Thesis (M.Sc.) - University of KwaZulu-Natal, Pietermaritzburg, 2009. / Full text also available online. Scroll down for electronic link.

The distribution of flavonoid constituents in the barks of Acacia mearnsii De Wild.(black wattle) , A· decurens Willd. (green wattle), A. dealbata Link.(silver wattle) and A. pycnantha Benth.(golden wattle) has been studied. Bark extracts of the four wattle species have been fractionated into low molecular weight fractions containing mainly catechins and other low molecular weight constituents, and high molecular weight fractions containing the bulk of the polymerized tannins. The low molecular weight fractions have been further fractionated by "preparative paper chromatography". (-)-Robinetinidol, (-)-7:3': 4': 5'-tetrahydroxy flavan- 3-ol, a new naturally occurring catechin, (+)-catechin and (+)-gallocatechin have been isolated from the barks of A. mearnsii, A. dealbata and A. pycnantha. (-)-Epicatechin and (-)-epigallocatechin have been identified in the bark extracts of A. dealbata and A. pycnantha, but appeared to be absent in the barks of A. mearnsii and A. decurrens. (-)-Epicatechin has been isolated fron A. dealbata, and both (-}-epicatechin and (-)-epigallocatechin were isolated from A. pycnantha. (-)-Epicatechin gallate, (-)-epigallocatechin gallate and gallic acid were isolated from A. pycnantha only. These three constituents appeared to be absent in the barks of the three other wattle species. (-)-Epigallocatechin, (-)-epicatechin gallate and (-)-epigallocatechin gallate which were not available for direct comparison, were subsequently isolated from green tea where they are present as major phenolic constituents. A method for the quantitative estimation of polyphenolic substances on two dimensional paper chromatograms has been developed, and a photoelectric densitometer constructed. Two spray reagents, ammoniacal silver nitrate and bisdiazotised benzidine, were found to give straight line relationships of instrument deflection against log concentration for flavonoid substances. This estimation method for the first time supplied means for a detailed study of the concentration of catechin constituents in the bark extracts of A. mearnsii, A. decurrens, A. dealbata, A. pycnantha and of A. mearnsii x A. decurrens hybrids. The concentration of catechin constituents has been shown to vary considerably between species whereas variation within species was small. In the latter respect silver wattle is an exception. Taxonomic significance may possibly be attached to the distribution of catechin constituents in the bark of the four Acacia species. The concentration of (-)-robinetinidol, which appears to be the characteristic compound of these Acacias, progressively decreases in the sequence black-, black x green hybrid, green-, silver- and golden wattle, while the number of catechin constituents of the "phloroglucinol series" increases in the same sequence. It thus appears, that by the examination of their bark components, a differentiation between species of a subgenera may be possible. Two tannins, constituents D and B, which are related to the leuco-anthocyanidins (flavan-3:4-diols) have been found in the barks of the four wattle species. One of the two, constituent D, was isolated in a pure form from the barks of A. mearnsii and A. pycnantha. Constituent D was found to generate robinetinidin and an orange pigment, the structure of which has not yet been fully identified. Compound D and its acetyl- and methoxyl derivatives did not crystallize. From the results of alkaline-, acidic- and enzymatic degradations, colour reactions and light-absorption studies, combustion analysis of the compound and its derivatives and molecular weight estimations, constituent D is surmised to be a dimer of 7:3': 4': 5'-tetrahydroxyflavan-3:4-diol (leuco-robinetinidin), The isolation of this complex leuco-anthocyanidin tannin represents the first isolation of a flavonoid tannin from commercial vegetable tannin sources. The second tannin obtained from the bark of A.mearnsii, "constituent B" appears to consist of two overlapping substances, which have not yet been separated. The tannin (B) was found to have an average molecular weight of 676 and it is considered likely that both substances may be dimolecular. On heating with mineral acid robinetinidin, fisetinidin and an orange pigment are generated, the pigment being identical with the pigment generated from constituent D. It may therefore be assumed that "Constituent B" consists of a mixture of complex leuco-robinetinidins and leucofisetinidins. The distribution of complex leuco-anthocyanidins in the bark extracts of Acacia mearnsii, A. decurrens, A. dealbata and A. pycnantha has been examined. A correlation between the distribution of leuco-anthocyanidins in the bark of the four wattle species, and accepted systematics, does not, apparently, exist.

The impacts of invasive species on the environment, human health, and the economy continue to gain interest from public and private agencies, scientists, and the media. This study aimed to investigate the utility of SPOT 5 imagery and Artificial Neural Networks, in the identification and mapping of Acacia mearnsii within environments of varying complexity. Results showed that it is possible to identify and map Acacia mearnsii using SPOT 5 imagery, depending on the classification algorithm used. It was established that the neural network algorithms performed with greater success when compared to the performance of traditional classifiers, confirming other similar studies. The utility of the various classification algorithms was also investigated in terms of their applicability to environments of varying complexity. The neural networks once again, proved to be more successful and performed well in both the complex and relatively simple environments, indicating the robustness of the neural network algorithm. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.

Research into the micropropagation of black wattle in South Africa is important for two reasons. Firstly micropropagation technology allows breeders to select and propagate mature tissue, which in turn allows them to better capture selected traits. Secondly, tissue culture may control the highly invasive nature of black wattle. If triploid black wattle can be developed, foresters will then have to rely on clonal propagation to supply material for their growing operations. This research was part of the Institute for Commercial Forestry’s Acacia mearnsii vegetative propagation programme. The main focus of this research was to overcome various problems associated with direct organogenesis of ex vitro material. The shoot apex region was used as the explant in all studies because this region is thought to harbour relatively few internal microbial contaminants and is of sufficient size to withstand stresses associated with micropropagation. The initial research was focussed on the screening of sterilants, searching for a viable alternative to mercuric chloride. Surface sterilisation is integral to any micropropagation technique. This process should do the least amount of plant damage, whilst reducing microbial contamination to an acceptable level. Explants were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 BA and monitored for signs of contamination and shooting. Household bleach proved an excellent alternative to mercuric chloride because it did significantly less damage to the explants than mercuric chloride and is handled easily. There was no significant effect of sterilant exposure time on explant decontamination levels, whilst the shortest exposure time resulted in significantly higher levels of shoot development than the other two times tested. The results of this initial research was developed into a protocol and utilised in subsequent investigations. Due to a considerable variation in the success of the developed surface sterilisation protocol according to different times of the year, a further investigation into the effects of season and mother plant material on shoot apex culture of Acacia mearnsii was undertaken. The success of any tissue culture technique depends on a large array of ex vitro and in vitro variables. The objective of this research was to determine the ii effect of two ex vitro variables, season and mother plant, on shoot apex culture of Acacia mearnsii. Explants from individual mother plants were cultured on MS medium supplemented with 2.0 mg L-1 BA during four separate seasons and monitored for signs of contamination and shooting. Spring was found to be the best harvesting season because spring explants showed significantly higher decontaminated explant levels and shooting levels than explants harvested in the other three seasons. The effect of mother plant selection on the performance of Acacia mearnsii explants during shoot apex culture was also found to be significant, especially with regard to shooting levels. Finally factors influencing shoot elongation of A. mearnsii during shoot apex culture were investigated. In the past, induction of shoot elongation during micropropagation of A. mearnsii was attained through the addition of plant growth regulators and other supplements to the basal culture medium. However, some micropropagation methods in other species have utilised red light as a means of promoting shoot elongation. The objective of this study was to test the effects of an alternative basal medium, red light and differing concentrations of chemical additions to the culture medium on shoot elongation of Acacia mearnsii during shoot apex culture. Four independent experiments were undertaken comparing: shoot elongation on Woody Plant Medium (WPM) to the MS basal medium control; shoot elongation under a red cellophane box compared to control culture light conditions; shoot elongation on media supplemented with various concentrations of GA3 to the un-supplemented control and shoot elongation on media supplemented with combinations of BA and IBA compared to a control. Although no significant effects were observed, many trends were noted. The results indicated that there was no advantage to using WPM instead of MS medium when attempting to elongate shoots, rejuvenated through shoot apex culture of A. mearnsii, whilst the effect of GA3 showed a negative trend. The effects of red light and some BA and IBA combinations showed positive trends on the elongation of initiated shoots. This research successfully addressed some of the problems associated with micropropagation of A. mearnsii. Shoot apex culture shows promise and further research into this technique should be considered. A viable surface sterilant alternative to mercuric chloride was successfully identified. This alternative is not only iii safer to use but shows a large reduction in phytotoxic effects. The effects of season and mother plant on shoot apex culture was successfully investigated, resulting in a better understanding of mother plant influences on tissue culture as well as the identification of an optimum season for explant selection. Finally two possible shoot elongation promoters were identified for further research and a more affordable alternative to red light sources and screens was identified. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2007.

Acacia mearnsii (black wattle) is an important South African commercial forestry species, providing a source of high quality raw material (fibre) for both the domestic and international pulp and paper industries. Compared with many Pinus and Eucalyptus species, there has been very little research into the wood and pulping properties of black wattle. The ability to assess pulp yield in a non-destructive manner, using near infrared (NIR) spectroscopy, is vital from a tree improvement perspective. Destructive sampling and analysis, results in the loss of the genotype, while also being very expensive and time consuming. In order to assess some of the important characteristics that make the species desirable from a fibre perspective, this study investigates growth characteristics, wood density and pulp yield of ten trees grown on each of three different sites namely, Bloemendal, Glen Echo and Phoenix, and from each of three different age classes being 7- , 9- and 11-years-old. In total, 90 trees were sampled for this stage of the study. In general, physical characteristics such as utilisable height and diameter at breast height of the trees differed between sites and increased with age, this age effect trend was not reflected in the pulp yield or wood density results. Pulp yield measurements ranged from 52.61 to 59.91% across all sites and age classes, which, when compared to the pulp yield from many other forestry species, is relatively high. Laboratory pulp yield data was used in conjunction with NIR spectra obtained from the same wood samples to calibrate a NIR spectrophotometer to predict pulp yield. Thirty 11- year-old trees were then chosen from the Bloemendal site and sampled extensively to investigate the within-tree variation in pulp yield. The NIR model developed was used to measure the pulp yield from the numerous samples taken from within the trees. In general, pulp yield decreased from pith to bark and from the base of the tree to about 20% of the tree height and then decreased towards the top of the tree. The within-tree variation data for pulp yield was analysed to identify the best position for non-destructive sampling, and a model was then developed to predict whole tree pulp yield based on this sample, which was taken at 1.4 m up the tree. The analyses of small samples of wood meal, using near infrared spectroscopy, enabled the prediction of whole tree pulp yield. / Thesis (M.Sc.) - University of KwaZulu-Natal, Pietermaritzburg, 2009.

From Summary: The structural elucidation of condensed tannins, which are considered to consist of C₁₅-flavan units, presents certain difficulties. These tannins occur in extremely complex mixtures, and their isolation is complicated by their susceptibility to oxidative denaturation. Limitations in the formation of significant degradation products add to these problems. Since condensed tannins of black wattle bark yield anthocyanidins on treatment with mineral acids, they are considered to be proanthocyanidin in character. For this reason the initial approach to the investigation of the structures of condensed tannins was by way of the synthesis of novel 7-hydroxyflavan-3,4-diols having a low degree of hydroxyl substitution. Submission of these to modern physical techniques such as nuclear magnetic resonance (n.m.r.) spectroscopy would yield valuable data regarding the chemical shifts and coupling constants of heterocyclic and benzenoid protons which may be used for subsequent work on more highly hydroxylated diols, biflavanols and finally the condensed tannins themselves.

Made available in DSpace on 2014-08-20T13:25:40Z (GMT). No. of bitstreams: 1 Dissertacao_Alexandre_Terracciano_ Villela.pdf: 861318 bytes, checksum: 7eafbc8d87375d8a142a2b051afb0002 (MD5) Previous issue date: 2007-09-21 / The present work had as objective to evaluate the production of seedlings of black wattle (Acacia mearnsii of Wild), in substrata gotten from agro-industrial residues, composted and vermicomposted. After the search and the acquisition of the different residues used in the work, it was proceeded the disposal from these isolated materials and in not aromatical combination in boxes wooden (cedrinho) in order to carry through the composting process later and the vermcomposting. After the vermicomposting to be concluded preceded it mixture from the materials including itself it carbonized rind of rice (CAC). The treatments were: T1 (bovine manure (EB) 50%+ rind of parboilizado rice (CAC) 50%); T2 (ovine manure (EO) 50%+CAC 50%); T3 (agroindustrial residues (FROG) 50%+CAC 50%); T4 (mud of parborizado rice (LP) 50%+CAC 50%); T5 (bovine manure(EB) 25%+LP 25%+CAC 50%); T6 (EO 25%+LP 25%+CAC 50%); T7 (EB 25%+RA 25%+CAC 50%; T8- EO 25%+RA 25%+CAC 50%); T9 (EB 25%+RF 25%+CAC 50%); T10 (EO 25%+RF 25%+CAC 50%) and T11 (Plantmax®). The evaluated variable had been: number of plants, col diameter, height of plants, aboveground biomass (wet and dry), root fitomass (wet and dry), total foliar area, chemistry composition od seedlings and chemistry composition of the substrata before and after the research. The sowing occurred in rigid the first day of June of 2006 in tubetes (1320 units) with capacity conditioning 50 approximately cm3 of substratum. The seeds was concluded the germinative process in day 25 of the same month. When the changes had reached the height of two centimeters, proceded the looping, leaving itselves only one seedlings one for container. During the experiment the changes had been irrigated with the frequency of 6L m-2 day-1, until the 45 days of the plantation. From the 46 days 12L was changed for two irrigations totalizing m-2 day-1, due to rise of the local temperature remaining this frequency until the end of the evaluations in greenhouse. To the twenty and three days of the month of September of 2006 (115 days to count of the sowing), the ending of the evaluations in greenhouse was given and all the changes had been removed and directed to the laboratory to be carried through the too much evaluations. The gotten results had been submitted to the variance analysis, test of averages (Tukey 5%), where it was possible to evidence that the T11 treatment with commercial substratum Plantmax®, presented the best answers for seedlings of black wattle (acácia mearnsii of Wild). / O presente trabalho teve como objetivo avaliar a produção de mudas de acácia negra (Acacia mearnsii De Wild), em substratos obtidos a partir de resíduos agroindustriais, compostados e vermicompostados. Após a busca e a aquisição dos diferentes resíduos utilizados no trabalho, procedeu-se à disposição desses materiais isolados e em combinação em caixas de madeira não aromática (cedrinho) a fim de realizar o processo de compostagem e posteriormente a vermicompostagem. Após a vermicompostagem estar concluída procedeu-se a mistura dos materiais incluindo-se a casca de arroz carbonizada (CAC) que compuseram os seguintes tratamentos: T1(esterco bovino (EB) 50%+ casca de arroz parboilizado (CAC) 50%); T2(esterco ovino (EO) 50%+CAC 50%); T3(restos da agroindustria (RA) 50%+CAC 50%); T4(lodo de arroz parborizado (LP) 50%+CAC 50%); T5(esterco bovino (EB) 25%+LP 25%+CAC 50%); T6(EO 25%+LP 25%+CAC 50%); T7(EB 25%+RA 25%+CAC 50%; T8- EO 25%+RA 25%+CAC 50%); T9(EB 25%+RF 25%+CAC 50%); T10(EO 25%+RF 25%+CAC 50%) e T11(Plantmax®). As variáveis avaliadas foram: diâmetro de colo, altura de plantas, fitomassa fresca da parte aérea, fitomassa fresca da raiz, fitomassa seca da parte aérea, fitomassa seca da raiz, área foliar total, composição química das mudas e dos diferentes substratos antes e após a retirada das mesmas. A semeadura ocorreu no dia primeiro de junho de 2006 em tubetes rígidos (1320 unidades) com capacidade de acondicionar aproximadamente 50 cm3 de substrato. O processo germinativo foi concluído no dia 25 do mesmo mês. Quando as mudas atingiram a altura de dois centímetros, procedeu-se o desbaste, deixando-se apenas uma muda por recipiente. Durante o experimento as mudas foram irrigadas com a freqüência de 6L m-2 dia-1, até os 45 dias do plantio. A partir dos 46 dias mudou-se para duas irrigações totalizando 12L m-2 dia-1, devido à elevação da temperatura local mantendo-se essa freqüência até o final das avaliações em estufa. Aos vinte e três dias do mês de setembro de 2006 (115 dias a contar da semeadura), deu-se o término das avaliações em estufa e todas as mudas foram retiradas e encaminhadas ao laboratório para serem realizadas as demais avaliações. Os resultados obtidos foram submetidos à análise de variância, teste de médias (Tukey 5%), onde foi possível constatar que o tratamento T11 com substrato comercial Plantmax®, apresentou as melhores respostas agronômicas para mudas de acácia negra (acácia mearnsii de Wild).

Les plantes invasives constituent un phénomène très répandu sur la planète qui entraîne des problèmes environnementaux majeurs aboutissant à des perturbations significatives dans les processus régissant la conservation de la diversité végétale et microbienne des sols. Acacia mearnsii (De Wild) est l'une des espèces d'Acacia ayant un potentiel invasif important. Cette légumineuse d'origine australienne a été introduite dans les subéraies du Parc National d'El-Kala (PNEK), au Nord-Est de l'Algérie, où elle induit des dégradations drastiques sur l'écosystème forestier de chêne-liège Quercus suber (L.) et la diversité végétale et microbienne de cette formation forestière. L'objectif de cette étude a été d'évaluer les transformations induites par A. mearnsii sur les caractéristiques et microbienne du sol (particulièrement les communautés de symbiotes microbiens) et d'en déterminer les conséquences sur le développement de chêne-liège. Une série d'expérimentations a été réalisée sur les sols de trois sites dans le PNEK : forêt naturelle de Q. suber (site non envahi), peuplement mixte Q. suber et A. mearnsii (site envahi récemment par A. mearnsii) et peuplement d'A. mearnsii (site anciennement envahi par A. mearnsii). Les analyses de sol, de la diversité fonctionnelle et structurelle des microorganismes telluriques ont révélé l'existence de modifications liées à la présence de l'espèce envahissante. D'autre part, il a été déterminé que A. mearnsii avait un effet inhibiteur sur le développement du chêne-liège et sur son cortège ectomycorhizien associé. De plus, cette essence semble avoir la capacité de s'adapter facilement au milieu d'introduction, du fait de sa forte mycotrophie vis à vis des champignons mycorhiziens arbusculaires, et de sa facilité de nodulation spontanée avec des bactéries symbiotiques principalement du genre Bradyrhizobium. La présence de symbiotes compatibles avec A. mearnsii dans les habitats envahis représente probablement un des facteurs susceptibles de faciliter le processus d'envahissement de l'espèce. Nos résultats suggèrent que la régression du développement du chêne-liège peut être liée aux modifications induites par A. mearnsii sur le fonctionnement du sol et la structure des microorganismes telluriques. Cet effet est probablement lié à d'autres éléments biotiques et abiotiques du milieu envahi influencé par cet arbre. Il sera donc important d'affiner cette étude, et d'analyser plus précisément les paramètres pouvant être à l'origine du succès de l'invasion par A. mearnsii afin de définir un cadre de lutte contre cette espèce invasive et ainsi sauvegarder la subéraie . / Invasive plants are a global phenomenon causing major environmental problems leading to significant disruptions in the processes governing the conservation of plant diversity and soil microbial communities. Acacia mearnsii (De Wild) is one of the Acacia species with a significant invasive potential. This Australian native legume was introduced in the cork forests of the National Park of El Kala (PNEK), North-eastern Algeria, and induced drastic degradation of the cork oak (Quercus suber (L.)) forest ecosystem, on understorey plant species and soil microbial diversity. The objective of this study was to evaluate changes resulting from the A. mearnsii invasion on chemical characteristics and soil microbiota (especially symbiotic microbial communities) and to determine their impact on cork oak development. Experiments were conducted on soils collected from three sites in the PNEK: Q. suber natural stand (non-invaded site), mixed Q. suber and A. mearnsii stand (recently invaded site) and A. mearnsii stand (fully invaded site). Both chemical and microbiological soil characteristics were affected by the presence of the invasive species. On the other hand, it was determined that A. mearnsii had an inhibitory effect on the development of the cork oak tree and its associated ectomycorrhizal community. In addition, this tree species seems to easily interact with arbuscular mycorrhizal fungi in the introduction area, being abundantly infected. It is also spontaneously nodulated by local symbiotic bacteria, mainly of the genus Bradyrhizobium. The presence of symbionts, compatible with A. mearnsii in invaded habitats are probably one of the factors that facilitate the process of invasion. Our results suggest that the development of the cork oak can be linked to changes induced by A. mearnsii with soil functioning and with the composition of soil microorganism communities. This effect is probably related to other biotic and abiotic components of the environment influenced by this invasive tree species. It seems thus important to analyze more precisely the parameters that cause the success of the invasion by A. mearnsii in order to better control this invasive species and save the cork oak forest in Algeria.

Leaf area index (LAI) is an important variable in models that attempt to simulate carbon, nutrient, water and energy fluxes for forest ecosystems. LAI can be measured either directly (destructive sampling) or by using indirect techniques that involve estimation of LAI from light penetration through canopies. Destructive sampling techniques are laborious, expensive and can only be carried out for small plots. Although indirect techniques are non-destructive and less time consuming, they assume a random foliage distribution that rarely occurs in nature. Thus a technique is required that would allow for rapid estimation of LAI at the stand level. A means of getting this information is via remotely sensed measurements of reflected energy with an airborne or satellite-based sensor. Such information on an important plant species such as Acacia mearnsii (Black Wattle) is vital as it provides an insight into its water use. Landsat ETM+ images covering four study sites In KwaZulu-Natal midlands encompassing pure stands of Acacia mearnsii were processed to obtain four types of vegetation indices (VIs). The indices included: normalized difference vegetation index (NDVI), ratio vegetation index (RVI), transformed vegetation index (TVI) and vegetation index 3 (VB). Ground based measurements of LAI were made using destructive sampling (actual LAI) and LAI-2000 optical instrument, (plant area index, PAl). Specific leafarea (SLA) and leaf area (LA) were measured in the field for the entire sample stands to estimate their LAI values. The relationships between the various VIs and SLA, actual LAI and PAl values measured by LAI-2000 were evaluated using correlation and regression statistical analyses. Results showed that the overall mean SLA value of Acacia mearnsii was 8.28 m2kg-1 SLA showed strong correlations with NDVI (r=0.71, p<O.Ol) and RVI (r=0.76, p<O.Ol) and a moderate correlation with TVI (r=0.66, p<0.05). Regression analysis revealed that SLA had significant relationship with RVI (R2=0.59) and NDVI (R2=0.51). Actual LAI values showed strong correlation with PAl values (r=0.86) and the analysis revealed that 74 % of the variation in the relationship between actual LAI and PAl values could be explained by regression. PAl values were strongly correlated with NDVI (r=0.75,p<O.Ol) and moderately correlated with RVI (r=O.63, p<O.05) and TVI (r=O.58, p<O.05). Actual LAI was strongly correlated with NDVI (r=O.79, p<O.Ol) and moderately correlated with RVI (r=O.61, p<O.05). Out of the various VIs examined in this study, NDVI was found to have a better relationship with actual LAI values (R2=O.62) and with PAI values (R2=O.56); while VB didn't show any significant relationship with SLA, PAl or actual LAl. In conclusion, preliminary estimate of SLA of Acacia mearnsii could be obtained from RVI or NDVl. The relationship obtained between PAl and actual LAI values was satisfactory, thus the regression equation can be used to calibrate the LAI-2000 plant canopy analyzer. Because NDVI was observed to have a good relationship with actual LAI and PAl, LAI of Acacia mearnsii can be estimated from Landsat ETM+ satellite imagery with a reasonable degree of accuracy. These results can satisfactorily be used as inputs into models that attempt to estimate water use by Acacia mearnsii. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2003.

Submitted by PPG Engenharia e Tecnologia de Materiais (engenharia.pg.materiais@pucrs.br) on 2018-11-16T17:04:36Z No. of bitstreams: 1 Disserta??o homologa??o Viviane Bettanin 14.11.2018.pdf: 3436850 bytes, checksum: 12199a52b8023657a598a8254ca85c0f (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-11-19T13:58:36Z (GMT) No. of bitstreams: 1 Disserta??o homologa??o Viviane Bettanin 14.11.2018.pdf: 3436850 bytes, checksum: 12199a52b8023657a598a8254ca85c0f (MD5) / Made available in DSpace on 2018-11-19T14:07:36Z (GMT). No. of bitstreams: 1 Disserta??o homologa??o Viviane Bettanin 14.11.2018.pdf: 3436850 bytes, checksum: 12199a52b8023657a598a8254ca85c0f (MD5) Previous issue date: 2018-08-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / In order to reduce the environmental impacts caused by chemical harmful to the environmental and to human health, the development of natural substances that are able to substitute these dangerous components have been increased. Tannins are a group of natural poliphenolic components extracted from plants that could be used as corrosion inhibitors. This study investigates the utilization of two bark of black wattles (Acacia mearnsii) as corrosion inhibitors, Weibull and Macrospec, in acidic medium and in atmospheric environment and in aqueous CO2 rich medium under high pressure. API 5L X65 steel samples were exposed to a corrosive medium (0.1 M HCl) with different tannin concentrations. The best results were duplicate to an environmental with CO2 saturated water at 15 MPa and 50?C. In order to characterize the specimens scanning electron microscopy by gun field emission, energy dispersive spectroscopy, X ray diffraction, mass loss tests and potenciodynamic polarization tests were used. The results indicated that both tannins, at atmospheric pressure and 0.1 M HCl corrosive environment have similar performance as corrosion inhibitors, and the higher dosage tested (12 g/L) exhibited the best efficiency. However, when the tannins were tested in environment with presence of CO2 under high pressure (15 MPa) and 50?C the results were different, and only Macrospec tannin had an inhibitory action under the conditions tested. / Visando diminuir os impactos ambientais causados por subst?ncias qu?micas nocivas ao meio ambiente e ? sa?de humana, a procura por substitutos naturais destes compostos em diversas aplica??es vem sendo intensificada. Taninos compreendem uma classe de compostos polifen?licos naturais extra?dos de plantas, podendo ser usados como inibidores de corros?o em meio aquoso. Neste estudo avaliou-se a utiliza??o de dois taninos vegetais da ac?cia negra (Acacia mearnsii), Weibull (em p?) e Macrospec (l?quido), como inibidores de corros?o em meios ?cidos em ambiente atmosf?rico e em ambiente com a presen?a de CO2 a alta press?o. Corpos de prova de a?o API5L X65 foram expostos a meio acidificado com HCl 0,1 M com diferentes concentra??es de taninos e o melhor resultado no que se refere ? concentra??o do tanino para a inibi??o de corros?o foi replicado para um meio com ?gua saturada com CO2 a 15 MPa e 50?C. Para caracteriza??o foram realizadas as an?lises de microscopia eletr?nica de varredura, difra??o de raios X, medidas eletroqu?micas de polariza??o potenciodin?mica, e testes de perda de massa. Os resultados indicaram que ambos os taninos, em meio de HCl 0,1 M e press?o atmosf?rica, apresentaram desempenho similar no que diz respeito aos efeitos de inibi??o da corros?o e a maior dosagem utilizada (12 g/L) foi a que apresentou melhor efici?ncia de inibi??o. Por?m, quando testados em meio de ?gua saturada com CO2 a 50?C e 15 MPa o comportamento dos taninos foi distinto, sendo que apenas o tanino Macrospec teve a??o inibit?ria nas condi??es testadas.