea aphid, Aphis craccivora, the facultative symbiont Arsenophonus improves aphid performance on one host plant (locust), but decreases performance on other plants. The goal of my thesis was to investigate the mechanism by which Arsenophonus facilitates use of locust. First, I assembled an Aphis craccivora-Arsenophonus-Buchnera reference transcriptome to conduct RNAseq analysis, comparing gene expression in aphids feeding on locust and fava, with and without Arsenophonus infection. Overall, few transcripts were differentially expressed. However, genes that were differentially expressed mapped to a variety of processes, including metabolism of glucose, cytoskeleton regulation, cold and drought regulation, and B-vitamin synthesis. These results imply that Arsenophonus is producing B-vitamins, which might be deficient in locust. In a second set of experiments, I used qPCR to test whether symbiont function across host plants might be mediated by bacterial titer. I measured relative Arsenophonus abundance across plants, and found Arsenophonus titer was variable, but generally greater on locust than fava. In summary, my results suggest that Arsenophonus synthesis of B-vitamins should be further investigated and may be mediated by bacterial titer.

Natural abundance of 15N varies greatly and unpredictably within and between environments. The unpredictable nature of 15N limits the use of N isotope natural abundance (d15N) in tracing the flow and fate of N in environments. Recent investigations have, however, revealed consistent and repeatable patterns of 15N in some ecosystem components. These patterns suggest that d15N may yet provide a tool to investigate and illuminate ecosystem N cycling processes. Identifying and quantifying the sources of isotopic variation must precede any significant advance in the application of this technique, and to this end an assessment of isotopic variation associated with major ecosystem components has been carried out in this thesis. d15N patterns have been established, hypotheses proposed and tested, and conclusions about the application of the technique are presented. 15N patterns in surface and groundwater were measured in a variety of different land-use catchments in an attempt to identify distinct isotopic 'fingerprints'. High levels of 15N variation were measured in both stream and groundwaters, resulting in strongly overlapping land-use 'fingerprints'. Environmental 15N variation in streams and groundwaters was found to be too great to differentiate between land-uses based on d15N alone. In contrast, the artificially 15N enriched signature of effluent N was used to trace its flow and fate, following irrigation, in a forested catchment. The effluent d15N signature allowed it to be traced into the major ecosystem components, permitting a first order N budget to be determined for effluent N storage and loss. N sources with significantly different 15N signatures to that of 'background ecosystem N' can therefore be used to trace the flow and fate of N in ecosystems. During the course of this work a number of higher and lower order plants were observed to have highly depleted (lt; -8 ) d15N signatures. Epiphytes and lithophytes, strongly reliant on atmospheric N sources, were consistently depleted in 15N, with signatures as low as -24 , measured in a range of environments. A similar level of depletion was measured in a wide range of plants growing in early primary succession sites (as low as -22.3 ), which could not be accounted for by any abiotic or biotic factor or significantly depleted N source. The absence of any measurable driver of depletion suggested a universal fractionating mechanism which acts in a wide range of environments and vegetation types. Diffusive uptake of atmospheric NH3(g) and the proportional uptake of a supplied N source were two proposed mechanisms that could theoretically account for the level and universal nature of depletion. Diffusive uptake of atmospheric NH3(g) was tested as a primary fractionating mechanism in plants. Strongly N deficient plants were capable of utilising NH3(g) as a nutritional source, but the level of 15N depletion measured in these plants closely approximated that of the inherent NH3(g) d15N signature. No significant additional fractionation is associated with NH3(g) diffusive uptake. Diffusive uptake of atmospheric NH3(g) by plants cannot alone account for the level of depletion measured in early primary succession plant communities. Proportional uptake of a N source as a primary fractionating mechanism was tested by growing plants in various concentrations and rates of applied N. Fractionation attributed to the proportional uptake of a supplied N source, as a consequence of P limitation or rapid flow over roots, resulted in a significant level of 15N depletion in plants. The level of depletion attributed to this mechanism was, however, not sufficient to account for the level measured in early primary succession plant communities. Individual 15N fractionating mechanisms cannot alone explain the level of depletion observed in early primary succession plants, however a combination of fractionating mechanisms can. Fractionation attributed to the proportional uptake of an already depleted N source, i.e., wet deposited N, largely accounts for the level of depletion measured in early succession plant communities. This two-step fractionation model can act on both higher and lower plants, independent of ecosystem biotic and abiotic factors. Additional, and less dramatic fractionations attributed to atmospheric NH3(g) uptake, mycorrhizal associations, internal remobilisation, and taxon-specific N acquisition strategies, will contribute to the level of d15N depletion. This thesis presents the first extensive survey of highly depleted d15N signatures in terrestrial vegetation. Furthermore, thorough testing of theoretically plausible mechanisms has resulted in a full account of the highly depleted d15N signatures measured in a wide range of vegetation types and environments.

<p>Soils of Northern Hemisphere forests contain a large part of the global terrestrial carbon (C) pool. Even small changes in this pool can have large impact on atmospheric [CO2] and the global climate. Soil respiration is the largest terrestrial C flux to the atmosphere and can be divided into autotrophic (from roots, mycorrhizal hyphae and associated microbes) and heterotrophic (from decomposers of organic material) respiration. It is therefore crucial to establish how the two components will respond to changing environmental factors. In this thesis I studied the effect of elevated atmospheric [CO2] (+340 ppm, ¹³C-depleted) and elevated air temperature (2.8-3.5 oC) on soil respiration in a whole-tree chamber (WTC) experiment conducted in a boreal Norway spruce forest. In another spruce forest I used multivariate modelling to establish the link between day-to-day variations in soil respiration rates and its δ¹³C, and above and below ground abiotic conditions. In both forests, variation in δ¹³C was used as a marker for autotrophic respiration. A trenching experiment was conducted in the latter forest in order to separate the two components of soil respiration. The potential problems associated with the trenching, increased root decomposition and changed soil moisture conditions were handled by empirical modelling. The WTC experiment showed that elevated [CO2] but not temperature resulted in 48 to 62% increased soil respiration rates. The CO2-induced increase was in absolute numbers relatively insensitive to seasonal changes in soil temperature and data on δ¹³C suggest it mostly resulted from increased autotrophic respiration. From the multivariate modelling we observed a strong link between weather (air temperature and vapour pressure deficit) and the day-to-day variation of soil respiration rate and its δ¹³C. However, the tightness of the link was dependent on good weather for up to a week before the respiration sampling. Changes in soil respiration rates showed a lag to weather conditions of 2-4 days, which was 1-3 days shorter than for the δ¹³C signal. We hypothesised to be due to pressure concentration waves moving in the phloem at higher rates than the solute itself (i.e., the δ¹³C–label). Results from the empirical modelling in the trenching experiment show that autotrophic respiration contributed to about 50% of total soil respiration, had a great day-to-day variation and was correlated to total soil respiration while not to soil temperature or soil moisture. Over the first five months after the trenching, an estimated 45% of respiration from the trenched plots was an artefact of the treatment. Of this, 29% was a water difference effect and 16% resulted from root decomposition. In conclusion, elevated [CO2] caused an increased C flux to the roots but this C was rapidly respired and has probably not caused changes in the C stored in root biomass or in soil organic matter in this N-limited forest. Autotrophic respiration seems to be strongly influenced by the availability of newly produced substrates and rather insensitive to changes in soil temperature. Root trenching artefacts can be compensated for by empirical modelling, an alternative to the sequential root harvesting technique.</p>

The deep ocean (> 200-m depth) covers more than 65 percent of earth's surface and is known as the largest active carbon sink of the planet. Photosynthesis fixes inorganic carbon into organic rich-compounds to fuel the biological production in the upper ocean. A small portion of the photosynthetic carbon eventually sinks to the seafloor to support diverse deep-sea life. In this dissertation, the phytoplankton production and export flux of particulate organic carbon (POC) to the seafloor were linked to standing stocks and compositional changes of the deep-sea soft bottom assemblages. The pattern and processes of energy transfer from the surface ocean to the deep sea was examined by modeling the global benthic bacteria, meiofauna, macrofauna, and megafauna biomass from remotely sensed ocean color images and the seafloor relief. The analysis was then scaled down to the macrofauna of the Gulf of Mexico (GoM) to examine the global pattern on regional oceanic features with contrasting productivity regimes. These results suggested a universal decline of benthic standing stocks down the continental margins that is caused by an exponential decrease of export POC flux with depth. A revisit of historical epibenthic invertebrate sampling in the North Atlantic showed that both individual species and multi-species assemblages occurred in narrow depth bands that hugged the topography from the upper continental slope out to the Hatteras Abyssal Plain. The continuum compositional change suggested that the continuous decline of benthic food supply with depth was the potential driving force for the pattern of bathymetric faunal zonation. A broad, systematic survey across multiple depth transects in the northern GoM suggested that macrofauna zonation is not only taking place across isobaths, but also form the northeast to the northwest GoM due to a horizontal productivity gradient created by the nutrient-laden Mississippi River. Analyses of long-term demersal fish data from 1964 to 2002 in the northern GoM showed no evidence of large-scale faunal change across different sampling times. Base on the pooled data, a shift in rate of fish species replacement may be caused by complex biological interactions or changes in environmental heterogeneity along depth or productivity gradients.

The arctic is currently experiencing some of the greatest rates of warming. Newly emerging diseases in the arctic are of particular interest due to the implications these may have at southern latitudes if temperatures continue to rise around the globe. It is important to document changes in pathogen populations, such as alterations in range, virulence, prevalence, and abundance, and the effect these may have on their host populations. Parasites influence the reproductive success of their hosts in some cases. Studies on impacts of ectoparasites on avian reproductive success have generally been focused on species with altricial young. I studied the abundance of an apparently newly emerging nest-parasite and the effects of this parasite on Rosss (<i>Chen rossii</i>) and lesser snow goose (<i>Chen caerulescens caerulescens</i>) reproductive success in the Karrak Lake goose colony, Nunavut, Canada from 2001 to 2004. <p>The nest parasite, identified as the flea <i>Ceratophyllus vagabundus vagabundus</i>, was associated with goose eggs covered with spots of blood. The proportion of goose egg-shells covered by blood was positively correlated with flea abundance in the nest. This relationship allowed the use egg blood-coverage as an index of flea abundance for remaining analyses. Flea abundance in goose nests was associated with variables associated with the host and the hosts habitat. I used general linear models in conjunction with Akaikes information criterion (AIC) to determine which factors were most important in influencing flea abundance in goose nests. The most parsimonious model to explain the relationship between egg blood coverage and flea abundance in goose nests included goose clutch size, age of nest bowl (new vs. old), history of nesting by geese on a specific plot within the colony, habitat within 0.5m of nest, and year. The best predictor of flea abundance was the age of the nest bowl, with nest bowls re-used by geese containing more fleas than new bowls. This relationship was expected as fleas over-wintered in goose nests at the Karrak Lake colony.<p> Logistic regression and AIC were used to determine whether egg blood-coverage was an important variable influencing nest success. All top five models included blood-coverage. Goose nest success was negatively influenced by fleas in most years. There was a threshold of egg blood-coverage at which nest success was affected, and this threshold varied, with >20% blood indicating a significant decline in nest success in two years, and >5% blood-coverage indicating a decrease in nest success in one year. To my knowledge, this is the first study that has examined the parasites of avian nests in an arctic ecosystem and was also the first to investigate the effect of nest parasites on birds with precocial young. More research is needed to determine what factors limit this flea population and whether fleas may become a regulating factor for geese in the Karrak Lake colony.

Intercropping, defined as two or more crops grown on the same land area at the same time, is a sustainable alternative to sole crops. Intercropping has been associated with multiple benefits, such as increased nutrient and soil organic carbon (SOC) cycling, decreased soil erosion and increased carbon (C) sequestration. A common intercropping practice is to integrate cereal and legume crops such as maize (Zea mays L.), and soybean (Glycine max (L.) Merr.). Most studies on intercropping have focused on yield, weed control, and land use efficiency in the tropics. Few studies have researched C and nitrogen (N) dynamics in temperate intercrops, with respect to soybean and maize residue stabilization. Soil from Balcarce, Argentina, was incubated for 140 days with soybean, maize, or no residue. Throughout the incubation, results illustrated the effect of residue application upon the soil, specifically through significantly higher amounts of light fraction (LF) C and LFN concentrations, soil microbial biomass (SMB) C and SMBN concentrations, higher microbial diversity, lower N2O production rates, in addition to distinct isotopic values in soil fractions and CO2 (p<0.05). Furthermore, it was observed from δ15N-TN and δ15N-LF that treatments with soybean residues included had higher N cycling (p<0.05), emphasizing the importance of including N-fixing legumes in complex agroecosystems. Significant changes over time in SMB and SMCS characteristics, and isotope values (p<0.05) indicated the preferential utilization of relatively young and easily accessible litter. Furthermore, the loss of labile material over the incubation resulted in more recalcitrant forms (such as older C and lignin) to be utilized. Slightly higher SOC, TN, LFC and LFN concentrations, as well as lower CO2 production rates suggested 2:3 (rows of maize:rows of soybean) as a more desirable intercrop design for C sequestration. The 1:2 intercrop design was observed to be more beneficial for microbial community structure, furthering the idea that intercropping is a beneficial alternative to sole cropping. This study improves knowledge in residue stabilization and C sequestration in complex agroecosystems, providing encouragement for the implementation of more sustainable management practices.

The thesis parts from the view that statistics starts with data, and starts by introducing the data sets studied: marine benthic species counts and chemical measurements made at a set of sites in the Norwegian Ekofisk oil field, with replicates and annually repeated. An introductory chapter details the sampling procedure and shows with reliability calculations that the (transformed) chemical variables have excellent reliability, whereas the biological variables have poor reliability, except for a small subset of abundant species. Transformed chemical variables are shown to be approximately normal. Bootstrap methods are used to assess whether the biological variables follow a Poisson distribution, and lead to the conclusion that the Poisson distribution must be rejected, except for rare species. A separate chapter details more work on the distribution of the species variables: truncated and zero-inflated Poisson distributions as well as Poisson mixtures are used in order to account for sparseness and overdispersion. Species are thought to respond to environmental variables, and regressions of the abundance of a few selected species onto chemical variables are reported. For rare species, logistic regression and Poisson regression are the tools considered, though there are problems of overdispersion. For abundant species, random coefficient models are needed in order to cope with intraclass correlation. The environmental variables, mainly heavy metals, are highly correlated, leading to multicollinearity problems. The next chapters use a multivariate approach, where all species data is now treated simultaneously. The theory of correspondence analysis is reviewed, and some theoretical results on this method are reported (bounds for singular values, centring matrices). An applied chapter discusses the correspondence analysis of the species data in detail, detects outliers, addresses stability issues, and considers different ways of stacking data matrices to obtain an integrated analysis of several years of data, and to decompose variation into a within-sites and between-sites component. More than 40 % of the total inertia is due to variation within stations. Principal components analysis is used to analyse the set of chemical variables. Attempts are made to integrate the analysis of the biological and chemical variables. A detailed theoretical development shows how continuous variables can be mapped in an optimal manner as supplementary vectors into a correspondence analysis biplot. Geometrical properties are worked out in detail, and measures for the quality of the display are given, whereas artificial data and data from the monitoring survey are used to illustrate the theory developed. The theory of display of supplementary variables in biplots is also worked out in detail for principal component analysis, with attention for the different types of scaling, and optimality of displayed correlations. A theoretical chapter follows that gives an in depth theoretical treatment of canonical correspondence analysis, (linearly constrained correspondence analysis, CCA for short) detailing many mathematical properties and aspects of this multivariate method, such as geometrical properties, biplots, use of generalized inverses, relationships with other methods, etc. Some applications of CCA to the survey data are dealt with in a separate chapter, with their interpretation and indication of the quality of the display of the different matrices involved in the analysis. Weighted principal component analysis of weighted averages is proposed as an alternative for CCA. This leads to a better display of the weighted averages of the species, and in the cases so far studied, also leads to biplots with a higher amount of explained variance for the environmental data. The thesis closes with a bibliography and outlines some suggestions for further research, such as a the generalization of canonical correlation analysis for working with singular covariance matrices, the use partial least squares methods to account for the excess of predictors, and data fusion problems to estimate missing biological data.

The arctic is currently experiencing some of the greatest rates of warming. Newly emerging diseases in the arctic are of particular interest due to the implications these may have at southern latitudes if temperatures continue to rise around the globe. It is important to document changes in pathogen populations, such as alterations in range, virulence, prevalence, and abundance, and the effect these may have on their host populations. Parasites influence the reproductive success of their hosts in some cases. Studies on impacts of ectoparasites on avian reproductive success have generally been focused on species with altricial young. I studied the abundance of an apparently newly emerging nest-parasite and the effects of this parasite on Rosss (<i>Chen rossii</i>) and lesser snow goose (<i>Chen caerulescens caerulescens</i>) reproductive success in the Karrak Lake goose colony, Nunavut, Canada from 2001 to 2004. <p>The nest parasite, identified as the flea <i>Ceratophyllus vagabundus vagabundus</i>, was associated with goose eggs covered with spots of blood. The proportion of goose egg-shells covered by blood was positively correlated with flea abundance in the nest. This relationship allowed the use egg blood-coverage as an index of flea abundance for remaining analyses. Flea abundance in goose nests was associated with variables associated with the host and the hosts habitat. I used general linear models in conjunction with Akaikes information criterion (AIC) to determine which factors were most important in influencing flea abundance in goose nests. The most parsimonious model to explain the relationship between egg blood coverage and flea abundance in goose nests included goose clutch size, age of nest bowl (new vs. old), history of nesting by geese on a specific plot within the colony, habitat within 0.5m of nest, and year. The best predictor of flea abundance was the age of the nest bowl, with nest bowls re-used by geese containing more fleas than new bowls. This relationship was expected as fleas over-wintered in goose nests at the Karrak Lake colony.<p> Logistic regression and AIC were used to determine whether egg blood-coverage was an important variable influencing nest success. All top five models included blood-coverage. Goose nest success was negatively influenced by fleas in most years. There was a threshold of egg blood-coverage at which nest success was affected, and this threshold varied, with >20% blood indicating a significant decline in nest success in two years, and >5% blood-coverage indicating a decrease in nest success in one year. To my knowledge, this is the first study that has examined the parasites of avian nests in an arctic ecosystem and was also the first to investigate the effect of nest parasites on birds with precocial young. More research is needed to determine what factors limit this flea population and whether fleas may become a regulating factor for geese in the Karrak Lake colony.